Abstract：Objective: To explore the impacts of microRNA-208b-3p (miR-208b-3p) on the proliferation, invasion, and epithelial mesenchymal transition of non-small cell lung cancer cells by regulating sex determining region Y-box 4 (SOX4). Methods: Cancer tissues and adjacent tissues were collected from non-small cell lung cancer patients, and human non-small cell lung cancer cells A549, H1299, H460 and human normal lung epithelial cells 16HBE were cultured. The qRT-PCR method was used to measure miR-208b-3p and SOX4 mRNA in the aforementioned tissues and cells. H1299 cells were grouped into control group, miR-NC group, miR-208b-3p group, miR-208b-3p+pcDNA group, and miR-208b-3p+SOX4 group. CCK-8 method and clone formation assay were used to measure cell proliferation. Transwell experiment was used to measure cell invasion. Flow cytometry was used to measure cell apoptosis. In addition, western blot was used to measure the SOX4, Bax, Bcl-2, E-cadherin, and Vimentin proteins in cells. Results: Compared with adjacent tissues, miR-208b-3p was decreased and SOX4 mRNA was increased in lung cancer tissues (P<0.05). Compared with 16HBE cells, miR-208b-3p decreased in A549, H1299, and H460 cells, while SOX4 mRNA increased (P<0.05). Compared with the miR-NC group, the miR-208b-3p group showed increases in miR-208b-3p, apoptosis rate, Bax and E-cadherin proteins in H1299 cells, and decreases in SOX4 mRNA and protein, OD value, clone formation rate, Bcl-2 and Vimentin proteins, and number of invasive cells (P<0.05). Compared with the miR-208b-3p+pcDNA group, the miR-208b-3p+SOX4 group showed decreased apoptosis rate, Bax and E-cadherin proteins in H1299 cells, and an increase in SOX4 mRNA and protein, OD value, clone formation rate, Bcl-2 and Vimentin proteins, and number of invasive cells (P<0.05). Conclusion: MiR-208b-3p may inhibit the proliferation, invasion, and epithelial mesenchymal transition of non-small cell lung cancer cells by targeting the inhibition of SOX4.
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2025, Vol. 31 
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