miR-106b-5p在结肠癌组织中的表达及其对人结肠癌LOVO/HCT116细胞生物学功能的影响

doi:10.3969/j.issn.1006-6233.2025.08.07

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Abstract Objective: To explore the expression of miR-106b-5p in colon cancer tissues and its influences on biological function of human colon cancer LOVO/HCT116 cells. Methods: The expression of has-miR-106b-5p in human colon cancer tissues and para-carcinoma tissues was detected by real-time fluorescent quantitative PCR (RT-PCR). Taking colon cancer LOVO/HCT116 cells as the study background, cell lines with miR-106b-5p overexpression and their negative controls (miR-106b-5p mimic group, mimic NC group) were constructed by miR-106b-5p-mimic and mimic NC, and they were verified by RT-PCR. The cells proliferation, cycle distribution, apoptosis, migration and invasion after transfection were detected by CCK8, flow cytometry and Tanswell assay, respectively. The expressions of related proteins were detected by Western blot. Results: The expression of has-miR-106b-5p in human colon cancer tissues was lower than that in para-carcinoma tissues (t=101.720, P<0.001). After transfection with miR-106b-5p, expression of has miR-106b-5p in LOVO/HCT116 cells in miR-106b-5p mimic group was higher than that in mimic-NC group (P<0.05). Compared with mimic group, cell proliferation rates were decreased in miR-106b-5p mimic group at 24, 48, 72, 96 and 120h after transfection (P<0.05), proportion of G0/G1 stage and apoptosis rate were increased, proportion of S stage and number of cells migration and invasion cells were decreased (P<0.05), expressions of vimentin (Vimentin), phosphorylated extracellular regulated kinase 1/2 (p-ERK1/2) and phosphorylated protein kinase B1/2/3 (p-AKT1/2/3) proteins were decreased (P<0.05), and expressions of E-cadherin and cleaved cysteine proteinase 3 (cleaved-caspase3) proteins were increased (P<0.05). Conclusion: The expression of miR-106b-5p is down-regulated in colon cancer tissues. Overexpression of miR-106b-5p can inhibit proliferation, migration and invasion of human colon cancer LOVO/HCT116 cells, and promote apoptosis, which may be related to the regulation of AKT and ERK signaling pathways.

Key words： Colon cancer miR-106b-5p Proliferation Apoptosis Migration Invasion

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	Articles by authors
	MA Hailin
	JIANG Sheng
	MA Zhiping

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MA Hailin,JIANG Sheng,MA Zhiping. Expression of miR-106b-5p in Colon Cancer Tissues and its Influences on Biological Function of Human Colon Cancer LOVO/HCT116 Cells[J]. HeBei Med, 2025, 31(8): 1269-1275.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.08.07 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I8/1269

[1] Abedizadeh R,Majidi F,Khorasani HR,et al.Colorectal cancer:a comprehensive review of carcinogenesis,diagnosis,and novel strategies for classified treatments[J].Cancer Metastasis Rev,2024,43(2):729-753.
[2] Zuo H,Liu S,Li X,et al.miR-23a-3p promotes the development of colon cancer by inhibiting the expression of NDRG4[J].Clin Transl Oncol,2023,25(4):933-940.
[3] Huang Z,Kaller M,Hermeking H.CRISPR/Cas9-mediated inactivation of miR-34a and miR-34b/c in HCT116 colorectal cancer cells:comprehensive characterization after exposure to 5-FU reveals EMT and autophagy as key processes regulated by miR-34[J].Cell Death Differ,2023,30(8):2017-2034.
[4] 王玉宁,宋聚星,田志刚,等.白皮杉醇调控miR-106b-5p/RUNX3轴对宫颈癌细胞迁移及侵袭的影响[J].天津医药,2023,51(8):814-819.
[5] 温一阳,徐成阳,胡晓舒.长链非编码RNA GAS5靶向微小RNA-106b-5p对胃癌细胞增殖、迁移和侵袭的影响[J].中华实验外科杂志,2024,41(2):243-246.
[6] 方洪生,骆洋,林海萍,等.miR-106b-5p靶向调控细胞周期蛋白D1抑制结直肠癌细胞增殖、迁移与侵袭[J].现代肿瘤医学,2022,30(6):949-955.
[7] 孙丽丽,白冰,杨霞,等.贝母素乙通过诱导G0/G1期阻滞抑制结肠癌细胞的增殖[J].中国肿瘤生物治疗杂志,2024,31(4):326-332.
[8] Wang W,Jin J,Zhou Z,et al.Snail inhibits metastasis via regulation of E-cadherin and is associated with prognosis in colorectal cancer[J].Oncol Lett,2023,25(6):271-280.
[9] Zhang K,Dong Y,Li M,et al.Clostridium butyricum inhibits epithelial-mesenchymal transition of intestinal carcinogenesis through downregulating METTL3[J].Cancer Sci,2023,114(8):3114-3127.
[10] 赵建刚,赵光远,鲍双振,等.LncRNA SNHG16通过miR-106b-5p/PHF1轴调节结直肠癌细胞的增殖、凋亡、迁移和侵袭[J].中国比较医学杂志,2023,33(2):56-65.
[11] 黄雪茹,丁绪浩,陈素贤,等.PMS2通过ERK/ERCC1通路对结肠癌SW 480细胞生物学行为的影响[J].吉林大学学报(医学版),2023,49(4):931-940.
[12] 王双双,黄晓伟,李淼,等.藤龙补中汤对大肠癌转移和整合素-FAK-ERK1/2通路蛋白表达的影响[J].中华中医药杂志,2020,35(4):1697-1699.
[13] Saiprasad G,Chitra P,Manikandan R,et al.Hesperetin regulates PI3K/Akt and mTOR pathways to exhibit its antiproliferative effect against colon cancer cells[J].Biotech Histochem,2024,99(6):287-304.
[14] 严涵,郭绍兰,戴学敏,等.红花苷联合miR-214对肺癌细胞增殖及凋亡的作用[J].河北医学,2024,30(12):1937-1945.
[15] 何振涛,吴昊,代德福,等.SPP1通过AKT/GSK3β信号通路调控结直肠癌细胞的增殖、迁移与侵袭能力[J].肿瘤,2024,44(4):358-368.