2型糖尿病来源外泌体转运circ_001895对高糖胁迫下内皮祖细胞功能的影响

doi:10.3969/j.issn.1006-6233.2024.10.02

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Abstract Objective: To investigate the effects of exosomal circ_001895 transportation on endothelial progenitor cells under high glucose stress derived from patients with type 2 diabetes (T2DM). Methods: Serum samples were obtained from T2DM patients and divided into serum group and EXO group. The expression levels of circ_001895 in serum and EXO were detected by using RT-qPCR technology. EXOs derived from T2DM patients' serum with significantly elevated circ_001895 expression were named EXO-circ. Endothelial progenitor cells provided by Wuhan Procell were cultured and divided into the following three groups. Control Group: Cultured conventionally for 24 hours without additional treatment. High Glucose (HG) Group: Treated with 30 mmoL/L glucose for 24 hours. HG+EXO-circ Group: Treated with 30 mmoL/L glucose combined with 100μg/mL EXO-circ for 24 hours. The expression of circ_001895 in each group of cells was detected using RT-qPCR technology. Cell proliferation vitality was detected using the CCK-8 method. Cell apoptosis was detected using flow cytometry. The expression of angiogenic markers E-cadherin, N-cadherin, and Vimentin was detected using Western blot technology. Cell migration capacity was detected using the Transwell cell migration assay. Results: Compared to the serum group, the relative expression level of circ_001895 in the EXO group was significantly upregulated (P<0.05).Compared to the control group, the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG group were significantly upregulated (all P<0.05), while cell proliferation vitality, migration capacity, and the relative expression levels of N-cadherin and Vimentin were significantly downregulated (all P<0.05). Compared to the HG group, the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG+EXO-circ group were significantly upregulated (all P<0.05), while cell proliferation vitality, migration capacity, and the relative expression levels of N-cadherin and Vimentin were significantly downregulated (all P<0.05). Conclusion: Transportation of circ_001895 in exosomes from T2DM inhibits proliferation, migration, and vascular generation of endothelial progenitor cells under high glucose stress, while promoting apoptosis.

Key words： Type 2 diabetes Exosomes circ_001895 Endothelial progenitor cells Migration Vascular generation

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	ZHANG Jie
	ZHANG Li
	WANG Chenfei
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ZHANG Jie,ZHANG Li,WANG Chenfei, et al. Effect of Exosomal Transport of circ_001895 from Type 2 Diabetes Mellitus on Endothelial Progenitor Cells under High Glucose Stress[J]. HeBei Med, 2024, 30(10): 1592-1596.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.10.02 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I10/1592

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