不同程度支气管哮喘患儿血清miR-155 miR-126和miR-125b表达水平及其与气道炎症重塑的相关性研究

doi:10.3969/j.issn.1006-6233.2025.01.020

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Abstract Objective: To analyze the expression levels of serum microRNA-155 (miR-155), microRNA-126 (miR-126), and microRNA-125b (miR-125b) in children with different degrees of bronchial asthma and their correlation with airway inflammation and remodeling. Methods: A total of 108 children with bronchial asthma (disease group) were enrolled in this study from August 2020 to November 2023. Based on the severity of asthma, the children were divided into mild group (63 cases) and severe group (45 cases). Additionally, 50 healthy children with normal physical examinations (control group) were included. The relative expression levels of serum miR-155, miR-126, and miR-125b, as well as airway inflammatory indicators including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and eosinophil cationic protein (ECP), were compared among the severe group, mild group, and control group. Airway remodeling was assessed, including bronchial thickness (T/D) and the ratio of wall area to total cross-sectional area (WA). Lung function indicators such as forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and the ratio of forced expiratory volume in one second to forced vital capacity (FEV1/FVC) were measured. Pearson correlation analysis was used to analyze the correlation between the expressions of serum miR-155, miR-126, and miR-125b and airway inflammation, airway remodeling, and lung function. Receiver operating characteristic (ROC) curve analysis was applied to evaluate the diagnostic value of miRNAs in distinguishing different severities of bronchial asthma in children. Results: The expression levels of serum miR-155, miR-126, and miR-125b were higher in the severe group than in the mild group and control group (P<0.05). Compared with the control group, the levels of serum IL-6, TNF-α, and ECP were significantly higher in both the severe and mild groups (P<0.05). The levels of inflammatory factors (IL-6, TNF-α, ECP) were higher in the severe group than in the mild group and control group (P<0.05). In terms of airway remodeling indicators, T/D and WA were also higher in the severe group than in the mild group and control group (P<0.05). Lung function indicators (FEV1, FVC, FEV1/FVC) were lower in the severe group than in the mild group and control group (P<0.05). Pearson correlation analysis showed that the expression levels of serum miR-155, miR-126, and miR-125b were positively correlated with IL-6, TNF-α, ECP, T/D, and WA, and negatively correlated with FEV1, FVC, and FEV1/FVC (P<0.05). ROC curve analysis revealed that the areas under the curves (AUCs) for serum miR-155, miR-126, miR-125b alone and in combination in predicting the severity of asthma in children were 0.837, 0.817, 0.801, and 0.958, respectively (P<0.05). Conclusion: The high expressions of serum miR-155, miR-126, and miR-125b in children with bronchial asthma are closely related to airway inflammation and remodeling, and have high diagnostic efficiency for assessing the severity of bronchial asthma in children.

Key words： Bronchial asthma MicroRNA-155 MicroRNA-126 MicroRNA-125b Airway inflammation Airway remodeling

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.01.020 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I1/111

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