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2025 Vol. 31, No. 4 Published: 2025-04-30 529 Impacts of LncRNA SNHG1 on Migration Invasion and Chemotherapy Sensitivity of Gastric Cancer Cells by Regulating the miR-330-5p/HMGA2 Axis HE Yiming, JIN Ailian, ZHOU Yipei, et al DOI: 10.3969/j.issn.1006-6233.2025.04.01 Objective: To study the impacts of long non-coding RNA small nucleolar RNA host gene 1 (LncRNA SNHG1) on the migration,invasion and chemotherapy sensitivity of gastric cancer cells by regulating miR-330-5p/high mobility group A2 (HMGA2) axis. Methods: MGC-803 cells were cultured in vitro and randomly grouped into control group,si-SNHG1 group,pc-NC+miR-330-5p-NC group,and si-SNHG1+miR-330-5p inhibitor group.After grouping and transfection,real-time fluorescence quantitative PCR and immunoblotting were performed to detect the expression of LncRNA SNHG1,miR-330-5p,and HMGA2 in each group; cell scratch and Transwell invasion test were performed to detect cell migration and invasion in each group.MGC-803/oxaliplatin (OXA) cells were cultured in vitro and randomly grouped into control group,OXA group,OXA+si-SNHG1 group,OXA+pc-NC+miR-330-5p-NC group,and OXA+si-SNHG1+miR-330-5p inhibitor group.After grouping and transfection,real-time fluorescence quantitative PCR and immunoblotting were performed to detect the expression of LncRNA SNHG1,miR-330-5p,and HMGA2 in each group; CCK-8,Edu staining,and TUNEL staining experiments were conducted to detect cell proliferation and apoptosis in each group.The MGC-803/OXA transplanted tumor nude mouse model was constructed and randomly grouped into a control group,OXA group,OXA+si-SNHG1 group,OXA+pc-NC+miR-330-5p-NC group,and OXA+si-SNHG1+miR-330-5p inhibitor group.After grouping and processing,the tumor volume and weight were measured.Targeted validation was performed using dual luciferase reporter gene assay. Results: Compared with the control group,the expression of LncRNA SNHG1,the expression of HMGA2,migration rate,and invasion number in the si-SNHG1 group decreased (P<0.05),the expression of miR-330-5p increased (P<0.05).Compared with the si-SNHG1 group,the expression of HMGA2,migration rate,and invasion number in the si-SNHG1+miR-330-5p inhibitor group increased (P<0.05),the expression of miR-330-5p decreased (P<0.05).Compared with the control group and OXA group,the expression of LncRNA SNHG1,the expression of HMGA2,cell viability,proliferation rate,tumor volume and weight in the OXA+si-SNHG1 group decreased (P<0.05),the expression of miR-330-5p and apoptosis rate increased (P<0.05); there was no obvious change in all indicators of OXA group cells compared to the control group (P>0.05).Compared with the OXA+si-SNHG1 group,the expression of HMGA2,cell viability,proliferation rate,tumor volume,and weight in the OXA+si-SNHG1+miR-330-5p inhibitor group increased (P<0.05),the expression of miR-330-5p and apoptosis rate decreased (P<0.05).LncRNA SNHG1 was able to target the downregulation of miR-330-5p expression in MGC-803 and MGC-803/OXA cells,and miR-330-5p was able to target the downregulation of their HMGA2 expression. Conclusion: Silencing LncRNA SNHG1 can attenuate the expression of HMGA2 by up-regulating miR-330-5p,thereby inhibiting the migration and invasion of gastric cancer cells,enhancing their chemotherapy sensitivity,and enhancing the killing effect of OXA on them. 2025 Vol. 31 (4): 529-537 [Abstract] ( 40 ) HTML (1 KB)  PDF (2472 KB)  ( 16 ) 537 Effect of miR-653-5p Regulation on the c-Myc/NF-κB Pathway in the Invasion and Metastasis of Endometrial Carcinoma CHEN Hua, JIN Quanfang, HAO Xiaojiao, et al DOI: 10.3969/j.issn.1006-6233.2025.04.02 Objective: To investigate the regulatory effects of miR-653-5p on endometrial cancer and its related mechanisms. Methods: RT-qPCR was used to detect the levels of miR-653-5p in EC tumor tissues and cells.CCK-8,wound healing,and invasion assays were employed to evaluate the viability and metastasis of EC cells.Luciferase reporter gene assays were conducted to confirm the interaction between miR-653-5p and c-Myc.Western blotting was performed to detect the expression levels of c-Myc,p-IκBα,IκBα,p-NF-κB p65,and NF-κB p65 proteins.In vivo animal models were established by implanting EC cells overexpressing miR-653-5p to observe the tumor growth levels. Results: The RT-qPCR results indicated a decreased expression of miR-653-5p in EC tumor tissues and cells.Transfection with miR-653-5p mimics inhibited EC cell proliferation,suppressed cell cycle progression,promoted cell apoptosis,and reduced cell migration.Additionally,c-Myc may be a potential target of miR-653-5p,and overexpression of miR-653-5p altered cell proliferation,migration,and invasion by inhibiting the c-Myc and NF-κB pathways.Transfection with LV-miR-653-5p decreased the protein level of c-Myc and ultimately inhibited the growth of EC in vivo. Conclusion: miR-653-5p inhibits the survival and metastasis of EC cells by suppressing the c-Myc/NF-κB pathways. 2025 Vol. 31 (4): 537-543 [Abstract] ( 26 ) HTML (1 KB)  PDF (2846 KB)  ( 3 ) 543 The Study on the Pathological Mechanisms of LINC01614 in Promoting the Proliferation and Progression of Lung Squamous Cell Carcinoma Cells ZHANG Shilei, XU Chen, WANG Yahong, et al DOI: 10.3969/j.issn.1006-6233.2025.04.03 Objective: To investigate the pathogenesis of LINC01614 in promoting the proliferation and progression of lung squamous cell carcinoma cells. Methods: The relative expression levels of LINC01614 in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LSCC) biopsies (20 cases in each cancer group) and corresponding adjacent tissues (20 cases in each cancer group) were determined by RT-qPCR assay. As well in human immortalized lung epithelial cell line 16HBE cells, human lung squamous cell carcinoma cell line HCC95 cells and NCI-H226 cells, and human lung adenocarcinoma cell line A549 cells and H1299 cells, the relative expression levels of LINC01614 were also determined. Lentivirus-mediated knockdown/overexpression of LINC01614 and infected with HCC95 cells. Cells were divided into the control group, Lenti-shRNA MOCK group, Lenti-LINC01614 shRNA group, Lenti-vector group, and Lenti-LINC01614 OE group. The proliferation abilities of each group of cells were measured by CCK-8 assay. Phosphorylated (p-) PI3K and PI3K, p-Akt, and Akt levels were determined by Western blot. The colony formation abilities were determined by colony formation ability assay. In vivo tumor growth of each group of mice was measured by xenografts. The differences in survival rate of xenografts were measured by survival curve. Results: Compared with LSCC-adjacent tissues, the relative expression levels of LINC01614 in LSCC tissues were increased (P<0.05). Compared with LUAD tissues, the relative expression levels of LINC01614 in LSCC tissues were increased (P<0.05) (F=385.60, P<0.0001). Compared with 16HBE cell group, the relative expression levels of LINC01614 in HCC95 cell group, NCI-H226 cell group, A549 cell group and H1299 cell group were increased (P<0.05); Compared with HCC95 cell group, the relative expression levels of LINC01614 in NCI-H226 cell group, A549 cell group, and H1299 cell group were decreased (P<0.05) (F=347.60, P<0.0001). Compared with the control group, in the Lenti-LINC01614 shRNA group, the cell proliferation ability was decreased (P<0.05). The relative levels of p-PI3K and p-AKT were decreased (P<0.05). Cell colony formation ability decreased (P<0.05). In vivo tumor growth of xenografts was decreased (P<0.05). Compared with the control group, in the Lenti-LINC01614 OE group, the cell proliferation ability was enhanced (P<0.05). The relative levels of p-PI3K and p-AKT were increased (P<0.05); Cell colony formation ability was enhanced (P<0.05). In vivo tumor growth of xenografts was enhanced (P<0.05). The survival rate (%) of xenografts was decreased (Chi square=19.82, P=0.0005). Conclusion: The abnormally high expression of LINC01614 in LSCC tissues could activate the PI3K/Akt signaling pathway and promote the proliferation ability of LSCC cells and LSCC progression. 