黄芩苷调节TGF-β<sub>1</sub>/Smad信号通路对重症肺炎大鼠肺组织炎症反应的影响

doi:10.3969/j.issn.1006-6233.2024.12.05

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1614 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the effect of baicalin (BC) on the inflammatory response of lung tissue and the transforming growth factor-β₁ (TGF-β₁)/Smad signaling pathway in severe pneumonia (SP) rats.Methods: SD rats were randomly separated into a control group, a model group (SP group), low, medium, and high-dose baicalin groups (BC-L, BC-M, BC-H groups), and a high-dose baicalin+pathway activator group (BC-H+SRI-011381 group), with 18 rats in each group. Except for the control group, all other rats were used to construct an SP rat model. The lung coefficient (LI) and wet/dry weight ratio (W/D) of rats in each group were measured. ELISA was applied to detect the levels of inflammatory factors and severe pneumonia related biomarkers procalcitonin (PCT) and C-reactive protein (CRP) in alveolar lavage fluid. Wright's staining was applied to detect the number of neutrophils (NEU), eosinophils (EOS), and lymphocytes (LYM) in the sediment of alveolar lavage fluid. HE staining was applied to observe the pathological morphology of lung tissue. Western blot was applied to detect the expression of TGF-β₁/Smad signaling pathway related proteins.Results: Compared with control group, SP group showed damage in lung tissue structure, with some areas of alveolar edema showing bleeding, thickening of alveolar walls and lung interstitium, and infiltration of a large number of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β₁, p-Smad2/Smad2, and p-Smad3/Smad3 were elevated (P<0.05). Compared with SP group, BC-L, BC-M, and BC-H groups showed improvement in lung tissue structure damage, with relatively normal alveolar morphology, reduced edema, and reduced infiltration of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β₁, p-Smad2/Smad2, and p-Smad3/Smad3 were reduced (P<0.05). Compared with BC-H group, BC-H+SRI-011381 group showed more severe damage to lung tissue structure and alveolar edema, with increased infiltration of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β₁, p-Smad2/Smad2, and p-Smad3/Smad3 were elevated (P<0.05).Conclusion: Baicalin can improve lung tissue inflammation in SP rats, and its mechanism of action is related to the inhibition of TGF-β₁/Smad pathway.

Key words： Baicalin TGF-β₁/Smad signaling pathway Severe pneumonia

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	YANG Zongyu
	CAI Wei
	XIA Chen
	et al

Cite this article:

YANG Zongyu,CAI Wei,XIA Chen, et al. Effect of Baicalin on the Inflammatory Response of Lung Tissue in Severe Pneumonia Rats by Regulating the TGF-β₁/Smad Signaling Pathway[J]. HeBei Med, 2024, 30(12): 1965-1971.

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.12.05 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I12/1965

[1] Garg M,Johri S,Sagar S,et al.Cardiolipin-mediated PPARγ S112 phosphorylation impairs IL-10 production and inflammation resolution during bacterial pneumonia[J].Cell Rep,2021,34(6):108736-108773.
[2] Zhang P,Shi Q,Hu H,et al.Emergence of ceftazidime/avibactam resistance in carbapenem-resistant Klebsiella pneumoniae in China[J].Clin Microbiol Infect,2020,26(1):124-131.
[3] 吴文娟,曾妮,王硕莹,等.黄芩苷对支原体肺炎小鼠TLR4/NF-κB信号通路的抗炎及肺功能保护作用[J].中华医院感染学杂志,2023,33(23):3521-3526.
[4] Li L,Guan J,Lin R,et al.Astragaloside IV alleviates lung inflammation in Klebsiella pneumonia rats by suppressing TGF-β₁/Smad pathway[J].Braz Med Biol Res,2023,56(1):12203-12212.
[5] 邝哲姝,吴福玲.黄芩苷在呼吸系统疾病中的研究进展[J].国际医药卫生导报,2023,29(13):1794-1797.
[6] Li L,Cui H,Zhang Y,et al.Baicalin ameliorates multidrug-resistant pseudomonas aeruginosa induced pulmonary inflammation in rat via arginine biosynthesis[J].Biomed Pharmacother,2023,162(1):114660-114672.
[7] He S,Peng H,Wu M,et al.Combined exposure to multiwalled carbon nanotubes and dibutyl phthalates aggravated airway inflammation in rats[J].Food Chem Toxicol,2023,182(1):114188-114198.
[8] Ma J,Li L,Qie X,et al.Value of combined detection of PCT,CRP,and FIB in differentiating viral infection from bacterial infection in severe pneumonia[J].Clin Lab,2023,69(11):1-10.
[9] 陈志刚.电子支气管镜肺泡灌洗术对老年重症肺炎并发呼吸衰竭患者临床肺部感染评分、肺功能及炎症指标的影响[J].中外医学研究,2024,22(12):140-145.
[10] 王田田,邓扬嘉,孙小平.瓜蒌大黄汤治疗重症肺炎随机对照研究[J].中国中西医结合杂志,2024,44(4):407-413.
[11] Zhou W,Chen J,Xi Z,et al.Characteristics of lung microbiota in children's refractory mycoplasma pneumoniae pneumonia coinfected with human adenovirus B[J].Can Infect Dis Med Microbiol,2022,2022(1):7065890-7065897.