基于PI3K/AKT/mTOR通路探讨白藜芦醇对UVB诱导的小鼠皮肤光老化损伤的影响

doi:10.3969/j.issn.1006-6233.2025.04.05

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Abstract Objective: To investigate the effect of resveratrol (RES) on ultraviolet B (UVB) induced skin photoaging damage in mice by regulating the phosphoinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. Methods: Mice were assigned into control group,model group,PI3K inhibitor group (LY294002 group),resveratrol group (RES),and resveratrol+PI3K activator group (RES+740 Y-P),with 12 mice in each group; Except for the Control group,mice in all other groups were exposed to UVB radiation.The skin moisture content was measured,oxidative stress (GSH-Px,SOD,MDA) and inflammation (IL-6,TNF-α) related indicators in the skin tissue were measured using ELISA method.The morphological changes in the skin tissue were measured using HE staining,and the collagen fiber content in the skin tissue was measured using Masson staining.The autophagy of skin tissue cells was observed using transmission electron microscopy,the expression of AQP3 and Claudin-1 in skin tissue was measured using immunohistochemistry,and the expression of autophagy (Atg7,LC3-Ⅱ/LC3-I,p62) and PI3K/AKT/mTOR pathway related proteins in the skin tissue was measured using Western blot. Results: Compared with the Control group,the skin moisture content,skin tissue GSH-Px,SOD activities,collagen content,autophagosome count,AQP3,Claudin-1,LC3-Ⅱ/LC3-I,and Atg7 protein levels were lower in the Model group,while the MDA and IL-6,TNF-α levels,epidermal thickness,p62 protein level,the phosphorylation level of signaling pathway protein were higher (P<0.05).Compared with the Model group,the skin moisture content,skin tissue GSH-Px,SOD activities,collagen content,autophagosome count,AQP3,Claudin-1,LC3-Ⅱ/LC3-I,and Atg7 protein levels were higher in the LY294002 group and RES group,while the MDA and IL-6,TNF-α levels,epidermal thickness,p62 protein level,the phosphorylation level of signaling pathway protein were lower (P<0.05).Compared with the RES group,the skin moisture content,skin tissue GSH-Px,SOD activities,collagen content,autophagosome count,AQP3,Claudin-1,LC3-Ⅱ/LC3-I,and Atg7 protein levels were lower in the RES+740 Y-P group.In contrast,the MDA and IL-6,TNF-α levels,epidermal thickness,p62 protein level,the phosphorylation level of signaling pathway protein were higher (P<0.05). Conclusion: RES may improve skin photoaging damage in UVB-irradiated mice by inhibiting the activation of the PI3K/AKT/mTOR signaling pathway,activating autophagy,and reducing oxidative stress and inflammatory response.

Key words： Resveratrol Ultraviolet B Skin photoaging Autophagy PI3K/AKT/mTOR pathway

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.04.05 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I4/556

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