2025 Vol. 31 (4): 543-549 [Abstract] ( 35 ) HTML (1 KB)  PDF (2511 KB)  ( 5 ) 549 Molecular Mechanisms of HMGB1 in Regulating Ferroptosis to Promote Radioactive Iodine Resistance in Differentiated Thyroid Cancer WUFUER Yimaer, DAERHAN Sailikebieke, DENG Chao, et al DOI: 10.3969/j.issn.1006-6233.2025.04.04 Objective: To explore the possible molecular mechanisms of chronic inflammation in reducing the sensitivity of differentiated thyroid cancer to radioactive iodine therapy. Methods: Surgical resection tissues of patients with differentiated thyroid cancer (DTC tissues,21 cases) or radioiodine (RAI) refractory differentiated thyroid cancer (RR-DTC tissues,13 cases) and corresponding adjacent tissues (Adjacent tissues,34 cases) were included.The mRNA levels of HMGB1,TNF-α,IL-1β and IL-6 were determined by qPCR.The levels of Fe2+ and malondialdehyde (MDA) were determined by chemiluminescent assay.The relative expression levels of TLR4,NIS,p-p38 MAPK,p-PI3K and p-AKT were determined by Western blot.Twenty-four 5-6 weeks old female NOD SCID mice were randomly divided into 4 groups with 6 mice in each group:①K1 group,②K1+ Lienti-NIS KI group,③K1+ Lienti-NIS KI+ Lienti-TLR4 OE group,④K1+ Lienti-NIS KI+ Lienti-vector group.Human papilloma thyroid cancer cell line K1 cells were transfected with lentiviral particles of Lenti-NIS KI to mediate knock-in NIS,or were combined transfected with Lentiviral particles of Lenti-NIS KI + Lenti-TLR4 OE to mediate knockin NIS+ over-expression TLR4.The knock-in control group was Lenti-NIS KI + Lenti-vector group.The K1 cells were injected subcutaneously into the right shoulder back of mice to construct xenografts.Two weeks later,the mice were treated with 3.7 MBq Na131I intravenously.The xenografts' growth was monitored every 2 days.On the 36th day,mice were sacrificed and the tumors were collected.The relative expression levels of TLR4,NIS,HMGB1,p-p38 MAPK,p-PI3K,and p-AKT were determined by Western blot.The levels of Fe2+ and MDA in tumors were determined by chemiluminescent assay. Results: Compared with Adjacent tissues,in DTC tissues,HMGB1,TNF-α,IL-1β,and IL-6 mRNA levels were increased (P<0.05).Fe2+ and MDA levels were increased (P<0.05).The relative expression levels of TLR4,p-p38 MAPK,p-PI3K,and p-AKT were increased (P<0.05).The above indexes were further increased in RR-DTC tissues (P<0.05).The relative expression levels of NIS were decreased in RR-DTC tissues (P<0.05).Compared with K1 group,in K1+ Lenti-NIS KI group,the relative expression levels of NIS and HMGB1,as well as Fe2+ and MDA levels were increased (P<0.05),and the tumor growth rates were significantly inhibited (P<0.05).Compared with K1 + Lenti-NIS KI group,in K1 + Lenti-NIS KI+ Lenti-TLR4 OE group,the relative expression levels of TLR4,p-p38 MAPK,p-PI3K,and p-AKT in tumors were up-regulated,while the relative expression levels of NIS and HMGB1 were decreased.The levels of Fe2+ and MDA were decreased,and the tumor growth rates were significantly increased (P<0.05). Conclusion: DTC up-regulated HMGB1 to inhibit ferroptosis through up-regulation of TLR4 expression,and reduce the sensitivity of DTC to RAI treatment. 2025 Vol. 31 (4): 549-556 [Abstract] ( 17 ) HTML (1 KB)  PDF (1839 KB)  ( 2 ) 556 Exploring the Effect of Resveratrol on UVB-Induced Skin Photoaging Damage in Mice Based on the PI3K/AKT/mTOR Pathway CHEN Ying, ZHAO Juhua, YANG Yu, et al DOI: 10.3969/j.issn.1006-6233.2025.04.05 Objective: To investigate the effect of resveratrol (RES) on ultraviolet B (UVB) induced skin photoaging damage in mice by regulating the phosphoinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. Methods: Mice were assigned into control group,model group,PI3K inhibitor group (LY294002 group),resveratrol group (RES),and resveratrol+PI3K activator group (RES+740 Y-P),with 12 mice in each group; Except for the Control group,mice in all other groups were exposed to UVB radiation.The skin moisture content was measured,oxidative stress (GSH-Px,SOD,MDA) and inflammation (IL-6,TNF-α) related indicators in the skin tissue were measured using ELISA method.The morphological changes in the skin tissue were measured using HE staining,and the collagen fiber content in the skin tissue was measured using Masson staining.The autophagy of skin tissue cells was observed using transmission electron microscopy,the expression of AQP3 and Claudin-1 in skin tissue was measured using immunohistochemistry,and the expression of autophagy (Atg7,LC3-Ⅱ/LC3-I,p62) and PI3K/AKT/mTOR pathway related proteins in the skin tissue was measured using Western blot. Results: Compared with the Control group,the skin moisture content,skin tissue GSH-Px,SOD activities,collagen content,autophagosome count,AQP3,Claudin-1,LC3-Ⅱ/LC3-I,and Atg7 protein levels were lower in the Model group,while the MDA and IL-6,TNF-α levels,epidermal thickness,p62 protein level,the phosphorylation level of signaling pathway protein were higher (P<0.05).Compared with the Model group,the skin moisture content,skin tissue GSH-Px,SOD activities,collagen content,autophagosome count,AQP3,Claudin-1,LC3-Ⅱ/LC3-I,and Atg7 protein levels were higher in the LY294002 group and RES group,while the MDA and IL-6,TNF-α levels,epidermal thickness,p62 protein level,the phosphorylation level of signaling pathway protein were lower (P<0.05).Compared with the RES group,the skin moisture content,skin tissue GSH-Px,SOD activities,collagen content,autophagosome count,AQP3,Claudin-1,LC3-Ⅱ/LC3-I,and Atg7 protein levels were lower in the RES+740 Y-P group.In contrast,the MDA and IL-6,TNF-α levels,epidermal thickness,p62 protein level,the phosphorylation level of signaling pathway protein were higher (P<0.05). Conclusion: RES may improve skin photoaging damage in UVB-irradiated mice by inhibiting the activation of the PI3K/AKT/mTOR signaling pathway,activating autophagy,and reducing oxidative stress and inflammatory response. 2025 Vol. 31 (4): 556-564 [Abstract] ( 19 ) HTML (1 KB)  PDF (1990 KB)  ( 6 ) 564 The Effect of Wenfei Huaxian Decoction on Lung Function and HMGB1/TLR4/NF-κB Signaling Pathway in Pulmonary Fibrosis Model Rats TIAN Hui, FU Taoli, WANG Limei, et al DOI: 10.3969/j.issn.1006-6233.2025.04.06 Objective: To investigate the effects of Wenfei Huaxian decoction (WFHX) on lung function and the high mobility group protein B1 (HMGB1)/Toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling pathway in pulmonary fibrosis (PF) model rats. Methods: Idiopathic pulmonary fibrosis (IPF) was induced in rats using bleomycin (BLM).Successfully modeled rats were divided into the Model group,L-WFHX group (0.18 mg/3mL WFHX),M-WFHX group (0.54mg/3mL WFHX),H-WFHX group (0.9mg/3mL WFHX),and WFHX+LPS group (0.9mg/3mL WFHX + 0.4mg/kg LPS),with 10 rats in each group.Another 10 rats were selected as the Control group (induced with physiological saline).The Model and Control groups were orally gavaged (three times daily) and injected (once daily) with an equal amount of physiological saline for 28 consecutive days.Lung function was assessed in all groups.Lung tissue injury was evaluated using HE staining and Masson staining.Immunohistochemistry was used to detect the expression of α-smooth muscle actin (α-SMA) and collagen I in lung tissue.Enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of transforming growth factor-β1 (TGF-β1) and interleukin-1β (IL-1β) in lung tissue.Western blot was used to detect the expression of HMGB1,TLR4,and NF-κB proteins in rat lung tissue. Results: The Control group showed clear and intact lung tissue structure with no significant inflammatory cell infiltration or collagen fiber deposition.The Model group exhibited significant lung tissue damage,with extensive inflammatory cell infiltration and collagen deposition.The L-WFHX,M-WFHX,and H-WFHX groups showed significant improvement in lung tissue structure,with reduced inflammatory exudation and collagen deposition.The WFHX+LPS group exhibited aggravated lung tissue damage,with increased inflammatory exudation and collagen deposition.Compared to the Control group,the Model group showed higher FEV0.4/FVC,Szapiel score,Ashcroft score,α-SMA,collagen I,TGF-β1,IL-1β,HMGB1,TLR4,and NF-κB expression,and lower FVC and MassFVC (P<0.05).Compared to the Model group,the L-WFHX,M-WFHX,and H-WFHX groups showed lower FEV0.4/FVC,Szapiel score,Ashcroft score,α-SMA,collagen I,TGF-β1,IL-1β,HMGB1,TLR4,and NF-κB expression,and higher FVC and MassFVC (P<0.05).Compared to the H-WFHX group,the WFHX+LPS group showed higher FEV0.4/FVC,Szapiel score,Ashcroft score,α-SMA,collagen I,TGF-β1,IL-1β,HMGB1,TLR4,and NF-κB expression,and lower FVC and MassFVC (P<0.05). Conclusion: WFHX can improve lung function in PF rats,and its mechanism may involve the inhibition of the HMGB1/TLR4/NF-κB signaling pathway. 2025 Vol. 31 (4): 564-570 [Abstract] ( 18 ) HTML (1 KB)  PDF (2590 KB)  ( 6 ) 571 Regulatory Study of miR-223-3p on CaSR Expression and Calcium Oxalate Crystal Adhesion Capacity in Model of Kidney Stones Abudusaimaiti AILI, GUO Mingyue, GUAN Yongzhi, et al DOI: 10.3969/j.issn.1006-6233.2025.04.07 Objective: To investigate the regulatory effects and mechanisms of microRNA-223-3p (miR-223-3p) on the expression of calcium-sensing receptor (CaSR) and the adhesion capacity of calcium oxalate crystals in the formation of calcium oxalate kidney stones. Methods: An in vitro model of calcium oxalate kidney stones was established using rat kidney cells,NRK-52E.The expression levels of CaSR mRNA and protein were assessed using qRT-PCR and Western blot,respectively,while miR-223-3p expression was also evaluated.The target-binding relationship between miR-223-3p and CaSR was validated using a dual-luciferase reporter assay.The effects of overexpressing and silencing miR-223-3p on CaSR expression and calcium oxalate crystal adhesion capacity were analyzed. Results: In the calcium oxalate kidney stone cell model,compared to the control group,the expression of CaSR mRNA and protein was significantly downregulated (P<0.05),while miR-223-3p expression was significantly upregulated (P<0.05).The dual-luciferase reporter assay showed that,compared to the CaSR-WT+miR-NC group,the luciferase activity in the CaSR-WT+miR-223-3p group was significantly decreased (P<0.05),while there was no significant difference in luciferase activity between the CaSR-MT+miR-223-3p and CaSR-MT+miR-NC groups (P>0.05).Under optimal transfection conditions,miR-223-3p overexpression significantly downregulated the levels of CaSR mRNA and protein (both P<0.05),whereas silencing miR-223-3p significantly upregulated their levels (both P<0.05).Compared to the model+miRNA-mimic-NC group,the calcium oxalate crystal adhesion capacity was significantly increased in the model+miR-223-3p-mimic group,while it was significantly decreased in the model+miR-223-3p-inhibitor group (both P<0.05). Conclusion: miR-223-3p targets and inhibits the expression of CaSR,thereby increasing the adhesion capacity of calcium oxalate crystals.Regulating the expression of miR-223-3p may provide new insights for the prevention and treatment of calcium oxalate kidney stones. 