Home  |  About Us  |  Editorial Board  |  Instruction  |  Subscribe  |  Advertisement  |  Messages  |  Contact Us  |  中文
        Office Online
        Journal
Just Accepted
Current Issue
Archive
Advanced Search
Most Read
Most Download
Email Alert
 
         Download
More...  
 
         Links
More...  
 
 
2025 Vol. 31, No. 8
Published: 2025-08-31
 
1233 Effects of Platycodin D on Cartilage Tissue Injury in Knee Osteoarthritis Rats by Adjusting the AMPK/PINK1/Parkin Signaling Pathway
WANG Ping, LIU Yubing
DOI: 10.3969/j.issn.1006-6233.2025.08.01
Objective: To explore the effect of Platycodin D on cartilage tissue injury in knee osteoarthritis (KOA) rats by adjusting the adenosine monophosphate-activated protein kinase (AMPK)/PTEN induced putative kinase 1 (PINK1)/E3 ubiquitin ligase (Parkin) signaling pathway. Methods: Rats were divided into control (NC) group, model group, Platycodin D low-dose group (Platycodin D-L group), Platycodin D high-dose group (Platycodin D-H group), and Platycodin D-H+AMPK pathway inhibitor (Compound C) group, with 10 rats in each group, HE staining method was used to observe the morphological changes of rat cartilage tissue, and the Mankin score was performed. The reagent kit was used to detect the levels of oxidative damage markers SOD, MDA, and NO in the knee cartilage tissue of KOA rats in each group. The DCFH-DA probe was used to detect reactive oxygen species (ROS). The expression levels of inflammatory factors interleukin (IL)-6, tumor necrosis factor α (TNF-α), interleukin (IL)-1β, and monocyte chemoattractant protein 1 (MCP1) in cartilage tissues of each group were detected by ELISA. The TUNEL staining method was used to detect cell apoptosis in the knee cartilage tissue of KOA rats in each group. Western blot was used to detect Caspase 3, Bcl-2 family K protein (Bak), and AMPK/PINK1/Parkin pathway related proteins in cartilage tissue. Results: Compared with the NC group, in the Model group, the rat knee joint cartilage tissue cells were arranged disorderly, with severe cartilage damage. The Mankin score, levels of MDA, NO, ROS, IL-6, TNF-α, IL-1β, and MCP1, cell apoptosis rate, and protein expression levels of Caspase3 and Bak were significantly increased (P<0.05), while the SOD level and protein expression levels of p-AMPK, p-PINK1, and p-Parkin were significantly decreased (P<0.05). Compared with the Model group, in the Platycodin D-L and Platycodin D-H groups, the knee joint cartilage tissue damage in rats was alleviated. The Mankin score, levels of MDA, NO, ROS, IL-6, TNF-α, IL-1β, and MCP1, cell apoptosis rate, and protein expression levels of Caspase3 and Bak were significantly decreased (P<0.05), while the SOD level and protein expression levels of p-AMPK, p-PINK1, and p-Parkin were significantly increased (P<0.05). In the Platycodin D-H + Compound C group, the alleviating effect of Platycodin D on cartilage tissue damage was reversed (P<0.05). Conclusion: Platycodin D may alleviate cartilage tissue injury in KOA rats by adjusting AMPK/PINK1/Parkin signaling pathway.
2025 Vol. 31 (8): 1233-1239 [Abstract] ( 25 ) HTML (1 KB)  PDF (1662 KB)  ( 12 )
1239 The Effects of Crocin on Inflammatory Response and Fibrosis in Elderly Rats with Liver Cirrhosis by Regulating Nrf2/HO-1 Signaling Pathway
HU Fang, ZHAO Feiyan, SONG Rujun, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.02
Objective: To explore the effects of crocin (CR) on inflammation and fibrosis in elderly rats with liver cirrhosis by regulating the nuclear factor E2 related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. Methods: Rat model of liver cirrhosis was established by subcutaneous injection of carbon tetrachloride. The successfully modeled rats were assigned into a model group, L-CR group, M-CR group, H-CR group (intraperitoneal injection of 20, 40, and 80mg/kg CR), and ML385 group (intraperitoneal injection of 80mg/kg CR+intraperitoneal injection of 30mg/kg Nrf2/HO-1 signaling pathway inhibitor ML385), each with 10 rats. In addition, 10 normal rats were recruited as the control group. The control group and model group were intraperitoneally injected with an equal amount of physiological saline, once a day for 4 weeks of continuous intervention. Fully automatic biochemical analyzer was used to detect ALT and AST. The reagent kits were used to measure hyaluronic acid (HA), type III procollagen (PCⅢ), type Ⅳ collagen (Ⅳ-C), and laminin (LN). HE staining was used to observe pathological changes in rat liver tissue. Sirius red staining was used to observe changes in collagen deposition in rat liver tissue. ELISA was used to measure the inflammatory and oxidative stress factors in rat serum. Western blot was used to measure the changes of Nrf2/HO-1 signaling pathway related proteins in rat liver tissue. Results: For the control group, the liver tissue of rats in the model group was disordered, with severe collagen deposition and inflammatory cell infiltration. The ALT, AST, HA, PC Ⅲ, Ⅳ-C, LN, TNF-α, IL-6, IL-1β, and MDA increased (P<0.05), while the SOD, Nrf2, and HO-1 decreased (P<0.05). For the model group, the L-CR group, M-CR group, and H-CR group showed neat arrangement of liver tissue, reduced collagen deposition, and inflammatory cell infiltration. The ALT, AST, HA, PCⅢ, Ⅳ-C, LN, TNF-α, IL-6, IL-1β, and MDA reduced clearly (P<0.05), while SOD, Nrf2, and HO-1 increased clearly (P<0.05). For the H-CR group, the liver tissue arrangement of rats in ML385 group was more disordered, with increased collagen deposition and inflammatory cell infiltration. The ALT, AST, HA, PC Ⅲ, Ⅳ-C, LN, TNF-α, IL-6, IL-1β, and MDA increased clearly (P<0.05), while SOD, Nrf2, and HO-1 decreased clearly (P<0.05). Conclusion: CR may inhibit inflammation and fibrosis in cirrhotic rats by activating Nrf2/HO-1 signaling pathway.
