1α,25-二羟维生素D3通过调控TGF-β<sub>1</sub>诱导的EMT抑制子宫内膜癌细胞的迁移和侵袭

doi:10.3969/j.issn.1006-6233.2025.03.013

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Abstract Objective: To explore the role of 1α,25-dihydroxyvitamin D3 [1,25(OH)₂D₃] in the regulation of epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β₁ (TGF-β₁) in endometrial cancer (EC). Methods: HEC-1B cells were cultured in vitro and treated with different concentrations (0, 25 nM, 50 nM, 100 nM, 200 nM, 500 nM) of 1,25(OH)₂D₃. Based on the results of the CCK-8 assay to assess cell viability, 100 nM 1,25(OH)₂D₃ was selected for subsequent experiments. The experimental groups were as follows: control group (Ctrl), TGF-β₁ group, 1,25(OH)₂D₃ group, TGF-β₁ + 1,25(OH)₂D₃ group, TGF-β₁ + TGF-β₁ inhibitor P144 diammonium group (TGF-β₁ + P144). Cell morphological changes were observed under an optical microscope, and Western blotting was used to detect the protein expression of E-cadherin, N-cadherin, Vimentin, and TGF-β₁. The migration ability of the cells was assessed by the scratch assay, and the invasion ability was assessed by the Transwell assay. Results: Compared with the Ctrl group, cells in the TGF-β₁ group changed from typical cobblestone-like morphology to spindle-shaped mesenchymal cell morphology, with significantly reduced cell-cell adhesion and a more dispersed arrangement. Meanwhile, E-cadherin expression was significantly reduced (P<0.05), and the expression of N-cadherin, Vimentin, and TGF-β₁, as well as migration distance and invasion cell count, were significantly increased (P<0.05). Compared with the Ctrl group, the 1,25(OH)₂D₃ group showed significantly higher E-cadherin expression (P<0.05), and significantly lower expression of N-cadherin, Vimentin, and TGF-β₁, as well as migration distance and invasion cell count (P<0.05). Compared with the TGF-β₁ group, both the TGF-β₁ + 1,25(OH)₂D₃ group and the TGF-β₁ + P144 group exhibited significantly higher E-cadherin expression (P<0.05), and significantly lower expression of N-cadherin, Vimentin, and TGF-β₁, as well as migration distance and invasion cell count (P<0.05). Conclusion: 1,25(OH)₂D₃ can inhibit the migration and invasion of HEC-1B cells by regulating TGF-β₁-induced EMT.

Key words： 1α,25-Dihydroxyvitamin D3 TGF-β₁ Epithelial-mesenchymal transition (EMT) Endometrial cancer Migration Invasion

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.03.013 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I3/425

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