2025 Vol. 31 (4): 571-576 [Abstract] ( 12 ) HTML (1 KB)  PDF (2085 KB)  ( 3 ) 577 Exploration of the Effect of Weiling Decoction on Intestinal Barrier Function in Rats with Diarrhea-Type Irritable Bowel Syndrome Based on MLCK/MLC2 Signaling Pathway ZHANG Yanyan, YAN Bo, GUO Husheng, et al DOI: 10.3969/j.issn.1006-6233.2025.04.08 Objective: To observe the effect of Weiling Decoction (WLD) on intestinal barrier function in rats with diarrhea-type irritable bowel syndrome (IBS-D) and to explore its possible mechanism based on the MLCK/MLC2 signaling pathway. Methods: Fifty SD rats were randomly divided into a control group (CON group), a model group (IBS-D group), a pinaverium bromide group (PB group, 0.13mg/kg), a low-dose Weiling Decoction group (WLD-L group, 5.31g/kg), and a high-dose Weiling Decoction group (WLD-H group, 10.62g/kg), with 10 rats in each group. The IBS-D rat model was established using acetic acid enema combined with chronic restraint stress. After successful modeling, the rats in each drug group were administered the corresponding drug by gavage once daily for 14 consecutive days. After the drug administration, the diarrhea index and abdominal wall withdrawal reflex score of rats in each group were detected. Intestinal permeability was assessed using fluorescein isothiocyanate-labeled dextran (FITC-dextran). Histopathological changes in colonic tissue were observed by hematoxylin-eosin (HE) staining. Inflammatory factors (TNF-α, IL-6, IL-1β) in colonic tissue were detected by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the expression levels of tight junction proteins (ZO-1, occludin, claudin-1) and MLCK/MLC2 signaling pathway-related proteins (MLCK, MLC2, p-MLC2) in colonic tissue. Results: Compared with the IBS-D group, the diarrhea index, abdominal wall withdrawal reflex score, intestinal permeability, and levels of inflammatory factors TNF-α, IL-6, and IL-1β were significantly reduced in the PB, WLD-L, and WLD-H groups (P<0.05). The degree of intestinal villus damage in colonic tissue was significantly alleviated, and the expression levels of tight junction proteins ZO-1, occludin, and claudin-1 were significantly increased (P<0.05). Additionally, the expression levels of MLCK/MLC2 signaling pathway-related proteins MLCK and p-MLC2/MLC2 were significantly decreased (P<0.05). The results of the above indicators in the PB and WLD-H groups were superior to those in the WLD-L group (P<0.05). Conclusion: WLD can improve intestinal barrier function in IBS-D rats by inhibiting the MLCK/MLC2 signaling pathway, downregulating the expression of inflammatory factors, and upregulating the expression of tight junction proteins in the intestine. 2025 Vol. 31 (4): 577-582 [Abstract] ( 18 ) HTML (1 KB)  PDF (1578 KB)  ( 5 ) 583 Mechanism of Astragaloside IV in Regulating the GSK-3 β/BMP2 Signaling Pathway on Tooth Movement and Periodontal Tissue in Orthodontic Rats LI Peipei, LOU Huijie, YANG Dapeng DOI: 10.3969/j.issn.1006-6233.2025.04.09 Objective: To investigate the mechanism of astragaloside IV in regulating the GSK-3β/BMP2 signaling pathway on tooth movement and periodontal tissue in orthodontic rats. Methods: Sixty SPF male SD rats were selected,with 18 rats assigned to the blank control group and the remaining 42 rats used to establish orthodontic models.Among the 39 successfully modeled rats,they were randomly divided into a model group (15 rats),an astragaloside IV group (12rats),and an astragaloside IV + CHIR-99021 group (12rats).The blank control group and model group were administered 10mg/kg of 0.9% sodium chloride solution via intragastric gavage and intraperitoneal injection.After successful modeling,the astragaloside IV group received 80mg/kg astragaloside IV via intragastric gavage and 10mL/kg of 0.9% sodium chloride solution via intraperitoneal injection.The astragaloside IV + CHIR-99021 group received 80mg/kg astragaloside IV and 30mg/kg CHIR-99021 via intragastric gavage.Periodontal histopathological changes,alveolar bone resorption,serum inflammatory factors,osteoclast count,periodontal index,tooth movement distance,alveolar bone injury-related indicators,serum oxidative stress markers,and GSK-3β/BMP2 signaling pathway-related protein expression in periodontal tissue were compared among the groups. Results: Compared with the blank control group,the astragaloside IV + CHIR-99021 group and model group showed higher levels of IL-17,reactive oxygen species,TNF-α,PD,IL-6,malondialdehyde,osteoclast count,PLI,alveolar bone resorption,and SBI,while BMP2,Tb.Th,SOD,GSK-3β,BV/TV,tooth movement distance (7d,14d,21d),Tb.N,and glutathione levels were lower (P<0.05).Compared with the model group,the astragaloside IV group showed reduced levels of IL-17,malondialdehyde,PD,reactive oxygen species,IL-6,alveolar bone resorption,PLI,TNF-α,osteoclast count,and SBI,while Tb.Th,GSK-3β,BV/TV,SOD,BMP2,Tb.N,tooth movement distance (7d,14d,21d),and glutathione levels were increased (P<0.05).Compared with the astragaloside IV group,the astragaloside IV + CHIR-99021 group showed decreased Tb.Th,glutathione,BMP2,SOD,tooth movement distance (7d,14d,21d),BV/TV,GSK-3β,and Tb.N,while PD,IL-17,osteoclast count,reactive oxygen species,PLI,TNF-α,alveolar bone resorption,malondialdehyde,IL-6,and SBI levels were increased (P<0.05). Conclusion: Astragaloside IV can inhibit oxidative stress and alveolar bone resorption,reduce osteoclast formation,and promote tooth movement in rats,potentially through activation of the GSK-3β/BMP2 signaling pathway. 2025 Vol. 31 (4): 583-591 [Abstract] ( 14 ) HTML (1 KB)  PDF (1684 KB)  ( 5 ) 591 Exploring the Osteoprotective Effect of Curcumin on Osteoarthritis Model Rats Based on the Ferroptosis Pathway LI Yang, LU Tan, et al DOI: 10.3969/j.issn.1006-6233.2025.04.010 Objective: To explore the osteoprotective effect of curcumin on osteoarthritis (OA) model rats based on the ferroptosis pathway. Methods: Chondrocytes were isolated and cultured from 6-8-week-old SD rats.The cells were divided into the control group,the model group (LPS group),the extremely low-dose curcumin group (1μmoL/L),the low-dose curcumin group (10μmoL/L),the medium-dose curcumin group (20μmoL/L),the high-dose curcumin group (40μmoL/L),and the ferroptosis inhibitor ferrostatin-1 (Fer-1) group.Except for the control group,primary chondrocytes in all other groups were treated with lipopolysaccharide (LPS) to establish OA cell models.Cells in the curcumin groups were treated with corresponding doses of curcumin,while cells in the Fer-1 group were treated with 1μM Fer-1.After 48 hours,cell morphology and damage were observed using toluidine blue staining.Cell apoptosis was detected using the TUNEL method.Cell viability was assessed using the CCK-8 assay.The levels of reactive oxygen species (ROS) and malondialdehyde (MDA) were measured using reagent kits.Western blot (WB) was used to detect the protein expression of acyl-CoA synthetase long-chain family member 4 (ACSL4) and glutathione peroxidase 4 (GPX4).Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the mRNA expression levels of GPX4,heat shock protein B1 (HSPB1),and telomere repeat binding factor 1 (TRF1). Results: Compared with the control group,the LPS group showed a reduced number of chondrocytes,damaged cell morphology,smaller cell nuclei,increased apoptosis rate,elevated levels of MDA and ROS,upregulated ACSL4 protein and TRF1 mRNA expression,and decreased cell viability,GPX4 protein,and GPX4 and HSPB1 mRNA expression (P<0.001).Compared with the LPS group,the low-dose,medium-dose,and high-dose curcumin groups and the Fer-1 group exhibited improved chondrocyte injury,reduced apoptosis rate,restored cell viability,decreased levels of MDA,ROS,ACSL4 protein,and TRF1 mRNA expression,and increased GPX4 protein and GPX4 and HSPB1 mRNA expression (P<0.05). Conclusion: Curcumin can effectively reverse LPS-induced damage to OA chondrocytes.The mechanism may be related to improving cellular antioxidant activity,regulating the expression of ferroptosis-related genes (ACSL4,GPX4,HSPB1,and TRF1),and inhibiting chondrocyte ferroptosis. 