|
|
|
| Correlation between miR-762 and sTREM-1 sFLT-1 in Acute Lung Injury |
| SONG Yang, et al |
| Beijing Geriatrics Hospital, Beijing 100095, China |
|
|
|
|
Abstract Objective: To observe the expression of miR-762 in plasma of patients with acute lung injury and explore its relationship with potential value.Methods: A total of 60 patients with lung injury admitted to our hospital from September 2018 to September 2021 were collected and divided into medium low risk group and high risk group according to clinical symptoms and mMRC scores. Scores were analyzed with acute physiology and chronic health evaluation (APACHE Ⅱ). Human soluble myeloid cell trigger receptor-1 (sTREM-1) kit and human soluble FMS like tyrosine kinase 1 (sFlt-1) enzyme linked immunosorbent assay (ELISA) kit were used to detect the contents of sTREM-1, sFlt-1 in plasma; real time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-762 in plasma. Receiver operating characteristic curve (ROC) was plotted to judge the diagnostic value and cut-off point of miR-762 in patients with lung injury. Results: The contents of sTREM-1 and sFlt-1, the relative expression of miR-762 of high-risk group and middle-low-risk group were all significantly higher than those of normal group. Compared with the middle-low-risk group, the contents of sTREM-1, sFlt-1 and the relative expression of miR-762 were substantially increased in the high-risk group in plasma. ROC curve showed that the relative expression of miR-762 was used to predict the area under curve (AUC) = 0.987, 95% confidence interval (CI): (0.9688 - 1.000), and the cut-off point was 1.210; AUC = 1.00, 95% CI: (1.000-1.000), with a cut-off point of 2.396. The scores of miR-762 and APACHE Ⅱ markedly increased, and the contents of sTREM-1 and sFlt-1 were positively correlated. The correlation coefficients (r) were 0.386, 0.321, 0.432. Conclusion: The high expression of miR-762 in plasma from patients with acute lung injury was related to the aggravation of lung injury, and it was a predictive marker of acute lung injury.
|
|
|
|
|
|
[1] Butt Y,Kurdowska A,Allen TC.Acute lung Injury: a clinical and molecular review[J].Arch Pathol Lab Med,2016,140(4): 345-350.
[2] Mowery NT,Terzian WTH,Nelson AC.Acute lung injury[J].Curr Probl Surg,2020,57(5): 100777.
[3] Mokra D,Mikolka P,Kosutova P,et al.Corticosteroids in acute lung injury: the dilemma continues[J].Int Mol Sci,2019,20(19): 4765.
[4] D'Alessio FR.Mouse models of acute lung injury and ARDS[J].Methods Mol Biol,2018,1809(7): 341-350.
[5] Wang C,Sun K,Wang J,et al.Data fusion-based algorithm for predicting miRNA-disease associations[J].Comput Biol Chem,2020,88(10): 107357.
[6] Ge P,Cao L,Chen X,et al.miR-762 activation confers acquired resistance to gefitinib in non-small cell lung cancer[J].BMC Cancer,2019,19(1): 1203.
[7] Yan K,An T,Zhai M,et al.Mitochondrial miR-762 regulates apoptosis and myocardial infarction by impairing ND2[J].Cell Death Dis,2019,10(7): 500.
[8] Hu C,Jiang J,Li Z,et al.Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with acinetobacter baumannii colonization and infection in the lung[J].Thorac Dis,2018,10(3): 1614-1621.
[9] Syed MA,Shah D,Das P,et al.TREM-1 Attenuates RIPK3-mediated necroptosis in hyperoxia-induced lung Injury in neonatal mice[J].Am Respir Cell Mol Biol,2019,60(3): 308-322.
[10] Zhuang C,Piao C,Choi M,et al.Delivery of MiRNA-92a inhibitor using RP1-linked peptide elicits anti-inflammatory effects in an acute lung injury model[J].Biomed Nanotechnol,2021,17(7): 1273-1283.
[11] Zhang W,Li J,Yao H,et al.Restoring microRNA-499-5p protects sepsis-induced lung injury mice via targeting sox6[J].Nanoscale Res Lett,2021,16(1): 89. |
|
|
|
2022, Vol. 28 
