脑膜瘤组织中脆性X相关基因1过表达促进脑膜瘤细胞生长与血管生成的研究

doi:10.3969/j.issn.1006-6233.2021.06.07

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Abstract Objective: To investigate the expression of RNA-binding gene fragile X-related protein 1 (FXR1) in human meningiomas and its effect on the growth and angiogenesis of meningiomas. Methods: Collect the tumor tissue and adjacent tissue specimens of 24 meningiomas in our hospital, and detect the expression of FXR1 by immunohistochemical staining. Isolate and culture human meningioma cells, and divide the cultured cells into blank control group, pcDNA3.1-NC group and pcDNA3.1-FXR1 group. Transfect the pcDNA3.1-FXR1 plasmid vector and the pcDNA3.1 empty plasmid vector to meningiomas by lipofection; Real-time fluorescent quantitative PCR and Western blot detection of FXR1 mRNA and protein expression levels in cells were pweformed; CCK-8 experiment was conducted to detect cell proliferation activity; cell clone formation test was conducted to detect cell colony forming ability; Western blot was conducted to detect VEGF and EDN-1 protein expression levels; flow cytometry was conducted to detect the positive cell expression of the markers CD31 and CD34 of vascular endothelial cells; in vitro tubule formation test was conducted to detect the tube formation of HUVECs. Results: The positive expression of FXR1 in meningioma tissue was significantly higher than that in adjacent tissues (P<0.05). Compared with the blank control group and the pcDNA3.1-NC group, the relative expression of FXR1 mRNA and protein in the cells of the pcDNA3.1-FXR1 group were significantly increased (P<0.05), cell proliferation activity increased significantly after 48, 72 and 96 hours of transfection (P<0.05), the number of cell clone formation increased significantly (P<0.05), VEGF and EDN-1 protein expression levels increased significantly (P<0.05), the number of positive cells marked by CD34 and CD31 increased significantly (P<0.05), at the same time, the number of lumens formed increased significantly. Conclusion: FXR1 is highly expressed in human meningiomas. Overexpression of FXR1 can promote meningioma cell growth and angiogenesis.

Key words： Meningiomas Fragile X-related gene 1 Proliferation Angiogenesis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.06.07 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I6/910

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