miR-106b介导RANKL/RANK/OPG通路抗骨质疏松症的实验研究

doi:10.3969/j.issn.1006-6233.2022.11.07

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Abstract Objective: To explore the anti-osteoporosis effect of mirco ribonucleic acid 106b (miR-106b) mediated activator receptor of nuclear factor κB ligand (RANKL)/receptor activator of nuclear factor κB (RANK)/osteoprotegerin (OPG) pathway.Methods: Sixty Wistar rats were ovariectomized to prepare the osteoporosis rat model, and another 10 rats were only removed the fat around the ovary as the sham operation group. The 51 rats successfully modeled were randomly divided into model group, miR-106b mimics-NC group, miR-106b mimics group, miR-106b inhibitor-NC group and miR-106b inhibitor group.All groups except the model group were transfected with miR-106b mimic negative control, miR-106b mimic, miR-106b inhibitor negative control and miR-106b inhibitor respectively, and 10 rats were removed only the fat near the ovary as the sham operation group.X-ray bone densitometry was used to measure the bone mineral density of the rat femur; hematoxylin-eosin (HE) staining was used to observe the histopathological changes of the rat right femur; real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and immunoblotting (WB) were used to detect miR-106b expression, RANKL, RANK, OPG messenger ribonucleic acid (mRNA) expression and protein expression of the rat right femur respectively; dual luciferase reporter gene assay was performed to verify the targeting relationship between miR-106b and RANKL.Results: Bone mineral density, miR-106b expression, OPG mRNA and protein expression were increased (P<0.05), RNAKL mRNA and protein expression were decreased (P<0.05) in the right femoral tissue of the miR-106b mimics group compared to the model group and miR-106b mimics-NC group.Compared with the model group and miR-106b inhibitor-NC group, BMD, miR-106b expression, OPG mRNA and protein expression in the right femoral tissue of the miR-106b inhibitor group were reduced (P<0.05) and RNAKL mRNA and protein expression were increased (P<0.05).In the sham-operated group, a large number of bone trabeculae were closely arranged and of uniform thickness; in the model group, miR-106b mimics-NC group and miR-106b inhibitor-NC group, the number of bone cells was reduced, the arrangement was disordered and the gap between bone trabeculae was large; in the miR-106b mimics group, the number of bone cells was increased, the arrangement was tight and the thickness of bone trabeculae was increased; in the miR-106b inhibitor group showed a lack of osteoblasts, disorganized arrangement and large trabecular gaps. The dual luciferase reporter gene assay confirmed that miR-106b targets and regulates RANKL.Conclusion: Up-regulation of miR-106b expression inhibited RANKL expression, promoted OPG expression, increased femoral bone mineral density and reduced histopathological damage to the right femur in osteoporotic rats.

Key words： microRNA 106b Osteoporosis Nuclear factor-κB receptor-activating factor Nuclear factor-κB receptor-activating factor receptor Osteoprotegerin

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	WANG Zhengjun
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WANG Zhengjun,et al. Experimental Study of miR-106b Mediated RANKL/RANK/OPG Pathway Against Osteoporosis[J]. HeBei Med, 2022, 28(11): 1795-1800.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.11.07 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I11/1795

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