2025 Vol. 31 (8): 1239-1245 [Abstract] ( 16 ) HTML (1 KB)  PDF (2042 KB)  ( 9 )
1245 Celastrol Alleviates Pain in Rats with Gouty Arthritis by Promoting Autophagy via the AMPK/mTOR Signaling Pathway
WU Yuheng, LI Huan, GUO Bo, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.03
Objective: To explore the effects of Celastrol on the pain in rats with acute Gouty arthritis (GA), and its mechanism of promoting autophagy through AMPK)/ mTOR signaling pathway. Methods: GA rat model was established by injecting Monosodium urate (MSU) into articular cavity. Totally 60 models were randomly divided into model group, Celastrol low-dose group (1g/kg Celastrol solution by gavage), Celastrol high-dose group (2g/kg Celastrol solution by gavage) and Compound C group (AMPK pathway inhibitor, 2mg/kg Celastrol solution by gavage+15mg/kg Compound C solution by intraperitoneal injection), with 15 rats in each group. Fifteen more SD rats were randomly selected as the control group (equal volume of normal saline was injected into the right posterior ankle joint). After the treatment, the swelling degree of ankle joint of rats in each group was detected by vernier caliper, and the gait and inflammatory index were scored. Von Frey Hair fiber was used to detect the mechanical pain threshold of rats in each group. The levels of substance P (SP) and calcitonin gene-related peptide (CGRP) in rat serum were detected by enzyme-linked immunosorbent assay. HE staining was used to observe the histopathological changes of synovial tissue of ankle joint and score the degree of inflammatory infiltration. Western Blot was used to detect the expression levels of autophagy-related proteins Beclin-1, LC3Ⅱ/LC3Ⅰ and AMPK/mTOR signaling pathway-related proteins in ankle joints. Results: In the model group, the ankle joint structure was severely damaged, synovium proliferated, joint histopathological score, joint swelling, gait score, inflammatory index score, SP, CGRP and p-mTOR increased significantly (P<0.05), while mechanical pain threshold, Beclin-1, LC3Ⅱ/LC3Ⅰ, p-AMPK and p-ULK-1 decreased significantly (P Compared with the model group, the pathological injury of ankle joint of rats in low and high doses of Celastrol and Compound C group was obviously improved, and the joint histopathological score, joint swelling, gait score, inflammatory index score, SP, CGRP and p-mTOR were significantly reduced (P<0.05), and the mechanical pain threshold, Beclin-1, LC3Ⅱ/LC3Ⅰ, p-AMPK and P-MTOR were significantly reduced. Compared with the low-dose Celastrol group, the pathological score of joint tissue, joint swelling, gait score, inflammatory index score, SP, CGRP and p-mTOR in the high-dose Celastrol group and Compound C group decreased significantly (P<0.05), while the mechanical pain threshold, Beclin-1, LC3II/LC3I, p-AMPK and p-ULK-1 increased significantly. Compared with the high-dose Celastrol group, Compound C group reversed this result. Conclusion: Celastrol can inhibit the secretion of pain mediators and improve the pain symptoms of acute GA rats, and its mechanism is related to activating AMPK/mTOR pathway to promote autophagy.
