LncRNA NEAT1靶向调控miR-125b-5p对高糖诱导人视网膜血管内皮细胞凋亡迁移的影响

doi:10.3969/j.issn.1006-6233.2025.09.013

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Abstract Objective: To investigate the impacts of long non-coding RNA (LncRNA) nuclear-enriched abundant transeript 1 (NEAT1) on high glucose (HG) induced apoptosis and migration of human retinal endothelial cells (RMECs) through targeted regulation of microRNA-125b-5p (miR-125b-5p). Methods: RMECs were assigned into Control group (normal culture), HG group (30mmol/L glucose induction for 24 hours), sh-NC group (HG+transfection of sh-NC), sh-NEAT1 group (HG+transfection of sh-NEAT1), sh-NEAT1+inhibitor-NC group (HG+transfection of sh-NEAT1+inhibitor-NC), and sh-NEAT1+miR-125b-5p inhibitor group (HG+transfection of sh-NEAT1+miR-125b-5p inhibitor). QRT-PCR was used to detect the expression of LncRNA NEAT1 and miR-125b-5p of RMECs in each group. MTT assay, scratch assay, and flow cytometry were used to detect the proliferation, migration, and apoptosis of RMECs, respectively. ELISA method was used to detect the expression of TNF - α, IL-6, and IL-8 in the supernatant of RMEC. Western blot was used to detect the expression of MMP-9 and Cleaved Caspase-3 proteins in RMECs. Results: The LncRNA NEAT1, apoptosis rate, TNF - α, IL-6, IL-8, and Cleaved Caspase-3 in HG group were higher than those in Control group, while miR-125b-5p, A490 value, scratch healing rate, and MMP-9 were lower (P<0.05). The LncRNA NEAT1, apoptosis rate, TNF - α, IL-6, IL-8, and Cleaved Caspase-3 in sh-NEAT1 group were lower than those in HG and sh-NC groups, while miR-125b-5p, A490 value, scratch healing rate, and MMP-9 were higher (P<0.05). The apoptosis rate, TNF-α, IL-6, IL-8, and Cleaved Caspase-3 in sh-NEAT1+miR-125b-5p inhibitor group were higher than those in sh-NEAT1 group and sh-NEAT1+inhibitor-NC group, while miR-125b-5p, A490 value, scratch healing rate, and MMP-9 were lower (P<0.05). LncRNA NEAT1 could target negative regulation of miR-125b-5p. Conclusion: Silencing LncRNA NEAT1 can promote the proliferation and migration of RMECs, inhibit cell apoptosis, and it may be achieved by targeted regulation of miR-125b-5p.

Key words： Long non-coding RNA Nuclear-enriched abundant transeript 1 MicroRNA-125b-5p Human retinal endothelial cells Apoptosis Migration

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	CAI Chenghang
	ZHANG Jin

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CAI Chenghang,ZHANG Jin. Impacts of LncRNA NEAT1 on High Glucose Induced Apoptosis and Migration of Human Retinal Endothelial Cells through Targeted Regulation of Mir-125b-5p[J]. HeBei Med, 2025, 31(9): 1487-1493.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.09.013 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I9/1487

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