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2024 Vol. 30, No. 12 Published: 2024-12-31 1937 Synergic Effects of Carthamin and miR-214 on Proliferation and Apoptosis of Lung Cancer Cells YAN Han, et al DOI: 10.3969/j.issn.1006-6233.2024.12.01 Objective: To investigate the effects of carthamin combined with miR-214 on the proliferation and apoptosis of lung cancer cells.Methods: Quantitative real-time PCR was conducted to determine miR-214 expression in human normal lung epithelial cells BEAS-2B and lung cancer cells A549 and H1299. miR-214 mimic (miR-214) and negative control (miR-NC) were transfected into A549 and H1299 cells. A549 and H1299 cells were divided into control, carthamin, miR-214 and carthamin +miR-214 groups. Cells in carthamin group were treated with 80μmoL/L carthamin, cells in miR-214 group were transfected with miR-214, and cells in carthamin+miR-214 group were co-treated with carthamin and miR-214. The cells without any treatment were used as control group. CCK-8, EdU and flow cytometry assays were performed to evaluate cell viability, proliferation, cell cycle and apoptosis, respectively. The protein expressions were analyzed with Western blot. A xenograft tumor model was used to evaluate the synergy of carthamin and miR-214 on lung cancer growth in vivo.Results: Compared with BEAS-2B cells, miR-214 expression was significantly reduced in A549 and H1299 cells. miR-214-transfected A549 and H1299 cells expressed higher level of miR-214, compared to miR-NC. Compared with control group, cell viability, EdU-positive cells and S-phase cells were markedly reduced, while G0/G1-phase cells and apoptotic cells were substantially increased in carthamin and miR-214 groups; the cell viability, EdU-positive cells and S-phase cells were less, while the G0/G1-phase cells and apoptotic cells were more in carthamin+miR-214 group than those in carthamin and miR-214 groups. Compared with control group, the phosphorylation of Akt and PI3K and protein expressions of Bcl-2, Cyclin D1 and CDK4 were considerably downregulated, while the expressions of Bax and cl-Caspase-3 were significantly up-regulated in carthamin and miR-214 groups. Akt and PI3K phosphorylations and protein levels of Bcl-2, Cyclin D1 and CDK4 in carthamin +miR-214 group were much lower, while Bax and cl-Caspase-3 expressions were higher than those in carthamin and miR-214 groups. In vivo experiments showed that compared with control group, the tumor volume and weight of mice were significantly reduced in carthamin and miR-214 groups, which were further smaller in carthamin+miR-214 group than those in carthamin and miR-214 groups.Conclusion: Carthamin combined with miR-214 could inhibit the proliferation but promote apoptosis of lung cancer cells in vitro and in vivo, probably through the inactivation of PI3K/Akt signaling pathway. 2024 Vol. 30 (12): 1937-1944 [Abstract] ( 76 ) HTML (1 KB)  PDF (3007 KB)  ( 68 ) 1945 Study on the Regulation of Growth and Metastasis of Esophageal Squamous Cell Carcinoma Cells by LncRNA UCA1 TANG Chengqiong, Baerxiaguli Zabihula, LU Yanrong DOI: 10.3969/j.issn.1006-6233.2024.12.02 Objective: To investigate the effects of LncRNA UCA1 on the in vivo and in vitro growth and metastasis of esophageal squamous cell carcinoma cells.Methods: qRT-PCR was used to detect the expression of LncRNA UCA1 in human esophageal epithelial cells (HEEC), human esophageal squamous cell carcinoma cells (EC109, EC9706, KYSE150, and KYSE450). EC109 and EC9706 cells were divided into si-NC group and si-UCA1 group. CCK8 and flow cytometry were used to detect cell proliferation and cell cycle, Transwell method was used to detect cell migration and invasion. Western blot was used to detect the relative expression levels of E-cadherin, Vimentin, and MMP-9 in cells. 1×106 EC109 cells of si-NC and si-UCA1 groups were inoculated into the left axilla of BALB/c-A nude mice, after 4 weeks of feeding, the tumor volume and weight of the mice were observed and recorded.Results: The expression of LncRNA UCA1 in EC109, EC9706, KYSE150, and KYSE450 was significantly higher than that in HEEC cells (P<0.001). Compared with the si-NC group, the proliferation, migration, and invasion abilities of EC109 and EC9706 cells in the si-UCA1 group were significantly reduced (P<0.05), cell cycle was significantly arrested (P<0.05), the expression of E-cadherin was significantly upregulated (P<0.001), and the expression of Vimentin and MMP-9 were significantly downregulated (P<0.001). The tumor volume and weight of mice in the si-UCA1 group were significantly lower than those in the si-NC group (P<0.001). Conclusion: Inhibiting LncRNA UCA1 can inhibit the proliferation, migration, and invasion ability of esophageal squamous cell carcinoma cells, as well as their growth in mice. 2024 Vol. 30 (12): 1945-1951 [Abstract] ( 40 ) HTML (1 KB)  PDF (1729 KB)  ( 15 ) 1951 Effect of LincRNA 00312 on the Malignant Biological Behavior of Ovarian Cancer Cells by Regulating SOSTDC1-Mediated BMP-Smads Axis NIU Lina, CHEN Zhuojing, XU Wangxin, et al DOI: 10.3969/j.issn.1006-6233.2024.12.03 Objective: To investigate the effect of long noncoding RNA (LncRNA) 00312 on the malignant biological behavior of ovarian cancer (OC) cells by regulating target sclerostin domain containing protein 1 (SOSTDC1)-mediated bone morphogenetic protein (BMP)-smads axis. Methods: The cancer tissues and adjacent tissues of 15 patients undergoing ovarian cancer radical operation in Yuncheng Central Hospital from September 2021 to December 2022 were collected, and human normal ovarian epithelial cells (IOSE80) and EC cells (SKOV3 and OVCAR) were selected as target cells. The expression of LincRNA 00312 in OC cancer and adjacent tissues, SKOV3 and OVCAR was analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). SKOV3 cells were divided into si-NC group, si-LincRNA 00312 group, si-LincRNA 00312 + si-SOSTDC1 group, OE-NC group and OE-SOSTDC1 group. Kyoto Encyclopedia of Genes and Genomes (KEGG) was performed to analyze the possible downstream pathways of LincRNA 00312. Western blot was used to detect the expression of SOSTDC1 and BMP-smad pathway-related proteins in OC tissues and SKOV3 cells in each group. Colony formation assay, scratch assay and Transwell assay were used to detect the proliferation, migration and invasion of SKOV3 cells in each group, respectively. The targeting effects of LincRNA 00312 and SOSTDC1 were detected by bioinformatics prediction, dual luciferase reporter gene and RNA-protein pull-down assay. Results: The expression of LincRNA 00312 in OC cancer tissues was significantly higher than that in adjacent tissues (t=7.288, P<0.05), which in SKOV3 and OVCAR3 cells was significantly increased than that in IOSE80 cells (t=27.805, 8.860, all P<0.05). KEGG pathway analysis showed that LincRNA 00312 was mainly related to BMP-smad signaling pathway, transcription disorders in tumors and mitogen-activated protein kinases (MAPK) signaling pathway. Bioinformatics analysis showed that there was a possible binding site between LincRNA 00312 and SOSTDC1 mRNA sequence. Compared with the adjacent tissues, the mRNA-protein expression levels of SOSTDC1 in cancer tissues were significantly decreased (t=23.653, 27.498, all P<0.05), and the protein expression levels of bone morphogenetic protein-2 (BMP2), BMP4, BMP7, amad1 domain-containing protein 1 (amad1) / 5 / 9 and p-smad1 / 5 / 9 were significantly increased (t=5.952, 7.322, 8.024, 7.094 and 5.512, all P<0.05). Compared with the si-NC group, the si-LincRNA 00312 group had significantly decreased colony numbers, wound healing rate, number of invasive cells (t=6.914, 4.729, 11.321, all P<0.05). The dual luciferase reporter gene found that the luciferase activity of pGL3-SOSTDC1-WT in SKOV3 cells in OE-SOSTDC1 group was significantly lower than that in OE-NC group (t=19.744, P<0.05). RNA-protein pull-down assay showed that the enrichment multiple of SOSTDC1 in transfected Bio-LincRNA 00312-WT cells was significantly higher than that in transfected Bio-LincRNA 00312-MUT cells (t=36.374, P<0.05). Compared with the OE-NC group, the OE-SOSTDC1 group had significantly increased expression of SOSTDC1 protein in SKOV3 cells ( t=39.491, P<0.05 ), and decreased expression of BMP2, BMP4, BMP7, amad1 / 5 / 9 and p-SAMD1 / 5 / 9 proteins (t=19.696, 19.752, 14.203, 45.928 and 21.637, all P<0.05). Compared with the si-LincRNA 00312 group, the si-LincRNA 00312 + si-SOSTDC1 group presented significantly increased colony numbers, migration rate, number of invasive cells and the expression of BMP2, BMP4, BMP7, smad1 / 5 / 9 and p-smad1 / 5 / 9 proteins (t=8.911, 8.193, 8.873, 14.203, 12.222, 20.821, 19.365 and 31.225, all P<0.05). Conclusion: The upregulated LincRNA 00312 in OC tissues can promote cancer cell proliferation, migration and invasion by regulating the SOSTDC1-mediated BMP-smad signaling pathway. 2024 Vol. 30 (12): 1951-1957 [Abstract] ( 33 ) HTML (1 KB)  PDF (2496 KB)  ( 14 ) 1958 Resina Draconis Extract Regulating HIF-1α/VEGF Signaling Pathway to Promote Wound Healing in Rats with Perianal Abscess WANG Shuai, GUO Jinling, ZHAO Xuefang, et al DOI: 10.3969/j.issn.1006-6233.2024.12.04 Objective: To analyze the effect of resina draconis extract on promoting wound healing in rats with perianal abscess by regulating HIF-1α/VEGF signaling pathway.Methods: Forty SPF grade SD rats were selected, with 10 as the control group and 30 used to construct the perianal abscess model. A total of 24 rats were successfully modeled and divided into a model group (8), a resina draconis extract group (8), and a resina draconis extract+HIF-1α inhibitor group (8). The control group and the model group were not treated. The resina draconis extract group was treated with resina draconis extract coated on sterile gauze and covered on the wound surface, while the resina draconis extract+HIF-1α inhibitor group was treated with resina draconis extract applied on the wound surface and injected with 10 μmoL/L HIF-1α inhibitor. After 14 days of administration, the pathological histology, wound healing, number of newly formed capillaries, fibroblast count, angiogenesis related factors, inflammatory factors, HIF-1α/VEGF pathway related mRNA expression levels, and HIF-1α/VEGF expression levels were observed in each group of rats.Results: Compared with the model group, the resina draconis extract group and the dragon's blood extract+HIF-1α inhibitor group had higher wound healing rates after 4 days, 9 days, and 14 days of treatment, and the resina draconis extract group had a higher wound healing rate (P<0.05). Compared with the control group, the number of newly formed capillaries and fibroblasts in the model group decreased, while the levels of bFGF, EGF, HIF-1α, VEGF related mRNA expression, HIF-1α, and VEGF expression decreased. The levels of TNF - α, IL-6, and IL - β increased. Compared with the model group, the resina draconis extract group and the resina draconis extract+HIF-1α inhibitor group increased the number of newly formed capillaries and fibroblasts, while the levels of bFGF, EGF, HIF-1α, VEGF related mRNA expression, HIF-1α, and VEGF increased. The levels of TNF - α, IL-6, and IL - β decreased, and the number of newly formed capillaries and fibroblasts in the resina draconis extract group was higher than that in the resina draconis extract+HIF-1α inhibitor group. The levels of EGF, HIF-1α, VEGF related mRNA expression, HIF-1α, and VEGF expression were higher in the resina draconis extract+HIF-1α inhibitor group than in the resina draconis extract+HIF-1α inhibitor group, the levels of TNF-α, IL-6, and IL - β were lower in the resina draconis extract+HIF-1α inhibitor group (P<0.05).Conclusion: The intervention of resina draconis extract on perianal abscess rats can promote wound healing by regulating the HIF-1α/VEGF signaling pathway, and has a good therapeutic effect. 