2025 Vol. 31 (8): 1245-1252 [Abstract] ( 15 ) HTML (1 KB)  PDF (1776 KB)  ( 3 )
1252 Effects of Remimazolam on Postoperative Cognitive Dysfunction in Elderly Rats by Modulating NLRP3/Caspase-1/GSDMD Signaling Pathway
LI Mei, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.04
Objective: To investigate the effects of remimazolam (RM) on postoperative cognitive dysfunction (POCD) in elderly rats by modulating NLRP3/caspase-1/GSDMD signaling pathway. Methods: A POCD rat model was constructed, and successfully modeled rats were grouped into POCD group, L-RM, M-RM, H-RM groups (intraperitoneal injection of 10, 15, and 20mg/kg RM), and H-RM+NS group (intraperitoneal injection of 20mg/kg RM+gavage of 4mg/kg NLRP3 activator NSS), each with 10 rats. Another 10 normal rats were designated as Control group (only the abdominal cavity was opened without removing the liver lobe). The Control group and POCD group were given equal amounts of physiological saline, once a day for 4 consecutive weeks. Morris water maze experiment was applied to test cognitive function in rats. Nissl staining was used to measure the number of neurons in hippocampus of rats. TUNEL staining was performed to measure neuronal apoptosis in hippocampus of rats. ELISA was used to test inflammatory factors in hippocampal tissue. Western blot was performed to test changes in proteins related NLRP3/caspase-1/GSDMD signaling pathway in hippocampal tissue. Results: The number of hippocampal neurons in the POCD group rats was reduced compared to the Control group, with atrophy and a decrease in Nissl bodies (P<0.05); the L-RM, M-RM, and H-RM groups showed an increase in the number of Nissl bodies and neurons compared to the POCD group, with significant alleviation of atrophy and cell body shrinkage (P<0.05); the H-RM+NSS group exhibited significant neuronal atrophy and fewer Nissl bodies compared to the H-RM group (P<0.05). The POCD group had fewer number of times the platform than the Control group, and higher apoptosis rate of hippocampal neurons, IL-6, TNF-α in hippocampal tissue, NLRP3, caspase-1, and GSDMD-N proteins than the Control group (P<0.05). Compared with the POCD group, the number of platform crossings was higher in rats of the L-RM, M-RM, and H-RM groups, while the hippocampal neuron apoptosis rate, levels of IL-6 and TNF-α in hippocampal tissues, and protein expressions of NLRP3, caspase-1, and GSDMD-N were lower (P<0.05). In contrast, compared with the H-RM group, the H-RM + NSS group showed fewer platform crossings, a higher hippocampal neuron apoptosis rate, elevated levels of IL-6 and TNF-α in hippocampal tissues, and increased protein expressions of NLRP3, caspase-1, and GSDMD-N (P<0.05). Conclusion: RM can improve cognitive function in elderly POCD rats, which may be achieved by inhibiting NLRP3/caspase-1/GSDMD signaling pathway.
2025 Vol. 31 (8): 1252-1257 [Abstract] ( 12 ) HTML (1 KB)  PDF (1886 KB)  ( 3 )
1258 Impact of Wogonin on Vascular Calcification in Chronic Kidney Disease Rats by Regulating Wnt/β-catenin Signaling Pathway
LIU Ye, SUN Fuyun, TIAN Yi, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.05
Objective: To investigate the impact of wogonin (WOG) on vascular calcification in chronic kidney disease (CKD) rats by regulating the Wnt/β-catenin signaling pathway. Methods: Nine rats were stochastically selected as control group, while the rest were constructed as CKD models. After successful treatment, they were assigned into CKD group, WOG+CKD group, and WOG+CKD+activator group, with nine rats in each group. After 8 weeks of administration, biochemical indicators such as blood urea nitrogen (BUN), creatinine (SCr), serum calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), and whole parathyroid hormone (iPTH) were detected in the serum of rats in each group. Von Kossa staining was performed to detect aortic calcification. Immunohistochemical staining was performed to detect Runt related transcription factor 2 (Runx2) in aortic tissue. RT-qPCR was performed to detect the mRNA expression of Wnt1 and β-catenin in rat aortic tissue. In addition, Western blot was used to detect the expression of Wnt1 and β-catenin proteins in rat aortic tissue. Results: Compared with the control group, CKD group showed increased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, severe aortic calcification, and elevated Wnt1 and β-catenin mRNA and protein expression (P<0.05). Compared with the CKD group, the WOG+CKD group showed decreased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, reduced aortic calcification, and decreased Wnt1 and β-catenin mRNA and protein expression (P<0.05). Compared with the WOG+CKD group, the WOG+CKD+activator group showed increased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, worsened aortic calcification, and elevated Wnt1 and β-catenin mRNA and protein expression (P<0.05). Conclusion: WOG may alleviate vascular calcification in CKD rats by inhibiting the Wnt/β-catenin signaling pathway.
