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| Effects of Ginsenoside Rg1 Regulating SIRT1/AMPK/PGC1α Signaling Pathway on Adriamycin-Induced Myocardial Injury in Rats |
| XIE Yuemin, HAN Aizi, CHEN Qian, et al |
| Handan Central Hospital, Hebei Handan 056001, China |
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Abstract Objective: To investigate the protective effect of ginsenoside Rg1 (G-Rg1) on adriamycin-induced myocardial injury in rats. Methods: A total of 75 SPF grade SD male rats were randomly divided into control group, model group, low, high dose G-Rg1 group and high-dose G-Rg1+SIRT1 inhibitor EX-527 group, with 15 rats in each group. The rat myocardial injury model was constructed by intraperitoneal injection of adriamycin. The rats in the low and high dose G-Rg1 groups were given 25 and 50mg/kg G-Rg1, respectively; the rats in the high dose G-Rg1+SIRT1 inhibitor EX-527 group were given 50mg/kg G-Rg1 by gavage and injected with 5μg/kg EX-527 in the tail vein; the control group and the model group were given gavage and intraperitoneally injected with the same amount of normal saline. The cardiac function of rats were detected; the levels of lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), oxidative stress indicator superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of myocardial tissue in rats were observed by hematoxylin-eosin (HE) staining. Apoptosis in rat myocardial tissue was detected by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling (TUNEL) staining. The contents of nicotinamide adenine dinucleotide (NAD+), adenosine monophosphate (AMP) and adenosine triphosphate (ATP) in myocardial tissue were determined by high performance liquid chromatography. The expression of silencing information regulator 1 (SIRT1)/adenylate activated protein kinase (AMPK)/peroxisome proliferators-activated receptor γ coactivator α (PGC1α) pathway-related proteins was detected by Western-Blot. Results: High dose G-Rg1 significantly improve the pathological damage, such as myocardial cell hypertrophy, swelling and deformation, inflammatory cell infiltration (P<0.05). Increased left ventricular ejection fraction, left ventricular shortening fraction, serum SOD level, myocardial NAD+ and ATP, SIRT1, PGC1α protein expression and AMPK phosphorylation level, and decreased left ventricular end diastolic diameter, left ventricular end-systolic diameter, serum LDH , CK , CK-MB, MDA levels, AMP, AMP/ATP and myocardial cell apoptosis rate in adriamycin-induced myocardial injury rats (P<0.05). However, all the above improvement effects of G-Rg1 at high dose were mitigated or inhibited by EX-527 (P<0.05). Conclusion: G-Rg1 may play a role in alleviating adriamycin-induced myocardial injury in rats by activating the SIRT1/AMPK/PGC1α signaling pathway.
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2025, Vol. 31 