2024 Vol. 30 (12): 1958-1965 [Abstract] ( 41 ) HTML (1 KB)  PDF (2837 KB)  ( 6 ) 1965 Effect of Baicalin on the Inflammatory Response of Lung Tissue in Severe Pneumonia Rats by Regulating the TGF-β1/Smad Signaling Pathway YANG Zongyu, CAI Wei, XIA Chen, et al DOI: 10.3969/j.issn.1006-6233.2024.12.05 Objective: To investigate the effect of baicalin (BC) on the inflammatory response of lung tissue and the transforming growth factor-β1 (TGF-β1)/Smad signaling pathway in severe pneumonia (SP) rats.Methods: SD rats were randomly separated into a control group, a model group (SP group), low, medium, and high-dose baicalin groups (BC-L, BC-M, BC-H groups), and a high-dose baicalin+pathway activator group (BC-H+SRI-011381 group), with 18 rats in each group. Except for the control group, all other rats were used to construct an SP rat model. The lung coefficient (LI) and wet/dry weight ratio (W/D) of rats in each group were measured. ELISA was applied to detect the levels of inflammatory factors and severe pneumonia related biomarkers procalcitonin (PCT) and C-reactive protein (CRP) in alveolar lavage fluid. Wright's staining was applied to detect the number of neutrophils (NEU), eosinophils (EOS), and lymphocytes (LYM) in the sediment of alveolar lavage fluid. HE staining was applied to observe the pathological morphology of lung tissue. Western blot was applied to detect the expression of TGF-β1/Smad signaling pathway related proteins.Results: Compared with control group, SP group showed damage in lung tissue structure, with some areas of alveolar edema showing bleeding, thickening of alveolar walls and lung interstitium, and infiltration of a large number of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β1, p-Smad2/Smad2, and p-Smad3/Smad3 were elevated (P<0.05). Compared with SP group, BC-L, BC-M, and BC-H groups showed improvement in lung tissue structure damage, with relatively normal alveolar morphology, reduced edema, and reduced infiltration of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β1, p-Smad2/Smad2, and p-Smad3/Smad3 were reduced (P<0.05). Compared with BC-H group, BC-H+SRI-011381 group showed more severe damage to lung tissue structure and alveolar edema, with increased infiltration of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β1, p-Smad2/Smad2, and p-Smad3/Smad3 were elevated (P<0.05).Conclusion: Baicalin can improve lung tissue inflammation in SP rats, and its mechanism of action is related to the inhibition of TGF-β1/Smad pathway. 2024 Vol. 30 (12): 1965-1971 [Abstract] ( 27 ) HTML (1 KB)  PDF (1614 KB)  ( 6 ) 1971 microRNA-138 Alleviates Pelvic Organ Prolapse in Rats by Regulating FBLN5/IL-1β Pathway YUAN Mengwei, YAN Ziyan, MAO Yi, et al DOI: 10.3969/j.issn.1006-6233.2024.12.06 Objective: To analyze the mechanism of microRNA-138 in alleviating pelvic organ prolapse in rats by regulating the FBLN5/IL-1β pathway.Methods: Forty SPF grade SD female rats with three natural childbirths were selected, of which 10 were used as the control group, and another 30 were used to construct a pelvic organ prolapse model. After anesthesia, the vagina of the rats was opened and the urinary catheter was sutured to the vaginal opening to make it droop. Two weeks later, bilateral oophorectomy was performed. A total of 27 rats were successfully modeled and divided into model group (n=9), miRNA-138 inhibitor group (n=9), and miRNA-138 inhibitor+FBLN5 inhibitor group (n=9). The control group and model group were injected physiological saline, the miRNA-138 inhibitor group was injected 10μL of miR-138 inhibitor lentivirus suspension, and the miRNA-138 inhibitor+FBLN5 inhibitor group was injected 10 μ l of miR-138inhibitor lentivirus suspension. inhibitor+10μL FBLN5 inhibitor. The histopathological changes were observed, as well as fibroblast count, urodynamics, expression levels of type I and type Ⅲ collagen, FBLN5/IL-1β - related mRNA expression levels, and FBLN5/IL-1β expression levels in each group of rats.Results: Compared with the control group, the number of fibroblasts in the model decreased, and the basal bladder pressure, bladder voiding volume, bladder voiding pressure, bladder peak pressure, expression levels of type I collagen, type Ⅲ collagen, FBLN5 related mRNA expression, and FBLN5 expression decreased. The expression levels of IL-1β-related mRNA and IL-1β increased. Compared with the model group, the miRNA-138 inhibitor group and miRNA-138 inhibitor+FBLN5 inhibitor group had an increase in the number of fibroblasts and an increase in bladder basal pressure, bladder voiding volume, bladder voiding pressure, bladder peak pressure, type I collagen, type Ⅲ collagen expression, FBLN5 related mRNA expression, and FBLN5 expression. However, the expression of IL-1β-related mRNA and IL-1β-related mRNA decreased, and miRNA-138 inhibitor group had a larger number of fibroblasts. Bladder basal pressure, bladder voiding volume, bladder voiding pressure, bladder peak pressure, type I collagen, type Ⅲ collagen expression, FBLN5 related mRNA expression, and FBLN5 expression were higher, while IL-1β-related mRNA expression and IL-1β-related mRNA expression were low (P<0.05).Conclusion: Inhibiting the expression of microRNA-138 can regulate the FBLN5/IL-1β pathway, promote the expression of fibroblasts and collagen, improve rat urodynamics, and alleviate pelvic organ prolapse. 2024 Vol. 30 (12): 1971-1978 [Abstract] ( 30 ) HTML (1 KB)  PDF (2288 KB)  ( 9 ) 1978 Effect of Curcumin on Wound Healing of Diabetic Foot Ulcer Based on TGF-β1/Smad3 Signaling Pathway RUN Jingmo, et al DOI: 10.3969/j.issn.1006-6233.2024.12.07 Objective: To investigate the effects of curcumin on transforming growth factor-β (TGF-β)/Smad3 signaling pathway, wound healing, cellular inflammatory factors and angiogenesis in diabetic foot ulcer (DFU) rats. Methods: All rats were randomly divided into control group, DFU model group (DFU group), curcumin treatment group (curcumin group) and curcumin treatment +Smad3 inhibitor SIS3 group (SIS3 group). The wound healing rate of rats was calculated. The hematoxylin-eosin (H&E) staining was used to observe the pathological changes of wound tissue. The enzyme-linked immunosorbent assay (ELISA) was used to detect interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels. Western blot was used to analyze vascular endothelial growth factor (VEGF), CD31 and TGF-β1/Smad3 signaling protein expression. Results: Compared with those of control group, the rats in DFU group had significantly decreased wound healing rate and the protein expressions of TGF-β1 and Smad3, significantly increased the levels of IL-1β and TNF-α (P<0.05), and with a large number of inflammatory cells in the wound tissue and reduced neovascularization. Compared with those of DFU group, the rats in curcumin group presented significantly increased wound healing rate and the protein expressions of TGF-β1 and Smad3, significantly decreased the levels of IL-1β and TNF-α (P<0.05). The inflammatory cells in the wound tissue gradually decreased, and the number of new blood vessels gradually increased. After adding Smad3 inhibitor, the effect of curcumin on wound tissue injury in DFU rats can be eliminated to a certain extent. Conclusion: Curcumin may inhibit inflammatory response and induce angiogenesis by activating TGF-β1/Smad3 signaling pathway, thereby promoting wound healing in UFD rats. 2024 Vol. 30 (12): 1978-1982 [Abstract] ( 25 ) HTML (1 KB)  PDF (1380 KB)  ( 4 ) 1982 Molecular Mechanism of SHH/GLI1 Signaling Pathway Regulating Macrophage Pyroptosis in Acute Myocardial Infarction LIAN Ming, GONG Fangna, ZHAO Wanglei DOI: 10.3969/j.issn.1006-6233.2024.12.08 Objective: To explore the possible molecular mechanism of sonic hedgehog (SHH) / glioma-associated oncogene 1 (GLI1) signaling pathway regulating macrophage pyroptosis in acute myocardial infarction (AMI). Methods: Fifty rats were randomly divided into sham operation group, AMI group, SHH inhibition group, macrophage clearance group, macrophage clearance + SHH inhibition group, with 10 rats in each group. Myocardial tissue and serum were collected. Cultured macrophages Raw264.7 were divided into control group, hypoxia group and SHH inhibition group. Masson staining was used to detect myocardial fibrosis level and infarct size. The protein levels of SHH, GLI1, NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3), gasdermin-D (GSDMD) were detected by Western blotting. The mRNA levels of SHH, GLI1 and F4/80 were detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). Serum creatine kinase-myocardial band (CK-MB) and lactate dehydrogenase (LDH) were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with sham operation group, myocardial fibrosis level, infarct size, SHH and GLI1 protein expression, serum CK-MB and LDH contents were significantly increased in AMI group (P<0.05). The expressions of NLRP3, Caspase-1 and GSDMD proteins in myocardial tissue were significantly increased (P<0.05), and the F8/40 mRNA levels in myocardial tissue of macrophage clearance group were significantly decreased (P<0.05), indicating successful macrophage clearance. Compared with AMI group, the myocardial fibrosis level, infarct size and protein expressions of SHH, GLI1, NLRP3, Caspase-1 and GSDMD were significantly decreased in the inhibited SHH group (P<0.05), and the serum CK-MB and LDH contents in the macrophage clearance group were significantly decreased (P<0.05). The serum CK-MB and LDH contents of rats in the macrophage clearance + SHH inhibition group were compared to those in the macrophage clearance group (P<0.05). Compared with the control group, the mRNA levels of SHH and GLI1and the protein expressions of NLRP3, Caspase-1 and GSDMD in macrophages of hypoxia group were significantly increased (P<0.05). Compared with hypoxia group, the mRNA levels of SHH and GLI1 and the protein expressions of NLRP3, Caspase-1 and GSDMD in inhibited SHH group were significantly decreased (P<0.05). Conclusion: Down-regulation of SHH/GLI1 signaling pathway can inhibit macrophage pyroptosis and improve AMI. 2024 Vol. 30 (12): 1982-1987 [Abstract] ( 30 ) HTML (1 KB)  PDF (1690 KB)  ( 6 ) 1987 Effect of Pachymic Acid on Endothelial Cell Damage in Essential Hypertension Rats by Regulating the IL-6/JAK2/STAT3 Signaling Pathway GAO Xiurong, TONG Xingrui, WANG Qinghai, et al DOI: 10.3969/j.issn.1006-6233.2024.12.09 Objective: To investigate the effect of pachymic acid (PA) on endothelial cell damage in essential hypertension (EHT) rats by regulating the interleukin-6 (IL-6)/tyrosine kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway. Methods: Sixty EHT rats were randomly separated into EHT group, low, high concentration PA group, benazepril group, high concentration PA+rrIL-6 group, with 12 rats in each group. Additional 12 Wistar-Kyoto (WKY) rats were selected as the control group. The changes in diastolic blood pressure (DBP), systolic blood pressure (SBP), and mean arterial pressure (MAP) of rats were detected. Enzyme-linked immunosorbent assay (ELISA) was applied to detect levels of endothelin-1 (ET-1), intercellular adhesion molecule-1 (ICAM-1), and von Willebrand factor (vWF) in serum. The hematoxylin and eosin (H&E) staining was applied to detect the pathology of the thoracic aorta. Rat umbilical vein endothelial cells (UVECs) were separated into Vitro-control group, Vitro-EHT group, Vitro-low concentration PA group, Vitro-high concentration PA group, Vitro-Benazepril group, and Vitro-high concentration PA+rrIL-6 group. ELISA was applied to detect the levels of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), and IL-1β in the supernatant of UVECs. Flow cytometry was applied to detect UVECs apoptosis. Western blot was applied to detect the proteins levels of IL-6, p-STAT3, and p-JAK2 in UVECs. Results: Compared with the control group, the endometrial structure of the thoracic aorta in the EHT group was abnormal, with defects or protrusions, and the boundary between the endometrium and outer membrane was blurred, the levels of DBP, SBP, MAP, and serum ET-1, ICAM-1, and vWF were significantly increased (P<0.05). Compared with the EHT group, the pathological damage of the thoracic aorta in rats in the low, high concentration PA, and benazepril group was significantly improved, the levels of DBP, SBP, MAP, and serum ET-1, ICAM-1, and vWF were significantly decreased (P<0.05). Compared with the Vitro-control group, the levels of TNF-α, IL-1β in the supernatant, the apoptosis rate of UVECs, and the proteins levels of IL-6, p-STAT3, and p-JAK2 in UVECs in the Vitro-EHT group were significantly increased, while the level of IL-10 was significantly decreased (P<0.05). Compared with the Vitro-EHT group, the levels of TNF-α, IL-1β in the supernatant, apoptosis rate of UVECs, and IL-6, p-STAT3, and p-JAK2 proteins in UVECs in the Vitro-low concentration PA group, the Vitro-high concentration PA group, and the Vitro-Benazepril group were significantly decreased, while the level of IL-10 was significantly increased (P<0.05). RrIL-6 reversed the inhibitory effects of high concentrations PA on endothelial injury, Ang Ⅱ induced UVECs inflammation and inhibited apoptosis in EHT rats. Conclusion: PA may inhibit endothelial cell damage in EHT rats by inhibiting the IL-6/JAK2/STAT3 pathway. 2024 Vol. 30 (12): 1987-1993 [Abstract] ( 24 ) HTML (1 KB)  PDF (1781 KB)  ( 7 ) 1993 Base on YAP/TAZ Signaling Pathway to Investigate the Effect of Atractylenolide I on Intestinal Barrier Dysfunction in UC Rats LI Sen, TIAN Lihui, HAN Tao, et al DOI: 10.3969/j.issn.1006-6233.2024.12.010 Objective: To investigate the effects of atractylenolide I (Atr-I) on intestinal barrier dysfunction and Yes-associated protein (YAP)/transcription coactivator containing PDZ binding motif (TAZ) signaling pathway in rats with ulcerative colitis (UC).Methods: UC rat model was constructed,all experimental rats were divided into UC group,17.5,35.0 mg/kg Atr-I dose group (Atr-I-L,Atr-I-H group),35.0 mg/kg Atr-I+17.5 mg/kg Yap inhibitor vitipofin group (Atr-I-H+VTPF group),18 rats in each group,and 18 rats were taken as control group (Control group).After the experiment,the body weight of mice of each group was measured and the disease activity index (DAI) was calculated after intervention.The length of the colon was measured and the pathological morphology changes of the colojisun in each group were observed by hematoxylin-eosin (HE) staining.Serum interleukin (IL)-1β,IL-17 and interferon-γ (IFN-γ) were detected with enzyme-linked immunosorbent assay (ELISA).The positive expression of intestinal mucosal barrier-related protein band-closing protein 1 (ZO-1) and claudin-1 (Claudin-1) in colon tissue of rats of each group were detected by immunohistochemistry.The protein expression levels of YAP,p-YAP and TAZ in colon tissues were detected by western blotting (Western blot).Results: Compared to Control group,the colonic mucosal epithelial cells in UC group were disordered,the goblet cells were significantly reduced,a large amount of infiltration inflammatory cells were found,and ulcers were formed in some areas,even thickening and adhesion,intestinal deformation,mucosal congestion and edema were obvious,body weight,colon length,ZO-1,Claudin-1 positive expression,p-YAP/YAP,and TAZ expression were significantly decreased,and the DAI score,IL-1β,IL-17,and IFN-γ levels were obviously increased (P<0.05).Compared with UC group,the colonic mucosal epithelial injury of rats in Atr-I-L and Atr-I-H groups was improved,body weight,colon length,positive expression of ZO-1,Claudin-1,p-YAP/YAP and TAZ were significantly increased in turn,and the DAI score,IL-1β,IL-17 and IFN-γ levels were significantly decreased in turn (P<0.05).Compared with Atr-I-H group,the colonic mucosal epithelial injury in Atr-I-H+VTPF group was aggravated,body weight,colon length,ZO-1,Claudin-1 positive expression,p-YAP/YAP,and TAZ expression were significantly decreased,and the DAI score,IL-1β,IL-17,and IFN-γ levels were obviously increased (P<0.05).Conclusion: Atr-I can reduce DAI score,inhibit inflammatory response,improve colonic mucosal epithelial injury,and improve intestinal barrier dysfunction in UC rats,the mechanism may be related to the activation of YAP/TAZ signaling pathway. 2024 Vol. 30 (12): 1993-1999 [Abstract] ( 18 ) HTML (1 KB)  PDF (3299 KB)  ( 34 ) 1999 Physiological and Biochemical Characteristics and Transcriptome Analysis of EBV Infected Jurkat T Cells SHI Rui, CHAI Wenjia, GUI Jingang, et al DOI: 10.3969/j.issn.1006-6233.2024.12.011 Objective: This study aims to investigate the mechanism of EBV infection in T cells by sequencing the transcriptome of EBV-stimulated Jurkat T cells overexpressing CD21. Methods: The target gene CD21 was cloned by PCR and inserted into an overexpression lentiviral vector via molecular cloning techniques, ensuring its stable overexpression on the surface of Jurkat T cells. The CD21-overexpressing Jurkat T cells were then stimulated with EBV(EBV-LV-Jurkat). Transcriptome sequencing was performed on EBV-stimulated CD21-overexpressing Jurkat T cells, untreated Jurkat T cells, and Jurkat T cells infected with lentivirus alone(LV-Jurkat). Gene set enrichment analysis was conducted on differentially expressed genes (DEGs) between EBV-stimulated CD21-overexpressing Jurkat T cells (EBV-LV-Jurkat T) and CD21-overexpressing Jurkat T cells (LV-Jurkat T). Results: GO enrichment analysis of DEGs between EBV-LV-Jurkat T and LV-Jurkat T revealed significant enrichment in the molecular function of MAP kinase tyrosine phosphatase activity , involving the DUSP1, DUSP10, DUSP5, and DUSP8 genes. KEGG pathway enrichment analysis of DEGs indicated that multiple pathways and molecules, similar to those observed in EBV-infected B cells, were significantly enriched in the MAPK, IL-17, HIF-1, NF-κB, and PI3K/AKT signaling pathways, and genes related to the immune response system. Conclusion: This study successfully constructed a model of CD21-overexpressing Jurkat T cells and revealed the impact of EBV stimulation on significantly activating the MAPK signaling pathway, providing crucial preliminary data for further exploration of the mechanism of EBV infection in T cells. 2024 Vol. 30 (12): 1999-2005 [Abstract] ( 27 ) HTML (1 KB)  PDF (2836 KB)  ( 114 ) 2005 Predictive Value of Uric Acid Combined with Inflammatory Markers on Coronary Blood Flow Reserve and MACE in Coronary Heart Disease SONG Mingzhe, ZHU Wangliang, LI Jianfei, et al DOI: 10.3969/j.issn.1006-6233.2024.12.012 Objective: To explore the predictive value of serum uric acid (SUA) combined with neutrophil to lymphocyte ratio (dNLR) on coronary blood flow reserve and major cardiovascular adverse events (MACE) in coronary artery disease (CAD).Methods: A total of 323 stable CAD patients who received percutaneous coronary intervention (PCI) in our hospital from January 2020 to December 2021 were selected as the research subjects. DNLR is defined as neutrophil count /(WBC count-neutrophil count). The blood flow reserve fraction (FFR) was quantitatively analyzed by coronary angiography. The end point of this study was MACE, including all-cause mortality and readmission of severe heart failure during follow-up.Results: Compared with the group without MACE, the cases of WBC count, neutrophil count, dNLR, SUA and FFR≤0.92 in MACE group were significantly higher (P<0.05), and the FFR was significantly lower (P<0.05). SUA (HR=1.886, 95%CI=1.545~2.303), dNLR (HR=1.479, 95%CI=1.241~1.762), FFR≤0.92(HR=3.208, 95%CI=1.468) were the influencing factors of MACE events in CAD patients (P<0.05). There was a significant negative correlation between SUA and FFR (r=-0.163, P=0.003). The combination of SUA and dNLR had the greatest ability to predict MACE of CAD patients undergoing PCI, with AUC of 0.908, sensitivity of 96.7% and specificity of 79.9%. According to ROC curve, the optimal critical values of SUA and dNLR were divided into subgroup 1(n=180), subgroup 2(n=116) and subgroup 3(n=27). There were 1 case, 13 cases and 16 cases in each subgroup, and the incidence rates were 99.4%, 88.8% and 40.7% respectively. Kaplan-Meier survival curve analysis showed that the incidence of MACE events increased significantly from subgroup 1 to subgroup 3 (Log Rank (Mantel-Cox)=94.912, P<0.001).Conclusion: In CAD patients receiving PCI, the increase of dNLR and SUA is independently related to the high risk of MACE, and the potential clinical utility of the combination of DNLR and SUA in identifying long-term MACE in CAD patients is emphasized. 