2025 Vol. 31 (8): 1258-1263 [Abstract] ( 10 ) HTML (1 KB)  PDF (1793 KB)  ( 4 )
1263 Effects of Ginsenoside Rg1 Regulating SIRT1/AMPK/PGC1α Signaling Pathway on Adriamycin-Induced Myocardial Injury in Rats
XIE Yuemin, HAN Aizi, CHEN Qian, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.06
Objective: To investigate the protective effect of ginsenoside Rg1 (G-Rg1) on adriamycin-induced myocardial injury in rats. Methods: A total of 75 SPF grade SD male rats were randomly divided into control group, model group, low, high dose G-Rg1 group and high-dose G-Rg1+SIRT1 inhibitor EX-527 group, with 15 rats in each group. The rat myocardial injury model was constructed by intraperitoneal injection of adriamycin. The rats in the low and high dose G-Rg1 groups were given 25 and 50mg/kg G-Rg1, respectively; the rats in the high dose G-Rg1+SIRT1 inhibitor EX-527 group were given 50mg/kg G-Rg1 by gavage and injected with 5μg/kg EX-527 in the tail vein; the control group and the model group were given gavage and intraperitoneally injected with the same amount of normal saline. The cardiac function of rats were detected; the levels of lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), oxidative stress indicator superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of myocardial tissue in rats were observed by hematoxylin-eosin (HE) staining. Apoptosis in rat myocardial tissue was detected by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling (TUNEL) staining. The contents of nicotinamide adenine dinucleotide (NAD+), adenosine monophosphate (AMP) and adenosine triphosphate (ATP) in myocardial tissue were determined by high performance liquid chromatography. The expression of silencing information regulator 1 (SIRT1)/adenylate activated protein kinase (AMPK)/peroxisome proliferators-activated receptor γ coactivator α (PGC1α) pathway-related proteins was detected by Western-Blot. Results: High dose G-Rg1 significantly improve the pathological damage, such as myocardial cell hypertrophy, swelling and deformation, inflammatory cell infiltration (P<0.05). Increased left ventricular ejection fraction, left ventricular shortening fraction, serum SOD level, myocardial NAD+ and ATP, SIRT1, PGC1α protein expression and AMPK phosphorylation level, and decreased left ventricular end diastolic diameter, left ventricular end-systolic diameter, serum LDH , CK , CK-MB, MDA levels, AMP, AMP/ATP and myocardial cell apoptosis rate in adriamycin-induced myocardial injury rats (P<0.05). However, all the above improvement effects of G-Rg1 at high dose were mitigated or inhibited by EX-527 (P<0.05). Conclusion: G-Rg1 may play a role in alleviating adriamycin-induced myocardial injury in rats by activating the SIRT1/AMPK/PGC1α signaling pathway.
2025 Vol. 31 (8): 1263-1269 [Abstract] ( 8 ) HTML (1 KB)  PDF (1827 KB)  ( 3 )
1269 Expression of miR-106b-5p in Colon Cancer Tissues and its Influences on Biological Function of Human Colon Cancer LOVO/HCT116 Cells
MA Hailin, JIANG Sheng, MA Zhiping
DOI: 10.3969/j.issn.1006-6233.2025.08.07
Objective: To explore the expression of miR-106b-5p in colon cancer tissues and its influences on biological function of human colon cancer LOVO/HCT116 cells. Methods: The expression of has-miR-106b-5p in human colon cancer tissues and para-carcinoma tissues was detected by real-time fluorescent quantitative PCR (RT-PCR). Taking colon cancer LOVO/HCT116 cells as the study background, cell lines with miR-106b-5p overexpression and their negative controls (miR-106b-5p mimic group, mimic NC group) were constructed by miR-106b-5p-mimic and mimic NC, and they were verified by RT-PCR. The cells proliferation, cycle distribution, apoptosis, migration and invasion after transfection were detected by CCK8, flow cytometry and Tanswell assay, respectively. The expressions of related proteins were detected by Western blot. Results: The expression of has-miR-106b-5p in human colon cancer tissues was lower than that in para-carcinoma tissues (t=101.720, P<0.001). After transfection with miR-106b-5p, expression of has miR-106b-5p in LOVO/HCT116 cells in miR-106b-5p mimic group was higher than that in mimic-NC group (P<0.05). Compared with mimic group, cell proliferation rates were decreased in miR-106b-5p mimic group at 24, 48, 72, 96 and 120h after transfection (P<0.05), proportion of G0/G1 stage and apoptosis rate were increased, proportion of S stage and number of cells migration and invasion cells were decreased (P<0.05), expressions of vimentin (Vimentin), phosphorylated extracellular regulated kinase 1/2 (p-ERK1/2) and phosphorylated protein kinase B1/2/3 (p-AKT1/2/3) proteins were decreased (P<0.05), and expressions of E-cadherin and cleaved cysteine proteinase 3 (cleaved-caspase3) proteins were increased (P<0.05). Conclusion: The expression of miR-106b-5p is down-regulated in colon cancer tissues. Overexpression of miR-106b-5p can inhibit proliferation, migration and invasion of human colon cancer LOVO/HCT116 cells, and promote apoptosis, which may be related to the regulation of AKT and ERK signaling pathways.
