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2025 Vol. 31, No. 12 Published: 2025-12-31 1937 Effect of miR-98-5p on Inflammatory Response of Allergic Rhinitis Mice by Regulating STAT3 WU Xiongying, et al DOI: 10.3969/j.issn.1006-6233.2025.12.01 Objective: To explore the effect of microRNA-98-5p (miR-98-5p) on the inflammatory response of allergic rhinitis (AR) mice by regulating the signal transducer and activator of transcription 3 (STAT3). Methods: The dual luciferase reporter gene assay was used to determine the targeting relationship between miR-98-5p and STAT3. An AR mouse model induced by ovalbumin (OVA) was constructed, and successfully modeled mice were randomly assigned into AR group, NC agomir group (tail vein injection of NC agomir), miR-98-5p agomir group (tail vein injection of miR-98-5p agomir), and Garcinone D (tail vein injection of miR-98-5p agomir+1mg/kg STAT3 activator Garcinone D), each consisting of 10mice. Another 10mice were included as the control group, and equal amounts of physiological saline were injected into the control group and AR group, respectively. The mice in each group were evaluated for AR symptoms. ELISA was used to measure IgE and inflammatory cytokines in mouse serum. The number of inflammatory cells in nasal lavage fluid (NALF) was detected. The qRT-PCR method was performed to detect miR-98-5p and STAT3 mRNA in the nasal mucosal tissue of AR mice. HE staining was performed to measure the pathology of nasal mucosal tissue. TUNEL staining was performed to measure apoptosis of nasal mucosal tissue cells. Western blot was used to detect STAT3 and Cleaved Caspase-3 proteins in nasal mucosal tissue. Results: MiR-98-5p could target negative regulation of STAT3. The control group had intact nasal mucosal epithelial structure. The nasal mucosal epithelial tissue of the AR group and NC agomir group showed obvious defects, with enlarged intercellular spaces and abundant infiltration of inflammatory cells. The miR-98-5p agomir group showed obvious improvement in nasal mucosal injury, with a small amount of inflammatory cell infiltration visible. Garcinone D group further aggravated nasal mucosal damage and increased inflammatory cell infiltration. The AR group had higher AR symptom scores, lymphocytes, macrophages, eosinophils, neutrophils, apoptosis rate, IgE, IL-4, IL-5, IL-13, STAT3 mRNA and protein, Cleaved Caspase-3, and lower miR-98-5p and IFN-γ than the control group (P<0.05). The miR-98-5p agomir group had lower AR symptom scores, lymphocytes, macrophages, eosinophils, neutrophils, apoptosis rate, IgE, IL-4, IL-5, IL-13, STAT3 mRNA and protein, Cleaved Caspase-3, and higher miR-98-5p and IFN-γ than the AR group and NC agomir group (P<0.05). The Garcinone D group had higher AR symptom scores, lymphocytes, macrophages, eosinophils, neutrophils, apoptosis rate, IgE, IL-4, IL-5, IL-13, STAT3 mRNA and protein, Cleaved Caspase-3, and lower IFN-γ than the miR-98-5p agomir group (P<0.05). Conclusion: MiR-98-5p may inhibit the inflammatory response in AR mice by regulating STAT3. 2025 Vol. 31 (12): 1937-1943 [Abstract] ( 168 ) HTML (1 KB)  PDF (1960 KB)  ( 24 ) 1943 Effect of Dezocine on the Proliferation Invasion and Apoptosis of Endometrial Carcinoma Cells via Regulating p38MAPK/JNK Signaling Pathway LI Chuanjin, CAO Xiaoxia, DING Zhangjun DOI: 10.3969/j.issn.1006-6233.2025.12.02 Objective: To explore the effects of dezocine (Dez) on the proliferation, apoptosis, and epithelial-mesenchymal transition of uterine corpus endometrial cancer (UCEC) cells by regulating p38 mitogen-activated protein kinase 38 (p38MAPK)/c-Jun N-terminal kinase (JNK) signaling pathway. Methods: Human UCEC cells HEC-1A were cultured in vitro and randomly assigned into NC group, low-dose Dez group, medium-dose Dez group, high-dose Dez group (2.5, 5, 10μg/mL Dez), and high-dose Dez+SB203580 group (10μg/mL Dez+10μmol/L p38MAPK inhibitor SB203580). MTS method was used to detect the proliferation of HEC-1A cells. The plate cloning method was used to measure the clonogenic ability of HEC-1A cells. Flow cytometry was used to measure apoptosis of HEC-1A cells. Transwell experiment was used to detect the invasion of HEC-1A cells. Western blot was used to measure the E-cadherin, N-cadherin, Snail, and p38MAPK/JNK signaling pathway proteins. Results: For the NC group, the low-dose, medium-dose, and high-dose Dez groups showed a decrease in D(λ)490nm, colony formation rate, cell invasion number, N-cadherin, and Snail proteins in HEC-1A cells, and an increase in apoptosis rate, E-cadherin, p-p38MAPK/p38MAPK, and p-JNK/JNK proteins (P<0.05). For the high-dose Dez group, the high-dose Dez+SB203580 group showed an increase in D(λ)490nm, colony formation rate, cell invasion number, N-cadherin, and Snail proteins in HEC-1A cells, and a decrease in apoptosis rate, E-cadherin, p-p38MAPK/p38MAPK, and p-JNK/JNK proteins (P<0.05). Conclusion: Dez exerts inhibitory effects on the proliferation, invasion, and epithelial mesenchymal transition of UCEC cells, while promoting UCEC cells apoptosis. This is likely mediated through activating the p38MAPK/JNK signaling pathway. 2025 Vol. 31 (12): 1943-1949 [Abstract] ( 163 ) HTML (1 KB)  PDF (2491 KB)  ( 19 ) 1949 Effect of Amygdalin Regulating cGAS-STING Pathway on Hyperglucose-Induced Human Retinal Endothelial Cell Injury WANG Qian, CHEN Jing, et al DOI: 10.3969/j.issn.1006-6233.2025.12.03 Objective: To investigate the protective effect and molecular mechanism of amygdalin on hyperglucose-induced injury in human retinal endothelial cells (HRECs) through regulation of the cGAS-STING pathway. Methods: An HREC injury model was established using 33 mM high glucose induction. The model was validated through CCK-8 assay, Annexin V/PI double staining, transmission electron microscopy, and ROS level detection to assess cell proliferation, apoptosis, mitochondrial morphology, and intracellular ROS levels. HRECs were treated with 100μM amygdalin to intervene in hyperglucose-induced injury. Changes in cell survival, IFN-α secretion, and cGAS/STING mRNA expression were detected using Annexin V/PI double staining, ELISA, and RT-qPCR. Subsequently, the STING inhibitor C176 (2μM) and cGAS agonist RU.521 (10μM) were applied, and the signaling pathway affected by amygdalin was examined through RT-qPCR, immunofluorescence, Western blot, and Transwell assays. The cGAS gene was knocked down using siRNA, and the alternative TLR4/MyD88 pathway was excluded using the TLR4 inhibitor TAK-242. Results: Treatment with 33 mM high glucose for 48 hours reduced cell viability, increased apoptosis, induced mitochondrial damage, elevated ROS levels, upregulated Cleaved caspase-3 expression, and increased the Bax/Bcl-2 protein expression ratio (all P<0.05). Treatment with 100μM amygdalin significantly improved cell survival, restored LDH levels, upregulated cGAS/STING mRNA expression, promoted STING Golgi translocation, and enhanced IFN-α secretion (all P<0.05). Amygdalin activated the cGAS-STING pathway, leading to increased cell viability, elevated p-TBK1/p-IRF3 expression, and reduced apoptosis -- effects that were reversed by C176 (all P<0.05). Functional recovery experiments showed that amygdalin restored trans-epithelial electrical resistance (TEER) values, increased cell junction density, reduced secretion of inflammatory factors IL-6 and TNF-α, and decreased NO levels (all P<0.05). Knocking down cGAS with siRNA increased apoptosis and SA-β-gal positivity again, while no significant changes were observed in TLR4 pathway-related indicators. Conclusion: Amygdalin specifically activates the cGAS-STING pathway, inhibits mitochondrial damage and DNA breakage, and reduces inflammatory responses, thereby ameliorating high glucose-induced injury in retinal endothelial cells. This effect is independent of the TLR4/MyD88 signaling axis, providing a new therapeutic target for diabetic retinopathy. 2025 Vol. 31 (12): 1949-1955 [Abstract] ( 144 ) HTML (1 KB)  PDF (2193 KB)  ( 13 ) 1955 Effect of miR-93-5p on Neurological Dysfunction in Rats with Cerebral Hemorrhage by Adjusting TXNIP/NLRP3 Pathway AN Shi'en, ZHAO Huan, ZHOU Tao, et al DOI: 10.3969/j.issn.1006-6233.2025.12.04 Objective: To investigate the impacts of miR-93-5p on neurological dysfunction and thioredoxin-interacting protein/nucleotide-binding oligomerization domain-like receptor protein 3 (TXNIP/NLRP3) pathway in rats with cerebral hemorrhage (ICH). Methods: An ICH rat model was constructed, and successfully modeled rats were randomly grouped into model group (ICH group), miR-NC group, miR-93-5p mimics group, miR-93-5p mimics+pcDNA3.1 group, and miR-93-5p mimics+pcDNA-TXNIP group, each with 18 rats. Additionally, 18 normal healthy rats served as control group. Longa rating was conducted. ELISA was used to test serum inflammatory factors. HE staining was used to observe the pathological morphology of brain tissue. TUNEL staining was performed to test neuronal apoptosis. Immunohistochemistry was performed to test Iba1. Western blot was used to detect TXNIP/NLRP3 pathway related proteins. The dual luciferase assay report was used to test the targeting relationship between miR-93-5p and TXNIP. Results: For control group, ICH group revealed clear structural damages to brain tissue, degeneration and necrosis of cells, loose and disordered arrangement, nuclear shrinkage and deep staining, conspicuous interstitial edema, and inflammatory cell infiltration, and the Longa score, brain tissue water content, IL-1β, IL-18, apoptosis rate, Bax, C-caspase-9, C-caspase-3, Iba1, TXNIP, NLRP3, ASC, and C-caspase-1 elevated (P<0.05). The miR-93-5p mimics group showed less pathological damage to brain tissue than the miR-NC group, and the Longa score, brain tissue water content, IL-1β, IL-18, apoptosis rate, Bax, C-caspase-9, C-caspase-3, Iba1, TXNIP, NLRP3, ASC, and C-caspase-1 reduced (P<0.05). The miR-93-5p mimics+pcDNA3.1 group showed more severe brain tissue pathological damage than the miR-93-5p mimics+pcDNA-TXNIP group, and the Longa score, brain tissue water content, IL-1β, IL-18, apoptosis rate, Bax, C-caspase-9, C-caspase-3, Iba1, TXNIP, NLRP3, ASC, and C-caspase-1 elevated (P<0.05). In addition, Starbase website predictions and dual luciferase assay reports indicated a targeted relationship between miR-93-5p and TXNIP. Conclusion: MiR-93-5p can exert a protective effect on neurological damage in ICH rats, which is related to the inhibition of TXNIP/NLRP3 pathway. 