2024 Vol. 30 (12): 2005-2011 [Abstract] ( 28 ) HTML (1 KB)  PDF (1444 KB)  ( 99 ) 2011 Influence of Contra-Incision Combined with High Loose-Tight Thread on IL-6 IL-17 and Wound Healing Time in Patients with High Complex anal Fistula XIE Yongjun, MU Yongbao, ZHANG Yan, et al DOI: 10.3969/j.issn.1006-6233.2024.12.013 Objective: To explore the influence of contra-incision combined with high loose-tight thread on the levels of interleukin-6 (IL-6) and interleukin-17 (IL-17) and wound healing time in patients with high complex anal fistula (CAF).Methods: The clinical data of 130 patients with high CAF who received surgical treatment in the hospital were retrospectively analyzed from January 2021 to October 2023. According to the differences in surgical methods, the subjects were divided into contra-incision group (contra-incision combined with high loose-tight thread, n=68) and traditional group (traditional incision and thread-drawing, n=62). The therapeutic effect, postoperative wound healing status, wound symptoms scores and levels of IL-6 and IL-17 before surgery and at 1 week after surgery, Wexner anal incontinence score and anal sphincter function indexes [anal resting pressure (ARP), anal maximum contraction pressure (AMCP)] before surgery and at 3 months after surgery were compared between the contra-incision group and the traditional group, and the incidence of complications was recorded.Results: There was no statistical significance in the total effective rate of treatment between contra-incision group and traditional group (98.53% vs 95.16%) (P>0.05). Compared with the traditional group, the postoperative wound healing time and hospitalization time in the contra-incision group were shorter, and the wound healing rate was higher (P<0.05). At 1 week after surgery, the wound symptoms scores in both groups were reduced, and the reductions were greater in the contra-incision group (P<0.05). The levels of IL-6 and IL-17 were decreased in both groups at 1 week after surgery, and the decreases in the contra-incision group were greater compared to the traditional group (P<0.05). The Wexner anal incontinence score, AMCP and ARP at 3 months after surgery were declined in the two groups, and the declines in the contra-incision group were greater (P<0.05). No statistical difference was shown in the incidence of urinary retention between the contra-incision group and the traditional group (1.47% vs 3.23%) (P>0.05).Conclusion: Both contra-incision combined with high loose-tight thread and traditional surgery have good effects in the treatment of high CAF. Both the two methods have high safety and can effectively reduce the influence of surgery on the anal sphincter. However, compared with the traditional surgery, contra-incision combined with high loose-tight thread can more significantly shorten the wound healing time, accelerate the recovery of wound-related symptoms, and down-regulate the body’ s inflammation level, and it is more conducive to accelerating the rehabilitation process of patients, and can be the first choice for the treatment of high CAF. 2024 Vol. 30 (12): 2011-2016 [Abstract] ( 25 ) HTML (1 KB)  PDF (1124 KB)  ( 4 ) 2017 The Relationship of Serum Levels of Il-23 Uch-L1 and Mir-424 with Severity of Hypoxic-Ischemic Encephalopathy (Hie) in Full-Term Newborns and the Predictive Value on Prognosis ZHANG Juan, et al DOI: 10.3969/j.issn.1006-6233.2024.12.014 Objective: To investigate the relationship of serum interleukin-23 (IL-23), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) and micrnas 424 (miR-424) with the severity of hypoxic-ischemic encephalopathy (HIE) in full-term neonates and their predictive value on prognosis.Methods: A total of 105 cases of full-term infants with HIE diagnosed in the period from January 2019 to December 2022 were selected as the HIE group, which were further divided into mild (37 cases), moderate (43 cases), and severe (25 cases) subgroups according to the HIE grading criteria. Additionally, 105 cases of full-term healthy newborns from the same period were selected as the control group. Venous blood samples were collected from all subjects within one day of enrollment, and serum levels of IL-23 and UCH-L1 were measured using enzyme-linked immunosorbent assay (ELISA), while serum miR-424 levels were determined using real-time quantitative polymerase chain reaction (qRT-PCR). The serum levels of IL-23, UCH-L1, and miR-424 were compared between the HIE group and the control group, and the correlation between these markers and the severity of HIE was analyzed. The HIE group was followed up for one year, and according to the prognosis, they were further divided into the good prognosis subgroup and the poor prognosis subgroup. The serum levels of IL-23, UCH-L1, and miR-424 were compared between these two subgroups, and the predictive value of these markers for prognosis was analyzed.Results: The serum levels of IL-23 (68.67±21.29) ng/L and UCH-L1 (1.98±0.63) ng/mL in the HIE group were higher than those in the control group (30.06±6.13) ng/L and (0.75±0.24) ng/mL, respectively. Meanwhile, the serum level of miR-424 (1.98±0.63) was lower than that in the control group (1.02±0.11) (P<0.05). In the severe HIE subgroup, the serum levels of IL-23 (95.21±21.08) ng/L and UCH-L1 (2.42±0.46) ng/mL were higher than those in the moderate HIE subgroup (70.49±18.13) ng/L and (2.01±0.40) ng/mL, respectively, and those in the mild HIE subgroup (48.62±14.72) ng/L and (1.65±0.34) ng/mL. The moderate HIE subgroup had higher serum levels of IL-23 and UCH-L1 than the mild HIE subgroup, while the serum level of miR-424 (0.53±0.10) was lower than that in the moderate HIE subgroup (0.70±0.12) and mild HIE subgroup (0.84±0.16), with the moderate HIE subgroup having lower serum levels of miR-424 than the mild HIE subgroup (P<0.05). Serum levels of IL-23 and UCH-L1 positively correlate with the severity of the disease, while miR-424 levels negatively correlate with it (P<0.05). After a 1-year follow-up, 1 case was lost. Among the 104 children, 82 had a good prognosis and 22 had a poor prognosis. The poor prognosis subgroup had lower Apgar scores at 1 and 5 minutes, as well as lower NBNA scores, and a higher severity of illness compared to the good prognosis subgroup (P<0.05). The serum levels of IL-23 (94.41±20.35) ng/L and UCH-L1 (2.46±0.62) ng/mL in the poor prognosis subgroup were higher than those in the good prognosis subgroup (61.76±18.74) ng/L and (1.84±0.49) ng/mL, respectively, while miR-424 levels (0.51±0.16) were lower than those in the good prognosis subgroup (0.75±0.20) (P<0.05). Partial correlation analysis showed that, after controlling for other factors, serum levels of IL-23, UCH-L1, and miR-424 were significantly associated with the prognosis of full-term HIE patients (P<0.05). The AUCs for predicting the prognosis of full-term HIE patients using serum levels of IL-23, UCH-L1, and miR-424 were 0.815, 0.792, and 0.806, respectively. The AUC for combined prediction was 0.913, with a sensitivity of 81.82% and a specificity of 86.59%, which was superior to the predictive value of each individual indicator.Conclusion: Serum IL-23, UCH-L1 and miR-424 are significantly correlated with the severity of full-term neonates with HIE, and have independent prognostic ability, and the combined prognostic value is more reliable. 2024 Vol. 30 (12): 2017-2023 [Abstract] ( 21 ) HTML (1 KB)  PDF (1281 KB)  ( 6 ) 2023 Association of TNF-α Gene Polymorphisms with Pulmonary Infection After Heart Valve Replacement GU Xintong, et al DOI: 10.3969/j.issn.1006-6233.2024.12.015 Objective: To investigate the correlation between lung infection and tumor necrosis factor-alpha (TNF-α) gene polymorphism after heart valve replacement (HVR) surgery. Methods: A total of 80 patients with HVR in Nanjing Drum Tower Hospital from January 2020 to December 2023 were retrospectively selected, and they were divided into lung infection group (30 cases) and non-lung infection group (50 cases) according to the presence or absence of postoperative lung infection. Results: A total of 43 strains of pathogenic bacteria were detected in 30 patients with pulmonary infection, mainly gram-negative bacteria (62.79%), with Escherichia coli (20.93%). Followed by Gram-positive bacteria (25.58%), with Staphylococcus aureus (13.95%). Finally, fungi accounted for 11.63%, with Candida alba (9.30%). The lung infection group had significantly higher the proportion of age, hyperlipidemia, diabetes and cerebral infarction, significantly more the amount of intraoperative blood loss, and significantly longer the duration of ventilator use, cardiopulmonary bypass and ICU stay compared to the non-lung infection group (all P<0.05). The lung infection group presented significantly lower the proportion of the left ventricular ejection fraction, GG genotype frequency of TNF-α gene rs1800629, and G allele frequency and significantly higher the proportion of GA, AA and A relative to the non-lung infection group (all P<0.05). The influencing factors of postoperative pulmonary infection included ICU stay time, extracorporeal circulation time, TNF-α gene rs1800629 site AA (P<0.05). Conclusion: There is a significant correlation between lung infection after HVR and TNF-α gene polymorphism, and the latter is a predisposing factor for the former. 2024 Vol. 30 (12): 2023-2028 [Abstract] ( 33 ) HTML (1 KB)  PDF (1108 KB)  ( 11 ) 2028 Relationship between Expression Levels of Serum NOX4 and MUC1 with Radiotherapy Efficacy and 1-Year Overall Survival Rate in Patients with Stage Ⅲ Non-Small Cell Lung Cancer LU Jue, CAI Mengjiao, ZHANG Yingbing DOI: 10.3969/j.issn.1006-6233.2024.12.016 Objective: To study the relationship between expression levels of serum nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4) and mucin 1 (MUC1) with radiotherapy efficacy and overall survival rate of 1-year follow-up in patients with stage Ⅲ non-small cell lung cancer (NSCLC). Methods: Totally 102 patients with stage Ⅲ NSCLC in the hospital from April 2020 to March 2023 were selected for radiotherapy intervention, and serum NOX4 and MUC1 levels were detected before radiotherapy. Another 51 healthy volunteers were selected as controls. Before radiotherapy, the inter-group levels of serum NOX4 and MUC1 were compared, and the intra-class levels of serum NOX4 and MUC1 in patients stage Ⅲ NSCLC with different clinical characteristics were assessed. According to the radiotherapy effect, patients with stage Ⅲ NSCLC were divided into good efficacy group and poor efficacy group, and the levels of serum NOX4 and MUC1 were compared after radiotherapy. All patients were followed up, and the 1-year overall survival rates of different patients were compared using the median levels of serum NOX4 and MUC1. Univariate and multivariate Cox regression analyses were used to analyze the influencing factors of 1-year survival rate in patients with stage Ⅲ NSCLC. Results: The levels of serum NOX4 and MUC1 in NSCLC group were significantly higher than those in control group (P<0.05). There were no statistical differences in serum NOX4 and MUC1 levels among stage Ⅲ NSCLC patients with different genders, ages, body mass index (BMI) values, tumor locations, maximum tumor diameters and pathological types before radiotherapy (P>0.05). Among 102 patients with stage Ⅲ NSCLC, the radiotherapy results showed that 55 patients were in the good efficacy group and 47 patients were in the poor efficacy group. Serum levels of NOX4 and MUC1 in good efficacy group were significantly lower than those in poor efficacy group (P<0.05). The 1-year overall survival rate in high-level NOX4 group was significantly lower than that in low-level NOX4 group (P<0.05), which in high-level MUC1 group was significantly lower than that in low-level MUC1 group (P<0.05). Univariate and multivariate Cox regression analyses showed that serum NOX4 and MUC1 levels were the influencing factors of 1-year survival rate in patients with stage Ⅲ NSCLC after radiotherapy (P<0.05). Conclusion: The expression levels of serum NOX4 and MUC1 in patients with stage Ⅲ NSCLC are related to the radiotherapy efficacy and overall survival rate after one year of follow-up. The detection of serum NOX4 and MUC1 levels is helpful to evaluate the radiotherapy efficacy and early prognosis survival. 