2025 Vol. 31 (8): 1269-1275 [Abstract] ( 10 ) HTML (1 KB)  PDF (1914 KB)  ( 3 )
1275 CircZNF609 Promoting Angiogenesis in Ovarian Cancer Cells by Targeting the miR-150-5p/VEGF Axis
WANG Jie, YANG Fei, ZHAO Fang
DOI: 10.3969/j.issn.1006-6233.2025.08.08
Objective: To investigate the role of circZNF609 in angiogenesis of ovarian cancer cells and its potential mechanism. Methods: The OVCAR3 cells were divided into 10 groups with three replicate wells each: si-NC group (transfected with control si-NC), si-ZNF609 group (transfected with si-ZNF609), NC mimic group (transfected with NC mimic), miR-150-5p mimic group (transfected with miR-150-5p mimic), NC inhibitor group (transfected with NC inhibitor), miR-150-5p inhibitor group (transfected with miR-150-5p inhibitor), si-ZNF609 + NC inhibitor group (cotransfected with si-ZNF609 and NC inhibitor), si-ZNF609 + miR-150-5p inhibitor group (cotransfected with si-ZNF609 and miR-150-5p inhibitor), si-ZNF609 + pcDNA-NC group (cotransfected with si-ZNF609 and pcDNA-NC), and si-ZNF609 + pcDNA-VEGF group (cotransfected with si-ZNF609 and pcDNA-VEGF). RT-qPCR was used to detect the expression levels of ZNF609 and miR-150-5p, while Western blot was employed to measure the expression level of VEGF. Plate cloning and Transwell assays were conducted to assess the proliferation activity and invasion ability of OVCAR3 cells. An in vitro Matrigel tube formation assay was performed to evaluate angiogenesis. Results: Compared with the normal human ovarian epithelial cell line IOSE80, the expression of ZNF609 was significantly increased, while the expression of miR-150-5p was significantly decreased in human ovarian cancer cell lines (P<0.05). Compared with the si-NC group, the si-ZNF609 group exhibited significantly reduced expression of ZNF609 and VEGF, cell clone numbers, transmembrane cell counts, and the number of formed tubules, along with a significant increase in miR-150-5p expression (P<0.05). In comparison with the si-ZNF609 + NC inhibitor group, the si-ZNF609 + miR-150-5p inhibitor group showed significantly increased cell clone numbers, transmembrane cell counts, and formed tubule numbers, as well as a significant decrease in miR-150-5p expression (P<0.05). The miR-150-5p mimic group had significantly lower VEGF expression levels compared with the NC mimic group (P<0.05), whereas the miR-150-5p inhibitor group showed significantly higher VEGF expression levels compared with the NC inhibitor group (P<0.05). When compared with the si-ZNF609 + pcDNA-NC group, the si-ZNF609 + pcDNA-VEGF group exhibited significantly increased VEGF expression levels, cell clone numbers, transmembrane cell counts, and formed tubule numbers (P<0.05). Conclusion: circZNF609 is highly expressed in ovarian cancer, and knockdown of its expression can inhibit angiogenesis in ovarian cancer cells. This mechanism may be related to the regulation of miR-150-5p/VEGF axis by circZNF609.
2025 Vol. 31 (8): 1275-1282 [Abstract] ( 10 ) HTML (1 KB)  PDF (2265 KB)  ( 4 )
1282 The Expression of Zinc α2 Glycoprotein in Temporal Lobe Epilepsy and Its Correlation with CaMK Ⅱ Phosphorylation and HCN
DUAN Xiaomei, XIAO Zijian, YE Qing, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.09
Objective: To explore the expression of Zinc alpha-2 glycoprotein (ZAG) in temporal lobe epilepsy (TLE) and its correlation with CaMKⅡ and HCN channels. Methods: Adeno associated virus vectors (AAV-ZAG and AAV-NC) were injected into the unilateral ventricle of rats. Three weeks later, Pilocarpine hydrochloride (PHCL) was intraperitoneally injected to construct a TLE model. Three days before modeling, CaMKII inhibitor KN-93 was intraperitoneally injected and randomly divided into eight groups: control group, model group, AAV-NC+model group, AAV-ZAG+model group, AAV-NC+saline group, AAV-NC+KN-93 group, AAV-ZAG+saline group, and AAV-ZAG+KN-93 group. Record the time of the first occurrence of grade IV or above epilepsy after injection of PHCL and the number of spontaneous epileptic seizures in the 5th week; Immunofluorescence detection of ZAG and GFAP expression in rat hippocampal tissue; WB detection of the expression levels of ZAG, p-CaMKII, HCN1, and HCN2 in rat hippocampal tissue. Results: The expression of ZAG in the hippocampal tissue of TLE rats was significantly downregulated (P<0.05); overexpression of ZAG in hippocampal tissue can significantly prolong the latency of TLE (P<0.05), reduce the number of seizures (P<0.05), decrease the number of GFAP positive cells (P<0.05), upregulate the expression levels of HCN1, HCN2, and p-CaMKII (P<0.05), but the addition of CaMKII inhibitors can reverse these effects. Conclusion: ZAG regulates HCN channel protein activity by promoting CaMKII phosphorylation, thereby improving TLE.
