汉黄芩素调节Wnt/β-catenin信号通路对慢性肾脏病大鼠血管钙化的影响

doi:10.3969/j.issn.1006-6233.2025.08.05

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Abstract Objective: To investigate the impact of wogonin (WOG) on vascular calcification in chronic kidney disease (CKD) rats by regulating the Wnt/β-catenin signaling pathway. Methods: Nine rats were stochastically selected as control group, while the rest were constructed as CKD models. After successful treatment, they were assigned into CKD group, WOG+CKD group, and WOG+CKD+activator group, with nine rats in each group. After 8 weeks of administration, biochemical indicators such as blood urea nitrogen (BUN), creatinine (SCr), serum calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), and whole parathyroid hormone (iPTH) were detected in the serum of rats in each group. Von Kossa staining was performed to detect aortic calcification. Immunohistochemical staining was performed to detect Runt related transcription factor 2 (Runx2) in aortic tissue. RT-qPCR was performed to detect the mRNA expression of Wnt1 and β-catenin in rat aortic tissue. In addition, Western blot was used to detect the expression of Wnt1 and β-catenin proteins in rat aortic tissue. Results: Compared with the control group, CKD group showed increased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, severe aortic calcification, and elevated Wnt1 and β-catenin mRNA and protein expression (P<0.05). Compared with the CKD group, the WOG+CKD group showed decreased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, reduced aortic calcification, and decreased Wnt1 and β-catenin mRNA and protein expression (P<0.05). Compared with the WOG+CKD group, the WOG+CKD+activator group showed increased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, worsened aortic calcification, and elevated Wnt1 and β-catenin mRNA and protein expression (P<0.05). Conclusion: WOG may alleviate vascular calcification in CKD rats by inhibiting the Wnt/β-catenin signaling pathway.

Key words： Wogonin Chronic kidney disease rats Vascular calcification Wnt/β-catenin signaling pathway

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.08.05 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I8/1258

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