LncRNA LBX2-AS1调节miR-873-5p/G6PD轴对胃癌细胞增殖迁移和侵袭的影响

doi:10.3969/j.issn.1006-6233.2024.09.014

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Abstract Objective: To investigate the effects of long non-coding RNA (LncRNA) ladybird homeobox 2 antisense RNA1 (LBX2-AS1) on the proliferation, migration, and invasion of gastric cancer (GC) cells by regulating the micro RNA-873-5p (miR-873-5p)/glucose-6-phosphate dehydrogenase (G6PD) axis.Methods: SGC7901 cells were randomly separated into control group, sh-NC group, sh-LBX2-AS1 group, sh-LBX2-AS1+inhibitor NC group, and sh-LBX2-AS1+miR-873-5p inhibitor group. qRT-PCR method was applied to detect the expression of LncRNA LBX2-AS1, miR-873-5p, and G6PD. MTT method and plate cloning experiment were applied to detect the proliferation of SGC7901 cells. Scratch experiment was applied to detect the migration of SGC7901 cells. Transwell experiment was applied to detect the invasion of SGC7901 cells. Western blot was applied to detect the expression of MMP-2, PCNA, MMP-9, and G6PD proteins in SGC7901 cells. Luciferase reporter gene assay was applied to detect the interaction between LncRNA LBX2-AS1 and miR-873-5p, and between miR-873-5p and G6PD. Mouse tumor bearing experiment was applied to verify the effects of knocking out LncRNALBX2-AS1 on CC tumor growth and G6PD expression.Results: The A490 value, clone number, scratch healing rate, invasion number, LncRNA LBX2-AS1, G6PD mRNA and PCNA, MMP-2, MMP-9 protein expression in SGC7901 cells in sh-LBX2-AS1 group were lower than those in sh-NC group and control group, while miR-873-5p expression was higher than that in sh-NC group and control group (P<0.05). Compared with the sh-LBX2-AS1 group and the sh-LBX2-AS1+inhibitor NC group, the sh-LBX2-AS+miR-873-5p inhibitor group showed a decrease in miR-873-5p expression, and an increase in A490 value, clone number, scratch healing rate, invasion number, G6PD mRNA and PCNA, MMP-2, MMP-9 protein expression (P<0.05). LncRNA LBX2-AS1 targeted the negative regulation of miR-873-5p, and miR-873-5p targeted the negative regulation of G6PD. The quality, volume, Ki-67 positive rate, and G6PD positive rate of transplanted tumors in the experimental group were lower than those in the control group (P<0.05).Conclusion: Knocking out LncRNA LBX2-AS1 may inhibit the proliferation, migration, and invasion of GC cells, and its mechanism may be achieved by regulating the miR-873-5p/G6PD axis.

Key words： Long non-coding RNA Ladybird homeobox 2 antisense RNA1 Micro RNA-873-5p Glucose-6-phosphate dehydrogenase (G6PD) Gastric cancer Proliferation Migration Invasion

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.09.014 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I9/1488

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