LINC00852调节miR-671-5p/MYH9信号轴对非小细胞肺癌细胞增殖侵袭及迁移的影响

doi:10.3969/j.issn.1006-6233.2024.10.01

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Abstract Objective: To investigate the effects of long non-coding RNA 00852 (LINC00852) on the proliferation, invasion, and migration of non-small cell lung cancer (NSCLC) cells by regulating the microRNA-671-5p (miR-671-5p)/myosin heavy chain 9 (MYH9) signaling axis. Methods: The expression levels of LINC00852, miR-671-5p, and MYH9 mRNA were detected in NSCLC tissues and adjacent normal tissues using qRT-PCR. A549 cells were randomly divided into several groups: Control group, sh-NC group, sh-LINC00852 group, sh-LINC00852+anti-NC group, and sh-LINC00852+anti-miR-671-5p group. The expression levels of LINC00852, miR-671-5p, and MYH9 mRNA were detected. Cell proliferation, invasion, and migration were assessed by colony formation assay, Transwell assay, and wound healing assay, respectively. Western blot was used to detect the protein expression of PCNA, MYH9, and MMP-9. Dual-luciferase reporter assay was performed to verify the interaction between LINC00852 and miR-671-5p, and between miR-671-5p and MYH9. Results: The expression levels of LINC00852 and MYH9 mRNA were significantly higher in NSCLC tissues compared with adjacent normal tissues, while the expression of miR-671-5p was significantly lower. Knockdown of LINC00852 inhibited the proliferation, invasion, and migration of A549 cells, and downregulated the expression of MYH9, PCNA, and MMP-9, while upregulating the expression of miR-671-5p. Dual-luciferase reporter assay confirmed that LINC00852 could directly target miR-671-5p, and miR-671-5p could directly target MYH9. Conclusion: LINC00852 inhibits the proliferation, invasion, and migration of NSCLC cells by regulating the miR-671-5p/MYH9 signaling axis.

Key words： Long non-coding RNA 00852 microRNA-671-5p Myosin heavy chain 9 Non-small cell lung cancer Proliferation Invasion Migration

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.10.01 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I10/1585

[1] Ratajczak-wielgomas K,Kmiecik A,Dziegiel P.Role of periostin expression in non-small cell lung cancer: periostin silencing inhibits the migration and invasion of lung cancer cells via regulation of MMP-2 expression[J].Int Mol Sci,2022,23(3):1240-1263.
[2] Skribek M,Rounis K,Tsakonas G,et al.Complications following novel therapies for non-small cell lung cancer[J].Intern Med,2022,291(6):732-754.
[3] Tuo Z,Liang L,Zhou R.LINC00852 is associated with poor prognosis in non-small cell lung cancer patients and its inhibition suppresses cancer cell proliferation and chemoresistance via the hsa-miR-145-5p/KLF4 axis[J].Gene Med,2021,23(12):3384.
[4] Li S J,Wu Y X,Liang Y H,et al.LncRNA HANR aggravates the progression of non-small cell lung cancer via mediating miRNA-140-5p[J].Eur Rev Med Pharmacol Sci,2020,24(2):704-711.
[5] Yang B,Zhang B,Qi Q,et al.CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis[J].BMC Pulm Med,2022,22(1):443-455.
[6] Chen M,Sun L X,Yu L,et al.MYH9 is crucial for stem cell-like properties in non-small cell lung cancer by activating mTOR signaling[J].Cell Death Discov,2021,7(1):282-291.
[7] J Saller J,Boyle T A.Molecular pathology of lung cancer[J].Cold Spring Harb Perspect Med,2022,12(3):37812-37853.
[8] Chen S.LINC00852 regulates cell proliferation,invasion,migration and apoptosis in hepatocellular carcinoma via the miR-625/E2F1 axis[J].Cell Mol Bioeng,2021,15(2):207-217.
[9] Zhang H,Du Y,Xin P,et al.The LINC00852/miR-29a-3p/JARID2 axis regulates the proliferation and invasion of prostate cancer cell[J].BMC Cancer,2022,22(1):1269-1281.
[10] Li Q,Wang X,Jiang N,et al.Exosome-transmitted linc00852 associated with receptor tyrosine kinase AXL dysregulates the proliferation and invasion of osteosarcoma[J].Cancer Med,2020,9(17):6354-6366.
[11] Harrison E B,Porrello A,Bowman B M,et al.A circle RNA regulatory axis promotes lung squamous metastasis via CDR1-mediated regulation of golgi trafficking[J].Cancer Res,2020,80(22):4972-4985.
[12] 覃波,陆录,赵涛.circ_DONSON靶向miR-671-5p影响肺癌细胞放射敏感性的实验研究[J].河北医学,2022,28(1):6-13.
[13] Liu Q,Cheng C,Huang J,et al.MYH9: A key protein involved in tumor progression and virus-related diseases[J].Biomed Pharmacother,2024,171(1):116118-116130.
[14] Liu J H,Yang H L,Deng S T,et al.The small molecule chemical compound cinobufotalin attenuates resistance to DDP by inducing ENKUR expression to suppress MYH9-mediated c-Myc deubiquitination in lung adenocarcinoma[J].Acta Pharmacol Sin,2022,43(10):2687-2695.
[15] 刘芳,彭岚竹,席菁乐.高表达MYH9通过激活AKT/c-Myc通路抑制非小细胞肺癌细胞凋亡[J].南方医科大学学报,2023,43(4):527-536.