2025 Vol. 31 (4): 591-597 [Abstract] ( 18 ) HTML (1 KB)  PDF (2515 KB)  ( 6 ) 597 Exploring the Protective Effect of Clofibrate on Chronic Renal Failure Rats Based on the PPARα/CPT1A Pathway LIU Lixia, ZHANG Lu, QI Shanshan DOI: 10.3969/j.issn.1006-6233.2025.04.011 Objective: To investigate the protective effect of clofibrate on chronic renal failure (CRF) rats based on the PPARα/CPT1A pathway. Methods: Sprague-Dawley male rats were randomly assigned into sham surgery (Sham) group,CRF group,clofibrate group,and clofibrate+GW6471 (PPARα inhibitor) group,with 12 rats in each group.A CRF rat model was constructed using 5/6 nephrectomy.After 8 weeks of corresponding drug intervention,the levels of serum SCr,BUN and blood lipid were detected.biochemical method was applied to detect the activities of SOD,GSH-Px,and content of MDA in renal tissue.ELISA method was applied to detect the levels of tumor necrosis factor-α (TNF-α),interleukin-1β,and IL-6 in renal tissue.HE staining was applied to observe pathological changes in kidney tissue.Masson staining was applied to observe renal interstitial fibrosis injury.Western blot was applied to detect the expression of PPARα and CPT1A proteins in the kidneys. Results: Treatment with clofibrate decreased the levels of SCr,BUN,TC,TG,LDL-C in serum,the content of MDA,levels of TNF-α,IL-1β,IL-6,and proportion of interstitial fibrosis area in renal tissue (P<0.05),increased the level of HDL-C in serum,the activities of SOD and GSH-Px,and levels of PPARα and CPT1A proteins in renal tissue (P<0.05).GW6471 could weaken the protective effect of clofibrate on renal injury in CRF rats (P<0.05). Conclusion: Clofibrate may improve lipid metabolism,inhibit oxidative stress and inflammatory response,and alleviate renal injury in CRF rats by activating the PPARα/CPT1A pathway. 2025 Vol. 31 (4): 597-602 [Abstract] ( 12 ) HTML (1 KB)  PDF (1477 KB)  ( 5 ) 602 Effect of Jianpihuashi Granule and Its Different Components on Intestinal Barrier Substance P and Neurokinin 1 Receptor in IBS-D Rats PAN Tingting, LI Xifeng, LIU Yuling, et al DOI: 10.3969/j.issn.1006-6233.2025.04.012 Objective: To investigate the mechanisms of Jianpihuashi granule and its different components in treating diarrhea-predominant irritable bowel syndrome (IBS-D) with liver-stagnation and spleen-deficiency syndrome by examining colonic mucosal barrier function,substance P (SP),and neurokinin 1 receptor (NK1R). Methods: Ninety Sprague-Dawley rats were randomly divided into nine groups (n=10 per group):normal,model,pinaverium bromide,low-dose Jianpihuashi granule,medium-dose Jianpihuashi granule,high-dose Jianpihuashi granule,Buyi,Qushi,and Liqi groups.During the modeling phase,all experimental groups except the normal group were subjected to Senna leaf administration and restraint stress to simulate the pathological state of IBS-D.Subsequently,all groups except the normal and model groups received respective drug interventions for 14 days.Serum levels of endotoxin,D-lactic acid,and diamine oxidase (DAO) were measured using commercial kits,while SP and NK1R protein expressions were assessed via immunohistochemistry. Results: Compared to the normal group,the model group exhibited significantly elevated serum levels of endotoxin,D-lactic acid,and DAO (P<0.01).After treatment,the low-,medium-,and high-dose Jianpihuashi granule groups showed improved intestinal mucosal barrier function,with stronger effects on endotoxin and DAO levels compared to the pinaverium bromide group.The expression of SP and NK1R proteins in the model group was significantly lower than that in the normal group (P<0.01).Compared with the model group,the pinaverium bromide group showed increased SP protein expression and decreased NK1R protein expression (P<0.01),while the low-,medium-,and high-dose Jianpihuashi granule groups exhibited decreased SP and NK1R protein expression to varying degrees.The SP and NK1R protein expression levels in the Jianpihuashi granule groups were lower than those in the pinaverium bromide group (P<0.01).The Buyi,Qushi,and Liqi groups showed levels of endotoxin,DAO,SP,and NK1R related to the high-dose group,but the underlying mechanism remains unclear. Conclusion: Jianpihuashi granule improves intestinal mucosal barrier function and reduces endotoxin,D-lactic acid,and DAO levels,likely through downregulating SP and NK1R protein expression in the colon. 2025 Vol. 31 (4): 602-608 [Abstract] ( 12 ) HTML (1 KB)  PDF (2167 KB)  ( 6 ) 609 Influence of Sacral Neuromodulation Adjuvant Therapy on Urodynamics and Urinary Tract Infection in Patients with Neurogenic Bladder after Spinal Cord Injury BAI Xiaojing, MA Yafang, CHEN Meng DOI: 10.3969/j.issn.1006-6233.2025.04.013 Objective: To analyze the effect of sacral neuromodulation adjuvant therapy on neurogenic bladder (NB) after spinal cord injury (SCI). Methods: Totally 112 patients with NB after SCI in the hospital from March 2021 to March 2024 were divided into a control group (n=56, conventional treatment) and a treatment group (n=56, sacral neuromodulation adjuvant therapy + conventional treatment) using simple random sampling method. The urodynamic indexes, urination function, occurrence of urinary tract infection (UTI), and quality of life in the two groups were evaluated. Results: After treatment, the residual urine volume in the treatment group was decreased while the maximum bladder capacity and maximum urinary flow rate were increased compared with those in the control group (P<0.05). After treatment, compared with the control group, the daily average urination frequency and daily average urinary incontinence frequency were reduced while the single urination volume was enhanced in the treatment group (P<0.05). The incidence of UTI declined significantly in the treatment group compared to the control group (P<0.05). The scores of dimensions of General Quality of Life Inventory-74 (GQOLI-74) in the treatment group after treatment were higher than those in the control group (P<0.05). Conclusion: The application of sacral neuromodulation adjuvant therapy can improve the urodynamics and urination function, reduce the UTI, and strengthen the quality of life in patients with NB after SCI. 2025 Vol. 31 (4): 609-613 [Abstract] ( 13 ) HTML (1 KB)  PDF (1167 KB)  ( 3 ) 614 Expression and Prognostic Value of Caveolin-3 and TLR4 in Serum of Elderly Patients with Heart Failure Complicated by Arrhythmia KE Chunfeng, CAO Yi, TANG Yan, et al DOI: 10.3969/j.issn.1006-6233.2025.04.014 Objective: To investigate the expression and prognostic value of caveolin-3 and Toll-like receptor 4 (TLR4) in the serum of elderly patients with heart failure (HF) complicated by arrhythmia. Methods: A total of 96 patients with heart failure complicated by arrhythmia admitted to our hospital from June 2022 to June 2023 were included as the study group, and 98 patients without heart failure complicated by arrhythmia were included as the control group. Adverse cardiovascular events that occurred during the 12-month follow-up period were recorded, and the patients were assigned to a poor prognosis group (n=33) and a good prognosis group (n=63) based on the results. Enzyme linked immunosorbent assay was applied to detect the expression levels of Caveolin-3 and TLR4 in serum. Multivariate logistic regression was applied to analyze the factors affecting the prognosis of patients with heart failure complicated by arrhythmia. The receiver operating characteristic (ROC) curve was plotted to analyze the predictive value of Caveolin-3 and TLR4 levels for poor prognosis in patients with heart failure complicated by arrhythmia. Results: Compared with the control group, the serum TLR4 level was higher and Caveolin-3 level was lower in the study group (t=8.393, 10.228, all P<0.05). The difference in cardiac function grading between the poor prognosis group and the good prognosis group was statistically obvious (χ2=35.024, P<0.05). The serum TLR4 level in the poor prognosis group was higher than that in the good prognosis group, while the Caveolin-3 and LVEF levels were lower than those in the good prognosis group (t=6.510, 7.280, 3.816, all P<0.05). Multivariate logistic regression analysis showed that cardiac function grading and TLR4 level were risk factors for poor prognosis, while LVEF and Caveolin-3 levels were protective factors for poor prognosis (P<0.05). ROC curve showed that the AUC of predicting poor prognosis in patients with heart failure complicated by arrhythmia by serum Caveolin-3 and TLR4 levels was 0.775 and 0.854, respectively. The AUC predicted by the combination of the two was 0.898, higher than that of single indicator detection (Z=2.964, P=0.003, Z=2.111, P=0.035). Conclusion: The serum level of Caveolin-3 is decreased and TLR4 is increased in patients with heart failure complicated by arrhythmia. The combined detection of the two can be used as serum markers to evaluate the poor prognosis of patients with heart failure complicated by arrhythmia. 