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| The Effect of Wenyang Tongpi Xiaoji Prescription on the Apoptosis of Liver Cancer Mouse Cells by Regulating the Mitochondrial Apoptosis Pathway |
| YANG Chun, XIANG Caiqiong, QIN Xin, et al |
| Jingzhou Traditional Chinese Medicine Hospital, Hubei Jingzhou 434000, China |
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Abstract Objective: To investigate whether the Wenyang Tongpi Xiaoji prescription (WYTP) can promote apoptosis of liver cancer mouse cells by regulating the mitochondrial apoptosis pathway. Methods: H22 liver cancer cells were cultured, and a nude mouse model of H22 liver cancer transplantation was established. The nude mice were assigned to a control group, a positive drug group, a low-dose WYTP (WYTP-L) group, a medium-dose WYTP (WYTP-M) group, and a high-dose WYTP (WYTP-H) group. Tumor growth was observed, and tumor inhibition rate was calculated. HE staining was used to observe the morphology of tumor tissues. Mitochondrial membrane potential (MMP) was analyzed by mitochondrial red fluorescent probe. TUNEL staining was performed to detect cell apoptosis in tumor tissues. Western blot was used to detect the protein expression levels of Cleaved-Caspase-9, Caspase-9, Caspase-3, Cleaved-Caspase-3, Cyt C, Bax, Bcl-2, and PCNA. Results: The survival status of nude mice in each group was basically good. Compared with the control group, the tumor cells of nude mice in the WYTP-L group, WYTP-M group, WYTP-H group, and positive drug group showed loose arrangement, varying degrees of nuclear condensation and vacuolization, and cell necrosis, the tumor mass, tumor volume, mitochondrial membrane potential, the protein expression levels of Bcl-2 and PCNA decreased, the tumor inhibition rate, number of apoptotic tumor cells, and expression levels of Cleaved-Caspase-9, Caspase-9, Caspase-3, Cleaved-Caspase-3, Cyt C, and Bax proteins in tumor tissue cells increased (P<0.05). There was no statistically prominent difference in the above indicators between the positive drug group and the WYTP-H group (P>0.05). Conclusion: WYTP can promote apoptosis of liver cancer mouse cells and inhibit cell proliferation by regulating the mitochondrial apoptosis pathway.
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2025, Vol. 31 
