基于RhoA/ROCK1通路探讨花旗松素对缺氧/复氧诱导的心肌细胞损伤的影响

doi:10.3969/j.issn.1006-6233.2026.02.010

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摘要 目的: 基于RhoA/ROCK1通路,探讨花旗松素(TAX)对缺氧/复氧(H/R)诱导的心肌细胞损伤的影响。方法: CCK-8筛选TAX处理H/R诱导H9C2的最佳浓度;H9C2细胞分为正常组、H/R组、H/R+TAX组、H/R+ROCK抑制剂(Y-27632)组、H/R+TAX+RhoA激活剂溶血磷脂酸(LPA)组。台盼蓝染色检测各组H9C2细胞存活率;试剂盒检测各组H9C2细胞LDH释放率;流式细胞仪和TUENL染色检测各组H9C2细胞凋亡率;DCFH-DA法和ELISA检测各组H9C2细胞氧化应激和炎症反应;Western blot检测各组H9C2细胞RhoA/ROCK1通路相关蛋白表达。结果: 与对照组比较,H/R组H9C2细胞存活率明显降低(P<0.05);与H/R组比较,H/R+TAX 20μmoL/L组和H/R+TAX 40μmoL/L组H9C2细胞存活率明显升高(P<0.05);与H/R+TAX 40μmoL/L组比较,H/R+TAX 80μmoL/L组H9C2细胞存活率明显降低(P<0.05)。与对照组比较,H/R组H9C2细胞存活率、GSH-Px下调,LDH释放率、凋亡率以及TUENL阳性细胞数、ROS荧光强度、MDA、NOX4、IL-6、TNF-α、NLRP3、ICAM-1、RhoA、ROCK1、p-MLC2/MLC2上调(P<0.05);与H/R组比较,H/R+TAX组和H/R+Y-27632组H9C2细胞存活率、GSH-Px上调,LDH释放率、凋亡率以及TUENL阳性细胞数、ROS荧光强度、MDA、NOX4、IL-6、TNF-α、NLRP3、ICAM-1、ROCK1、p-MLC2/MLC2下调(P<0.05);与H/R+TAX组比较,H/R+TAX+LPA组H9C2细胞存活率、GSH-Px下调,LDH释放率、凋亡率以及TUENL阳性细胞数、ROS荧光强度、MDA、NOX4、IL-6、TNF-α、NLRP3、ICAM-1、RhoA、ROCK1、p-MLC2/MLC2上调(P<0.05)。结论: TAX通过抑制RhoA/ROCK1通路调控氧化应激、炎症反应和细胞凋亡,缓解H/R诱导的心肌细胞损伤。

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	徐颖
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关键词 ：花旗松素, 缺氧/复氧, 心肌细胞, RhoA/ROCK1通路

Abstract：Objective: To explore the effect of taxifolin (TAX) on hypoxia/reoxygenation (H/R)-induced myocardial cell injury based on the RhoA/ROCK1 pathway. Methods: CCK-8 was used to screen for the optimal concentration of TAX treatment for H/R induced H9C2. H9C2 cells were separated into normal group, H/R group, H/R+TAX group, H/R+ROCK inhibitor (Y-27632) group, and H/R+TAX+RhoA activator lysophosphatidic acid (LPA) group. Trypan blue staining was performed to detect the survival rate of H9C2 cells. The reagent kit was used to detect the LDH release rate of H9C2 cells. Flow cytometry and TUNEL staining were performed to detect the apoptosis rate of H9C2 cells in each group. DCFH-DA method and ELISA were performed to detect oxidative stress and inflammatory response in H9C2 cells. Western blot was used to detect the expression of RhoA/ROCK1 pathway related proteins in H9C2 cells of each group. Results: Compared with the control group, the survival rate of H9C2 cells in the H/R group was prominently lower (P<0.05). Compared with the H/R group, the survival rate of H9C2 cells was prominently higher in the H/R+TAX 20μmol/L group and the H/R+TAX 40μmol/L group (P<0.05). Compared with the H/R+TAX 40μmol/L group, the H/R+TAX 80μmol/L group showed a prominent decrease in H9C2 cell survival rate (P<0.05). Compared with the control group, the H/R group showed downregulation of H9C2 cell survival rate and GSH-Px, and upregulation of LDH release rate, apoptosis rate, TUNEL positive cell number, ROS fluorescence intensity, MDA, NOX4, IL-6, TNF-α, NLRP3, ICAM-1, RhoA, ROCK1, and p-MLC2/MLC2 (P<0.05). Compared with the H/R group, the H/R+TAX group and H/R+Y-27632 group showed upregulation of H9C2 cell survival rate and GSH-Px, and downregulation of LDH release rate, apoptosis rate, TUNEL positive cell number, ROS fluorescence intensity, MDA, NOX4, IL-6, TNF-α, NLRP3, ICAM-1, ROCK1, and p-MLC2/MLC2 (P<0.05). Compared with the H/R+TAX group, the H/R+TAX+LPA group showed downregulation of H9C2 cell survival rate and GSH-Px, and upregulation of LDH release rate, apoptosis rate, TUNEL positive cell number, ROS fluorescence intensity, MDA, NOX4, IL-6, TNF-α, NLRP3, ICAM-1, RhoA, ROCK1, and p-MLC2/MLC2 (P<0.05). Conclusion: TAX alleviates H/R-induced myocardial cell injury by inhibiting the RhoA/ROCK1 pathway to regulate oxidative stress, inflammatory response, and cell apoptosis.

Key words： Taxifolin Hypoxia/reoxygenation Myocardial cells RhoA/ROCK1 pathway

基金资助:河北省保定市科技计划项目,(编号:2441ZF209)

通讯作者: 张宁

引用本文:

徐颖, 李丽华, 刘晨, 张宁. 基于RhoA/ROCK1通路探讨花旗松素对缺氧/复氧诱导的心肌细胞损伤的影响[J]. 河北医学, 2026, 32(2): 240-247.
XU Ying, LI Lihua, LIU Chen, et al. Effect of Taxifolin on Hypoxia/Reoxygenation-Induced Myocardial Cell Injury Based on the RhoA/ROCK1 Pathway. HeBei Med, 2026, 32(2): 240-247.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2026.02.010 或 http://www.hbyxzzs.cn/CN/Y2026/V32/I2/240

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