2024 Vol. 30 (12): 2028-2033 [Abstract] ( 21 ) HTML (1 KB)  PDF (1351 KB)  ( 8 ) 2033 Correlation between Epstein-Barr Virus DNA Load and Liver Function Damage in Children with Infectious Mononucleosis LU Lu, JIANG Lina, WANG Yinghui, et al DOI: 10.3969/j.issn.1006-6233.2024.12.017 Objective: To investigate the relationship between Epstein-Barr virus (EBV)-deoxyribonucleic acid (DNA) load and liver function damage in children with infectious mononucleosis (IM). Methods: Totally 126 IM children who were diagnosed in department of pediatrics of our hospital from February 2016 to March 2023 were included as the IM group, and 50 healthy children during the same period as the control group. The liver function indexes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) were compared between the control group and the IM group. Children in the IM group were divided into liver damage subgroup (76 cases) and non-liver damage subgroup (50 cases) according to the presence or absence of liver function damage, and the levels of liver function indexes were compared. The correlation between EBV-DNA load and liver function damage was assessed by Spearman rank correlation analysis, and receiver operating characteristic (ROC) curve was drawn to analyze the predictive ability of EBV-DNA load and liver function damage. Results: The levels of ALT, AST, and TBIL in the IM group were significantly increased, and which were significantly higher than those in the control group (P<0.05). The levels of ALT, AST and TBIL and EBV-DNA load were significantly higher in the liver damage subgroup compared to the non-liver damage subgroup (P<0.05). Spearman rank correlation analysis showed that EBV-DNA load was positively correlated with ALT, AST, and TBIL (r=0.652, 0.628, 0.634, respectively; all P<0.05). Logistic regression analysis demonstrated that increased ALT, AST, TBIL and EBV-DNA load were influencing factors for liver function damage. ROC curve analysis results revealed that the area under the curve (AUC) of EBV-DNA load, ALT, AST, and TBIL alone in predicting liver function damage was 0.868, 0.830, 0.821 and 0.681, respectively, and while the AUC of combined prediction was 0.962, with the sensitivity of 0.908 and the specificity of 0.920. Conclusion: EBV-DNA load is closely related to the degree of liver function damage in IM children, and the risk of liver function damage is higher in the high load group, suggesting that the liver function indexes should be closely monitored in clinical management to relieve the risk of liver damage. 2024 Vol. 30 (12): 2033-2038 [Abstract] ( 28 ) HTML (1 KB)  PDF (1285 KB)  ( 88 ) 2038 Effects of LYR Ferritin and S-LDH on the Prognosis of Children with Newly Diagnosed Acute Promyelocytic Leukemia and its Early Warning Significance LEI Qingling, LIN Yunbi, HUANG Tilong, et al DOI: 10.3969/j.issn.1006-6233.2024.12.018 Objective: To investigate the effects of circulating lymphocyte ratio (LYR), ferritin and serum-lactate dehydrogenase (S-LDH) on the prognosis of children with newly diagnosed acute promyelocytic leukemia (APL)and its early warning significance. Methods: A total of 89 children with newly diagnosed APL admitted to our hospital from January 2018 to December 2023 were divided into complete response (CR) group and non-CR group according to the prognosis after treatment. Clinical data, levels of LYR, ferritin and S-LDH were compared between the two groups, and the net correlation between LYR, ferritin and S-LDH with disease outcome was analyzed. Receiver operating characteristic (ROC) curves were conducted to analyze the value of LYR, ferritin and S-LDH in predicting the disease outcome, and evaluate the effects of different expression levels of LYR, ferritin and S-LDH on the disease outcome. Results: The LYR in non-CR group was significantly lower than that in CR group, and the ferritin and S-LDH were significantly higher than those in CR group (P<0.05). LYR, ferritin and S-LDH were significantly correlated with the disease outcome (P<0.05), and the net correlation coefficients of LYR, ferritin and S-LDH were -0.825, 0.860 and 0.812, respectively. The optimal cut-off values of LYR, ferritin and S-LDH were 34.12, 261.22 and 511.39, respectively, and the area under the ROC curve (AUC) was 0.779, 0.802 and 0.797, respectively. Odds ratio (OR) for risk analysis showed that the risk of non-CR in patients with high LYR, ferritin and S-LDH expression was 0.120, 12.783 and 9.579 times higher than that in patients with low expression, respectively. The AUC of combined prediction of LYR, ferritin, and S-LDH for non-CR was 0.916 (95%CI: 0.838-0.964), with the sensitivity of 88.89%, and the specificity of 83.10%. Conclusion: The levels of LYR, ferritin and S-LDH are closely related to the disease outcome of APL patients, which provide a reference for clinical evaluation and prediction in early APL patients, formulating corresponding intervention programs to improve the prognosis. 2024 Vol. 30 (12): 2038-2043 [Abstract] ( 14 ) HTML (1 KB)  PDF (1340 KB)  ( 9 ) 2043 Correlation between Asthma Attack Frequency and Pulmonary Surfactant Protein Level in Preschool Children with Bronchial Asthma MAO Hua, ZHAO Xiaoyuan, SUN Huanxin, et al DOI: 10.3969/j.issn.1006-6233.2024.12.019 Objective: To investigate the correlation between asthma attack frequency and pulmonary surfactant protein(SP) level in preschool children with bronchial asthma(BA). Methods: A total of 112 preschool children with BA (disease group) diagnosed in the hospital from February 2020 to October 2023 were enrolled, and children of disease group were divided into high-frequency subgroup (n=52) and low-frequency subgroup (n=60) based on the asthma attack frequency. At the same time, 60 healthy children of the same age with normal physical examination in our hospital were regarded as the control group. The pulmonary surfactant protein A (SP-A), pulmonary surfactant protein D (SP-D), Asthma Control Test (ACT) scores, pulmonary function indexes (forced expiratory volume in one second [FEV1], forced vital capacity [FVC], FEV1/FVC) were included as comparators between groups. Spearman rank correlation analysis was used to evaluate the correlation between asthma attack frequency with SP level and pulmonary function, and receiver operating characteristic (ROC) curve was drawn to analyze the predictive ability of SP for asthma attack. Results: The disease group had significantly higher SP-A and SP-D levels, and lower ACT score and pulmonary function indexes relative to the control group (all P<0.05). The high-frequency subgroup presented significantly increased SP-A and SP-D levels, decreased ACT score and levels of FEV1, FVC and FEV1/FVC compared to the low-frequency subgroup (all P<0.05). Univariate analysis showed that SP-A, SP-D, FEV1, FVC and FEV1/FVC were correlated with the asthma attack frequency (P<0.05). Multivariate logistic regression analysis indicated that SP-A and SP-D were independent risk factors for the asthma attack frequency (P<0.05). ROC curve analysis indicated that the area under the curve (AUC) of SP-A and SP-D alone in predicting asthma attack frequency was 0.784 and 0.816, respectively (P<0.05). The AUC combined prediction were 0.984, with the sensitivity of 0.942 and the specificity of 0.950. Conclusion: The asthma attack frequency is closely related to SP level in preschool children with BA. SP level can be used as a predictor of asthma attack frequency. Pulmonary function indicators are useful for assessing the respiratory status, facilitating implementation of early interventions and treatments. 2024 Vol. 30 (12): 2043-2048 [Abstract] ( 15 ) HTML (1 KB)  PDF (1264 KB)  ( 12 ) 2049 Relationship between Serum SAA PCT FN RBP and Disease Severity Prognosis in Elderly Patients with Pneumonia WANG Lianghui, MA Di, YIN Jun, et al DOI: 10.3969/j.issn.1006-6233.2024.12.020 Objective: To analyze the relationship between serum amyloid a (SAA), procalcitonin (PCT), plasma fibronectin (FN), retinol binding protein (RBP) and disease severity, prognosis in elderly patients with pneumonia.Methods: A retrospective analysis was performed on the clinical data of 85 elderly patients with pneumonia in the hospital between April 2021 and April 2024. According to prognosis, patients were divided into good prognosis group (60 cases) and poor prognosis group (25 cases). After admission, levels of blood indexes [PCT, SAA, RBP, hemoglobin (Hb), FN, albumin (ALB), interleukin-6 (IL-6), hypersensitive C-reactive protein (hs-CRP), β2 microglobulin], hemocytes [white blood cell count (WBC), neutrophil ratio, lymphocyte ratio, platelets] and blood gas indexes [partial pressure of oxygen (PO2), partial pressure of carbon dioxide (PCO2), bicarbonate ion concentration (HCO3-), base excess (BE), blood lactate concentration (Lac)], and scores of acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) were compared between the two groups. The correlation between blood indexes and APACHE Ⅱ score was analyzed by Spearman correlation analysis. The blood indexes affecting the prognosis of elderly patients with pneumonia were analyzed with Logistic regression analysis. The prognostic value of relevant indexes was analyzed with ROC curves.Results: The levels of PCT, SAA, IL-6, hs-CRP, RBP and β2 microglobulin in the poor prognosis group were higher than those in the good prognosis group, and the levels of ALB and FN were lower than those in the good prognosis group (P<0.05). APACHE Ⅱ score was higher in poor prognosis group (P<0.05). The results of Spearman correlation analysis showed that levels of serum PCT, SAA and RBP were positively correlated with APACHE Ⅱ score, while FN level was negatively correlated with it (P<0.05). The results of Logistic regression analysis showed that serum PCT, SAA, FN and RBP were all influencing factors of prognosis in elderly patients with pneumonia (P<0.05). The results of ROC curves analysis showed that serum PCT (AUC=0.819), SAA (AUC=0.775), FN (AUC=0.792) and RBP (AUC=0.736) were all of good predictive value in prognosis of elderly patients with pneumonia (P<0.05).Conclusion: The higher the levels of serum SAA, PCT and RBP, and the lower the FN level, the severer the disease and the worse the prognosis in elderly patients with pneumonia, and all the above four indexes have good prognostic value for elderly patients with pneumonia. 