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| Impacts of miR-199a-5p on Glycolysis and Collagen Synthesis in Keloid Fibroblasts by Regulating PKM2 |
| LIU Yulan, GONG Lixin, YU Daojiang, et al |
| The Second Affiliated Hospital of Chengdu Medical College, Nuclear Industry 416 Hospital, Sichuan Chengdu 610000, China |
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Abstract Objective: To explore the impacts of microRNA-199A-5p (miR-199a-5p) on glycolysis and collagen synthesis in keloid fibroblasts (KFB) by regulating pyruvate kinase isozyme type M2 (PKM2). Methods: The dual luciferase reporter gene assay was used to examine the interaction between miR-199a-5p and PKM2. KFB was assigned into Control group, NC-mimics group, miR-199a-5p-mimics group, miR-199a-5p-mimics+OE-NC group, and miR-199a-5p-mimics+OE-PKM2 group. The qRT-PCR method was used to measure miR-199a-5p and PKM2 mRNA in KFB. CCK8 was used to measure KFB proliferation. The reagent kits were used to measure glucose consumption and lactate production. Flow cytometry was used to detect apoptosis of KFB. Western blot was used to detect type I collagen (Col-I), type III collagen (Col-III), and PKM2 protein in KFB. Results: MiR-199a-5p could target negative regulation of PKM2(t = 14.394, P<0.001). The miR-199a-5p-mimics group had higher apoptosis rate and miR-199a-5p, and lower A450 value, glucose consumption, lactate production, PKM2 mRNA and protein, Col-Ⅰ, and Col-Ⅲ than Control group and NC-mimics group (P<0.05). The miR-199a-5p-mimics+OE-PKM2 group had higher A450 value, glucose consumption, lactate production, PKM2 mRNA and protein, Col-Ⅰ, Col-Ⅲ, and lower apoptosis rate than the miR-199a-5p-mimics group and the miR-199a-5p-mimics+OE-NC group (P<0.05). Conclusion: MiR-199a-5p may regulate PKM2 to inhibit glycolysis and collagen synthesis in KFB.
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2025, Vol. 31 