2025 Vol. 31 (12): 1955-1963 [Abstract] ( 157 ) HTML (1 KB)  PDF (2635 KB)  ( 11 ) 1963 Effects of Xanthohumol on Neuronal Apoptosis and Inflammatory Response in Rats with Acute Cerebral Infarction by Regulating CaMKK2/AMPK Signaling Pathway CHANG Liang, LI Ming, LI Jing, et al DOI: 10.3969/j.issn.1006-6233.2025.12.05 Objective: To explore the effects of xanthohumol on neuronal apoptosis and inflammatory response in rats with acute cerebral infarction by regulating calcium/calmodulin dependent protein kinase 2 (CaMKK2)/AMP-activated protein kinase (AMPK) signaling pathway. Methods: In this study, an acute cerebral infarction model was established by occlusion of the middle cerebral artery, and was assigned into the cerebral infarction group, the low-dose (L) xanthohumol group (25mg/kg), the high-dose (H) xanthohumol group (50mg/kg), and the H-xanthohumol+inhibitor group, with 12 rats in each. The medication was administered for 28days. Another 12 healthy rats were made the normal group. Longa scoring method was performed to evaluate the neurological function of rats. TTC staining was performed to measure the volume of cerebral infarction. HE and TUNEL staining were used to observe brain tissue lesions and cell apoptosis in rats. ELISA was used to detect the inflammatory factors interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), and IL-6 in rat brain homogenates. Western blot was performed to detect the CaMKK2 and AMPK related proteins. Results: For the normal group, the cerebral infarction group showed improved neurological function scores, cerebral infarction, increased cell apoptosis rate, IL-1β, TNF-α, IL-6, and decreased p-CaMKK2 and p-AMPK (P<0.05). For the cerebral infarction group, the L-xanthohumol group and H-xanthohumol group showed decreased neurological function scores, reduced cerebral infarction volume, reduced brain tissue damage, decreased cell apoptosis rate, IL-1β, TNF-α, IL-6, and increased p-CaMKK2 and p-AMPK (P<0.05), and the therapeutic effect of H-xanthohumol was better than that of L-xanthohumol (P<0.05). The CaMKK2 inhibitor STO-609 eliminated the inhibitory effects of xanthohumol on neuronal apoptosis and inflammatory response in rats with acute cerebral infarction (P<0.05). Conclusion: Xanthohumol may alleviate neuronal apoptosis and inflammatory response in rats with acute cerebral infarction by activating CaMKK2/AMPK signaling pathway. 2025 Vol. 31 (12): 1963-1969 [Abstract] ( 155 ) HTML (1 KB)  PDF (2265 KB)  ( 15 ) 1969 Regulation of TLR4/NF-κB Pathway by Yiqi Huayu Formula Ameliorates Endometritis in Rats by Mediating Cellular Focal Death LU Lixia, LIN Xiaohua, YANG Dandan DOI: 10.3969/j.issn.1006-6233.2025.12.06 Objective: To explore the regulation of the Toll-like receptor 4 / nuclear factor κB (TLR 4 / NF- κ B) pathway-mediated cell pyroptosis to improve endometritis in rats. Methods: Fifty-five SPF-grade rats were selected as the experimental subjects and divided into five groups of 11 rats each by random number table method, which were grouped into 11 blank groups, model group, traditional Chinese medicine control group, Yiqi Huayuquan group, and Yiqi Huayuquan+TAK242 group, each with 10 rats, after being processed by endometritis modeling. The levels of inflammatory factors, oxidative stress, pathological scores and relative expressions of TLR4 and NF-κB were compared among the rats in each group. Results: Compared with the blank group, the model group, the Chinese medicine control group, and the Yiqi Huayuquan+TAK242 group showed increases in pathological composite scores, uterine index, MDA, MPO, IL-8, IL-6, IL-2, IL-1β, Caspase-1, GSDMD levels, IκBα, TLR4, and NF-κBp65 proteins, and decreases in LgM, LgG, IgA, and SOD levels. Compared with the model group, IL-8, IL-6, IL-2, IL-1β levels and pathological composite scores, uterine index, MPO, MDA, Caspase-1, GSDMD levels, IκBα, TLR4, NF-κBp65 protein were decreased, and LgM, LgG, IgA, SOD were elevated in the traditional Chinese medicine control group and Yiqi Huayuquan group. Compared with the group of Yiqi Huayu Fang + TAK242, the pathology composite score, uterine index and the levels of IL-8, IL-6, IL-2, IL-1β, caspase-1, GSDMD, IκBα, TLR4, NF-κBp65, MDA and MPO proteins were increased significantly, and the levels of LgM, LgG, IgA and SOD were decreased significantly, with a statistically significant difference (P<0.05). Conclusion: Yiqi Huayu Fang can exert therapeutic effects by inhibiting the TLR4/NF-κB pathway to attenuate cellular pyroptosis, regulating the activity of inflammatory factors, and improving oxidative stress and immune function. 2025 Vol. 31 (12): 1969-1676 [Abstract] ( 152 ) HTML (1 KB)  PDF (1930 KB)  ( 9 ) 1976 Study on the Ameliorative Effect of Massage to Regulate BDNF/Trkb/p38/JNK Pathway on Rats with Myofascial Pain Syndrome WU Sumin, YU Renyu, SHEN Chongqing, et al DOI: 10.3969/j.issn.1006-6233.2025.12.07 Objective: To investigate the study of the ameliorative effect of nudging to regulate the BDNF/Trkb/p38/JNK pathway on rats with myofascial pain syndrome.Methods: 40 SPF grade rats were selected as experimental subjects and divided into 4 groups of 10 rats each by random number table method, 30 were selected to perform modeling of myofascial pain syndrome, a total of 3 failed, and the remaining 27 were grouped into 10 blank groups (normal feeding, no modeling and treatment), 9 model groups (normal feeding, modeling, no treatment), 9 nudging groups (modeling, normal feeding, and the trigger point localized by 0.5kg of force press), 9 nudging + K-252a group (normal rearing, modeling, intervention trigger point localized at 0.5kg of force press + 500nmol/L concentration of K-252a). After the final intervention in each group, 4ml of blood was collected from the abdominal aorta, and spinal cord tissue from the L4-6 lumbar enlargement segment was harvested. Serum inflammatory factors and stress markers in the abdominal aorta blood were detected using ELISA. Expression of the cell marker OX-42 in spinal cord tissue was assessed via immunohistochemistry. Western Blot analysis was performed to detect levels of BDNF, Trkb, p38, JNK proteins in the L4-6 lumbar bulging segment spinal cord tissue. Results: Compared with the blank group, the model group, massage group, and massage + K-252a group exhibited statistically significant increases in spontaneous activity frequency, IL-8, IL-6, MDA, GSH, β-endorphin levels, and OX-42, BDNF, Trkb, p38, and JNK protein expression (P<0.05). SOD, substance P, and IL-10 levels decreased, while thermal withdrawal latency shortened, with statistically significant differences (P<0.05); Compared with the model group, the massage group exhibited statistically significant reductions in P-substance, MDA, GSH, spontaneous action potential frequency, IL-8, and IL-6 levels, as well as OX-42, BDNF, Trkb, p38, and JNK protein expression levels were significantly lower in the massage group than in the model group (P<0.05). Conversely, levels of β-endorphin, SOD, heat-withdrawal latency, and IL-10 were significantly elevated in the massage group (P<0.05); Compared with the massage group, the massage plus K-252a group showed significantly lower levels of SOD, IL-10, β-endorphin, and heat-withdrawal paw latency, with statistically significant differences (P<0.05). Levels of IL-8, spontaneous action potential frequency, IL-6, MDA, GSH, substance P, OX-42 expression, BDNF, Trkb, p38, JNK protein expression levels were significantly increased, with statistically significant differences (P < 0.05).Conclusion: Massage therapy can effectively alleviate pain in rat models of myofascial pain syndrome. Its mechanism of action may be related to regulating the BDNF/Trkb/p38/JNK signaling pathway, inhibiting the expression of OX-42 in the spinal cord, reducing inflammatory responses, and improving oxidative stress conditions. 