组织miR-219 miR-34a DCST1-AS1与非小细胞肺癌临床病理特征相关性及诊断价值

doi:10.3969/j.issn.1006-6233.2025.02.020

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Abstract Objective: To explore the correlation between tissue microRNA-219 (miR-219), microRNA-34a (miR-34a), and DC-STAMP domain containing 1-antisense 1 (DCST1-AS1) with the clinicopathological features in non-small cell lung cancer (NSCLC), as well as their diagnostic value. Methods: A total of 120 NSCLC patients admitted to the Nuclear Industry 416 Hospital from May 2022 to May 2024 were enrolled. The cancerous tissues and adjacent tissues were taken, and the expression of miR-219, miR-34a, and DCST1-AS1 in the cancerous tissues and adjacent tissues was determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The expression of miR-219, miR-34a, and DCST1-AS1 in patients with different clinicopathological features was compared. Spearman correlation coefficient analysis was used to analyze the correlation between the expression of miR-219, miR-34a, and DCST1-AS1 in tissues and clinicopathological features. Receiver operating characteristic (ROC) curve, area under the curve (AUC), and decision curve analysis (DCA) were used to analyze the diagnostic value and clinical net benefit of miR-219, miR-34a, and DCST1-AS1 in NSCLC. Results: The expression of miR-219 (0.68±0.20 vs 1.00±0.30) in the cancerous tissue of the study subjects was significantly lower than that in the adjacent tissue, while the expressions of miR-34a (1.31±0.39 vs 1.00±0.28) and DCST1-AS1 (1.39±0.42 vs 1.05±0.31) were significantly higher than those in the adjacent tissue (t=9.722, 7.073, 7.135, P<0.05). The tumor, node, metastasis (TNM) staging,tumor differentiation, and lymph node metastasis were closely associated with the expression of tissue miR-219 (r = -0.644, 0.650, -0.601), miR-34a (r = 0.613, -0.604, 0.627), and DCST1-AS1 (r = 0.605, -0.620, 0.616) (P<0.05). The AUC of the combined diagnosis of miR-219, miR-34a, and DCST1-AS1 for NSCLC was 0.934 (0.895-0.962), with the highest clinical net benefit rate within the range of 0.2~1.0. Conclusion: Tissue miR-219, miR-34a, and DCST1-AS1 expression are closely associated with clinicopathological features of NSCLC patients. The combination of the three can be used as a reliable scheme for the diagnosis of NSCLC, providing an objective evidence for precise diagnosis and treatment.

Key words： Non-small cell lung cancer Clinicopathological features Micro ribonucleotide-219 Micro ribonucleotide-34a DC-STAMP domain containing 1-antisense 1

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.02.020 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I2/291

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