白芦藜醇对大鼠牙移植后牙周组织愈合及Sirt1/Nrf2信号通路的影响

doi:10.3969/j.issn.1006-6233.2022.11.06

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Abstract Objective: To observe the influences of resveratrol (RSV) on the periodontal tissue healing and Sirt1/Nrf2 signaling pathway after tooth transplantation in rats.Methods: 48 male Lewis rats were randomly divided into control (Control) group, model (Model) group, low-dose RSV (L-RSV) group, and high-dose RSV (H-RSV) group. Hematoxylin-eosin (HE) staining and tartrate-resistant acid phosphatase (TRAP) staining were used to observe the pathological changes in periodontal tissues. Immunohistochemical staining was used to detect nuclear factors κB receptor activator ligand (RANKL), osteoprotegerin (OPG), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and other osteogenic differentiation-related factors. The root resorption was analyzed by scanning electron microscope. The expression levels of SIRT1 and Nrf2 proteins in periodontal tissue were detected by Western blot. Results: Compared with the control group, the number of TRAP-positive cells in the periodontal tissue of the model group increased; the expression of RANKL was increased, and the expressions of OPG, RUNX2, and OCN were decreased; the root resorption rate was increased; the protein levels of Sirt1 and Nrf2 were decreased (P<0.05). Compared with the model group, the number of TRAP-positive osteoclasts in the periodontal tissue of the L-RSV group and the H-RSV group decreased; the expression of RANKL was decreased, and the expressions of OPG, RUNX2, and OCN were increased; the root resorption rate was significantly decreased; Sirt1, Nrf2 protein levels increased (P<0.05). Conclusion: RSV can promote the healing of periodontal tissue after tooth transplantation in rats, and the promotion may be related to the activation of the Sirt1/Nrf2 signaling pathway.

Key words： Resveratrol Rat Tooth transplantation Periodontal tissue Sirt1/Nrf2 signaling pathway

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.11.06 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I11/1790

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