龙血竭提取物调控HIF-1α/VEGF信号通路促进肛周脓肿大鼠创面愈合

doi:10.3969/j.issn.1006-6233.2024.12.04

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Abstract Objective: To analyze the effect of resina draconis extract on promoting wound healing in rats with perianal abscess by regulating HIF-1α/VEGF signaling pathway.Methods: Forty SPF grade SD rats were selected, with 10 as the control group and 30 used to construct the perianal abscess model. A total of 24 rats were successfully modeled and divided into a model group (8), a resina draconis extract group (8), and a resina draconis extract+HIF-1α inhibitor group (8). The control group and the model group were not treated. The resina draconis extract group was treated with resina draconis extract coated on sterile gauze and covered on the wound surface, while the resina draconis extract+HIF-1α inhibitor group was treated with resina draconis extract applied on the wound surface and injected with 10 μmoL/L HIF-1α inhibitor. After 14 days of administration, the pathological histology, wound healing, number of newly formed capillaries, fibroblast count, angiogenesis related factors, inflammatory factors, HIF-1α/VEGF pathway related mRNA expression levels, and HIF-1α/VEGF expression levels were observed in each group of rats.Results: Compared with the model group, the resina draconis extract group and the dragon's blood extract+HIF-1α inhibitor group had higher wound healing rates after 4 days, 9 days, and 14 days of treatment, and the resina draconis extract group had a higher wound healing rate (P<0.05). Compared with the control group, the number of newly formed capillaries and fibroblasts in the model group decreased, while the levels of bFGF, EGF, HIF-1α, VEGF related mRNA expression, HIF-1α, and VEGF expression decreased. The levels of TNF - α, IL-6, and IL - β increased. Compared with the model group, the resina draconis extract group and the resina draconis extract+HIF-1α inhibitor group increased the number of newly formed capillaries and fibroblasts, while the levels of bFGF, EGF, HIF-1α, VEGF related mRNA expression, HIF-1α, and VEGF increased. The levels of TNF - α, IL-6, and IL - β decreased, and the number of newly formed capillaries and fibroblasts in the resina draconis extract group was higher than that in the resina draconis extract+HIF-1α inhibitor group. The levels of EGF, HIF-1α, VEGF related mRNA expression, HIF-1α, and VEGF expression were higher in the resina draconis extract+HIF-1α inhibitor group than in the resina draconis extract+HIF-1α inhibitor group, the levels of TNF-α, IL-6, and IL - β were lower in the resina draconis extract+HIF-1α inhibitor group (P<0.05).Conclusion: The intervention of resina draconis extract on perianal abscess rats can promote wound healing by regulating the HIF-1α/VEGF signaling pathway, and has a good therapeutic effect.

Key words： Perianal abscess Resina draconis extract Hypoxia-inducible factor-1α / vascular endothelial growth factor Wound healing

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.12.04 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I12/1958

[1] Sarofim M,Ooi K.Reviewing perianal abscess management and recurrence:lessons from a trainee perspective[J].ANZ Surg,2022,92(7-8):1781-1783.
[2] Guner Ozenen G,Akaslan Kara A,Ozer A,et al.Perianal abscess in children:an evaluation of microbiological etiology and the effectiveness of antibiotics[J].Pediatr Surg Int,2023,39(1):272.
[3] 张馨,王莉芳,陈佳琪,等.龙血竭酚类提取物主要成分在Caco-2细胞中吸收机制研究[J].中国中药杂志,2020,45(20):4889-4895.
[4] 于泽洋,李天博,王江宁.复方芪参提取物对糖尿病足溃疡模型大鼠创面愈合及HIF-1α/VEGF/VEGFR2通路的影响[J].河北医学,2021,27(3):374-379.
[5] Tabari A,Kaplan JL,Huh SY,Moran CJ,Gee MS.Clinical characteristics and MRI-based phenotypes of perianal abscess formation in children with fistulizing crohn's disease[J].Front Pediatr,2022,10(1):10-13.
[6] 李振宇,刘建乔,芦煜,等.清化肛宁汤联合置管冲洗引流对肛周脓肿患者创面愈合、创面分泌物及氧化应激的影响[J].中国临床医生杂志,2024,52(6):741-744.
[7] 梁显南,颜帅.负压封闭引流联合龙血竭粉在水烫伤足踝创面修复中的应用[J].中国美容医学,2023,32(1):63-66.
[8] 吴超,王彩星,韩瑜娇.连翘苷对肛周脓肿大鼠创面血管新生的促进作用[J].中国皮肤性病学杂志,2020,34(12):1435-1442.
[9] 蒋宇,许建勇,时艳,等.复方黄柏液涂剂联合消疮合剂对肛周脓肿术后创面愈合、临床症状及炎症反应的影响[J].国际中医中药杂志,2023,45(8):968-972.
[10] 李洪剑,修洁,谭思思,等.散结透脓方外敷联合消肿清热解毒汤内服治疗泛发性肛周脓肿术后的效果及对创面愈合时间、肛门功能的影响[J].河北中医,2024,46(5):740-744.
[11] 胡建文,冯群虎,王业皇,等.王氏生肌散对肛周脓肿大鼠创面愈合及HIF-1α/VEGF信号通路的影响[J].陕西中医,2023,44(9):1179-1183.
[12] 戚新春,易建华.长链非编码RNA BCYRN1对脑胶质瘤大鼠HIF-1α/VEGF信号通路和血管生成的作用机制[J].陕西医学杂志,2023,52(4):376-379.