2025 Vol. 31 (12): 1976-1983 [Abstract] ( 140 ) HTML (1 KB)  PDF (2542 KB)  ( 8 ) 1983 Effects of Acupuncture Combined with Huangqi Jianzhong Decoction to Regulate PI3K/AKT Signaling Pathway on Epidermal Growth Factor and Gastric Mucosa Function in Rats with Gastric Ulcer WU Jiulong, et al DOI: 10.3969/j.issn.1006-6233.2025.12.08 Objective: To explore the effect of acupuncture combined with Huangqi Jianzhong decoction on improving epidermal growth factor (EGF) and gastric mucosal function in rats with gastric ulcer (GU), and to explore its mechanism based on phosphatidylinositol 3- kinase (PI3K)/ protein kinase B(AKT) signaling pathway. Methods: GU rat model was established by intragastric glacial acetic acid. Rats were randomly divided into control group (normal rats), model group, Huangqi Jianzhong decoction group (6.8g/kg Huangqi Jianzhong decoction by gavage), electroacupuncture group (electroacupuncture stimulating Zusanli and Zhongwan points) and combined group (6.8g/kg Huangqi Jianzhong decoction by gavage and acupuncture stimulating Zusanli and Zhongwan points), with 15 rats in each group. After 2 weeks of treatment, the gastric ulcer area and ulcer index of rats in each group were detected. HE matoxylin eosin (He) staining was used to observe the pathological changes of gastric mucosa. Serum GAStrin (gas), growth hormone inhibin (SS), motilin (MTL), tumor necrosis factor α(TNF-α), interleukin 6(IL-6), prostaglandin E2(PGE2) and epidermal growth factor (EGF) were detected by ELISA kit. Western Blot was used to detect the expression level of PI3K/AKT pathway related proteins. Results: Compared with the control group, the model group had gastric mucosa ulcer, fewer glands, and a large number of inflammatory cells infiltrated, ulcer area and ulcer index were significantly increased, while, SS, PGE2, EGF, p-PI3K and p-Akt levels were significantly decreased (P<0.05), and the levels of GAS, MTL, TNF-α and IL-6 were significantly increased (P<0.05). Compared with model group, the gastric mucosal injury of rats in Huangqi Jianzhong Decoction group, acupuncture group and combined group was significantly improved, the ulcer area and ulcer index were significantly decreased, SS, PGE2, EGF, p-PI3K and p-Akt levels were significantly increased (P<0.05), and the levels of GAS, MTL, TNF-α and IL-6 were significantly decreased (P<0.05). Compared with Huangqi Jianzhong Decoction group and acupuncture group, the ulcer area and ulcer index were significantly decreased, SS, PGE2, EGF, p-PI3K and p-Akt levels of rats in combined group were significantly increased (P<0.05), while the levels of GAS, MTL, TNF-α and IL-6 were significantly decreased (P<0.05). Conclusion: Huangqi Jianzhong decoction combined with acupuncture can promote the repair and healing of gastric mucosa and improve the gastric mucosal function in GU rats by inhibiting inflammatory reaction, and the mechanism involved may be related to the activation of PI3K/AKT signaling pathway. 2025 Vol. 31 (12): 1983-1989 [Abstract] ( 153 ) HTML (1 KB)  PDF (1968 KB)  ( 6 ) 1989 Mechanism Study on the Inhibition of Myocardial Fibrosis in AS Model Rats through cAMP/PKA/PPARγ Signalling Pathway by the Traditional Chinese Medicine Ciwujia GAO Jing, LIU Yang, et al DOI: 10.3969/j.issn.1006-6233.2025.12.09 Objective: To investigate the mechanism by which Ciwujia inhibits myocardial fibrosis in AS model rats through the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/peroxisome proliferator activated receptor gamma (PPAR gamma) signaling axis. Methods: A total of 44 SPF grade Wistar rats were selected for 6 weeks and randomly divided into a control group, a model group, a simvastatin group, and a Ciwujia group. In addition to the control group, the model group, simvastatin group and acanthopanax senticosus group were fed with high-fat diet combined with intraperitoneal injection of vitamin D3 to establish atherosclerosis (AS) model. After successful model replication, the control group and model group were given 5ml of physiological saline by gavage, the simvastatin group was given simvastatin solution by gavage, and the Ciwujia group was given a Ciwujia medicinal solution by gavage, with continuous intervention for 4 weeks. After the intervention, a small animal ultrasound machine was used to detect the rat heart for cardiac ultrasound examination. The length of the tibia (TL) was measured. The Heart Mass Index (HMI), Left Ventricular Mass Index (LVMI), and Lung Mass Index (LMI), as well as the ratio of heart mass to tibial length (HM/TL), left ventricular mass to tibial length (LV/TL), and lung mass to tibial length (LM/TL) were measured; HE staining was used to observe myocardial morphology, while Masson staining was used to observe the degree of myocardial fibrosis; TUNEL method was used to detect myocardial cell apoptosis; Immunofluorescence method was used to detect the positive expression of cAMP and PKA. Western blot technology was used to detect the expression of cAMP PKA signaling pathway related proteins. Results: Compared with the model group, the simvastatin group showed an increase in LVEF and LVFS (P<0.05), a significant decrease in HMI, LVMI, LMI, HM/TL, LV/TL, and LM/TL (P<0.05), a decrease in myocardial cell apoptosis rate (P<0.05), an increase in cAMP, PKA, and PPAR γ positive expression in myocardial tissue (P<0.05), and a decrease in MMP-9, TIMP-1, α - SMA, TGF- β1, ColI, and ColIII protein expression in myocardial tissue (P<0.05); Compared with the simvastatin group, the levels of LVEF and LVFS in the Ciwujia group were higher (P<0.05), while the levels of HMI, LVMI, LMI, HM/TL, LV/TL, and LM/TL were lower (P<0.05). The apoptosis rate of myocardial cells was lower (P<0.05), and the expression levels of cAMP, PKA, and PPAR γ in myocardial tissue were higher (P<0.05). The expression levels of MMP-9, TIMP-1, α - SMA, TGF-β1, ColI, and ColIII proteins in the myocardial tissue of rats in the Ciwujia group were lower (P<0.05). Conclusion: Ciwujia can inhibit the differentiation of cardiac fibroblasts and reduce the excessive accumulation of type I/III collagen fibers by activating the cAMP/PKA/PPAR γ signaling pathway, providing objective experimental evidence for the treatment of cardiovascular diseases. 2025 Vol. 31 (12): 1989-1997 [Abstract] ( 141 ) HTML (1 KB)  PDF (5065 KB)  ( 10 ) 1998 Study on Electroacupuncture Moxibustion Improving Post-Stroke Cognitive Dysfunction through mTOR / NLRP3 Mediated Autophagy YUAN Hongli, NIU Xianglai, XIAO Yaping, et al DOI: 10.3969/j.issn.1006-6233.2025.12.010 Objective: To explore the therapeutic effect and mechanism of electroacupuncture (EA) on cognitive dysfunction in stroke rats. Methods: Totally 42 SD rats were randomly divided into Sham group, MCAO group, MCAO+RAPA group, MCAO+EA group, MCAO+RAPA+EA group, MCAO+EA+Vector group, and MCAO+EA+OE-mTOR group, with 6 rats in each group. The middle cerebral artery embolization (MCAO) model was established, except for the Sham group. Rats in MCAO+RAPA group were intraperitoneally injected with 3 mg/kg RAPA, rats in MCAO+EA group were treated with electroacupuncture at Fengchi, Fengfu and Dashui points, and rats in MCAO+EA+Vector group and MCAO+EA+OE-mTOR group were injected with mTOR overexpression empty vector or mTOR overexpression vector in the lateral ventricle of the brain. The Zea-longa neurological deficit scoring system was used to evaluate neurobehavior. Morris water maze test was used to evaluate cognitive function. 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to evaluate the cerebral infarction area. Hematoxylin eosin (HE) staining was used to evaluate the pathological changes. Enzyme-linked immunosorbent assay was used to detect the contents of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10). Lens electron microscope was used to detect autophagosomes. Western blot was used to detect mTOR, IL-6, IL-10, NLRP3, Beclin-1, p62, LC3 Ⅰ/Ⅱ protein expression. Results: Compared with the Sham group, the Zea longa score, cerebral infarction area, TNF-α and IL-6 level, autophagosome number, Beclin-1, LC3 Ⅰ/Ⅱ, and NLRP3 protein levels in MCAO group increased, while the neurological function score, IL-10 content, p62 and mTOR protein levels decreased (P<0.05). Compared with MCAO group, the Zea-longa score, cerebral infarction area, TNF-α and IL-6 level, autophagosome number, Beclin-1, LC3 Ⅰ/Ⅱ and NLRP3 protein levels in MCAO+EA group decreased, while the neurological function score, IL-10 level, p62 and mTOR protein levels increased (P<0.05). Compared with MCAO group, the number of autophagosomes, the protein levels of beclin-1, LC3 Ⅰ/Ⅱ and NLRP3 increased, while the protein levels of p62 and mTOR decreased in MCAO+RAPA group (P<0.05). Compared with MCAO+EA+Vector group, the protein levels of Beclin-1, LC3 Ⅰ/Ⅱ and NLRP3 in MCAO+EA+OE-mTOR group increased, while the protein levels of p62 and mTOR decreased (P<0.05). Conclusion: EA can improve cognitive function and inflammatory state in MCAO rats, and the mechanism may be related to the upregulation of mTOR / NLRP3 pathway and inhibition of autophagy. 