2024 Vol. 30 (12): 2049-2054 [Abstract] ( 22 ) HTML (1 KB)  PDF (1394 KB)  ( 39 ) 2054 Correlation between NLR IL-6 CRP with the Prognosis of Patients with Pooled Severe Pneumonia and Septic Shock SHI Meng, CAI Xueqin, LI Qiong DOI: 10.3969/j.issn.1006-6233.2024.12.021 Objective: To analyze the correlation neutrophil to lymphocyte ratio (NLR), interleukin-6 (IL-6) and C-reactive protein (CRP) with the prognosis of patients with pooled severe pneumonia (SP) and septic shock (SS). Methods: Ninety- eight patients pooled SP and SS admitted to our hospital from January 2019 to December 2023 were enrolled. The key observation was the correlation of the changes in levels of NLR, IL-6, and CRP with the prognosis of patients. Results: The levels of NLR, IL-6 and CRP in the poor prognosis group were significantly higher than those in the good prognosis group (P<0.05). Pearson correlation analysis revealed that the levels of NLR, IL-6 and CRP were positively correlated with shock index (r=0.412, 0.381, 0.354, P<0.05). Univariate analysis showed that shock index, SOFA score and malnutrition were associated with poor prognosis (P<0.05). Multivariate Logistic regression found that NLR, IL-6, CRP, shock index, SOFA score and malnutrition were risk factors for poor prognosis (P<0.05). ROC analysis showed that the AUCs of NLR, IL-6 and CRP in evaluating the poor prognosis of patients were 0.757, 0.743 and 0.708 respectively. The AUC of NLR+IL-6+CRP was the highest (0.841), and the sensitivity and specificity were 0.774 and 0.836 respectively. The AUC, sensitivity and specificity of combined assessment of NLR, IL-6, CRP, shock index, SOFA score and malnutrition were 0.906, 0.871 and 0.806 respectively.Conclusion: NLR, IL-6 and CRP were abnormally elevated in patients with severe pneumonia combined with septic shock. These three indexes combined with shock index, SOFA score and nutritional status had a high value in evaluating the prognosis of patients. 2024 Vol. 30 (12): 2054-2059 [Abstract] ( 20 ) HTML (1 KB)  PDF (1238 KB)  ( 61 ) 2059 Relationship between β-hCG Levels TNF-α/IL-10 Ratio and Adverse Pregnancy Outcomes in Pregnant Women with Gestational Diabetes Mellitus ZHU Ling, ZENG Lingfang DOI: 10.3969/j.issn.1006-6233.2024.12.022 Objective: To observe the changes of β-human chorionic gonadotropin (β-hCG) and tumor necrosis factor-α (TNF-α)/interleukin-10 (IL-10) ratio in patients with gestational diabetes mellitus (GDM), and to analyze the relationship with adverse pregnancy outcomes.Methods: A total of 102 GDM patients admitted to our hospital from January 2020 to January 2023 were included. The serum levels of β-hCG and the TNF-α/IL-10 ratio were detected, and their association with adverse pregnancy outcomes was analyzed.Results: Serum β-hCG and TNF-α/IL-10 ratio of pregnant women with adverse pregnancy outcomes were significantly higher than those of pregnant women with normal pregnancy outcomes (P<0.05). Univariate analysis showed that history of adverse pregnancy, bacterial vaginosis, age and HbA1c were associated with adverse pregnancy outcomes in GDM patients (P<0.05). Logistic regression analysis indicated that adverse pregnancy history, age, HbA1c, β-hCG, and TNF-α/IL-10 ratio were risk factors for adverse pregnancy outcomes in GDM patients (P<0.05). ROC results suggested that the AUCs of serum β-hCG and TNF-α/IL-10 in evaluating adverse pregnancy outcomes in GDM patients were 0.804 (95%CI: 0.695~0.912) and 0.729 (95%CI: 0.614~0.844) respectively. The AUC of the combination of the two indicators was 0.867 (95%CI: 0.787~0.947) in assessing the adverse pregnancy outcomes, and the sensitivity and specificity under the maximum Youden index were 0.786 and 0.811, respectively.Conclusion: Abnormal increases in serum β-hCG and the TNF-α/IL-10 ratio in GDM pregnant women are associated with adverse pregnancy outcomes, and their combined assessment has a high evaluation value for adverse pregnancy outcomes. 2024 Vol. 30 (12): 2059-2063 [Abstract] ( 21 ) HTML (1 KB)  PDF (1216 KB)  ( 8 ) 2064 Correlation between Serum 25OHD FABP4 and sVEGFR-1 with Disease Progression in Hypertensive Disorder of Pregnancy MENG Xiaohui, YU Yuanyuan, HUANG Rui DOI: 10.3969/j.issn.1006-6233.2024.12.023 Objective: To investigate correlation between serum levels of 25-hydroxyvitamin D (25OHD), fatty acid binding protein 4 (FABP4), and soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) with disease progression of pregnant women with hypertensive disorders of pregnancy (HDP). Methods: From October 2022 to October 2023, 120 pregnant women with HDP in People's Hospital of Xinjiang Uygur Autonomous Region (study group) and 120 normal pregnant women (control group) were included. The patients in the study group were assigned into the gestational hypertension (GH) subgroup (n=79) and the pre-eclampsia (PE) subgroup (n=41) according to the disease progression. At admission, the levels of serum 25OHD, FABP4, and sVEGFR-1 were compared between the study group and the control group, and the levels of serum 25OHD, FABP4, and sVEGFR-1 and the incidence of adverse pregnancy outcome were compared between the two subgroups. The correlation between the levels of serum 25OHD, FABP4, and sVEGFR-1 at admission with the progression of GH to PE was assessed. The levels of serum 25OHD, FABP4, and sVEGFR-1 at admission were compared among patients with different pregnancy outcomes, and the values of the indexes in evaluating the progression of GH to PE and predicting adverse pregnancy outcomes were analyzed. Results: At admission, the study group had significantly lower serum 25OHD level, and higher levels of FABP4 and sVEGFR-1 than the control group (all P<0.05). PE subgroup had significantly lower serum 25OHD level and the incidence of adverse pregnancy outcomes, and higher levels of FABP4 and sVEGFR-1 than the GH subgroup (all P<0.05). Low serum 25OHD levels and high serum FABP4 and sVEGFR-1 levels at admission were independent risk factors for the progression of GH to PE (P<0.05). The total incidence of adverse pregnancy outcomes in the study group was 35.83% (43/120). At admission, the patients with poor pregnancy outcomes presented significantly lower serum 25OHD level, and higher levels of FABP4 and sVEGFR-1 than those with good pregnancy outcomes (P<0.05); the area under the curve (AUC) of the combined indicator in evaluating the progression of GH to PE at admission was significantly higher than that of 25OHD, FABP4, and sVEGFR-1 alone (0.937 vs 0.793, 0.813, 0.835; P<0.05); the AUC of the combined indicator in predicting adverse pregnancy outcomes of HDP patients was significantly higher than that of 25OHD, FABP4, and sVEGFR-1 alone (0.930 vs 0.810, 0.757, 0.772; P<0.05). Conclusion: The decreased serum 25OHD level and the increased serum FABP4 and sVEGFR-1 levels in HDP patients are independent risk factors for the progression of GH to PE. They have certain clinical value in evaluating the progression of GH to PE and predicting the risk of adverse pregnancy outcomes. 2024 Vol. 30 (12): 2064-2068 [Abstract] ( 22 ) HTML (1 KB)  PDF (1352 KB)  ( 5 ) 2069 Predictive Value of Logistic Regression Model Based on Uterine Artery Blood Flow Parameters on Adverse Delivery Outcomes in Patients with Hypertensive Disorder of Pregnancy WANG Min, ZHANG Xiaocai, ZHU Lihong, et al DOI: 10.3969/j.issn.1006-6233.2024.12.024 Objective: To explore the predictive value of Logistic regression model of uterine artery blood flow parameters on the adverse delivery outcomes in patients with hypertensive disorder of pregnancy (HDP).Methods: A total of 203 HDP patients who received prenatal examination in the hospital from April 2022 to June 2023 were selected as the study subjects (HDP group), and 100 pregnant women without abortion history were included in control group. All pregnant women were tested for uterine artery blood flow parameters in the second trimester.The pulsatility index (PI), resistance index (RI), and the ratio of peak systolic velocity to diastolic velocity (S/D) were assessed. Independent predictors of adverse delivery outcomes were identified using logistic regression analysis. The effectiveness of the logistic model in predicting outcomes was evaluated through receiver operating characteristic (ROC) curve analysis.Results: The PI, RI and S/D in HDP group were significantly higher than those in control group (P<0.05). Among the 203 patients with HDP, 135 cases had adverse pregnancy outcomes, with the incidence rate of 66.50%. Logistic regression model showed that PI, RI, S/D ratio were independent risk factors for adverse delivery outcomes in HDP patients (P<0.05). The AUC, SE and 95%CI of logistic regression model based on uterine artery blood flow parameters were 0.952, 0.324 and 0.913-0.977 in predicting the adverse delivery outcomes (P<0.001), and the sensitivity, specificity and Youden indexes were 0.896, 0.956 and 0.852.Conclusion: Uterine artery blood flow parameters are of great significance in predicting the risk of adverse delivery outcomes in patients with HDP, and can be used as an effective tool for clinical risk assessment. 