2025 Vol. 31 (4): 614-619 [Abstract] ( 13 ) HTML (1 KB)  PDF (1355 KB)  ( 6 ) 619 Study on the Anti-Inflammatory and Liver Function Protective Effects of Low-Dose Dexamethasone Assisted TACE in the Treatment of Primary Liver Cancer MA Liwei, JIA Zhiqiang, CHEN Wusheng, et al DOI: 10.3969/j.issn.1006-6233.2025.04.015 Objective: To explore the anti-inflammatory and liver function protective effects of low-dose dexamethasone-assisted hepatic artery chemoembolization (TACE) in the treatment of primary liver cancer (PLC). Methods: A total of 256 patients with PLC treated in our hospital from January 2020 to September 2024 were randomly divided into a control group (n=128, TACE treatment) and a study group (n=128, low-dose dexamethasone assisted TACE treatment) using a block randomization method. The inflammatory factors, liver function, tumor markers, and efficacy of the two groups were compared. Results: Compared with preoperative levels, the postoperative levels of tumor necrosis factor (TNF) - α, C-reactive protein (CRP), and interleukin-6 (IL-6) increased in both groups 7days after surgery (P<0.05), and the levels of IL-2 decreased in both groups 7days after surgery (P<0.05). Compared with the control group, the study group had lower differences in TNF - α, CRP, IL-2, and IL-6 before and after surgery (P<0.05). Compared with preoperative levels, the postoperative levels of direct bilirubin (DBIL), total bilirubin (TBIL), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) increased in both groups 7days after surgery (P<0.05). Compared with the control group, the study group had lower differences in DBIL, TBIL, AST, and ALT before and after surgery (P<0.05). Compared with preoperative levels, the postoperative levels of alpha fetoprotein (AFP) and cytokeratin 19 (CK19) in both groups decreased at 7 days after surgery (P<0.05). Compared with the control group, the study group had higher differences in AFP and CK19 before and after surgery (P<0.05). The objective response rate (DRR) and disease control rate (DCR) of the research group were 75.00% and 88.28%, respectively, which were higher than the control group's 62.50% and 70.31% (P<0.05). Conclusion: Dexamethasone low-dose adjuvant TACE has significant anti-inflammatory and liver function protective effects in PLC treatment, and can reduce tumor marker levels, improving treatment efficacy. 2025 Vol. 31 (4): 619-625 [Abstract] ( 11 ) HTML (1 KB)  PDF (1363 KB)  ( 3 ) 625 Analysis of the Diagnostic Efficacy of Circulating OPN and miR-142-5p in Early Detection of Hepatocellular Carcinoma REN Changrong, LIU Hui, ZHANG Kaiqian, et al DOI: 10.3969/j.issn.1006-6233.2025.04.016 Objective: To investigate the diagnostic value of osteopontin (OPN), miR-142-5p and AFP in primary hepatocellular carcinoma (HCC). Methods: A total of 40 patients with HCC, 30 patients with liver cirrhosis (LC), and 25 patients with chronic hepatitis (CH) in our hospital were selected as the research subjects. The levels of circulating OPN, miR-142-5p, and AFP were measured, and the receiver operating characteristic (ROC) curve of each index for the diagnosis of HCC was plotted. Results: OPN, miR-142-5p, and AFP demonstrated diagnostic efficacy for HCC when using patients with chronic liver diseases (CH + LC) as the benign control group. When they (OPN+miR-142-5p+AFP) were combined, the area under ROC was 0.935, and the sensitivity and specificity were 73.53% and 96.4%. Conclusion: OPN and miR-142-5p are potential biomarkers for the diagnosis of HCC. Their combination with AFP can improve the diagnostic sensitivity. 2025 Vol. 31 (4): 625-629 [Abstract] ( 13 ) HTML (1 KB)  PDF (1226 KB)  ( 4 ) 629 Expression of miR-33a and N-cadherin mRNA in Breast Cancer Tissues and Their Relationship with the Prognosis of Patients Within 5 Years After Surgery FU Qiang, PU Ruifang, WANG Feng, et al DOI: 10.3969/j.issn.1006-6233.2025.04.017 Objective: To explore the expression of microRNA-33a (miR-33a) and N-cadherin mRNA in breast cancer tissues and their relationship with the prognosis of patients within 5 years after surgery. Methods: The breast cancer tissues and adjacent normal tissues of 132 patients with breast cancer who received surgical treatment in the First People's Hospital of Longnan City from April 2017 to August 2019 were obtained.The miR-33a and N-cadherin mRNA expression of tissues was detected by Real-time fluorescence quantitative PCR.After 5 years of follow-up,patients were divided into good prognosis group (survival within 5 years,101 cases) and poor prognosis group (death within 5 years,31 cases).The expression levels of miR-33a and N-cadherin mRNA in cancerous and adjacent normal tissues were compared.The relationship and correlation between the expression of miR-33a and N-cadherin mRNA in breast cancer tissues and clinical pathological characteristics of patients were analyzed.The relationship between miR-33a and N-cadherin mRNA expression of breast cancer tissues and the 5-year survival rate after surgery was analyzed.The clinical pathological characteristics and the miR-33a and N-cadherin mRNA expression levels in good and poor prognosis groups were compared.The factors affecting the poor prognosis of breast cancer patients at 5 years after surgery were analyzed by Cox regression analysis.The ROC curve was plotted to analyze the predictive value of miR-33a and N-cadherin mRNA in breast cancer tissues for poor prognosis of patients within 5 years after surgery. Results: Compared with adjacent normal tissues,the expression level of miR-33a in breast cancer tissues was decreased (0.49±0.08 vs 1.00±0.04),and the expression level of N-cadherin mRNA was increased (4.52±0.71 vs 1.00±0.05) (t=65.511,56.819,all P<0.001).Patients with low expression of miR-33a and high expression of N-cadherin mRNA had a higher proportion of tumor diameter ≥2cm,TNM stage Ⅲ,low tumor differentiation,and lymph node metastasis than those with high expression of miR-33a and low expression of N-cadherin mRNA (all P<0.05).The expression of miR-33a in breast cancer tissues was negatively correlated with N-cadherin mRNA,tumor diameter,TNM stage,lymph node metastasis,and positively correlated with the degree of tumor differentiation (all P<0.05).N-cadherin mRNA was positively correlated with tumor diameter,TNM stage,lymph node metastasis,and negatively correlated with degree of tumor differentiation (all P<0.05).The 5-year overall survival rate of patients with high expression of miR-33a and low expression of N-cadherin mRNA (88.06%,88.41%) was higher than that of patients with low expression of miR-33a and high expression of N-cadherin mRNA (64.62%,63.49%),respectively (all P<0.05).Compared with the good prognosis group,the poor prognosis group had a higher proportion of patients with tumor diameter ≥2cm,TNM stage Ⅲ,low tumor differentiation,lymph node metastasis,higher expression level of N-cadherin mRNA,and lower expression level of miR-33a (all P<0.05).Tumor diameter ≥2cm,TNM stage Ⅲ,low tumor differentiation,lymph node metastasis,low expression of miR-33a and high expression of N-cadherin mRNA are independent risk factors affecting the poor prognosis of breast cancer patients within 5 years after surgery (all P<0.05).Compared with the AUC (0.841,0.860) predicted by miR-33a or N-cadherin mRNA alone,the AUC (0.939) of the combination of the two in predicting the poor prognosis of breast cancer patients at 5 years after surgery was higher (all P<0.05). Conclusion: The expression of miR-33a in breast cancer tissues is low,and the expression of N-cadherin mRNA is high,both of which are related to the clinical pathological characteristics and prognosis of patients within 5 years after surgery,and have a high predictive value for the poor prognosis of breast cancer patients within 5 years after surgery. 2025 Vol. 31 (4): 629-636 [Abstract] ( 13 ) HTML (1 KB)  PDF (1349 KB)  ( 3 ) 636 Analysis of the Relationship Between Intestinal Flora Metabolites Peripheral Neutrophils NETs and Lung Injury and Prognosis in Acute Pancreatitis ZHANG Zhichao, ZHU Xuehe, WANG Qi, et al DOI: 10.3969/j.issn.1006-6233.2025.04.018 Objective: To analyze the relationship between intestinal flora metabolites, neutrophil extracellular traps (NETs), and acute pancreatitis lung injury (AP-ALI) and prognosis. Methods: A total of 150 patients with AP-ALI admitted from May 2022 to May 2024 were established as the observation group and 60 patients with simple acute pancreatitis (AP) were established as the control group during the same period. Feces and peripheral blood samples were collected to detect intestinal flora metabolites (acetic acid, propionic acid, butyric acid) and NETs levels, Logistic regression analysis was used to determine whether intestinal flora metabolites and NETs were related to AP-ALI. According to the prognosis within 90 days, 150 AP-ALI patients were divided into a poor prognosis group and a good prognosis group. Clinical data were collected, Logistic regression analysis was used to screen risk factors affecting the poor prognosis of AP-ALI patients, ROC curve was plotted to evaluate the efficacy of intestinal flora metabolites and NETs in predicting poor prognosis of AP-ALI patients. Results: Compared with the control group, acetic acid, propionic acid, and butyric acid levels in the observation group were lower (P<0.05) and NETs levels were higher (P<0.05). Acetic acid (OR=1.222), propionic acid (OR=1.342), butyric acid (OR=1.229), and NETs (OR=1.282) were closely related to AP-ALI (P<0.05). Compared with the good prognosis group, BMI, diabetes proportion, and procalcitonin, D-lactic acid, and NETs levels in the poor prognosis group were higher, and oxygen saturation, acetic acid, propionic acid, butyric acid levels were lower, with statistically significant difference (P<0.05). BMI (OR=1.252), diabetes mellitus (OR=1.242), oxygen saturation (OR=1.229), procalcitonin (OR=1.305), D-lactic acid (OR=1.304), acetic acid (OR=1.410), propionic acid (OR=1.424), butyric acid (OR=1.404) and NETs (OR=1.442) were independent risk factors for poor prognosis in AP-ALI patients (P<0.05). ROC curve showed that the sensitivity and AUC of intestinal flora metabolites (acetic acid, propionic acid, butyric acid) and NETs in predicting poor prognosis of AP-ALI patients were 90.50% and 0.907 respectively, and the predictive value was good. Conclusion: The abnormal changes of intestinal flora metabolites (acetic acid, propionic acid, butyric acid) and NETs levels in AP-ALI patients are closely related to the occurrence and prognosis of AP-ALI, which provides a reference basis for early recognition of AP-ALI and improvement of prognosis of patients. 