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| Effect of Ophiopogonin-B on Malignant Progression of Breast Cancer Cells by Regulating PD-1/PD-L1 Signaling Pathway |
| LI Shifen, JIANG Jianping, CHEN Hong |
| Pengzhou People's Hospital, Sichuan Pengzhou 611930, China |
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Abstract Objective: To investigate the effect and mechanism of different concentrations Ophiopogonin-B (OP-B) on the malignant behavior of breast cancer cells. Methods: Human breast cancer cells were inducted into control group, OP-B low, medium and high concentration groups (transfected with 10, 20 and 40 μmol/L OP-B), high concentration OP-B+pcDNA-NC group (transfected with pcDNA-NC and 40 μmol/L OP-B ), and high concentration OP-B+pcDNA-PD-1 group (transfected with pcDNA-PD-1 and 40 μmol/L OP-B ). The proliferation ability, the migration and invasion abilities, and the apoptosis rate of MCF-7 cells were detected by cell counting kit-8 (CCK-8) assay, Transwell and Flow cytometry, respectively. Western blot was used to detect the protein expression levels of programmed death-1 (PD-1), programmed death-ligand 1 (PD-L1), vascular endothelial growth factor (VEGF), and transforming growth factor beta1 (TGF-β1) of MCF-7 cells in each group. Results: Compared with the control group, the proliferation rate, migration and invasion ability of MCF-7 cells in each treatment group of OP-B were significantly reduced (P<0.05), and the apoptosis rate was significantly increased (P<0.05), the protein expression levels of PD-1, PD-L1, VEGF and TGF-β1 in MCF-7 cells were significantly reduced (P<0.05). OP-B could significantly inhibit the malignant behavior and immune escape of MCF-7 cells, showing a concentration dependent (P<0.05), however, pcDNA-PD-1 plasmid transfection could significantly reverse the effect of high concentration OP-B on MCF-7 cells (P<0.05). Conclusion: OP-B may reduce the expression of VEGF and TGF-β1 by inhibiting the PD-1/PD-L1 pathway, thereby reducing proliferation, migration, and invasion of MCF-7 cell, promoting cell apoptosis, and reducing the occurrence of immune escape.
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2025, Vol. 31 
