杨梅素调节Wnt/β-catenin信号通路对腰椎间盘突出症大鼠椎间盘退变的影响

doi:10.3969/j.issn.1006-6233.2024.10.09

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Abstract Objective: To investigate the effect of myricetin (MYR) on intervertebral disc degeneration (IVDD) in rats with lumbar disc herniation (LDH) by regulating the Wnt/β-catenin signaling pathway. Methods: Rats were randomly divided into four groups: Sham group, LDH group, MYR group, and MYR+LiCl group (Wnt/β-catenin signaling pathway activator), with 12 rats in each group. Except for the Sham group, an LDH rat model was replicated using autologous nucleus pulposus transplantation. After successful model establishment, drug intervention was performed once daily for 28 days. Von Frey hair filaments and radiant heat pain tests were used to analyze the pain sensitization behavior of rats. ELISA was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in serum. HE staining was used to observe the pathological changes of intervertebral disc tissue. TUNEL staining was used to determine the apoptosis of nucleus pulposus cells. Immunohistochemistry was used to detect the expression of matrix metalloproteinase 13 (MMP13) in rat intervertebral disc tissue. Western blot was used to detect the protein expression of the Wnt/β-catenin signaling pathway. Results: Compared with the Sham group, rats in the LDH group showed shrinkage and irregular arrangement of nucleus pulposus cells, reduced cell number, and annulus fibrosus rupture. MWT and TWL were decreased, while TNF-α, IL-1β levels, disc tissue pathological score, nucleus pulposus cell apoptosis rate, MMP13, Wnt3a and β-catenin expression were increased (P<0.05). Compared with the LDH group, MYR group showed significant improvement in nucleus pulposus cell morphology and annulus fibrosus structure, increased MWT and TWL, and decreased TNF-α, IL-1β levels, disc tissue pathological score, nucleus pulposus cell apoptosis rate, MMP13, Wnt3a, and β-catenin expression (P<0.05). Wnt/β-catenin signaling pathway activator LiCl increased serum levels of inflammatory factors, promoted nucleus pulposus cell apoptosis, aggravated disc tissue pathological damage, and weakened the delaying effect of MYR on IVDD in LDH rats (P<0.05). Conclusion: Myricetin may improve intervertebral disc degeneration in LDH rats by inhibiting the activation of the Wnt/β-catenin signaling pathway, reducing inflammation, and decreasing nucleus pulposus cell apoptosis.

Key words： Myricetin Wnt/β-catenin signaling pathway Lumbar disc herniation Intervertebral disc degeneration

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.10.09 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I10/1634

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