基于miR-146a/TRAF6轴探讨LncRNA NORAD对非霍奇金淋巴瘤细胞焦亡增殖和侵袭的影响

doi:10.3969/j.issn.1006-6233.2025.07.06

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Abstract Objective: To investigate the effect of lncRNA NORAD on pyroptosis, proliferation and invasion of non-Hodgkin's lymphoma cells based on miR-146a/TRAF6 axis. Methods: NHL cell lines Jurkat, Raji and normal B cells were cultured. The expression levels of lncRNA NORAD, miR-146a and TRAF6 were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Raji cell line was selected for lncRNA NORAD knockdown treatment (NORAD-siNC group, NORAD-si group). Western blot was used to detect the expression levels of NOD-like receptor protein 3 (NLRP3), Cleaved caspase1, Gasdermins-DN terminal domain (N-GSDMD) and TRAF6. qRT-PCR was used to detect the expression level of miR-146a. The levels of interleukin-1β (IL-1β) and IL-18 in cell culture supernatant were detected by kits. EDU assay was used to detect cell proliferation activity, and Transwell assay was used to detect cell invasion activity. Then, the correlation between lncRNA NORAD and miR-146a, and between miR-146a and TRAF6 was verified by dual-luciferase activity detection. Results: Compared with normal B cells, the expression of lncRNA NORAD and TRAF6 was increased in Jurkat and Raji cells, while the expression of miR-146a was decreased. Compared with cells in the NORAD-siNC group, cells in the NORAD-si group had increased expression levels of miR-146a, NLRP3, N-GSDMD and Cleaved caspase1, decreased expression level of TRAF6, weakened cell proliferation and invasion activities, and increased levels of IL-1β and IL-18 in cell culture supernatant. The results of dual-luciferase assay showed that there were target binding relationships between lncRNA NORAD and miR-146a, and between miR-146a and TRAF6. Conclusion: lncRNA NORAD is up-regulated in NHL cells. Knocking down the expression of lncRNA NORAD can induce pyroptosis of NHL cells and inhibit cell proliferation and invasion through the miR-146a/TRAF6 axis.

Key words： Long non-coding RNA NORAD Non-Hodgkin lymphoma Pyroptosis MicroRNA 146a Tumor necrosis factor receptor-associated protein 6

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.07.06 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I7/1087

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