Abstract：Objective: To explore the effect of the Bushen Huoxue Recipe on the ultrastructure of the ovaries in rats with Diminished Ovarian Reserve (DOR). Methods: The DOR rat model was established with intraperitoneal injection of cyclophosphamide, 50mg/kg on the first day and 4mg/kg once a day on second day to 14th day. The rats were divided into blank control group, model group, Bushen Huoxue Recipe high, medium and low dose groups, and western medicine Conjugated Estrogens Tablets as positive controls, with 10 rats in each group. The behavioral changes of the rats were observed during administration, and the ultrastructure of the ovary was observed under electron microscope after the last administration. Results: Compared with the treatment groups, the rats in the model group showed decreased activity, arched backs, curled up postures, disheveled and shedding fur, irritability, and weight loss. Under transmission electron microscope, the ovarian tissues of rats in the model group showed dilated and vacuolated rough endoplasmic reticulum, ribosomes detached from the membrane, nuclear pyknosis, widened perinuclear space, obvious mitochondrial swelling, broken and disappeared cristae, and blurred membrane fusion. In the high and medium dose groups of Bushen Huoxue Recipe and the Conjugated estrogen tablet group, the nuclei of granulosa cells in the follicles were relatively large, nuclear pyknosis was not obvious, the perinuclear space was widened in some cases, and the mitochondrial morphology and structure were basically normal or showed mild mitochondrial swelling, partial disappearance of cristae, and partial blurred membranes. In the low-dose group, dilated and vacuolated rough endoplasmic reticulum, ribosomes detached from the membrane, nuclear pyknosis, more vacuolated mitochondria, mitochondrial swelling, broken and disappeared cristae, and disrupted membrane structure were observed, and autophagosomes were visible. Conclusion: The ultrastructure of ovary in DOR rats was disturbed, the apoptosis of follicular granulosa cells was obvious, and the mitochondria were damaged. Bushen Huoxue Recipe in medium and high dose and Conjugated estrogen tablet group has obviously improved effect, and the effect of Bushen Huoxue Recipe shows a certain dose-effect relationship.
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2025, Vol. 31 
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