2025 Vol. 31 (4): 636-648 [Abstract] ( 10 ) HTML (1 KB)  PDF (1301 KB)  ( 4 ) 642 Exploration of Risk Factors for Death after Aortic Dissection Repair and Construction of a Mortality Prediction Model SONG Ying, LI Minghui, GUO Yaojun, et al DOI: 10.3969/j.issn.1006-6233.2025.04.019 Objective: To explore the risk factors affecting postoperative mortality in patients with Stanford type A acute aortic dissection (ATAAD) and to construct a nomogram prediction model. Methods: A retrospective analysis was conducted on 245 ATAAD patients who underwent surgical treatment at the First Affiliated Hospital with Nanjing Medical University from January 2019 to December 2022,with a maximum follow-up period of 65 months.Clinical data were recorded,and patients were divided into a survival group and a death group based on mortality during follow-up.The COX proportional hazards model was used to screen potential prognostic factors,and a nomogram prediction model was constructed.The predictive value of the model was analyzed using calibration curves and receiver operating characteristic (ROC) curves. Results: After follow-up,46 patients (18.77%) died,and 199 patients (81.22%) survived.The median time to death in the death group was 29 months (follow-up range:0–65 months).Univariate analysis identified 22 variables associated with postoperative mortality in ATAAD patients (P<0.05).After multivariate COX proportional hazards regression analysis,severe aortic valve insufficiency (AI),mesenteric ischemia,cerebral ischemia,postoperative new stroke,and a white blood cell count (WBC) ≥11.59×10<suP>9</suP>/L were independently associated with increased postoperative mortality risk (P<0.05).A nomogram prediction model was constructed based on these five variables,with a C-index of 0.817 (P<0.001).Calibration curves showed good agreement between predicted and actual values.Time-dependent ROC curves demonstrated that the model had good clinical discrimination for 1-year,3-year,and 5-year mortality risks (areas under the curve >0.7). Conclusion: Severe AI,mesenteric ischemia,cerebral ischemia,postoperative new stroke,and high preoperative WBC are independently associated with postoperative mortality risk in ATAAD patients.The nomogram model constructed using these five variables is feasible for predicting mortality. 2025 Vol. 31 (4): 642-647 [Abstract] ( 12 ) HTML (1 KB)  PDF (1384 KB)  ( 4 ) 648 Construction of a Predictive Model for Postoperative Recurrence/Metastasis of Endometrial Carcinoma Based on Baseline Data and Three-Dimensional Ultrasound Along with Empirical Validation HUANG Juan, GAO Jianying, ZHANG Jiao, et al DOI: 10.3969/j.issn.1006-6233.2025.04.020 Objective: To construct a predictive model and empirical validation for postoperative recurrence/metastasis of endometrial carcinoma (EMC) based on baseline data and three-dimensional ultrasound. Methods: A total of 289 patients with EMC who underwent surgical treatment at Linfen People's Hospital,Shanxi Province from January 2020 to June 2022 were selected and divided into a recurrence group and a non-recurrence group according to whether recurrence/metastasis occurred within 2 years after surgery.The clinical data and three-dimensional ultrasound data of the two groups were statistically compared.The Lasso-Logistic regression equation was used to analyze the influencing factors of recurrence/metastasis after EMC,and a nomogram prediction model was constructed and validated. Results: Two years after surgery,71 out of 289 EMC patients had recurrence/metastasis,with a recurrence rate of 24.57% (71/289).The recurrence group had a higher proportion of patients with tumor diameter,FIGO stage,pathological grade,muscle invasion ≥1/2,lymphatic vascular cancer thrombus,and VFI than the non-recurrence group,and a lower proportion of patients with postoperative radiotherapy and RI than the non-recurrence group (P<0.05).The Lasso-Logistic regression equation showed that FIGO stage,pathological grade,muscle invasion,postoperative radiotherapy,lymphatic and vascular cancer thrombus,RI,and VFI were independent factors associated with postoperative recurrence/metastasis (P<0.05).The C-index of the Nomogram prediction model constructed based on the above influencing factors was 0.815.The calibration curve and ROC curve showed that the model had high sensitivity and accuracy,and had good predictive value. Conclusion: The recurrence rate of EMC patients is high two years after surgery,and the independent factors associated with postoperative recurrence/metastasis are FIGO stage,pathological grade,muscle invasion,postoperative radiotherapy,lymphovascular invasion,RI,and VFI.The nomogram model constructed based on these factors has high sensitivity and accuracy,and can accurately identify potential high-risk populations in the early stage for personalized prevention and treatment measures. 2025 Vol. 31 (4): 648-655 [Abstract] ( 15 ) HTML (1 KB)  PDF (2236 KB)  ( 4 ) 656 Effects of Four-Dimensional Balance Acupuncture Combined with Fire Dragon Cupping on Facial Nerve Function and Facial Blood Flow in Patients with Peripheral Facial Paralysis SHU Zhensong, et al DOI: 10.3969/j.issn.1006-6233.2025.04.021 Objective: To investigate the effects of four-dimensional balance acupuncture combined with Fire Dragon cupping on facial nerve function and facial blood flow in patients with peripheral facial paralysis. Methods: A total of 126 patients with peripheral facial paralysis admitted to our hospital from January 2021 to January 2024 were selected and randomly divided into a Fire Dragon cupping group and a combined group,with 63 patients in each group.The Fire Dragon cupping group received Fire Dragon cupping therapy,while the combined group received four-dimensional balance acupuncture in addition to Fire Dragon cupping therapy.Facial nerve function,facial blood flow,quality of life,and social and psychological function were evaluated before treatment (T0) and after treatment (T1).Nerve factor levels and adverse reactions were measured before and after treatment,and a 6-month follow-up (T2) was conducted to assess long-term outcomes. Results: At T1,the House-Brackmann (H-B) score and resistance index (RI) levels were significantly reduced (P<0.05),with the combined group showing lower levels than the Fire Dragon cupping group (P<0.05).The peak systolic velocity (PSV),end-diastolic velocity (EDV),World Health Organization Quality of Life-BREF (WHOQOL-BREF),Facial Disability Index (FDI),nerve growth factor (NGF),glial cell-derived neurotrophic factor (GDNF),and brain-derived neurotrophic factor (BDNF) levels were significantly increased in both groups (P<0.05),with the combined group showing higher levels than the Fire Dragon cupping group (P<0.05).At T2,the H-B score,RI,PSV,EDV,WHOQOL-BREF,FDIP,and FDIS in the combined group were significantly better than those in the Fire Dragon cupping group (P<0.05),although these indicators in both groups decreased compared to T1 (P<0.05).The recurrence rate in the combined group (1.59%) was significantly lower than that in the Fire Dragon cupping group (12.70%) (P<0.05). Conclusion: Four-dimensional balance acupuncture combined with Fire Dragon cupping can improve facial nerve function,promote facial blood flow,enhance quality of life and social psychological function in patients with peripheral facial paralysis,and demonstrates certain safety and long-term efficacy. 