宫颈癌组织中miR-214-3p THBS2的表达及临床意义

doi:10.3969/j.issn.1006-6233.2025.07.017

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1441 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the expression of microRNA-214-3p (miR-214-3p) and thrombospondin 2 (THBS2) in cervical cancer tissues and their relationship with pathological parameters and prognosis. Methods: A total of 84 cervical cancer patients who underwent total hysterectomy or radical hysterectomy in our hospital from January 2019 to March 2021 were enrolled. The expression levels of miR-214-3p and THBS2 mRNA in cervical cancer and adjacent tissues were detected by real-time quantitative PCR. THBS2 protein expression was assessed by immunohistochemistry. The binding sites between miR-214-3p and THBS2 were predicted using the TargetScan database, and Pearson correlation analysis was performed to determine the correlation between miR-214-3p and THBS2 expression in cervical cancer tissues. Based on the median expression levels, cervical cancer tissues were divided into high/low miR-214-3p and THBS2 mRNA expression groups. Kaplan-Meier survival curves were plotted to evaluate progression-free survival (PFS) in cervical cancer patients with high/low miR-214-3p and THBS2 mRNA expression. Multivariate Cox regression analysis was performed to identify factors influencing patient prognosis, using the prognosis of cervical cancer patients as the dependent variable. Results: The expression level of miR-214-3p was significantly lower in cervical cancer tissues (0.99±0.13) than in adjacent tissues (1.55±0.18), while THBS2 mRNA expression was higher in cancer tissues (1.13±0.07) compared to adjacent tissues (0.78±0.12) (t=-22.522 and 23.443, all P<0.001). A binding site was identified at positions 3934-3941 in the 3′ untranslated region of THBS2 for miR-214-3p. A negative correlation was observed between miR-214-3p and THBS2 expression in cervical cancer tissues (r=-0.830, P<0.001). The expression of miR-214-3p and THBS2 mRNA was significantly associated with tumor differentiation, FIGO stage, and lymph node metastasis (all P<0.05). During a 3-year follow-up, the progression-free survival rate was 65.48% (55/84). Patients with high miR-214-3p expression had a higher 3-year PFS rate, while those with high THBS2 mRNA expression had a lower 3-year PFS rate (Log-rank χ²=14.120 and 10.350, all P<0.05). Independent risk factors for death in cervical cancer patients included poor differentiation (HR=4.532, 95% CI: 1.230~16.707), FIGO stage III (HR=6.038, 95% CI: 1.712~21.290), lymph node metastasis (HR=9.004, 95% CI: 2.167~37.422), and THBS2 mRNA ≥1.11 (HR=6.521, 95% CI: 1.474~28.841). High miR-214-3p expression (≥1.02) was an independent protective factor (H=0.141, 95% CI: 0.035~0.568) (all P<0.05). Conclusion: Low expression of miR-214-3p and high expression of THBS2 mRNA in cervical cancer tissues are associated with unfavorable pathological characteristics and poor prognosis.

Key words： Cervical cancer MicroRNA-214-3p Thrombospondin 2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	YAN Qiongqiong
	LI Yaping
	ZHU Haijun
	et al

Cite this article:

YAN Qiongqiong,LI Yaping,ZHU Haijun, et al. Expression and Clinical Significance of miR-214-3p and THBS2 in Cervical Cancer Tissues[J]. HeBei Med, 2025, 31(7): 1152-1158.

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.07.017 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I7/1152

[1] 郑荣寿,陈茹,韩冰峰,等.2022年中国恶性肿瘤流行情况分析[J].中华肿瘤杂志,2024,46(3):221-231.
[2] 马守叶,刘会玲,魏振宏,等.微小RNA在宫颈癌诊断和治疗耐药作用中的研究进展[J].江苏医药,2023,49(6):624-628.
[3] Zhou Y,Wang Y,Lin M,et al.LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells[J].Cancer Cell Int,2021,21(1):400.
[4] 马郏俊延,芮天楠,张永胜.血小板反应蛋白2在恶性肿瘤中作用的研究进展[J].基础医学与临床,2023,43(12):1881-1885.
[5] Wang X,Xu J,Hua F,et al.MiR-214-3p suppresses cervical cancer cell metastasis by downregulating THBS2[J].Cell Mol Biol (Noisy-le-grand),2023,69(9):195-200.
[6] 中国抗癌协会肿瘤内分泌专业委员会,周琦.妇科恶性肿瘤免疫治疗中国专家共识(2023年版)[J].中国癌症杂志,2023,33(10):954-968.
[7] Chen C,Zhang Q,Wang B,et al.SPTBN2 regulated by miR-214-3p inhibits the proliferation and migration of colorectal cancer cells[J].Cell Mol Biol (Noisy-le-grand),2023,69(14):126-131.
[8] 周大新,谢方利,刘海科,等.miR-214-3p通过靶向调节p53增强胃癌细胞的转移能力[J].安徽医科大学学报,2021,56(5):769-773.
[9] Peng Q,Wang L,Zuo L,et al.HPV E6/E7:insights into their regulatory role and mechanism in signaling pathways in HPV-associated tumor[J].Cancer Gene Ther,2024,31(1):9-17.
[10] 黎康玲,张潇迪,刘珏,等.骨髓间充质干细胞外泌体源性miR-214-3p调控IKBKB基因促进宫颈癌细胞焦亡[J].中国现代医生,2023,61(25):50-55.
[11] 王语晴.HPV16病毒编码circE7促进宫颈癌细胞增殖迁移、抑制铁死亡的机制研究[D].湖北:武汉大学,2022.
[12] 王佳敏.THBS2通过PI3K/AKT信号通路影响胃癌恶性生物学行为的研究[D].辽宁:中国医科大学,2024.
[13] 刘利,李颖,孙文白,等.血清淀粉样蛋白A1通过抑制血小板反应蛋白4表达促进胰腺导管腺癌细胞的增殖和转移[J].中华实验外科杂志,2024,41(3):504-507.
[14] 刘琴,吴丹,胡华,等.PI3K/Akt信号通路与女性相关恶性肿瘤关系的研究进展[J].中南医学科学杂志,2021,49(6):738-740.