2025 Vol. 31 (8): 1282-1289 [Abstract] ( 12 ) HTML (1 KB)  PDF (2530 KB)  ( 5 )
1289 The Effects of LncRNA SNHG16 Regulating the miR-93-5p/TXNIP Axis on Cardiomyocyte Apoptosis Induced by High Glucose
HU Yiqiong, YU Yuan, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.010
Objective:To investigate the effect of LncRNA SNHG16 (long non-coding RNA small nucleolar RNA host gene 16) on high glucose induced cardiomyocyte apoptosis by regulating miR-93-5p (microRNA-93-5p)/TXNIP (thioredoxin interacting protein). Methods: The cell model of diabetes cardiomyopathy was constructed in vitro, and qRT-PCR was used to detect the relative expression levels of LncRNA SNHG16 and miR-93-5p. Cardiomyocyte AC16 was assigned into Control group, Model group, sh-NC group, sh-SNHG16 group, sh-SNHG16+inhibitor NC group, and sh-SNHG16+miR-93-5p inhibitor group, miR-NC group, miR-93-5p mimics group, miR-93-5p mimics+pcDNA group, miR-93-5p mimics+TXNIP group. The relative expression levels of LncRNA SNHG16, miR-93-5p and TXNIP mRNA in AC16 cells of each group were detected by qRT-PCR, the cell proliferation ability was detected by CCK-8 kit method, the cell apoptosis was detected by flow cytometry, the levels of oxidative stress factors were detected by ELISA, and Western blotting was used The expression levels of TXNIP and apoptotic proteins in cells were detected by blot method, and the dual-luciferase reporter gene assay was used to verify the targeting relationship between miR-93-5p and LncRNA SNHG16 and TXNIP. Results: Compared with the Control group, the relative expression levels of LncRNA SNHG16 and TXNIP mRNA, apoptosis rate, protein expression levels of TXNIP, Bax, Caspase 3, Cleaved caspase 3, and level of MDA in AC16 cells in the Model group were greatly higher, the cell viability, expression level of miR-93-5p, protein expression level of Bcl-2, and activity of SOD were greatly lower (P<0.05). Silting the expression of LncRNA SNHG16 or overexpression of miR-93-5p inhibited cell apoptosis and oxidative stress by up-regulating the expression of miR-93-5p (P<0.05). Overexpression of miR-93-5p can improve the pathological changes of high-glucose induced AC16 cells by down-regulating the expression of TXNIP (P<0.05). The dual luciferase reporter gene experiment demonstrated the targeting relationship between miR-93-5p with LncRNA SNHG16 and TXNIP. Conclusion: LncRNA SNHG16 is overexpressed in high glucose induced AC16 cells. Silencing LncRNA SNHG16 can sponge miR-93-5p, downregulate TXNIP expression, inhibit AC16 cell oxidative stress and apoptosis, and enhance its activity.
2025 Vol. 31 (8): 1289-1296 [Abstract] ( 10 ) HTML (1 KB)  PDF (2209 KB)  ( 6 )
1297 Impacts of miR-199a-5p on Glycolysis and Collagen Synthesis in Keloid Fibroblasts by Regulating PKM2
LIU Yulan, GONG Lixin, YU Daojiang, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.011
Objective: To explore the impacts of microRNA-199A-5p (miR-199a-5p) on glycolysis and collagen synthesis in keloid fibroblasts (KFB) by regulating pyruvate kinase isozyme type M2 (PKM2). Methods: The dual luciferase reporter gene assay was used to examine the interaction between miR-199a-5p and PKM2. KFB was assigned into Control group, NC-mimics group, miR-199a-5p-mimics group, miR-199a-5p-mimics+OE-NC group, and miR-199a-5p-mimics+OE-PKM2 group. The qRT-PCR method was used to measure miR-199a-5p and PKM2 mRNA in KFB. CCK8 was used to measure KFB proliferation. The reagent kits were used to measure glucose consumption and lactate production. Flow cytometry was used to detect apoptosis of KFB. Western blot was used to detect type I collagen (Col-I), type III collagen (Col-III), and PKM2 protein in KFB. Results: MiR-199a-5p could target negative regulation of PKM2(t = 14.394, P<0.001). The miR-199a-5p-mimics group had higher apoptosis rate and miR-199a-5p, and lower A450 value, glucose consumption, lactate production, PKM2 mRNA and protein, Col-Ⅰ, and Col-Ⅲ than Control group and NC-mimics group (P<0.05). The miR-199a-5p-mimics+OE-PKM2 group had higher A450 value, glucose consumption, lactate production, PKM2 mRNA and protein, Col-Ⅰ, Col-Ⅲ, and lower apoptosis rate than the miR-199a-5p-mimics group and the miR-199a-5p-mimics+OE-NC group (P<0.05). Conclusion: MiR-199a-5p may regulate PKM2 to inhibit glycolysis and collagen synthesis in KFB.
