MiR-125a-5p靶向FGFR1和FGFR3抑制宫颈癌细胞恶性生物学行为的机制研究

doi:10.3969/j.issn.1006-6233.2024.09.02

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Abstract Objective: To investigate the mechanism by which microRNA-125a-5p (miR-125a-5p) targets fibroblast growth factor receptor (FGFR) 1 and FGFR3 to inhibit the malignant biological behavior of cervical cancer (CC) cells.Methods: The qRT-PCR method was used to detect the expression of miR-125a-5p, FGFR1, and FGFR3 in CC tissue samples and adjacent tissue samples of 37 CC patients who underwent surgery in our hospital from June 2022 to June 2023. CC CaSKi cells were randomly separated into control group, NC-mimics group, miR-125a-5p-mimics group, miR-125a-5p mimics+pcDNA-NC group, miR-125a-5p mimics+pcDNA-FGFR1 group, and miR-125a-5p mimics+pcDNA-FGFR3 group. The expression of miR-125a-5p, FGFR1, and FGFR3 in each group was measured. MTT and plate cloning methods were applied to detect CaSKi cell proliferation. Transwell experiment was conducted to detect the migration and invasion of CaSKi cells. Flow cytometry was used to detect apoptosis of CaSKi cells. WB was applied to detect the expression of FGFR1 and FGFR3 proteins in CaSKi cells. A dual luciferase reporter gene experiment was applied to verify the relationship between miR-125a-5p and FGFR1, and between miR-125a-5p and FGFR3.Results: The expression of miR-125a-5p in CC tissue was lower than that in adjacent cancer tissue, while the expression of FGFR1 and FGFR3 was higher than that in adjacent cancer tissue (P<0.05). The apoptosis rate and miR-125a-5p expression in CaSKi cells in the miR-125a-5p-mimics group were higher than those in the control group and NC-mimics group, the EdU positive cell rate, OD₄₉₀, migration number, invasion number, FGFR1 mRNA and protein, and FGFR3 mRNA and protein expression were lower than those in the control group and NC-mimics group (P<0.05). Compared with the miR-125a-5p-mimics group and the miR-125a-5p mimics+pcDNA-NC group, the apoptosis rate of the miR-125a-5p mimics+pcDNA-FGFR1 group decreased, the EdU positive cell rate, OD₄₉₀, migration number, invasion number, FGFR1 mRNA and protein expression increased (P<0.05), the apoptosis rate of the miR-125a-5p mimics+pcDNA-FGFR3 group decreased, while the EdU positive cell rate, OD₄₉₀, migration number, invasion number, FGFR3 mRNA and protein expression increased (P<0.05). MiR-125a-5p targeted negative regulation of FGFR1 and FGFR3.Conclusion: Overexpression of miR-125a-5p can inhibit the malignant biological behavior of CC cells, and its mechanism may be achieved by targeting FGFR1 and FGFR3.

Key words： Micro RNA-125a-5p Fibroblast growth factor receptor 1 Fibroblast growth factor receptor 3 Cervical cancer Cell malignant biological behavior

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.09.02 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I9/1421

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