红花苷联合miR-214对肺癌细胞增殖及凋亡的作用

doi:10.3969/j.issn.1006-6233.2024.12.01

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Abstract Objective: To investigate the effects of carthamin combined with miR-214 on the proliferation and apoptosis of lung cancer cells.Methods: Quantitative real-time PCR was conducted to determine miR-214 expression in human normal lung epithelial cells BEAS-2B and lung cancer cells A549 and H1299. miR-214 mimic (miR-214) and negative control (miR-NC) were transfected into A549 and H1299 cells. A549 and H1299 cells were divided into control, carthamin, miR-214 and carthamin +miR-214 groups. Cells in carthamin group were treated with 80μmoL/L carthamin, cells in miR-214 group were transfected with miR-214, and cells in carthamin+miR-214 group were co-treated with carthamin and miR-214. The cells without any treatment were used as control group. CCK-8, EdU and flow cytometry assays were performed to evaluate cell viability, proliferation, cell cycle and apoptosis, respectively. The protein expressions were analyzed with Western blot. A xenograft tumor model was used to evaluate the synergy of carthamin and miR-214 on lung cancer growth in vivo.Results: Compared with BEAS-2B cells, miR-214 expression was significantly reduced in A549 and H1299 cells. miR-214-transfected A549 and H1299 cells expressed higher level of miR-214, compared to miR-NC. Compared with control group, cell viability, EdU-positive cells and S-phase cells were markedly reduced, while G0/G1-phase cells and apoptotic cells were substantially increased in carthamin and miR-214 groups; the cell viability, EdU-positive cells and S-phase cells were less, while the G0/G1-phase cells and apoptotic cells were more in carthamin+miR-214 group than those in carthamin and miR-214 groups. Compared with control group, the phosphorylation of Akt and PI3K and protein expressions of Bcl-2, Cyclin D1 and CDK4 were considerably downregulated, while the expressions of Bax and cl-Caspase-3 were significantly up-regulated in carthamin and miR-214 groups. Akt and PI3K phosphorylations and protein levels of Bcl-2, Cyclin D1 and CDK4 in carthamin +miR-214 group were much lower, while Bax and cl-Caspase-3 expressions were higher than those in carthamin and miR-214 groups. In vivo experiments showed that compared with control group, the tumor volume and weight of mice were significantly reduced in carthamin and miR-214 groups, which were further smaller in carthamin+miR-214 group than those in carthamin and miR-214 groups.Conclusion: Carthamin combined with miR-214 could inhibit the proliferation but promote apoptosis of lung cancer cells in vitro and in vivo, probably through the inactivation of PI3K/Akt signaling pathway.

Key words： Lung cancer Carthamin miR-214 Proliferation Apoptosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.12.01 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I12/1937

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