2025 Vol. 31 (8): 1297-1302 [Abstract] ( 10 ) HTML (1 KB)  PDF (1680 KB)  ( 3 )
1302 Association of SYNM GALNT7 and FOXF2 Expression with Clinicopathological Features and Prognosis in Prostate Cancer Tissues
LI Shuhao, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.012
Objective: To investigate the association of tissue-linked mitogenin (SYNM), N-acetylaminogalactosyltransferase 7 (GALNT7), and forkhead box transcription factor F2 (FOXF2) expression with clinicopathological features and postoperative prognosis in patients with prostate cancer (PC). Methods: A total of 112 cases of PC patients who underwent radical prostatectomy at CR WISCOM General Hospital affiliated to Wuhan University of Science and Technology from March 2020 to September 2021 were selected as study subjects; clinicopathological features such as age, clinical stage, lymph node metastasis, tumour diameter, Gleason score and so on were recorded; samples of cancer tissues and their paracancerous normal tissues of the PC patients were collected after the operation, and immunohistochemistry was used to detect SYNM, GALNT7, FOXF2 expression in the tissues; the relationship between SYNM, GALNT7, FOXF2 expression and clinicopathological characteristics of PC patients was analysed; three years of postoperative follow-up was conducted to record the survival of PC patients, and the factors affecting the postoperative prognosis of PC patients were analysed by unifactorial and multifactorial Cox regression; the SYNM was analysed by Kaplan-Meier curve, GALNT7, FOXF2 expression in relation to 3-year survival of PC patients. Results: SYNM was mainly expressed in the cytoplasm, GALNT7 was expressed in both the cell membrane and cytoplasm, and FOXF2 was mainly localised in the nucleus. The negative rate of SYNM in the observation group was 82.14% (92/112), which was significantly higher than that of the control group [11.61% (13/112)], χ2=111.883, P＜0.001); the positive rate of GALNT7 in the observation group was 85.71% (96/112), which was significantly higher than that of 4.46% (5/112) in the control group (χ2=149.315, P<0.001); the negative rate of FOXF2 in the observation group was 80.36% (90/112), which was significantly higher than that of 16.07% (18/112) in the control group (χ2=92.690, P<0.001). Among the PC patients, those with late clinical stage, lymph node metastasis, large tumour diameter, high Gleason score and high preoperative PSA level had significantly higher SYNM-negative rate, GALNT7-positive rate, and FOXF2-negative rate than those with early clinical stage, no lymph node metastasis, small tumour diameter, low Gleason score, and low preoperative PSA level (P<0.05). The prognostic death of PC patients was not related to age and clinical stage (P<0.05), but was related to tumour diameter, preoperative PSA level, Gleason score, and lymph node metastasis (P<0.05); preoperative PSA level, Gleason score, negative expression of SYNM, GALNT7-positive expression, and FOXF2-negative expression were all significant influencing factors for death within 3 years in PC patients (P<0.05); the 3-year survival rate was significantly higher in patients with SYNM-positive expression than in patients with negative expression (χ2= 24.633, P<0.05). The 3-year survival rate in patients with GALNT7-negative expression was higher than in patients with positive expression (χ2= 16.453, P<0.05). The 3-year survival rate of patients with positive expression of FOXF2 was significantly higher than that of patients with negative expression (χ2=24.977, P<0.05). Conclusion: The expression of SYNM, GALNT7, and FOXF2 in the tissues of PC patients is closely related to clinical stage, lymph node metastasis, tumor diameter, Gleason score, and preoperative PSA level. Negative expression of SYNM and FOXF2, and positive expression of GALNT7 are important factors affecting the postoperative prognosis of PC patients.
2025 Vol. 31 (8): 1302-1309 [Abstract] ( 10 ) HTML (1 KB)  PDF (1742 KB)  ( 3 )
1309 Relationship between High-Dose and Low-Dose Irradiation Volume of Different Parts of the Pelvis and Systemic Hematologic Toxicities in Patients Undergoing Intensity Modulated Radiotherapy for Cervical Cancer
Aynur Seyiti, HOU Youxiang, GAO Jie, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.013
Objective: To analyze the relationship between high-dose and low-dose irradiation volume of different parts of the pelvis and systemic hematologic toxicities in patients undergoing intensity modulated radiotherapy (IMRT) for cervical cancer. Methods: From April 2021 to April 2024, 184 patients with cervical cancer who underwent IMRT in the hospital were selected as the research subjects. All patients underwent hematologic toxicity tests. The relationship between high-dose and low-dose irradiation volume of different parts of the pelvis and systemic hematologic toxicities was analyzed. Results: RTOG grading results showed 58 cases of grade 1~2 (negative group) and 126 cases (68.48%) of grade 3~4 (positive group). There was no statistically significant difference in age, FIGO stage, type of cancer, V5, V30, and V40 Gy between the negative group and the positive group (P>0.05). There were statistically significant differences in chemotherapy cycle, concurrent radiochemotherapy, V10, and V20 Gy between the negative group and the positive group (P<0.05). Binary logistic regression analysis results showed that concurrent radiochemotherapy, V10, and V20 Gy were independent factors influencing the evaluation results of hematologic toxicities (P<0.05). Spearman analysis results showed that there was significant correlation between V10 and V20 Gy of the iliac bone and the grade of hematological toxicity. V5, V30 and V40 Gy of the iliac bone, V5, V10, V20, V30 and V40 Gy of the pelvic floor and lumbosacral spine were not significantly correlated with hematologic toxicities (P>0.05). Conclusion: Patients undergoing IMRT for cervical cancer have a high risk of systemic hematologic toxicities. Concurrent radiochemotherapy, V10 and V20 Gy are independent factors influencing the evaluation results of hematologic toxicities in patients undergoing IMRT for cervical cancer. Moreover, V10 and V20 Gy of the iliac bone are significantly correlated with hematologic toxicities.