2025 Vol. 31 (12): 1998-2004 [Abstract] ( 155 ) HTML (1 KB)  PDF (2306 KB)  ( 7 ) 2004 Effect of LncRNA KCNQ1OT1 on Neurological Function of Rats with Cerebral Hemorrhage Model by Adjusting miR-214-3p/NDRG3 Axis GAO Xiaoheng, et al DOI: 10.3969/j.issn.1006-6233.2025.12.011 Objective: To investigate the effect of long non-coding RNA (LncRNA) KCNQ1OT1 on neurological function of intracerebral hemorrhage (ICH) model rats by adjusting microRNA-214-3p/N-myc downstream regulated gene 3 (miR-214-3p/NDRG3) axis. Methods: SD rats were assigned into sham surgery group (Sham), ICH group, LncRNA KCNQ1OT1 interference plasmid control (sh-NC) group, LncRNA KCNQ1OT1 interference plasmid (sh-KCNQ1OT1) group, sh-KCNQ1OT1+miR-214-3p inhibitor control (miR-In-NC) group, and sh-KCNQ1OT1+miR-214-3p inhibitor (miR-214-3p-In) group, each with 12 rats. The modified neurological severity score (mNSS) was used to assess changes in neurological function. The volume of cerebral hematoma in each group was measured. HE staining was performed to observe the pathological changes in brain tissue. TUNEL staining was used to observe cell apoptosis in brain tissue. The changes in the expression level of NDRG3 were observed by immunohistochemical staining. ELISA was used to detect the levels of TNF-α and IL-1β in the rat brain tissue. qRT-PCR was used to detect the levels of LncRNA KCNQ1OT1, miR-214-3p, and NDRG3 mRNA in the brain tissues. Western blot was performed to detect the expression of NDRG3, Bax, and Bcl-2 proteins in the brain tissues. Dual luciferase reporter gene assay was used to determine the targeting relationship between miR-214-3p and LncRNA KCNQ1OT1 or NDRG3. Results: For Sham group, the mNSS score, cerebral hematoma volume, and brain tissue apoptosis rate of rats in ICH group unusually increased (P<0.05), the brain tissue structure was disrupted, the numbers of red blood cells, inflammatory cell infiltration, and glial cells increased, the TNF-α, IL-1β, LncRNA KCNQ1OT1, NDRG3, and Bax in brain tissue were unusually increased (P<0.05), and the miR-214-3p and Bcl-2 were greatly reduced (P<0.05). For sh-NC group, the mNSS score cerebral hematoma volume, and brain tissue apoptosis rate of rats in sh-KCNQ1OT1 group greatly reduced (P<0.05), the brain tissue structure was restored, the numbers of red blood cells, inflammatory cell infiltration, and glial cells declined, the TNF-α, IL-1β, LncRNA KCNQ1OT1, NDRG3, and Bax in brain tissue were unusually declined (P<0.05), and the miR-214-3p and Bcl-2 were greatly raised (P<0.05). For sh-KCNQ1OT1+miR-In-NC group, the mNSS score, cerebral hematoma volume, and brain tissue apoptosis rate of rats in sh-KCNQ1OT1+miR-214-3p-In group greatly raised (P<0.05), the brain tissue structure was disrupted, the numbers of red blood cells, inflammatory cell infiltration, and glial cells raised, the TNF-α, IL-1β, NDRG3, and Bax in brain tissue were greatly raised (P<0.05), and the miR-214-3p and Bcl-2 were greatly reduced (P<0.05). Compared with the miR-NC group, the relative luciferase activity of cells transfected with KCNQ1OT1-WT or NDRG3-WT in the miR-214-3p group was significantly decreased. (P<0.05). Conclusion: Downregulation of LncRNA KCNQ1OT1 may alleviate neurological dysfunction in ICH rats by adjusting miR-214-3p/NDRG3 axis. 2025 Vol. 31 (12): 2004-2013 [Abstract] ( 142 ) HTML (1 KB)  PDF (2802 KB)  ( 13 ) 2014 Effect of Adjuvant Therapy with Osimertinib on the Efficacy and Levels of CCR7 TGF-β1 and CXCR5 in Patients with Advanced Non-Small Cell Lung Cancer ZHAO Min, ZHOU Xiaoyan, ZHANG Yanqing, et al DOI: 10.3969/j.issn.1006-6233.2025.12.012 Objective: To explore the clinical efficacy of osimertinib-adjuvant DP regimen in the treatment of patients with advanced non-small cell lung cancer (NSCLC), and to observe the changes of CCR7, TGF-β1 and CXCR5 levels, so as to provide scientific evidence for the clinical application of this drug. Methods: A retrospective analysis was conducted on clinical data from 98 patients with advanced NSCLC treated at our hospital from Januaryr 2021 to December 2022. The patients were divided into two groups based on treatment regimens: the control group (n=47) received afatinib in combination with the DP regimen (docetaxel+cisplatin), while the observation group (n=51) received osimertinib in combination with the DP regimen. The clinical efficacy and drug safety of the two groups were evaluated after 3 cycles of treatment. The survival rate within 2 years of follow-up was counted. The changes of immune function indexes [cluster of differentiation antigen 3 (CD3+), CD4+, CD8+, CD4+/CD8+] and CCR7, TGF-β1 and CXCR5 before and after treatment were observed, and the differences were analyzed. Results: The objective remission rate and disease control rate (52.94%, 88.24%) in observation group were higher than (32.65%, 72.34%) in control group (P<0.05). After 3 cycles of treatment, the levels of CD3+, CD4+, and CD4+/CD8+ in the two groups were increased, and the levels in observation group were higher than those in control group (P<0.05), and the levels of CD8+ was decreased, and the levels in observation group were lower (P<0.05). The levels of CCR7, TGF-β1 and CXCR5 after 1 cycle, 2 cycles and 3 cycles of treatment in the two groups were lower than those before treatment, and those in the observation group were lower than those in the control group at the above time points (P<0.05). The overall survival rates within 1 year and 2 years of follow-up in observation group (70.59%, 41.18%) were higher than those in control group (51.06%, 21.28%) (P<0.05). Conclusion: Compared with afatinib, Osimertinib-adjuvant DP regimen in the treatment of patients with advanced NSCLC can indeed enhance the short-term clinical efficacy and immune function, improve the long-term survival rate, and can effectively down-regulate the levels of CCR7, TGF-β1 and CXCR5, with safety. 2025 Vol. 31 (12): 2014-2019 [Abstract] ( 145 ) HTML (1 KB)  PDF (1176 KB)  ( 3 ) 2020 Mechanism of Metformin in Regulating the TLR4/NF-κB Signaling Pathway and Its Effect on Cartilage Degeneration in Post-Traumatic Osteoarthritis in Rats YUAN Zhifa, LIU Xunqi, ZHAO Xuesong, et al DOI: 10.3969/j.issn.1006-6233.2025.12.013 Objective: To explore the mechanism of action of metformin (MET) in regulating Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway and its effect on cartilage degeneration in post-traumatic osteoarthritis (PTOA) in rats. Methods: Totally 60 male Sprague-Dawley (SD) rats were randomly divided into five groups: control group, post-traumatic osteoarthritis (PTOA) group, MET group, TLR4 activator group (LPS group), and MET+LPS group, with 12 rats in each group. In the control group, only the skin lateral to the patella was incised and sutured. For the other groups, the PTOA rat model was established by transecting the cruciate ligaments and partially resecting the meniscus. After successful modeling, rats in the MET group and MET+LPS group were gavaged with 200mg/kg of metformin, while rats in the LPS group and MET+LPS group were gavaged with 50μg/kg of lipopolysaccharide (LPS). Rats in the control group and PTOA group were gavaged with an equal volume of saline, once daily for 4 consecutive weeks. After the last gavage, the morphology of articular cartilage in rats was observed using hematoxylin and eosin (HE) staining and safranin O-fast green staining, and the Osteoarthritis Research Society International (OARSI) score was assessed. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) in rat cartilage tissue. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect the expression levels of apoptosis-related genes (C-caspase-3, Bax, Bcl-2) and mRNA expression levels of SOX9, MMP-13, and ADAMTS5 in rat cartilage tissue. Western blot analysis was conducted to detect the protein expression levels of TLR4, MyD88, NF-κB p65, and p-NF-κB p65 in rat cartilage tissue. Results: Compared with the control group, the PTOA group exhibited severe cartilage damage and degeneration, with significantly increased OARSI scores, levels of pro-inflammatory cytokines in cartilage tissue, and expression of C-caspase-3, Bax, MMP-13, ADAMTS5 mRNA, as well as TLR4, MyD88, and p-NF-κB p65/NF-κB p65 (P<0.05). Additionally, the expression of Bcl-2 and SOX9 mRNA was significantly decreased in the PTOA group (P<0.05). In comparison to the PTOA group, the MET group showed significantly reduced cartilage damage and degeneration, with decreased OARSI scores, levels of pro-inflammatory cytokines in cartilage tissue, and expression of C-caspase-3, Bax, MMP-13, ADAMTS5 mRNA, as well as TLR4, MyD88, and p-NF-κB p65/NF-κB p65 (P<0.05). Furthermore, the expression of Bcl-2 and SOX9 mRNA was significantly increased in the MET group (P<0.05). Conversely, the LPS group exhibited opposite results for these indicators (P<0.05). MET reversed the promoting effects of LPS on cartilage degeneration, inflammation, and chondrocyte apoptosis in PTOA rats. Conclusion: MET can ameliorate cartilage degeneration in PTOA rats by inhibiting the TLR4/NF-κB signaling pathway, reducing joint inflammation, inhibiting chondrocyte apoptosis, and decreasing the degradation of chondrocyte extracellular matrix. 2025 Vol. 31 (12): 2020-2026 [Abstract] ( 131 ) HTML (1 KB)  PDF (1464 KB)  ( 5 ) 2027 Effect of Fucoxanthin on Insulin Resistance in Gestational Diabetes Mellitus Rats by Regulating Notch/Snail1 Signaling Pathway GAO Dandan, JIANG Haiying, LI Juan DOI: 10.3969/j.issn.1006-6233.2025.12.014 Objective: To explore the effect of fucoxanthin (FUC) on insulin resistance in gestational diabetes mellitus rats by regulating Notch/zinc finger transcription factor 1 (Snail1) signaling pathway. Methods: A GDM rat model was constructed, and successfully modeled rats were grouped into GDM group, L-FUC, M-FUC, H-FUC groups (gavage of 13, 26, and 65 mg/kg FUC), and H-FUC+VPA group (gavage of 65 mg/kg FUC+intraperitoneal injection of 300 mg/kg Notch activator VPA), with 10 rats in each group. Another 10 normal pregnant mice served as the control group. The control group and the GDM group were administered the same volume of a mixture prepared with 10% DMSO, 40% PEG300, 5% Tween 80, and 45% saline once a day for 2 consecutive weeks. A blood glucose meter was used to detect the FBG and FINS in rats, and the IRI was calculated. The reagent kits were used to detect TG and TC in rats. HE staining was used to observe the morphological changes in rat liver tissue. ELISA was used to detect serum TNF-α and IL-6 in rats. Western blot was used to detect changes in Notch/Snail1 signaling pathway proteins in rat liver tissue.Results: For the control group, the GDM group showed vacuolar necrosis of liver tissue cells, accompanied by a large amount of inflammatory cell infiltration. For the GDM group, the L-FUC group, M-FUC group, and H-FUC group showed a decrease in vacuolar phenomenon and infiltration of inflammatory cells in liver tissue cells. The H-FUC+VPA group showed more severe liver tissue pathological damage and increased inflammatory cell infiltration than the H-FUC group. The GDM group showed higher FBG, FINS, IRI, TC, TG, TNF-α, IL-6, and liver tissue Notch and Snail1 proteins than the control group (P<0.05). The L-FUC group, M-FUC group, and H-FUC group showed decreased FBG, FINS, IRI, TC, TG, TNF-α, IL-6, and liver tissue Notch and Snail1 proteins than the GDM group, in a dose-dependent manner (P<0.05). The H-FUC+VPA group showed higher FBG, FINS, IRI, TC, TG, TNF-α, IL-6, and liver tissue Notch and Snail1 proteins than the H-FUC group (P<0.05). Conclusion: FUC can improve insulin resistance in GDM rats, which may be mainly based on the inhibitory effect of the Notch/Snail1 signaling pathway. 