2024 Vol. 30 (12): 2069-2073 [Abstract] ( 20 ) HTML (1 KB)  PDF (1231 KB)  ( 37 ) 2073 Expression of ALK BRAF and Her-2 in Hp-Infected Gastric Cancer Tissues and Their Predictive Value for Poor Prognosis WU Minmin, CHEN Mingguang, LIU Jiying DOI: 10.3969/j.issn.1006-6233.2024.12.025 Objective: To observe the expression of anagenic lymphoma kinase (ALK), murine sarcoma viral oncogenic homology B1 (BRAF) and human epidermal growth factor receptor 2 (Her-2) in gastric cancer infected by Helicobacter pylori (Hp), and to analyze their prediction value of poor prognosis in HP-infected gastric cancer.Methods: A total of 106 patients with Hp-infected gastric cancer admitted to the the First Hospital of Nanping Affiliated to Fujian Medical University from October 2019 to November 2022 were selected to compare the differences in the positive rates of ALK, BRAF, and Her-2 expression in Hp-infected gastric cancer tissues and paracancerous tissues, and to analyze the relationship between the expression of ALK, BRAF, and Her-2 in gastric cancer tissues and the clinical features and pathological manifestations of Hp-infected gastric cancer. After 1 year of follow-up, the patients were divided into good prognosis group (61 cases) and poor prognosis group (45 cases) according to their postoperative recurrence or death, and the ALK, BRAF, Her-2 expression of Hp-infected gastric cancer tissues with different prognosis were compared; the predictive efficacy and early warning value of ALK, BRAF, Her-2 expression of Hp-infected gastric cancer tissues on poor prognosis were analyzed.Results: The positive rates of ALK, BRAF, and Her-2 expression in Hp-infected gastric cancer tissues (17.92%, 67.92%, and 25.47%) were higher than those in adjacent tissues (2.83%, 7.55%, and 4.72%) (χ2=12.984, 82.230, and 17.814, P<0.05). Significant differences were observed in the positive rates of ALK, BRAF, and Her-2 expression in Hp-infected gastric cancer tissues across different levels of tissue differentiation, infiltration, TNM staging, and lymph node metastasis (P<0.05). Among 106 patients with Hp-infected gastric cancer who underwent follow-up, 45 were included in the poor prognosis group (32 relapsed and 13 died), and 61 were included in the good prognosis group. The positive rates of ALK, BRAF, and Her-2 expression in Hp-infected gastric cancer tissues in the poor prognosis group (35.56%, 93.33%, and 46.67%) were higher than those in the good prognosis group (4.92%, 49.18%, and 9.84%) (χ2=16.523, 23.172, and 18.504, P<0.05). The combined assessment of ALK, BRAF, and Her-2 expression in Hp-infected gastric cancer tissues showed the highest AUC for poor prognosis, reaching 0.901 (95%CI: 0.827~0.950) (P<0.05), with a sensitivity of 86.67% and a specificity of 93.44%, and the predictive efficacy was superior to that of ALK, BRAF, and Her-2 expression detection alone. The RR values for poor prognosis caused by ALK, BRAF, and Her-2 positivity in Hp-infected gastric cancer were 2.526 (1.771~3.604), 6.611 (2.205~19.821), and 2.560 (1.733~3.781), respectively (P<0.05).Conclusion: The expression of ALK, BRAF and Her-2 in HP-infected gastric cancer is closely related to the degree of tissue differentiation, invasion, TNM staging, and lymph node metastasis, which has high predictive and early warning value for the adverse prognosis of HP-infected gastric cancer. 2024 Vol. 30 (12): 2073-2078 [Abstract] ( 19 ) HTML (1 KB)  PDF (1275 KB)  ( 11 ) 2078 Value of Combined Serum Estrogen, Sideromodulin, and t-PINP/β-CTX Ratio for Prediction of Fracture Risk in Elderly Women with Osteoporosis WU Xulei, LIU Kun, WANG Chengliang, et al DOI: 10.3969/j.issn.1006-6233.2024.12.026 Objective: To investigate the combined value of serum estrogen (E2), ferriregulation (Hepcidin) and the ratio of total type I collagen amino terminal prolongating peptide (t-PINP)/Type I collagen carboxy-terminal peptide β special sequence (β-CTX) in predicting fracture risk in elderly women with osteoporosis (OP).Methods: A total of 205 elderly female OP patients from January 2021 to June 2023 were selected and divided into fracture group and non-fracture group according to whether they had fractures during the 1-year follow-up period. Venous blood samples were collected from both groups on the admission morning of the patient. The levels of serum E2, Hepcidin, serum t-PINP, and β-CTX were detected using chemiluminescence. Baseline data, serum E2, Hepcidin, and t-PINP/β-CTX ratio levels were compared between the two groups. The predictive value of serum E2, Hepcidin, and t-PINP/β-CTX for fracture risk was analyzed.Results: After a year of follow-up, the contact of 4 patients were lost, and a total of 201 patients completed the follow-up. Among them, 63 cases had fractures and 138 cases had no fractures, with a fracture incidence at 31.34%. Patients with and without fractures were included in the fracture group and non-fracture group, respectively. The age, menopausal years, history of fragility fractures, and history of estrogen use in the fracture group were higher than those in the non-fracture group, while the bone mineral density of the hip, lumbar spine, and Wald triangle area was lower than that in the non-fracture group (P<0.05). The serum E2, Hepcidin, and t-PINP/β-CTX ratios in the fracture group were (25.65±4.63) pg/mL, (76.16±15.82) ng/mL, and (126.50±15.25), respectively, which were lower than those in the non-fracture group (32.71±5.09) pg/mL, (102.43±18.90) ng/mL, and (152.68±20.10). The t-PINP and β-CTX were (60.72±6.57) ng/mL and (0.48±0.10) ng/mL, respectively, which were higher than those in the non-fracture group (56.49±5.03) ng/mL and (0.37±0.08) ng/mL (t=9.378, 9.600, 5.007, 8.342, 9.196, all P<0.001). Serum E2, Hepcidin, and t-PINP/β-CTX ratio were positively correlated with bone mineral density in the hip, lumbar spine, and Wald triangle area (r=0.622, 0.641, 0.658, 0.609, 0.617, 0.634, 0.710, 0.729, 0.746, all P<0.001). Serum E2, Hepcidin and t-PINP/β-CTX ratio were still independent influencing factors for fracture in elderly female OP patients before and after controlling for age, menopause years, fragility fracture history, estrogen use history and other factors (P<0.05). Serum E2, Hepcidin and t-PINP/β-CTX ratios predicted the fracture AUC of elderly female OP patients to be 0.810, 0.789 and 0.760, respectively, and the optimal cut-off values were 27.77 pg/mL, 88.49 ng/mL and 142.52, respectively. Sensitivity was 68.25%, 76.19%, 73.02%, and specificity was 76.81%, 74.64%, 74.64%, respectively. The AUC of fracture predicted by the combination of the three methods was 0.918 (95%CI: 0.871-0.952), the sensitivity was 87.30%, and the specificity was 83.33%, which was significantly better than that predicted by the three methods alone (P<0.05).Conclusion: Serum E2, Hepcidin and t-PINP/β-CTX ratios are significantly correlated with bone mineral density in elderly female OP patients, and are independent influencing factors for fracture occurrence, and the combined value of predicting fracture risk is reliable. 2024 Vol. 30 (12): 2078-2084 [Abstract] ( 20 ) HTML (1 KB)  PDF (1274 KB)  ( 48 ) 2084 Establishment and Verification of a Nomogram Model to Predict the Risk of Postoperative Venous Thromboembolism in Patients with Thoracolumbar Fracture GUAN Yan, XU Wenshuo, FU Ying, et al DOI: 10.3969/j.issn.1006-6233.2024.12.027 Objective: To construct and verify a Nomogram model for predicting the risk of postoperative venous thromboembolism (VTE) in patients with thoracolumbar fracture, and thus providing a reference for prevention and treatment of VTE. Methods: A total of 252 patients with thoracolumbar fracture admitted to our hospital from June 2020 to February 2023 were enrolled. Lower extremity vascular ultrasound examination was performed at 14 days postoperatively. According to the presence and absence of VTE, the patients were divided into VTE group (n=43) and non-VTE group (n=209). The clinical data of the two groups were assessed, and the influencing factors of postoperative VTE in patients with thoracolumbar fracture were analyzed by least absolute shrinkage and selection operator (LASSO)-Logistic regression. A Nomogram prediction model was constructed, and the model was evaluated by calibration curve and receiver operating characteristic (ROC) curve. Results: The ratio of patients ≥60 years old, hyperlipidemia, general anesthesia, lumbar and back fascia injury, bone mineral density ≥ -2.5g /cm2 and body mass index in VTE group were significantly higher than those in non-VTE group, the time from fracture to admission, the time from fracture to operation and the operation time were significantly longer than those in non-VTE group. Hemoglobin (Hb) and albumin (ALB) in VTE group were significantly lower than those in non-VTE group (P<0.05). The regression screening variables by LASSO regression were age, lumbar and back fascia injury, pooled osteoporosis, body mass index, time from fracture to admission, ALB, Hb, and operation time. Age, lumbar and back fascia injury, pooled osteoporosis, body mass index, time from fracture to admission, ALB, operation time, and Hb were factors that influence the incidence of postoperative VTE in patients with thoracolumbar fractures (P<0.05). Based on the results obtained from LASSO-logistic regression, a Nomogram prediction model was constructed. The ROC curve showed that the model had good predictive value for predicting postoperative VTE in patients in both the training and validation sets. The calibration results showed a high consistency degree of agreement between the predicted incidence of postoperative VTE and actual events in both sets. Conclusion: The factors influencing the occurrence of postoperative VTE in patients with thoracolumbar fractures were age, lumbar and back fascia injury, pooled osteoporosis, body mass index, time from fracture to admission, ALB, Hb, and operation time. Based on these factors, a Nomogram prediction model was constructed to predict the occurrence of postoperative VTE with good consistency and efficacy. 2024 Vol. 30 (12): 2084-2091 [Abstract] ( 25 ) HTML (1 KB)  PDF (2197 KB)  ( 12 ) 2091 Association between Serum CTRP3 BGLAP sCD44 Levels with Fracture Healing in Patients with Traumatic Intertrochanteric Femoral Fractures Underwent Proximal Femoral Nail Anti-rotation HE Jinhai, ZOU Guoyou, JI Bingqing, et al DOI: 10.3969/j.issn.1006-6233.2024.12.028 Objective: To observe the serum levels of complement C1q/tumor necrosis factor-related protein 3 (CTRP3), osteocalcin (Bglap) and soluble phagocytic glycoprotein-1 (sCD44) in patients with traumatic intertrochanteric femoral fractures (IFF) underwent proximal femoral nail anti-rotation (PFNA), and to analyze their correlation with fracture healing effect. Methods: A total of 112 patients with traumatic IFF in the hospital from January 2019 to December 2023 were included in the study. The serum levels of CTRP3, BGLAP and sCD44 after PFNA were measured among all patients, and the association between these indicators and fracture healing effect was analyzed. Results: After PFNA treatment, twenty-three cases representing 112 patients had poor healing. The levels of serum CTRP3 and BGLAP in the poor healing group were significantly lower than those in the good healing group, while the sCD44 level was significantly higher (P<0.05). Univariate analysis found that age, smoking and surgical time were related to poor postoperative healing (P<0.05). Logistic regression analysis showed that age, surgical time and sCD44 level were risk factors affecting poor postoperative healing, and all the associations were statistically significant (P<0.05). Serum CTRP3 and BGLAP levels were protective factors for poor healing (P<0.05). Receiver operating characteristic (ROC) curves revealed that the area under the ROC curve (AUC) of CTRP3, BGLAP and sCD44 in evaluating poor healing after PFNA was 0.703, 0.743 and 0.724, respectively, but the AUC of CTRP3+BGLAP+sCD44 in evaluating poor postoperative healing was 0.803, with the sensitivity and specificity under the maximum Youden index of 0.957 and 0.528 respectively. Conclusion: The decreases of serum CTRP3, BGLAP and sCD44 levels have a certain association with poor fracture postoperative healing in patients with traumatic IFF underwent PFNA, and have a high evaluated value on fracture healing effect. 