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| Effect of Baicalin on High-Glucose Induced Apoptosis of Myocardial Cells by Regulating the SDF-1/CXCR4 Signaling Pathway |
| GUO Jing, YANG Juhong, ZHANG Lin, et al |
| Jingmen Central Hospital, Hubei Jingmen 448124, China |
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Abstract Objective: To investigate the effect of baicalin on high glucose (HG) induced apoptosis of myocardial cells by regulating the stromal cell derived factor 1 (SDF-1)/chemokine receptor 4 (CXCR4) signaling pathway.Methods: H9C2 myocardial cells were used as the research object and grouped into control group, HG group, low concentration baicalin group (L-Ba group), medium concentration baicalin group (M-Ba group), high concentration baicalin group (H-Ba group), and baicalin+CXCR4 inhibitor group (Ba+AMD3100 group). CCK-8 method was applied to detect cell viability in each group; flow cytometry was applied to detect the apoptosis rate of cells in each group; qRT-PCR was applied to detect the mRNA expression of Bax and Bcl-2 of cells in each group; microplate method was applied to detect SOD activity and content of MDA of cells in each group; Western blot (WB) was applied to detect the expression levels of SDF-1 and CXCR4 proteins of cells in each group.Results: Compared with the control group, the survival rate, relative expression of Bcl-2 mRNA, SOD activity, and expression levels of SDF-1 and CXCR4 proteins in the HG group decreased, the apoptosis rate of myocardial cells, relative expression of Bax mRNA, and content of MDA increased (P<0.05); compared with the HG group, the survival rate, relative expression of Bcl-2 mRNA, SOD activity, and expression levels of SDF-1 and CXCR4 proteins in the L-Ba group, M-Ba group, H-Ba group increased, the apoptosis rate of myocardial cells, relative expression of Bax mRNA, and content of MDA decreased, and showed gradual upward or downward trend as the concentration of baicalin increased (P<0.05); the survival rate of myocardial cells, relative expression of Bcl-2 mRNA, SOD activity, and protein expression levels of SDF-1 and CXCR4 in the Ba+AMD3100 group were lower than those in the L-Ba, M-Ba, and H-Ba groups, the apoptosis rate of myocardial cells, relative expression of Bax mRNA, and content of MDA were higher in the L-Ba group, M-Ba group, and H-Ba group (P<0.05).Conclusion: Baicalin can alleviate HG-induced apoptosis of myocardial cells by activating the SDF-1/CXCR4 signaling pathway.
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2024, Vol. 30 