2025 Vol. 31 (12): 2027-2032 [Abstract] ( 133 ) HTML (1 KB)  PDF (1440 KB)  ( 7 ) 2032 Effects of Icariin on in Vitro Osteogenic Differentiation and PI3K/AKT Pathway of Rat Bone Marrow Mesenchymal Stem Cells JI Wei, LIU Yuanhang, CAI Chenlin, et al DOI: 10.3969/j.issn.1006-6233.2025.12.015 Objective: To investigate the effects of icariin (ICA) on in vitro osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) and the phosphatidylinositol 3-kinase (PI3K)/serine threonine protein kinase (AKT) pathway. Methods: The third generation of rat BMSCs were randomly assigned into the control group (cultured in DMEM medium), dexamethasone (DXMS) group (cultured in DMEM medium supplemented with 10-6mol/L DXMS), L-ICA, M-ICA, H-ICA groups (DXMS induced DMEM medium+10-8, 10-7, 10-6mol/L ICA), and ICA+740Y-P group (DXMS induced DMEM medium+10-6mol/L ICA+30μmol/L PI3K activator 740Y-P). CCK8 method was used to examine the proliferation of rat BMSCs. Alizarin red staining was used to examine the mineralization ability of rat BMSCs induced by bone orientation (cultured in DMEM medium with a final concentration of 100nM DXMS+50μM vitamin+2 mM L-glutamine+10mM β-glycerophosphate). ELISA kit was used to examine the expression of ALP, ROS, and SOD in rat BMSCs. Western blot was used to examine the expression of PI3K/AKT pathway proteins in rat BMSCs. Results: The cytoplasmic staining in the DXMS group was relatively light, with lower A450 values, ALP, and SOD compared to the control group, while ROS, p-PI3K/PI3K, and p-AKT/AKT were higher than those in the control group (P<0.05); in the L-ICA group, M-ICA group, and H-ICA group, the cytoplasmic staining deepened, with higher A450 values, ALP, and SOD compared to the DXMS group, while ROS, p-PI3K/PI3K, and p-AKT/AKT were lower than those in the DXMS group (P<0.05); in the ICA + 740Y-P group, the cytoplasmic staining became lighter, with lower A450 values, ALP, and SOD compared to the H-ICA group, while ROS, p-PI3K/PI3K, and p-AKT/AKT were higher than those in the H-ICA group (P<0.05). Conclusion: ICA may inhibit the PI3K/AKT pathway and promote osteogenic differentiation of rat BMSCs. 2025 Vol. 31 (12): 2032-2037 [Abstract] ( 161 ) HTML (1 KB)  PDF (1734 KB)  ( 5 ) 2037 Correlation of Blood Tumor Marker Levels with Clinical Efficacy of TACE Interventional Therapy for Primary Liver Cancer and Its Postoperative Residual Cancer Prediction Value CHEN Tian, ZHANG Yu, LI Tingting, et al DOI: 10.3969/j.issn.1006-6233.2025.12.016 Objective: To investigate the correlation between the levels of blood tumor markers and the effect of TACE intervention for primary liver cancer (PLC) and the predictive value of the presence of residual cancer after surgery. Methods: Totally 132 primary liver cancer patients were selected as the study subjects, and they were divided into the valid group (n=40) and the ineffective group (n=92) according to the presence or absence of residual cancer after the operation, and the clinical data of the patients were collected, and the levels of serum tumor markers were detected before and after the treatment. Before and after therapy, the two groups' clinical features and blood tumor marker levels were compared; the influencing factors of the efficacy of PLC after surgery were analyzed by multifactorial logistic regression; and the predictive value of blood tumor markers for residual cancers after surgery was assessed by using the Receiver Operating Characteristic (ROC) curves. Results: The patients in the two groups had BMI, gender and the presence of cirrhotic nodules were not significant (P>0.05); the ratio of patients with differentiation degree Ⅲ~Ⅳ, as well as the levels of blood tumor markers AFP, CEA, CA125 and CA50 in the valid group were significantly higher than those in the valid group, and the tumor envelope proportion (P<0.05) was much lower than that in the successful group. Following TACE administration, there was a statistically significant difference (P<0.05) between the ineffective group's and the valid group's levels of the blood tumor markers AFP, CEA, CA125, and CA50; the results of multifactorial logistic regression analysis showed that the high levels of AFP, CA50 and CEA as well as the high degree of differentiation of the tumors were the risk factors affecting the efficacy of the treatment (P<0.05), and the tumor envelope was the protective factor for the efficacy of the treatment (P<0.05). The ROC curve showed that the area under the curve (AUC) of AFP, CEA, CA125 and CA50 for the diagnosis of residual cancer after PLC intervention were 0.917, 0.925, 0.711, and 0.617, respectively. Conclusion: The level of blood tumor markers is related to the curative effect of TACE and postoperative residual cancer, and the AUC of AFP and CEA is closer to 1, which shows that AFP and CEA have high diagnostic value for the occurrence of residual cancer after PLC intervention, and provide an important basis for the curative effect evaluation and postoperative residual cancer prediction of primary liver cancer treated by TACE 2025 Vol. 31 (12): 2037-2043 [Abstract] ( 136 ) HTML (1 KB)  PDF (1406 KB)  ( 7 ) 2043 Expression of miR-375 and SLC7A11 in Breast Cancer Tissue and Their Relationship with Pathological Subtypes and Prognosis GUO Dongxu, SHANG Yue, QI Zhonghua DOI: 10.3969/j.issn.1006-6233.2025.12.017 Objective: To investigate the relationship between the expression of miR-375 and solute carrier family 7 member 11 (SLC7A11) in breast cancer tissues with pathological subtypes and prognosis. Methods: A total of 150 breast cancer patients who underwent radical mastectomy in Fuxin Mining General Hospital of Liaoning Health Industry Group from January 2017 to March 2021 were selected. Real-time quantitative polymerase chain reaction was used to detect the expression of miR-375 and SLC7A11 messenger RNA (mRNA) in breast cancer tissue and corresponding adjacent normal tissues. Pearson correlation analysis was performed to evaluate the correlation between miR-375 and SLC7A11 mRNA expression in breast cancer tissue. Patients were divided into high- and low-expression groups based on the median expression levels of miR-375 and SLC7A11 mRNA. Kaplan-Meier curves were used to analyze progression-free survival (PFS) in different groups. Multivariate Cox regression analysis was conducted to identify prognostic factors for breast cancer patients. Results: Compared to adjacent normal tissues, miR-375 expression was significantly down-regulated, whereas SLC7A11 mRNA expression was significantly up-regulated in breast cancer tissue (P＜0.05). A negative correlation was observed between miR-375 and SLC7A11 mRNA expression in breast cancer tissue (r＝－0.757,P＜0.05). miR-375 expression was lower, and SLC7A11 mRNA expression was higher in poorly differentiated tumors, TNM stage III tumors, and tumors with lymph node metastasis compared to moderately-to-well differentiated tumors, TNM stage I–II tumors, and tumors without lymph node metastasis (P＜0.05). The 3-year PFS rate among the 150 breast cancer patients was 75.33% (113/150). Patients with high miR-375 expression had a significantly higher 3-year PFS rate (90.79%) compared to those with low miR-375 expression (59.46%), while patients with high SLC7A11 mRNA expression had a significantly lower 3-year PFS rate (60.98%) compared to those with low SLC7A11 mRNA expression (92.65%) (P＜0.05). Multivariate Cox regression analysis showed miR-375 ≥1.10 was identified as an independent protective factor, whereas SLC7A11 mRNA ≥1.05 was an independent risk factor for poor prognosis in breast cancer patients (P＜0.05). Conclusion: Low expression of miR-375 and high expression of SLC7A11 mRNA in breast cancer tissues are associated with unfavorable pathological subtypes and poor prognosis. 