利多卡因抑制RhoA/ROCK通路对食管癌细胞增殖侵袭血管生成拟态的影响

doi:10.3969/j.issn.1006-6233.2025.09.07

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Abstract Objective: To explore the effects of lidocaine on the proliferation, invasion, and vascular mimicry of esophageal cancer (EC) cells by inhibiting the small GTPase (RhoA)/Rho-associated coiled-coil containing protein kinase (ROCK) pathway. Methods: The human EC cell line Eca-109 was cultured in vitro and assigned into NC group, low-dose lidocaine group (lidocaine 5 mmol/L), medium dose lidocaine group (lidocaine 10mmol/L), high-dose lidocaine group (lidocaine 15 mmol/L), and high-dose lidocaine+RhoA/ROCK pathway activator (LPA) group (lidocaine 15 mmol/L+50μmol/L LPA). The clone formation experiment, Transwell chamber experiment, and Matrigel lumen formation experiment were used to detect the proliferation and invasion abilities, and angiogenesis of Eca-109 cells in each group. The qRT-PCR experiment was used to detect the RhoA and ROCK mRNA of Eca-109 cells in each group. Western blot was used to detect the RhoA, ROCK, CCND1, Myc, VEGF, and TIMP-1 proteins of Eca-109 cells. Results: Compared with the NC group, the low, medium, and high dose lidocaine groups showed a decrease in the numbers of Eca-109 cell colony formation, invasion, and angiogenesis tubular structures, reduced RhoA, ROCK mRNA, and their proteins, and prominently decreased CCND1, Myc, and VEGF proteins, and prominently increased TIMP-1 protein (P<0.05). Compared with the high-dose lidocaine group, the high-dose lidocaine+LPA group showed an increase in the numbers of Eca-109 cell colony formation, invasion, and angiogenesis tubular structures, increased RhoA, ROCK mRNA, and their proteins, and prominently increased CCND1, Myc, and VEGF proteins, and prominently reduced TIMP-1 protein (P<0.05). Conclusion: Lidocaine can inhibit proliferation, invasion, and vasculogenic mimicry of EC cells by suppressing RhoA/ROCK pathway.

Key words： Esophageal cancer Lidocaine RhoA/ROCK pathway Proliferation Invasion Vasculogenic mimicry

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	NITeweibuzi
	CHEN Yong
	ZHANG Yufeng
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NITeweibuzi,CHEN Yong,ZHANG Yufeng, et al. Effects of Lidocaine on the Proliferation Invasion and Vascular Mimicry of Esophageal Cancer Cells by Inhibiting the RhoA/ROCK Pathway[J]. HeBei Med, 2025, 31(9): 1446-1452.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.09.07 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I9/1446

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