山姜素调节LncRNA FTX/miR-335-5p轴对前列腺癌细胞增殖及侵袭的影响

doi:10.3969/j.issn.1006-6233.2025.09.09

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1757 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To explore the effects of alpinetin (ALP) on the proliferation and invasion of prostate cancer (PC) cells by regulating LncRNA FTX/miR-335-5p axis. Methods: The human prostate cancer cell line LNCaP was cultured in vitro, and the MTT colorimetric method was used to detect the proliferative activity of ALP on LNCaP cells. LNCaP cells were assigned into NC group, ALP group, ALP+pcDNA3.1-NC group, and ALP+pcDNA3.1-FTX group. CCK-8 and clone formation experiments were used to detect the proliferation ability of LNCaP cells in each group. Transwell method was used to detect the invasion ability of LNCaP cells in each group. qRT-PCR was used to detect LncRNA FTX and miR-335-5p of LNCaP cells in various groups. Western blot experiment was performed to detect cell proliferation and invasion related proteins. Dual luciferase reporter gene assay was performed to detect the targeting relationship between LncRNA FTX and miR-335-5p. Construct a nude mouse PC transplant tumor model, detect the mass and volume of tumors in each group, and measure the expression levels of LncRNA FTX and miR-335-5p in tumor tissues of each group. Results: For the NC group, the ALP group and ALP+pcDNA3.1-NC group had conspicuously lower LNCaP cell survival rate, clone formation number, cell invasion number, LncRNA FTX, cyclin D1 (CCND1), myelocytomatosis viral oncogene homolog (MYC), matrix metalloproteinase-2 (MMP) -2 and MMP-9 mRNAs and proteins (P<0.05), and conspicuously higher miR-335-5p (P<0.05). For the ALP+pcDNA3.1-NC group, the ALP+pcDNA3.1-FTX group had conspicuously higher LNCaP cell survival rate, clone formation number, cell invasion number, LncRNA FTX, cyclin D1 (CCND1), myelocytomatosis viral oncogene homolog (MYC), matrix metalloproteinase-2 (MMP) -2 and MMP-9 mRNAs and proteins (P<0.05), and conspicuously lower miR-335-5p (P<0.05). The dual luciferase reporter gene experiment showed that LncRNA FTX targeted negative regulation of miR-335-5p (P<0.05). The results of the nude mouse tumorigenesis experiment showed that compared with the Model group, the ALP group had significantly smaller tumor mass and volume, and the expression of LncRNA FTX in tumor tissue was significantly downregulated, while the expression of miR-335-5p was significantly up-regulated (P<0.05). Conclusion: ALP may inhibit proliferation and invasion of PC cells by regulating LncRNA FTX/miR-335-5p axis.

Key words： Prostate cancer Alpinetin LncRNA FTX miR-335-5p Proliferation Invasion

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LEI Xiao
	BI Genwang
	JIA Lan
	et al

Cite this article:

LEI Xiao,BI Genwang,JIA Lan, et al. Effects of Alpinetin on the Proliferation and Invasion of Prostate Cancer Cells by Regulating LncRNA FTX/miR-335-5p Axis[J]. HeBei Med, 2025, 31(9): 1458-1465.

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.09.09 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I9/1458

[1] Conteduca V,Mosca A,Brighi N,et al.New prognostic biomarkers in metastatic castration-resistant prostate cancer[J].Cells,2021,10(1):193.
[2] Mumuni S,O'donnell C, Doody O.The risk factors and screening uptake for prostate cancer:a scoping review[J].Healthcare(Basel),2023,11(20):2780.
[3] Deluce J E,Cardenas L,Lalani A K,et al.Emerging biomarker-guided therapies in prostate cancer[J].Curr Oncol,2022,29(7):5054-5076.
[4] Zhang F,Wang X S,Tang B,et al.Long non-coding RNA FTX promotes gastric cancer progression by targeting miR-215[J].Eur Rev Med Pharmacol Sci,2020,24(6):3037-3048.
[5] Du W,Tang H,Lei Z,et al.miR-335-5p inhibits TGF-β1-induced epithelial-mesenchymal transition in non-small cell lung cancer via ROCK1[J].Respir Res,2019,20(1):225.
[6] Takayama K I.Splicing factors have an essential role in prostate cancer progression and androgen receptor signaling[J].Biomolecules,2019,9(4):131.
[7] Sekhoacha M,Riet K,Motloung P,et al.Prostate cancer review:genetics,diagnosis,treatment options,and alternative approaches[J].Molecules,2022,27(17):5730.
[8] Zhao X,Guo X,Shen J,et al.Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling[J].Mol Med Rep,2018,18(4):4030-4036.
[9] Jang H,Ham J,Song J,Song G,Lim W.Alpinumisoflavone impairs mitochondrial respiration via oxidative stress and MAPK/PI3K regulation in hepatocellular carcinoma cells[J].Antioxidants (Basel),2022,11(10):1929.
[10] Liu Z,Dou C,Yao B,et al.Ftx non coding RNA-derived miR-545 promotes cell proliferation by targeting RIG-I in hepatocellular carcinoma[J].Oncotarget.2016;7(18):25350-25365.
[11] 刘军,杜恒,马彬.LncRNA FTX通过靶向miR-590调控膀胱癌细胞的增殖、迁移和侵袭[J].局解手术学杂志,2024,33(7):630-636.
[12] Liang H,Zhang C,Guan H,et al.LncRNA DANCR promotes cervical cancer progression by upregulating ROCK1 via sponging miR-335-5p[J].Cell Physiol,2019,234(5):7266-7278.
[13] Jia Q,Ye L,Xu S,et al.Circular RNA 0007255 regulates the progression of breast cancer through miR-335-5p/SIX2 axis[J].Thorac Cancer,2020,11(3):619-630.