2024 Vol. 30 (12): 2091-2096 [Abstract] ( 19 ) HTML (1 KB)  PDF (1247 KB)  ( 5 ) 2096 Clinical Study on the Value of 3.0T MRI in Assessing Neurological Impairment and Hemorrhagic Transformation in Acute Cerebral Infarction ZHANG Nan, SHI Zhichao, WANG Jing, et al DOI: 10.3969/j.issn.1006-6233.2024.12.029 Objective: To explore the value of 3.0T magnetic resonance imaging (MRI) in assessing neurological injury in acute cerebral infarction (ACI) and to analyze its predictive value for hemorrhagic transformation (HT), providing a reference for clinical improvement of prognosis.Methods: A total of 104 ACI patients admitted to our hospital from January 2022 to March 2023 were selected. All subjects underwent 3.0T MRI examination of the head. According to the NIHSS score, the degree of neurological impairment was divided into three subgroups: mild group (<7 points, 34 cases), moderate group (7-14 points, 44 cases), and severe group (>14 points, 26 cases). Additionally, based on the results of the 3.0T MRI examination 7-10 days after onset, the patients were divided into two subgroups: HT group (31 cases) and non-HT group (73 cases). The 3.0T MRI parameters of ACI patients in different subgroups were compared, and their relationship with neurological impairment and HT was analyzed to further assess the degree of neurological impairment and predict the risk of HT.Results: The VRA and FA in the mild group (0.15±0.03, 0.30±0.08) were greater than in the moderate group (0.13±0.03, 0.24±0.06), which in turn were greater than in the severe group (0.06±0.02, 0.12±0.03) (F=82.774, 63.120, P<0.05); the Vp, Kep, Ve, and ktrans in the mild group (0.19±0.06, 0.57±0.17, 0.29±0.08, 0.43±0.13) were less than in the moderate group (0.32±0.09, 0.78±0.22, 0.47±0.14, 0.69±0.18), which in turn were less than in the severe group (0.45±0.11, 0.93±0.24, 0.75±0.17, 0.83±0.20) (F=65.855, 22.384, 88.892, 43.605, P<0.05); the VRA and FA in the HT group (0.08±0.02, 0.19±0.06) were lower than in the non-HT group (0.13±0.03, 0.31±0.09) (t=9.972, 7.978, P<0.05); the Vp, Kep, Ve, and ktrans in the HT group (0.38±0.11, 0.85±0.25, 0.56±0.14, 0.72±0.21) were higher than in the non-HT group (0.16±0.05, 0.52±0.16, 0.28±0.08, 0.44±0.21) (t=14.065, 8.063, 12.881, 6.220, P<0.05); the 3.0T MRI parameters VRA and FA were negatively correlated with neurological impairment and HT in ACI patients (r1=-0.641, -0.627, r2=-0.625, -0.611, P<0.05), while Vp, Kep, Ve, and ktrans were positively correlated with neurological impairment and HT in ACI patients (r1=0.701, 0.579, 0.754, 0.688, r2=0.673, 0.589, 0.670, 0.583, P<0.05); the combined prediction of VRA, FA, Vp, Kep, Ve, and ktrans for severe neurological impairment had the largest AUC of 0.913 (95%CI: 0.841-0.959), with a sensitivity of 84.62% and specificity of 87.18% (P<0.05), and the combined prediction of these 3.0T MRI parameters for the occurrence of HT in ACI patients had the largest AUC of 0.925 (95%CI: 0.857-0.968), with a sensitivity of 90.32% and specificity of 79.45% (P<0.05).Conclusion: The 3.0T MRI examination parameters are closely related to neurological impairment and HT in ACI patients, and the combination of VRA, FA, Vp, Kep, Ve, and ktrans has a high predictive value for severe neurological impairment and HT occurrence, which is of clinical guidance significance. 2024 Vol. 30 (12): 2096-2102 [Abstract] ( 17 ) HTML (1 KB)  PDF (1743 KB)  ( 89 ) 2102 Correlation of Serum Uric Acid SHG Ratio and TyG Index with Occurrence of MACE in Patients with AMI WU Chao, HU Xuejun, LIU Bing, et al DOI: 10.3969/j.issn.1006-6233.2024.12.030 Objective: To analyze the correlation between serum uric acid, stress hyperglycemia (SHG) ratio, triglyceride-glucose (TyG) index, and occurrence of major adverse cardiovascular events (MACE) in patients with acute myocardial infarction (AMI).Methods: The clinical data of 234 patients with AMI in Xuancheng People' s Hospital were retrospectively analyzed from November 2019 to November 2023. According to the occurrence of MACE, the patients were divided into AMI group (patients without MACE, n=182) and MACE group (patients with MACE, n=52). The clinical data and laboratory detection indicators were compared between groups of patients, and the risk factors for MACE in AMI were analyzed. ROC curve was used to evaluate the predictive efficiency of serum uric acid, SHG ratio and TyG index on MACE in AMI patients.Results: Time from infarction to reperfusion, serum uric acid, SHG ratio, TyG index, Scr, cTnT and LVEF were all risk factors for MACE in AMI patients (P<0.05). The curves of serum uric acid, SHG ratio and TyG index all had certain predictive value on MACE in AMI patients (AUC>0.5), and their sensitivities were 86.54%, 76.92% and 67.31%, the specificities were 45.60%, 67.58% and 67.58%, and the cut-off values were 337.31 μmol/L, 1.16 and 4.21 respectively.Conclusion: Serum uric acid, SHG ratio, and TyG index have a certain correlation with the occurrence of MACE in AMI patients. The higher the above indicators, the greater the risk of MACE in patients. Clinically, serum uric acid, SHG ratio and TyG index can be used as predictive indicators of postoperative MACE in AMI patients in order to take early targeted preventive measures and improve the prognosis of patients as much as possible. 2024 Vol. 30 (12): 2102-2106 [Abstract] ( 30 ) HTML (1 KB)  PDF (1186 KB)  ( 57 ) 2102 Correlation of Serum Uric Acid SHG Ratio and TyG Index with Occurrence of MACE in Patients with AMI WU Chao, HU Xuejun, LIU Bing, et al DOI: 10.3969/j.issn.1006-6233.2024.12.030 Objective: To analyze the correlation between serum uric acid, stress hyperglycemia (SHG) ratio, triglyceride-glucose (TyG) index, and occurrence of major adverse cardiovascular events (MACE) in patients with acute myocardial infarction (AMI).Methods: The clinical data of 234 patients with AMI in Xuancheng People' s Hospital were retrospectively analyzed from November 2019 to November 2023. According to the occurrence of MACE, the patients were divided into AMI group (patients without MACE, n=182) and MACE group (patients with MACE, n=52). The clinical data and laboratory detection indicators were compared between groups of patients, and the risk factors for MACE in AMI were analyzed. ROC curve was used to evaluate the predictive efficiency of serum uric acid, SHG ratio and TyG index on MACE in AMI patients.Results: Time from infarction to reperfusion, serum uric acid, SHG ratio, TyG index, Scr, cTnT and LVEF were all risk factors for MACE in AMI patients (P<0.05). The curves of serum uric acid, SHG ratio and TyG index all had certain predictive value on MACE in AMI patients (AUC>0.5), and their sensitivities were 86.54%, 76.92% and 67.31%, the specificities were 45.60%, 67.58% and 67.58%, and the cut-off values were 337.31 μmol/L, 1.16 and 4.21 respectively.Conclusion: Serum uric acid, SHG ratio, and TyG index have a certain correlation with the occurrence of MACE in AMI patients. The higher the above indicators, the greater the risk of MACE in patients. Clinically, serum uric acid, SHG ratio and TyG index can be used as predictive indicators of postoperative MACE in AMI patients in order to take early targeted preventive measures and improve the prognosis of patients as much as possible. 2024 Vol. 30 (12): 2102-2106 [Abstract] ( 7 ) HTML (1 KB)  PDF (1186 KB)  ( 108 ) 2107 Relationship between Serum Levels of CIRBP and Notch1 with Disease Severity and Prognosis in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease GAO Lihua, LI Yannan, YU Ling, et al DOI: 10.3969/j.issn.1006-6233.2024.12.031 Objective: To investigate the relationship between serum levels of cold-inducible RNA-binding protein (CIRBP) and notch receptor 1 (Notch1) with disease severity and prognosis in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods: A total of 157 AECOPD patients (AECOPD group), 70 stable COPD patients (COPD stability group), and 70 healthy volunteers (control group) treated at our hospital from February 2023 to January 2024 were included. According to disease severity, the AECOPD patients were categorized into a mild AECOPD group (60 cases), moderate AECOPD group (55 cases), and severe AECOPD group (42 cases). Based on 6-month follow-up outcomes, they were further divided into a poor prognosis group (50 cases) and a good prognosis group (107 cases). Serum levels of CIRBP and Notch1 were measured by enzyme-linked immunosorbent assay, and the receiver operating characteristic curve(ROC) was drawn to evaluate their predictive efficacy for prognosis. Results: Serum levels of CIRBP and Notch1 progressively increased across the control, COPD stability, and AECOPD groups, with significant differences (F=172.36, 104.024, all P<0.001). Similarly, CIRBP and Notch1 levels rose significantly across the mild, moderate, and severe AECOPD groups (F=64.686, 54.409, all P<0.05). At the 6-month follow-up, the incidence of poor prognosis in 157 AECOPD patients was 31.85% (50/157). CIRBP and Notch1 levels were significantly higher in the poor prognosis group than in the good prognosis group (t=7.261, 6.956, all P<0.05). Independent risk factors for poor prognosis in AECOPD patients included increased disease severity (OR=4.012, 95%CI: 1.996 to 8.067), elevated CIRBP (OR=1.012, 95%CI: 1.005 to 1.020), and elevated Notch1 (OR=1.014, 95%CI: 1.007 to 1.022) (all P<0.05). The combined prediction using serum CIRBP and Notch1 levels yielded an area under the curve (AUC) of 0.871 for poor prognosis in AECOPD patients, which was significantly greater than the AUCs for CIRBP (0.793) or Notch1 (0.798) alone (Z=2.833, 2.696, all P<0.05). Conclusion: Elevated levels of serum CIRBP and Notch1 in AECOPD patients were associated with increased disease severity and poor prognostic turnover, and the combination of serum CIRBP and Notch1 levels had high energy efficiency in predicting poor prognostic turnover in AECOPD patients. 2024 Vol. 30 (12): 2107-2112 [Abstract] ( 30 ) HTML (1 KB)  PDF (1297 KB)  ( 137 )

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