2025 Vol. 31 (12): 2043-2049 [Abstract] ( 145 ) HTML (1 KB)  PDF (1351 KB)  ( 7 ) 2049 Influencing Factors of Adverse Outcomes in Patients with Chronic Obstructive Pulmonary Disease and Predictive Value of Serum NLR PA and PCT Levels HE Jie, LIU Jing, ZHAO Rui, et al DOI: 10.3969/j.issn.1006-6233.2025.12.018 Objective: To analyze the influencing factors of adverse outcomes in patients with chronic obstructive pulmonary disease (COPD), and to explore the predictive value of serum lymphocyte-to-lymphocyte ratio (NLR), prealbumin (PA), and procalcitonin (PCT) levels on adverse outcomes. Methods: Totally 102 patients with COPD admitted to the hospital from April 2020 to April 2023 were divided into a good prognosis group (n=87) and a poor prognosis group (n=15) according to the clinical outcomes. Univariate and multivariate logistic regression analyses were used to analyze the factors influencing adverse outcomes of patients, and the receiver characteristic operating curve (ROC) was used to analyze the predictive efficacy of PA and PCT levels for adverse outcomes. Results: The age, number of acute exacerbation hospitalizations in the past 1 year, concurrent respiratory failure, disturbance of consciousness, NLR, mMRC grading, PA, PCT, LVEF, FEV1 and FEV1/FVC exhibited statistical differences between groups (P<0.05). Logistic regression analysis indicated that age, number of acute exacerbation hospitalizations in the past 1 year, concurrent respiratory failure, disturbance of consciousness, mMRC grading, NLR, PCT and low LVEF, FEV1 and FEV1/FVC were high risk factors for adverse outcomes in patients with COPD, and the elevation of PA is a protective factor (OR=1.839, CI: 0.992-3.409; OR=2.090, CI: 1.107-3.943; OR=2.484, CI: 1.224-5.041; OR=2.125, CI: 1.100-4.106; OR=4.195, CI: 2.048-8.597; OR=2.493, CI: 1.355-4.587; OR=2.227, CI: 1.418-3.498; OR=0.958, CI: 0.919-0.998; OR=0.950, CI: 0.914-0.988; OR=0.946, CI: 0.905-0.990; OR=0.943, CI: 0.901-0.986, P<0.05). ROC curve analysis revealed that the area under the curve (AUC), sensitivity, and specificity of the combination of PCT, PA, and NLR in predicting adverse outcomes were 0.939, 93.33% and 93.10%, which were significantly better than those of each indicator alone (P<0.05). Conclusion: The adverse outcomes of patients with COPD are affected by age, mMRC grading, PA, PCT, NLR, and pulmonary function indicators. The combined detection of PA, PCT, and NLR can enhance the predictive value of adverse outcomes. 2025 Vol. 31 (12): 2049-2055 [Abstract] ( 139 ) HTML (1 KB)  PDF (1336 KB)  ( 13 ) 2055 Correlation between Serum SOX2 SIRT1 and Cellular Immunity Level in Non-Small Cell Lung Cancer Patients MU Qi'er, HAN Yanru DOI: 10.3969/j.issn.1006-6233.2025.12.019 Objective: To investigate the correlation and regulatory mechanism between SOX2 and SIRT1 and the cellular immunity level of non-small cell lung cancer patients. Methods: 100 patients with stage III-IV non-small cell lung cancer were included in the study. The spatial heterogeneity of SOX2 expression in tumor tissue was analyzed by immunohistochemistry, and the regulatory effects of SOX2 on immunosuppressive factors, metabolites, and T cell depletion were evaluated using mass spectrometry, flow cytometry, and Luminex multiplex detection omics techniques. Results: The proportion of SOX2 positive cells in the central region of lung squamous cell carcinoma was 82.3%±6.7%, with an H-score of 246±18, significantly higher than that in the peripheral region (41.2%±9.1, P=0.003). The concentration of interleukin-10 in the SOX2 high expression group (34.6±8.5 pg/mL) increased by 2.81 times compared to the control group, and the increase in serum SOX2 concentration led to an increase in the proportion of regulatory T cells from 5.2%±4.1% to 9.4%±0.4% (P<0.05). Conclusion: SOX2 promotes the formation of an immunosuppressive microenvironment by driving metabolic immune imbalance, while SIRT1 suppresses T cell function through epigenetic regulation. The two synergistically mediate immune escape in non-small cell lung cancer, providing a new strategy for targeted intervention. 2025 Vol. 31 (12): 2055-2062 [Abstract] ( 131 ) HTML (1 KB)  PDF (2308 KB)  ( 9 ) 2062 Relationship between miR-1323/IL-6 Axis Factor and Prognosis in Elderly Patients with Severe Bacterial Pneumonia and the Predictive Significance SUN Xun, WANG Tianyi, et al DOI: 10.3969/j.issn.1006-6233.2025.12.020 Objective: To investigate the relationship between the expression levels of peripheral blood microRNA-1323 (miR-1323)/interleukin-6 (IL-6) inflammatory axis factors and prognosis, as well as their predictive significance in elderly patients with severe pneumonia (SP). Methods: A total of 158 elderly patients with SP admitted from August 2020 to August 2024 were selected and followed up for 30 days. According to the prognosis, they were divided into good prognosis group and poor prognosis group. The clinical data immediately after admission, the levels of miR-1323, IL-6 and soluble interleukin-6 receptor (sIL-6R) in peripheral blood on the next day of admission were compared between the two groups. Smooth curve fitting was used to analyze the correlation between the miR-1323/IL-6 inflammatory axis factors in peripheral blood and prognosis. Logistic regression analysis was conducted to identify the influencing factors of prognosis in elderly SP patients. Receiver Operating Characteristic (ROC) curves and Area Under the Curve (AUC) were used to evaluate the predictive value of the miR-1323/IL-6 inflammatory axis factors in peripheral blood for prognosis. Results: After a 30-day follow-up, 112 cases had a good prognosis, while 46 cases had a poor prognosis. The poor prognosis group had higher age, CPIS scores, and CRP levels, and a longer duration of mechanical ventilation than the good prognosis group (P<0.05). The levels of miR-1323, IL-6, and sIL-6R in peripheral blood were higher in the poor prognosis group than in the good prognosis group (P<0.05). Smooth curve fitting analysis showed a positive linear relationship between the levels of miR-1323, IL-6, and sIL-6R in peripheral blood and the risk of poor prognosis in elderly SP patients, with a gradual increase in the risk of poor prognosis as the levels of miR-1323, IL-6, and sIL-6R in peripheral blood increased (P<0.05). Logistic regression analysis showed that, both before and after adjusting for other factors, miR-1323, IL-6, and sIL-6R in peripheral blood were independent factors affecting the prognosis of elderly SP patients (P<0.05). ROC curve analysis revealed that the AUCs for predicting the prognosis of elderly SP patients using miR-1323, IL-6, and sIL-6R in peripheral blood were 0.727 (95%CI: 0.650-0.794), 0.749 (95%CI: 0.674-0.815), and 0.780 (95%CI: 0.707-0.842), with sensitivities of 63.04%, 63.04%, and 60.87%, respectively, and specificities of 75.89%, 79.46%, and 86.61%. The AUC for predicting the prognosis of elderly SP patients using a combination of the three factors was 0.881 (95%CI: 0.820-0.927), with a sensitivity of 71.74% and a specificity of 85.71%, significantly higher than that of individual factors (Z=2.888, 2.693, 2.075, P=0.004, 0.007, 0.038). Conclusion: The miR-1323/IL-6 inflammatory axis factor in peripheral blood has a positive linear relationship with the risk of poor prognosis in elderly patients with bacterial SP. It can be used as an independent predictor to help effectively predict the risk of poor prognosis in clinical practice, and the combined predictive value is more reliable. 2025 Vol. 31 (12): 2062-2067 [Abstract] ( 140 ) HTML (1 KB)  PDF (1387 KB)  ( 6 ) 2068 The Value of Serum NT-proBNP Erythrocyte Sedimentation Rate Albumin Combined with LDH in Evaluating Kawasaki Disease Complicated with Coronary Artery Lesions FANG Wenjing, WANG Dandan, et al DOI: 10.3969/j.issn.1006-6233.2025.12.021 Objective: To explore the value of serum N-terminal pro-B-type natriuretic peptide (NT-proBNP), erythrocyte sedimentation rate (ESR),albumin(ALB), and lactate dehydrogenase (LDH) in evaluating Kawasaki disease (KD) with coronary artery lesions (CAL). Methods: The clinical data of 149 patients who were diagnosed with KD and received cardiac ultrasound examination in the hospital from October 2023 to March 2025 were retrospectively analyzed. According to the results of ultrasound examination, the enrolled patients were divided into CAL group (KD with CAL, n=38) and non-CAL group (KD without CAL, n=111). The clinical data were compared between the CAL group and the non-CAL group. The differences in serum NT-proBNP, ESR, creatine kinase (CK), creatine kinase-MB (CK-MB), LDH, aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood sodium (Na), and ALB were compared between the two groups by t-test or rank sum test. Multivariate Logistic regression analysis was performed to analyze the relationship between the above laboratory indicators and KD with CAL. The area under the receiver operating characteristic (ROC) curve (AUC) was used to evaluate the value of NT-proBNP, ESR, LDH, and ALB in predicting KD with CAL. Results: NT-proBNP, ESR, AST, ALT, and LDH in the CAL group were higher than those in the non-CAL group, while ALB and Na were lower than those in the non-CAL group, with statistical differences (P<0.05). Multivariate Logistic regression analysis indicated that NT-proBNP (OR=1.003, 95%CI: 1.001~1.005), ESR (OR=1.181, 95%CI: 1.079~1.292), LDH (OR=1.080, 95%CI: 1.036~1.125), and ALB (OR=0.584, 95%CI: 0.400~0.855) were the influencing factors of KD with CAL (P<0.05). The AUCs of NT-proBNP, ESR, LDH and ALB for predicting KD with CAL were 0.856 (95%CI: 0.789~0.908), 0.733 (95%CI: 0.654~0.802), 0.720 (95%CI: 0.641~0.791) and 0.800 (95%CI: 0.727~0.861), respectively (P<0.05), and the AUC of the combination of the above indicators for predicting KD with CAL was 0.980 (95%CI: 0.943~0.996) (P<0.05). Conclusion: NT-proBNP, ESR, LDH, and ALB are influencing factors of CAL occurrence