LncRNA SNHG7靶向调控miR-146b-5p对前列腺癌细胞迁移及侵袭的影响

doi:10.3969/j.issn.1006-6233.2025.09.08

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Abstract Objective: To investigate the effects of long non-coding RNA (LncRNA) small nucleolar RNA host gene 7 (SNHG7) on migration and invasion of prostate cancer (PCa) cells through targeted regulation of microRNA-146b-5p (miR-146b-5p). Methods: QRT-PCR was used to measure the expression of LncRNA SNHG7 and miR-146b-5p in cancer tissues and adjacent tissues of 42 PCa patients who underwent PCa surgery in our hospital from August 2023 to April 2024. The logarithmic phase DU145 cells were grouped into normal group, NC-shRNA group, SNHG7-shRNA group, SNHG7-shRNA+anti-NC group, and SNHG7-shRNA+anti-miR-146b-5p group. MTT assay, scratch assay, and Transwell assay were used to detect the proliferation, migration, and invasion of DU145 cells, respectively. In addition, Western blot was used to detect the expression of epithelial mesenchymal transition (E-cadherin, Vimentin) proteins in DU145 cells. Results: The expression of LncRNA SNHG7 in PCa tissue was higher than that in adjacent tissues (t=30.622, P<0.001), and the expression of miR-146b-5p was lower than that in adjacent tissues (t=26.869, P<0.001). The A490 value, scratch healing rate, invasion number, LncRNA SNHG7, and Vimentin expression in SNHG7-shRNA group were lower than those in NC-shRNA group and Control group, while miR-146b-5p and E-cadherin were higher than those in NC-shRNA group and Control group (P<0.05). The A490 value, scratch healing rate, invasion number, LncRNA SNHG7, and Vimentin expression in SNHG7-shRNA+anti-miR-146b-5p group were higher than those in SNHG7-shRNA group and SNHG7-shRNA+anti-NC group, while miR-146b-5p and E-cadherin were lower (P<0.05). LncRNA SNHG7 could target negative regulation of miR-146b-5p. Conclusion: Silencing LncRNA SNHG7 can inhibit the migration and invasion of PCa cells, and it may be achieved by targeting negative regulation of miR-146b-5p.

Key words： Long non-coding Small nucleolar RNA host gene 7 MicroRNA-146b-5p Prostate cancer Migration Invasion

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.09.08 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I9/1452

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