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| Effects of Different Concentrations of iPRF on Melanocyte Culture |
| SUN Shuhong, HU Yuanjing, JI Hailian, et al |
| Chengde Maternity and Child Health Care Hospital, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the effects of different concentrations of injectable platelet rich fibrin (iPRF) on the migration, activation, proliferation and tumorigenicity of melanocytes. Methods: Control group, experimental group 1 and experimental group 2 were configured with no iPRF, iPRF concentration of 1/3, iPRF concentration of 1/2, respectively. The melanocyte migration, cell proliferation, melanin concentration and tumorigenicity were compared between groups. Results: Comparison of the three culture systems, the migration of melanocytes was the most obvious in experimental group 2, followed by experimental group 1, and the least in control group. There was no statistical difference in the proliferation of melanocytes between groups (P>0.05). The amount of melanin was the highest in the experimental group 1, and there was no statistical difference between the control group and the experimental group 2 (P>0.05). In the three culture systems, the three groups showed a dose-dependent downregulation of colony formation rate of melanocyte, but the number of clones in each group was less than 50, and the clone plate test was negative. Conclusion: Different culture systems have different migration and activation effects on melanocytes. The migration and activation effect of melanocyte showed a downward trend with the increase of iPRF concentration. iPRF may promote the production of melanin, but not the higher the concentration, the stronger the effect. iPRF did not promote the proliferation of melanocytes and had no tumorigenic effect.
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[1] Khattab Fathia M, Abdelbary Eman, Fawzi Maggie.Evaluation of combined excimer laser and platelet-rich plasma for the treatment of nonsegmental vitiligo:a prospective comparative study[J].Journal of Cosmetic Dermatology,2020,19(4):869-877.
[2] Tedesco M.Platelet-rich plasma for genital lichen sclerosus:analysis and results of 94 patients.Are there gender-related differences in symptoms and therapeutic response to PRP[J].Dermatolog Treat,2022,33(3):1558-1562.
[3] Medina Garrido C.Mid-term symptomatic relief after platelet-rich plasma infiltration in vulvar lichen sclerosus[J].Arch Dermatol Res,2023,315(6):1527-1532.
[4] Shashank B, M.Bhushan.Injectable platelet-rich fibrin (PRF):The newest biomaterial and its use in various dermatological conditions in our practice:a case series[J].Cosmet Dermatol,2021,20(5):1421-1426.
[5] Varela H A.Injectable platelet rich fibrin:cell content,morphological,and protein characterization[J].Clin Oral Investig,2019.23(3):1309-1318.
[6] Monitha Gollapudi,Pavan Bajaj,Ranu R Oza.Injectable platelet-rich fibrin - A revolution in periodontal regeneration[J].Cureus,2022,14(8):28647.
[7] Meng Li, Yibin Fan, Yutong Wang.ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo[J].Experimental and Therapeutic Medicine,2020,20(2):1371-1378.
[8] Amanda Haage,Kelsey Wagner,Wenjun Deng,et al.Precise coordination of cell-ECM adhesion is essential for efficient melanoblast migration during development[J].Development,2020,147(14):184234.
[9] 李哲曦,王大光.真表皮间黑素细胞转换与移行的调节机制[J].中国皮肤性病学杂志,2024,38(9):945-950. |
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2025, Vol. 31 