in children with KD, and can be used as predictors of CAL. 2025 Vol. 31 (12): 2068-2073 [Abstract] ( 144 ) HTML (1 KB)  PDF (1399 KB)  ( 4 ) 2073 Correlation of Shoulder Synovial Fluid TNF-α and LTB4 Expression with Pain and Function in Patients with Type 2 Diabetes Mellitus Combined with Rotator Cuff Injury CHEN Tao, et al DOI: 10.3969/j.issn.1006-6233.2025.12.022 Objective: To investigate the expression of tumor necrosis factor-α (TNF-α) and leukotriene B4 (LTB4) in the synovial fluid of the shoulder joints of patients with type 2 diabetes mellitus combined with rotator cuff injuries preoperatively and their correlation with shoulder pain and function. Methods: Sixty-eight patients with rotator cuff injuries treated with concurrent arthroscopic surgery admitted to Chengde City Central Hospital from January 2024 to March 2025 were prospectively included and divided into diabetic (n=32) and non-diabetic (n=36) groups according to whether they had type 2 diabetes mellitus. Enzyme-linked immunosorbent assay (ELISA) was used to detect the differences in the levels of TNF-α and LTB4 in the synovial fluid of the shoulder joints of the patients in the two groups, and to evaluate and compare the differences in the preoperative VAS scores of shoulder pain, the American Society of Shoulder and Elbow Surgeons (ASES) scores, the Constant Shoulder Score of the two groups, and the correlations between each score and TNF-α and LTB4 in the synovial fluid of the shoulder joints were analyzed by Pearson correlation-the correlation between each score and shoulder synovial fluid TNF-α, LTB4. Results: There was no statistical significant difference in the general information such as gender, age, body mass index, injury side, and tear size between the two groups (P>0.05). In the diabetic group, shoulder synovial fluid TNF-α, LTB4, and VAS scores were higher than those in the nondiabetic group, and ASES and Constant scores were lower than those in the nondiabetic group, and the differences were statistically significant (P<0.05). There is a positive correlation between the preoperative VAS scores and the levels of synovial fluid TNF-α and LTB4 in the shoulder joint among the 68 patients with rotator cuff injuries (P<0.05); preoperative ASES score and Constant score were negatively correlated with shoulder synovial fluid TNF-α and LTB4 levels (P<0.05). Conclusion: In patients with rotator cuff injury combined with type 2 diabetes mellitus, the preoperative levels of TNF-α and LTB4 in the synovial fluid of the shoulder joint were significantly elevated and positively correlated with joint pain and negatively correlated with functional scores, suggesting that diabetes mellitus may exacerbate localized inflammatory responses in the shoulder joint of rotator cuff injury patients and aggravate the pain and dysfunction. 2025 Vol. 31 (12): 2073-2078 [Abstract] ( 147 ) HTML (1 KB)  PDF (1303 KB)  ( 5 ) 2078 Analysis of Preoperative Clinical Characteristics and Postoperative Predictive Value of Patients with Primary Angle-Closure Glaucoma Complicated with Cataract DONG Yunyun, DENG Ji'an, YANG Yang DOI: 10.3969/j.issn.1006-6233.2025.12.023 Objective: To investigate the preoperative clinical features and postoperative predictive value of primary angle-closure glaucoma (PACG) patients with cataract. Methods: A total of 107 patients with PACG complicated with cataract who underwent surgical treatment in our hospital from October 2021 to November 2024 were selected. According to the prognosis at 3 months after surgery, they were divided into good prognosis group and poor prognosis group. Univariate analysis was used to analyze the preoperative clinical characteristics of the two groups. Multivariate logistic regression analysis was used to analyze the correlation between various influencing factors and poor postoperative prognosis in patients with PACG and cataract, and ROC curve was used to evaluate its predictive value. Results: The poor prognosis group had a higher percentage of complete closure of the anterior chamber angle, hypertension history, diabetes history, hyperlipidemia history, smoking history, and drinking history compared to the good prognosis group. Additionally, the intraocular pressure fluctuated significantly (P<0.05), the anterior chamber depth was shallower, and the axial length was shorter (P<0.05). Preoperative hyperlipidemia history, shallow anterior chamber depth, short axial length and large fluctuation of intraocular pressure were the influencing factors of poor prognosis in PACG patients with cataract (P<0.05). ROC curve analysis showed that the detection efficiency of hyperlipidemia history, anterior chamber depth, axial length and intraocular pressure fluctuation was higher, and the combined detection was higher than that of single detection. Conclusion: Preoperative hyperlipidemia history, anterior chamber depth, axial length and intraocular pressure fluctuation are closely related to the poor prognosis of PACG patients with cataract, and the prediction efficiency is high. 2025 Vol. 31 (12): 2078-2084 [Abstract] ( 150 ) HTML (1 KB)  PDF (1341 KB)  ( 10 ) 2084 Correlation between Umbilical Artery Blood Gas Analysis and Neonatal Hypoxic-Ischemic Myocardial Injury SHI Yanhong, XIN Haiyan, LIN Hong DOI: 10.3969/j.issn.1006-6233.2025.12.024 Objective: To investigate the correlation between umbilical artery blood gas analysis and neonatal hypoxic-ischemic myocardial injury. Methods: A total of 110 neonates admitted to our hospital from January 2023 to June 2025 were selected as the research objects. According to whether they had hypoxic-ischemic myocardial injury, they were divided into hypoxic-ischemic myocardial injury group (n=48) and non-hypoxic-ischemic myocardial injury group (n=62). The clinical data and umbilical artery blood gas analysis levels were compared between the two groups. The relationship between the umbilical artery blood gas analysis index and myocardial enzyme spectrum index was analyzed by Pearson correlation analysis. Results: The levels of hydrogen ion concentration index (pH), base excess (BE), partial pressure of oxygen (PaO2), bicarbonate (HCO3-), calcium ion, and chloride ion in the hypoxic-ischemic myocardial injury group were lower than those in the non-hypoxic-ischemic myocardial injury group (P<0.05). The levels of partial pressure of carbon dioxide (PaCO2), creatine phosphokinase (CK), creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), and alanine aminotransferase (ALT) were increased (P<0.05). Pearson correlation analysis showed that umbilical artery blood gas analysis was closely associated with the myocardial enzyme spectrum index (P<0.05). Conclusion: The level of umbilical artery blood gas analysis index is closely associated with neonatal hypoxic-ischemic myocardial injury. 2025 Vol. 31 (12): 2084-2089 [Abstract] ( 141 ) HTML (1 KB)  PDF (1185 KB)  ( 5 ) 2089 Analysis of the Relationship and Predictive Value between the TFD Value and Postoperative Fixation Failure in Patients Undergoing TGN Internal Fixation for Intertrochanteric Femoral Fracture LI Wujian, HE Shaobo, REN Cong, et al DOI: 10.3969/j.issn.1006-6233.2025.12.025 Objective: To investigate the relationship between distance between tail femur distance (TFD) value and postoperative fixation failure in patients with femoral intertrochanteric fractures treated with the third-generation Gamma nail (TGN) internal fixation. Methods: The case data of 160 patients with femoral intertrochanteric fractures treated with TGN internal fixation in the hospital from January 2022 to August 2024 were retrospectively analyzed. According to the postoperative fracture healing status, the patients were divided into normal healing group (n=128) and fixation failure group (n=32). The differences in general data and TFD value were recorded and compared between groups. Multivariate logistic regression analysis was used to analyze the independent influencing factors of postoperative fixation failure. Receiver operating characteristic curve (ROC) was drawn to analyze the value of the above statistically significant indicators on predicting postoperative fixation failure. Results: At 6 months after surgery, the proportions of patients with normal fracture healing, patients with postoperative lag screw migration and patients with postoperative lag screw cutting-out were 80.00% (128/160), 12.50% (20/160) and 7.50% (12/160), respectively. Comparison of general data found that the bone mineral density T value in normal healing group was higher than that in fixation failure group, and the TFD value was shorter than that in fixation failure group (P<0.05). Multivariate Logistic regression analysis showed that bone mineral density T value and TFD value were independent influencing factors of postoperative fixation failure (P<0.05). ROC curve analysis showed that the areas under the curves (AUCs) of bone mineral density T value and TFD value in predicting postoperative fixation failure were 0.818 and 0.906, respectively. Conclusion: The fixation failure after TGN internal fixation of femoral intertrochanteric fractures is closely related to bone mineral density T value and TFD value, and has certain predictive value on postoperative fixation failure. 