2025 Vol. 31 (4): 656-662 [Abstract] ( 17 ) HTML (1 KB)  PDF (1195 KB)  ( 5 ) 662 Exploring the Levels and Clinical Significance of CD64 Index SAA STREM-1 and CRP in Peripheral Blood of Children with Viral and Bacterial Diarrhea Infections LIANG Yin, JING Fangli, TIAN Tian, et al DOI: 10.3969/j.issn.1006-6233.2025.04.022 Objective: To explore the peripheral blood CD64 index and serum amyloid A (SAA),soluble triggering receptor expressed on myeloid cells-1 (STREM-1) and C-reactive protein (CRP) levels and differential diagnostic value in children with infectious diarrhea caused by viral and bacterial infections. Methods: A total of 110 children with infectious diarrhea admitted from September 2022 to September 2024 were included in diarrhea group,and their fecal pathogen infection characteristics were analyzed.According to the types of pathogen infection,they were divided into viral group (viral infectious diarrhea,n=68) and bacterial group (bacterial infectious diarrhea,n=42).The baseline data and clinical indicators,peripheral blood CD64 index and serum SAA,STREM-1 and CRP were compared among the two groups of children.ROC curve was drawn to analyze the diagnostic and differential value of peripheral blood CD64 index,SAA,STREM-1 and CRP levels on the pathogenic infection types in children with infectious diarrhea. Results: Among the 110 children with infectious diarrhea,there were 68 cases (61.82%) of viral infection and 42 cases (38.18%) of bacterial infection.The proportion of watery stool and levels of WBC,IL-6,and PCT in viral group were significantly lower than those in bacterial group (P<0.05) while the proportion of organ injury was significantly higher than that in bacterial group (P<0.05).The CD6 index,SAA,STREM-1,and CRP in bacterial group were significantly higher than those in viral group (P<0.05).Using different pathogen types in children as state variables and CD6 index,SAA,STREM-1 and CRP as test variables,ROC curve results showed that the AUCs of CD6 index,SAA,STREM-1 and CRP in predicting bacterial diarrhea in children were 0.983,0.887,0.982 and 0.954 respectively,95%CI were(0.958~1.000,0.825~0.949,0.961~1.000 and 0.904~1.000). Conclusion: Peripheral blood CD64 index and serum SAA,STREM-1 and CRP levels can effectively distinguish bacterial diarrhea from viral diarrhea,and have reference value for the early differential diagnosis of infectious diarrhea caused by different pathogens. 2025 Vol. 31 (4): 662-667 [Abstract] ( 13 ) HTML (1 KB)  PDF (1307 KB)  ( 5 ) 667 Analysis of Manifestations of Prenatal Ultrasound Screening for Fetal Heart Abnormalities and Chromosomal Karyotype Abnormalities CUI Zhihui, ZHAO Xuliang, WEI Zhuojun, et al DOI: 10.3969/j.issn.1006-6233.2025.04.023 Objective: To analyze the manifestations of prenatal ultrasound screening of fetal heart abnormalities and chromosomal karyotype abnormalities. Methods: The clinical data of 138 pregnant women at 16-32 weeks of pregnancy who were diagnosed with fetal heart malformations by ultrasound screening in the outpatient department of the hospital were retrospectively analyzed from January 2021 to August 2024.The fetal heart ultrasound results and chromosomal karyotype abnormalities manifestations were analyzed. Results: Among the 130 fetuses with abnormal heart manifestations suggested by ultrasound,there were 79 cases (57.25%) of single heart abnormalities,14 cases (10.14%) of single heart abnormalities with extracardiac abnormalities,26 cases (18.84%) of multiple heart abnormalities,15 cases (10.87%) of multiple heart abnormalities with extracardiac abnormalities,and 4 cases (2.90%) of arrhythmia.There were 59 cases (42.75%) with extracardiac abnormalities among 138 fetuses.There were 18 fetuses with abnormal karyotypes in chromosome examination.Among them,there were 7 cases with isolated strong echo of heart as the indication,3 cases with strong echo of heart combined with extracardiac abnormality as the indication,2 cases with double outlets of right ventricle,1 case with single ventricular septal defect,1 case with ventricular septal defect combined with other abnormalities,1 case with tetralogy of Fallot,1 case with right aortic arch,1 case with endocardial cushion defect and case with left ventricular dysplasia.The detection rate of chromosomal abnormalities in fetuses with multiple heart abnormalities complicated with extracardiac abnormalities was higher than that of a single heart abnormality. Conclusion: Prenatal ultrasound screening of fetal heart malformations is closely related to chromosomal karyotype abnormalities,which can provide a reliable basis for subsequent invasive examinations.When the fetus has multiple heart malformations,heart malformations with extracardiac malformations,invasive examinations should be performed as soon as possible to reduce the rate of birth defects. 2025 Vol. 31 (4): 667-672 [Abstract] ( 12 ) HTML (1 KB)  PDF (1187 KB)  ( 7 ) 672 Clinical Efficacy of an Improved Comprehensive Surgical Design for the Treatment of Severe Upper Eyelid Skin Laxity in the Elderly GUO Suyun, WANG Hong DOI: 10.3969/j.issn.1006-6233.2025.04.024 Objective: To investigate the clinical effect of modified comprehensive surgical design in treating severe skin relaxation. Methods: Thirty-four patients with severe laxity of the upper eyelid who underwent parallel-excision blepharoplasty (an improved blepharoplasty method) in our hospital from January 2018 to June 2020 were included as the observation group. A total of 34 patients who underwent conventional incisional blepharoplasty in our hospital from January 2016 to December 2017 were retrospectively selected as the control group. All were aged 55-69. Results: Compared with the traditional design method, the modified comprehensive surgical design method significantly improved the therapeutic effect and patient satisfaction 6 months after operation(P<0.05). Postoperative adverse reactions were lower in the observation group than in the control group(P<0.05). Conclusion: In treating elderly patients with severe upper eyelid relaxation, the modified design method of comprehensive double eyelid surgery is more accurate in the design of skin removal volume and significantly reduces complications, which is worthy of clinical application. 2025 Vol. 31 (4): 672-675 [Abstract] ( 11 ) HTML (1 KB)  PDF (1460 KB)  ( 3 ) 676 Relationship Between Serum HSP70 and TRX-1 Levels and Hp Infection in Patients with Chronic Atrophic Gastritis KANG Xuelan, HU Xiaocui, RU Tong, et al DOI: 10.3969/j.issn.1006-6233.2025.04.025 Objective: To investigate the relationship between changes in serum levels of heat shock protein 70 (HSP70) and thioredoxin 1 (TRX-1) with the progression of chronic atrophic gastritis (CAG) and Helicobacter pylori (Hp) infection. Methods: From April 2022 to June 2024,165 patients with CAG admitted to the Gastroenterology Department of our hospital were included as the research subjects.According to the presence or absence of Hp infection in CAG patients,they were separated into CAG patients with Hp infection (Hp group,n=95) and CAG patients without Hp infection (non-Hp group,n=70).The serum levels of HSP70 and TRX-1 were detected by ELISA.The correlation between HSP70,TRX-1 levels with pepsinogen Ⅰ (PG-Ⅰ),pepsinogen Ⅱ (PG-Ⅱ) (Pearson method),and clinical symptom score (Spearman method) was analyzed.Multivariate logistic regression was applied to analyze the influencing factors of Hp infection.ROC curve was applied to evaluate the diagnostic value of HSP70 and TRX-1 levels for Hp infection. Results: The proportion of people with unreasonable diet,clinical symptom scores,and expression levels of HSP70 and TRX-1 in the Hp group were significantly higher than those in the non-Hp group,while the levels of PG-Ⅰ and PG-Ⅱ were lower than those in the non-Hp group (t=5.163,15.129,9.672,5.632,13.561,8.347,all P<0.05).The levels of HSP70 and TRX-1 were significantly negatively correlated with the levels of PG-I and PG-Ⅱ (r=-0.687,-0.696,-0.742,-0.651,all P<0.05),and significantly positively correlated with clinical symptom scores (r=0.716,0.668,all P<0.05).The expression levels of serum HSP70 and TRX-1 in the mild,moderate,and severe groups increased successively (F=17.986,38.924,all P<0.05).The proportions of patients with moderate to severe infiltration,metaplastic atrophy,moderate to severe inflammatory response,moderate to severe atrophy,and moderate to severe intestinal metaplasia in the Hp group were higher than those in the non-Hp group (P<0.05).PG-I and PG-Ⅱ were protective factors for Hp infection in CAG patients (OR=0.483,0.585,all P<0.05),while HSP70 and TRX-1 were risk factors for Hp infection in CAG patients (OR=4.562,5.138,all P<0.05).The AUCs of HSP70 and TRX-1 levels for diagnosing Hp infection in CAG patients were 0.833 and 0.722,respectively.When the two were combined for diagnosing Hp infection,the AUC increased to 0.861,with the highest sensitivity.The AUC of the combined diagnosis of Hp infection by HSP70 and TRX-1 was higher than that of the single diagnosis by HSP70 (Z=2.034,P<0.05) and TRX-1 (Z=2.847,P<0.05). Conclusion: Serum HSP70 and TRX-1 are upregulated in patients with CAG,they are closely related to the development of the disease and Hp infection,and the combination of the two has a high diagnostic value for Hp infection in CAG patients. 