2025 Vol. 31 (12): 2089-2093 [Abstract] ( 140 ) HTML (1 KB)  PDF (1379 KB)  ( 4 ) 2093 Effect of Intravenous Infusion Lidocaine and Laryngeal Mask Combined with Bronchial Blocker in Thoracoscopic Partial Lobectomy on Quality of Recovery and Postoperative Sore Throat WANG Liping, SUN Yanbin, GUAN Sen, et al DOI: 10.3969/j.issn.1006-6233.2025.12.026 Objective: To investigate the effect of intravenous lidocaine combined with laryngeal mask airway (LMA) and bronchial blocker on postoperative sore throat and recovery quality in patients undergoing video-assisted thoracoscopic (VATS) partial lung resection. Methods: A total of 75 patients scheduled for elective VATS partial lung resection in our hospital from July 2023 to June 2024 were selected, with American Society of Anesthesiologists (ASA) physical status classification Ⅰ~Ⅲ, body mass index (BMI) 18.0-30.0 kg/m2, and age 18-65 years. According to the random number table method, the patients were divided into three groups with 25 cases in each group: endotracheal intubation combined with bronchial blocker group (Group E), LMA combined with bronchial blocker group (Group B), and lidocaine combined with LMA and bronchial blocker group (Group L). Group E achieved lung isolation using endotracheal intubation combined with a bronchial blocker, while Groups B and L performed lung isolation with LMA combined with a bronchial blocker. For Group L, a loading dose of 1.5mg/kg lidocaine was intravenously pumped 15 minutes before anesthesia induction, followed by continuous intravenous pumping of lidocaine at 1.5mg/kg/h during anesthesia maintenance until the end of the operation; Groups E and B were pumped with the same dose of normal saline instead of lidocaine, and the remaining treatments were identical among the three groups. The following parameters were observed and recorded: Quality of Recovery-15 (QoR-15) scores before surgery, at 24 hours and 48 hours after surgery; Visual Analogue Scale (VAS) scores for postoperative sore throat; mean arterial pressure (MAP) and heart rate (HR) at the time points of before surgery (T0), immediately after insertion of the lung isolation device (T1), skin incision (T2), start of one-lung ventilation (T3), end of one-lung ventilation (T4), and extubation (T5); serum levels of interleukin-6 (IL-6) and C-reactive protein (CRP) before surgery, at 24 hours and 48 hours after surgery; intraoperative dosages of propofol and remifentanil; number of presses of patient-controlled intravenous analgesia (PCIA) pump within 48 hours after surgery; and incidence of postoperative adverse reactions. Results: Compared with Group E, the QoR-15 scores at 24 hours after surgery in Groups B and L were significantly higher, and the QoR-15 score of Group L at 48 hours after surgery was higher than that of Group B; the VAS scores for postoperative sore throat in Groups L and B were significantly lower, and the score of Group L was lower than that of Group B; the fluctuations of MAP and HR at T1 and T5 in Groups L and B were smaller; at 24 hours and 48 hours after surgery, the serum levels of IL-6 and CRP in Groups L and B were decreased; the intraoperative dosage of remifentanil in Group L was reduced; and the number of PCIA pump presses in Group L after surgery was decreased, which was fewer than that in Group B. Conclusion: Intravenous lidocaine combined with LMA and bronchial blocker in VATS partial lung resection is beneficial for postoperative recovery of patients and can alleviate postoperative sore throat. 2025 Vol. 31 (12): 2093-2099 [Abstract] ( 135 ) HTML (1 KB)  PDF (1216 KB)  ( 3 ) 2099 Analysis of the Predictive Value of Syndecan-1 Combined with vWF for Acute Traumatic Coagulopathy LIU Ying, WU Jun DOI: 10.3969/j.issn.1006-6233.2025.12.027 Objective: To evaluate the predictive value of syndecan-1 (Syndecan-1) combined with vascular hemophilia factor (vWF) in predicting acute trauma coagulopathy (ATC). Methods: A total of 238 acute trauma patients admitted to our hospital's emergency department between March and December 2024 were selected and divided into ATC group (76 cases) and non-ATC group (162 cases) based on ATC occurrence. Clinical data differences were compared univariately, multivariate logistic regression was performed to analyze factors influencing ATC development, and ROC curves were plotted to assess the predictive value of Syndecan-1 combined with vWF. Results: Among the 238 patients, 76 (31.9%) developed ATC. Univariate analysis showed statistically significant differences in prothrombin time (PT), activated partial thromboplastin time (APTT), Syndecan-1, and vWF levels between ATC and non-ATC groups (P<0.05). Multivariate analysis confirmed these biomarkers as significant predictors of ATC development (P<0.05). ROC curve analysis indicated a 0.781(95%CI:0.808-0.934 )area under the curve for Syndecan-1 combined with vWF, with sensitivity of 0.856 and specificity of 0.823. Conclusion: Syndecan-1 combined with vWF demonstrates predictive value for ATC development in acute trauma patients. 2025 Vol. 31 (12): 2099-2103 [Abstract] ( 150 ) HTML (1 KB)  PDF (1296 KB)  ( 5 ) 2103 Comparative Study of Ultrasonographic Manifestations and Surgical Pathological Results of Hepatic Cystic Echinococcosis ZHENG Jing, ZHAN Yan, MA Zhi, et al DOI: 10.3969/j.issn.1006-6233.2025.12.028 Objective: To observe the ultrasonographic manifestations of hepatic cystic echinococcosis, and to compare with the results of surgical pathology. Methods: The clinical data of 158 patients (158 lesions) with hepatic cystic echinococcosis in the Ganzi Tibetan Autonomous Prefecture People's Hospital from January 2022 to December 2024 were retrospectively analyzed. All patients received ultrasound examination before surgery, and their ultrasonographic data were collected and the involvement conditions of lesion in blood vessels and bile ducts were assessed. The diagnostic efficiency of ultrasound on hepatic cystic echinococcosis typing and detection conditions of vascular involvements and bile duct involvements were evaluated by taking surgical pathology as standard. Results: Ultrasonography of 158 patients (158 lesions) with hepatic cystic echinococcosis showed that there were 19, 37, 23, 9, 58 and 12 lesions of CE1, CE2, CE3a, CE3b, CE4 and CE5 types, respectively. With surgical pathology as standard, the accuracy rates of ultrasound in diagnosing CE1, CE2, CE3a, CE3b, CE4 and CE5 types were 90.00% (18/20), 100% (35/35), 95.83% (23/24), 81.82% (9/11), 98.21% (55/56) and 100% (12/12), respectively. The overall accuracy of ultrasound diagnosis of cystic echinococcosis disease typing was 96.20 per cent (152/158). The detection rates of vascular involvements in different parts by ultrasound were lower than those by surgical pathology (P<0.05). The detection rates of bile duct involvements by ultrasound were also lower than those by surgical pathology (P<0.05). Conclusion: The ultrasonographic manifestations of hepatic cystic echinococcosis have obvious characteristics. The diagnosis of disease typing is consistent with the results of surgical pathology, but it has certain limitations in the diagnosis of vascular and bile duct involvements. 2025 Vol. 31 (12): 2103-2107 [Abstract] ( 132 ) HTML (1 KB)  PDF (1159 KB)  ( 3 ) 2107 Clinical Efficacy of Self-Formulated Wind-Dispelling Decoction Combined with Cinnarizine in Treating Chronic Urticaria and Its Effects on Inflammatory Cytokine Levels ZHANG Weiguang, CUI Jing, ZHANG Dongxu, et al DOI: 10.3969/j.issn.1006-6233.2025.12.029 Objective: To investigate the clinical efficacy of a self-formulated wind-dispelling decoction combined with cinnarizine in treating chronic urticaria and its effect on inflammatory factor levels. Methods: A total of 120 patients with chronic urticaria admitted to our hospital from January 2020 to October 2024 were randomly assigned to the monotherapy group (60 patients, cetirizine hydrochloride tablets) and the combination group (60 patients, wind-dispelling decoction + cinnarizine tablets). Pre- and post-treatment comparisons were made between groups for Traditional Chinese Medicine symptom scores, Dermatology Life Quality Index (DLQI), overall response rate, recurrence rate, and adverse reactions. Serum levels of interleukin-4 (IL-4), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), and IgE were measured for comparison. Results: Pre-treatment DLQI scores showed no statistically significant difference between groups (P>0.05); Post-treatment DLQI scores were significantly lower in the combination group than in the monotherapy group (P<0.05). Pre-treatment TCM symptom scores showed no statistically significant difference between groups (P>0.05), but the change in TCM symptom scores from baseline to post-treatment was greater in the combination group than in the monotherapy group (P<0.05). Pre-treatment inflammatory cytokine levels showed no significant difference between groups (P>0.05). The difference in IL-4, IL-6, TNF-α, and IgE levels before and after treatment was greater in the combination group than in the monotherapy group, with statistically significant differences (P<0.05). The overall response rate in the combination group (93.3%) was higher than that in the monotherapy group (76.7%), and the recurrence rate was lower in the combination group (P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: The combination of wind-dispelling decoction and cinnarizine effectively alleviates symptoms and reduces recurrence rates in chronic urticaria, potentially through modulation of inflammatory factors. 2025 Vol. 31 (12): 2107-2112 [Abstract] ( 129 ) HTML (1 KB)  PDF (1169 KB)  ( 12 )

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