2025 Vol. 31 (4): 676-682 [Abstract] ( 15 ) HTML (1 KB)  PDF (1309 KB)  ( 5 ) 682 Therapeutic Effects and Prognostic Analysis of Different Replacement Fluid l Doses of CVVH in Patients with Sepsis Complicated by AKI WENG Chuanyu, GUO Jinchun, HAO Xiaodan DOI: 10.3969/j.issn.1006-6233.2025.04.026 Objective: To analyze the therapeutic effects and prognosis of different replacement fluid doses of continuous veno-venous hemofiltration (CVVH) in patients with sepsis complicated by acute kidney injury (AKI). Methods: A retrospective analysis was conducted on 122 patients with sepsis complicated by AKI who underwent CVVH treatment from March 2021 to December 2023.Patients were divided into three groups based on replacement fluid dose:low-dose group (n=20,20~35mL·kg-1·h-1),medium-dose group (n=61,35~45mL·kg-1·h-1),and high-dose group (n=41,45~70 mL·kg-1·h-1).The CVVH treatment duration,mechanical ventilation time,inflammatory factors [high-sensitivity C-reactive protein (hs-CRP),procalcitonin (PCT),tumor necrosis factor-α (TNF-α),and interleukin-6 (IL-6)],renal function [blood urea nitrogen (BUN) and serum creatinine (Scr)],and prognostic indicators [Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) score and Sequential Organ Failure Assessment (SOFA) score] were compared among the three groups before and after treatment. Results: There were significant differences in hospital stay and mechanical ventilation time among the three groups (P<0.05),with the high-dose group showing significantly shorter durations than the medium- and low-dose groups (P<0.05).Before treatment,there were no significant differences in BUN,Scr,hs-CRP,PCT,TNF-α,and IL-6 levels among the three groups (P>0.05).After treatment,these indicators decreased in all groups (P<0.05),with greater reductions observed in the high-dose group compared to the medium- and low-dose groups (P<0.05).Before treatment,there were no significant differences in APACHE Ⅱ and SOFA scores among the three groups (P>0.05).After treatment,these scores decreased in all groups (P<0.05),with greater reductions in the high-dose group compared to the medium- and low-dose groups (P<0.05). Conclusion: High-dose replacement fluid CVVH is more effective in treating sepsis complicated by AKI,reducing inflammatory factor levels,and improving renal function and prognosis. 2025 Vol. 31 (4): 682-687 [Abstract] ( 14 ) HTML (1 KB)  PDF (1193 KB)  ( 2 ) 687 The Changes in Coronal Spino-Pelvis Alignment in Patients with Developmental Hip Dysplasia after Total Hip Arthroplasty LUO Jinwei, ZHANG Lichao, WU Di, et al DOI: 10.3969/j.issn.1006-6233.2025.04.027 Objective: To investigate the changes in coronal spino-pelvis alignment after total hip arthroplasty (THA) in patients with developmental dysplasia of the hip (DDH). Methods: Twenty-two DDH patients who underwent THA in our hospital from March 2021 to December 2023 were included,comprising 9 males and 13 females with a mean age of 54.23±10.07 years.Among them,19 cases involved the left side and 3 cases involved the right side.Preoperative and postoperative standing full-length anteroposterior and lateral radiographs of the spine and lower extremities were recorded.The following coronal spino-pelvis parameters were measured:Cobb angle,lower limb length,sacral obliquity,iliac obliquity,L5 obliquity,and CE angle.The correlations between these parameters were analyzed. Results: The lower limb length changed from 83.77±4.33 cm preoperatively to 84.02±1.01 cm postoperatively,with no statistically significant difference (P>0.05).However,the difference in lower limb length compared to the healthy limb showed a statistically significant change (P<0.05).The sacral obliquity increased from 4.38±5.62° preoperatively to 4.62±5.43° postoperatively,with no statistically significant difference (P>0.05).The iliac obliquity decreased from 58.19±3.20° preoperatively to 51.00±3.89° postoperatively,showing a statistically significant difference (P<0.05).The CE angle increased from 19.34±7.17° preoperatively to 56.21±9.10° postoperatively,with a statistically significant difference (P<0.05).The Cobb angle changed from 5.70±4.34° preoperatively to 6.03±3.65° postoperatively,with no statistically significant difference (P>0.05).The L5 obliquity changed from 2.75±1.93° preoperatively to 2.47±1.91° postoperatively,with no statistically significant difference (P>0.05).Sacral obliquity was correlated with iliac obliquity and L5 obliquity (r=0.462,P=0.030; r=0.437,P=0.042). Conclusion: THA improved the limb length discrepancy in DDH patients,corrected the coronal obliquity of the pelvis,and improved the mechanical morphology of the lower lumbar region,which may provide some relief for lower back pain. 2025 Vol. 31 (4): 687-691 [Abstract] ( 17 ) HTML (1 KB)  PDF (1180 KB)  ( 4 ) 691 Effect of Xihuang Capsules Combined with Medroxyprogesterone Acetate on the Prognosis and Related Indicators of Endometrial Cancer Patients Undergoing Chemotherapy ZHANG Li, LI Miaomiao, LIU Lize DOI: 10.3969/j.issn.1006-6233.2025.04.028 Objective: To analyze the effects of Xihuang capsules combined with medroxyprogesterone acetate on serum microRNA (miR)-146a, miR-155 levels, and prognosis in patients with endometrial cancer undergoing chemotherapy. Methods: A total of 132 patients with endometrial cancer undergoing chemotherapy from January 2019 to May 2022 were selected and randomly divided into two groups (n=66 per group). The control group received medroxyprogesterone acetate, while the observation group received Xihuang capsules combined with medroxyprogesterone acetate for 3 months. The efficacy and survival rates of the two groups were compared. Serum levels of tumor markers [human epididymis protein 4 (HE4), carbohydrate antigen 125 (CA125), carcinoembryonic antigen (CEA), and cytokeratin-19 fragment (CYFRA21-1)] were measured using enzyme-linked immunosorbent assay before and after 3 months of treatment. Immune indicators (CD3+, CD4+, CD8+, and CD4+/CD8+) were detected using DxFLEX flow cytometry. The expression levels of oncogenes (c-myc, c-erbB2) and tumor suppressor genes (P16, P53) were determined using PCR. The levels of miR-146a and miR-155 were measured using fluorescence quantitative PCR. Results: The total effective rate in the observation group was significantly higher than that in the control group (65.15% vs 42.42%, χ2=6.858, P=0.009). After treatment, the observation group showed lower levels of HE4, CA125, CEA, and CYFRA21-1 compared to the control group (P<0.05). The observation group also exhibited higher levels of CD4+, CD3+, CD4+/CD8+, and miR-146a, and lower levels of CD8+ and miR-155 compared to the control group (P<0.05). The expression of c-myc and c-erbB2 was lower, while the expression of P16 and P53 was higher in the observation group compared to the control group (P<0.05). The 1-year and 2-year survival rates in the observation group were 98.41% and 87.30%, respectively, significantly higher than those in the control group (83.61% and 63.93%, respectively) (P<0.05). Conclusion: Xihuang capsules combined with medroxyprogesterone acetate effectively reduce tumor markers, enhance immune function, regulate oncogene expression, and improve survival prognosis in patients with endometrial cancer undergoing chemotherapy, demonstrating significant clinical application value. 2025 Vol. 31 (4): 691-696 [Abstract] ( 16 ) HTML (1 KB)  PDF (1469 KB)  ( 3 ) 697 Observation on the Curative Effect of Chaihu Guizhi Decoction in the Treatment of Progressive Vitiligo LIU Yuhang, JIN Qiuyue, LI Yuzhu, et al DOI: 10.3969/j.issn.1006-6233.2025.04.029 Objective: To observe the clinical efficacy of Chaihu Guizhi Decoction combined with 308 nm excimer laser irradiation and topical 0.1% tacrolimus ointment in the treatment of progressive vitiligo. Methods: Seventy patients with progressive vitiligo of liver depression and qi stagnation were randomly divided into a treatment group (34 cases,1 case dropped out) and a control group (33 cases,2 cases dropped out) using SPSSsoftware.Both groups received 308 nm excimer laser irradiation and topical application of 0.1% tacrolimus ointment as baseline treatment.The treatment group was administered Chaihu Guizhi Decoction orally,while the control group received compound glycyrrhizin tablets orally for 12 weeks.Clinical efficacy,traditional Chinese medicine (TCM) symptom scores,Hamilton Depression Scale (HAMD) scores,Self-Rating Anxiety Scale (SAS) scores,Dermatology Life Quality Index (DLQI) scores,and serum levels of interleukin-6 (IL-6) and interferon-γ (IFN-γ) were compared between the two groups. Results: The effective rate in the treatment group was 91.18%,significantly higher than 69.70% in the control group (P<0.05).The treatment group showed lower TCM symptom scores,HAMD scores,SAS scores,DLQI scores,and serum levels of IL-6 and IFN-γ compared to the control group (P<0.05). Conclusion: Chaihu Guizhi Decoction combined with 308 nm excimer laser irradiation and topical 0.1% tacrolimus ointment is effective in treating progressive vitiligo and significantly improves emotional symptoms. 2025 Vol. 31 (4): 697-702 [Abstract] ( 12 ) HTML (1 KB)  PDF (1418 KB)  ( 5 )

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