2025 Vol. 31 (8): 1309-1314 [Abstract] ( 10 ) HTML (1 KB)  PDF (1175 KB)  ( 4 )
1315 Analysis of Influencing Factors of Prognosis in Patients with Acute Myocardial Infarction Complicated with Malignant Ventricular Arrhythmia and Construction of Nomogram Model
YANG Sa, WEN Fangfang, ZHANG Wen, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.014
Objective: To analyze the influencing factors of prognosis in patients with acute myocardial infarction (AMI) complicated with malignant ventricular arrhythmia (MVA), and to construct a nomogram model and verify it. Methods: A total of 286 patients with AMI complicated with MVA in our hospital from June 2020 to July 2024 were chosen and split into modeling group and verification group. The patients were followed up for the recovery within 72 hours after admission and divided into poor prognosis group and good prognosis group. The nomogram model was developed using single-factor and binary logistic regression analysis, and its prediction accuracy was evaluated using the ROC, calibration, and DCA curves. Results: Among the 286 patients, 58 patients had a poor prognosis (20.28%). Compared with the good prognosis group, the levels of systolic blood pressure (SBP), diastolic blood pressure (DBP) and left ventricular ejection fraction (LVEF) in the poor prognosis group were decreased, the levels of serum magnesium, urea, galectin-3 (Gal-3), growth differentiation factor-15 (GDF-15) and Creatine kinase isoenzyme (CK-MB) were increased, and the proportion of QRS-T angle>90° was increased (all P<0.05). SBP, QRS-T angle, LVEF, serum magnesium, urea and CK-MB were independent influencing factors of poor prognosis in patients with AMI complicated with MVA. ROC curve analysis showed that the AUC of the nomogram model for predicting poor prognosis in the modeling group was 0.914 (95%CI: 0.877-0.952), and the AUC of the validation group was 0.949 (95%CI: 0.875-1.000). The calibration curve fits well with the ideal curve. The net income of each range of the threshold probability value of the DCA curve analysis is greater than 0. Conclusion: The nomogram model constructed in this study has good efficacy and can provide reference for medical staff to evaluate the prognosis of patients with AMI complicated with MVA.
2025 Vol. 31 (8): 1315-1322 [Abstract] ( 9 ) HTML (1 KB)  PDF (1445 KB)  ( 3 )
1322 Effect of Hyperuricemia on Microvascular Disease in Elderly Patients with Type 2 Diabetes Mellitus
DIAO Xinchen, WANG Sihong, SONG Quanchao
DOI: 10.3969/j.issn.1006-6233.2025.08.015
Objective: To explore the effect of hyperuricemia (HUA) on microvascular disease (MVD) in elderly patients with type 2 diabetes mellitus (T2DM). Methods: 102 patients with T2DM admitted to the hospital from January 2021 to January 2024 were selected. The detection rate of HUA was statistically analyzed. The patients were divided into MVD group and non-MVD group according to the presence/absence of MVD. The incidence of HUA, general data, and biochemical indicators were compared between the two groups. Multivariate logistic regression analysis was used to screen risk factors for MVD in elderly patients with T2DM. Results: The detection rate of HUA in this study was 73.53%. There were 72 patients with MVD and 30 patients without. Univariate analysis showed that the proportions of patients with HUA and hypertension were significantly higher in the MVD group than in the non-MVD group. The inter-group differences in body mass index (BMI), course of disease and triglyceride were statistically significant (P<0.05). Multivariate logistic regression analysis showed that HUA, BMI, hypertension, and triglyceride were risk factors for MVD in elderly patients with T2DM (P<0.05). Conclusion: HUA is also one of the risk factors for MVD in elderly patients with T2DM. In addition, the relationship between BMI, hypertension, triglyceride and MVD in elderly patients with T2DM deserves clinical attention.
2025 Vol. 31 (8): 1322-1326 [Abstract] ( 6 ) HTML (1 KB)  PDF (1159 KB)  ( 2 )
1326 Relationship between Serum Leptin Free Fatty Acid and Adiponectin Levels and Insulin Resistance in Tibetan Patients with T2DM at Different Altitudes in Qinghai
LI Yuying, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.016
Objective: To investigate the relationship between levels of serum leptin, free fatty acid (FFA) and adiponectin and insulin resistance in Tibetan patients with type 2 diabetes mellitus (T2DM) at different altitudes in Qinghai. Methods: The medical records of 80 Tibetan patients with T2DM in the hospital were retrospectively analyzed from October 2022 to July 2024. According to the different altitudes of the patients, 41 cases were included in high altitude group ( ≥3000 meters), and 39 cases were enrolled as low altitude group (<3000 meters). Serum leptin, FFA, adiponectin and insulin resistance index (HOMA-IR) were detected. The differences in the above indicators were analyzed in the two groups. The correlation between serum leptin was explored by using Pearson correlation analysis., FFA and adiponectin and insulin resistance and to compare the correlation strength between indicators and HOMA-IR in the two groups. Results: The levels of serum leptin and serum FFA and HOMA-IR in high altitude group were higher than those in low altitude group (P<0.05), while the adiponectin level was lower than that in low altitude group (P<0.05). Serum leptin and FFA levels were positively correlated with HOMA-IR in both groups (P<0.05), and adiponectin level was negatively correlated with HOMA-IR in both groups (P<0.05). From the distribution of r value, the correlation effect in high altitude areas was stronger, nevertheless, there was no statistically significant difference between the groups (P>0.05). Conclusion: In Tibetan patients with T2DM in high altitude areas, serum leptin level and FFA level show high trends, while adiponectin level is relatively low, and the above indicators are closely related to insulin resistance in both high altitude and low altitude areas, no difference in the strength of association has been found between different altitude regions.
2025 Vol. 31 (8): 1326-1330 [Abstract] ( 8 ) HTML (1 KB)  PDF (1164 KB)  ( 3 )
1330 Correlation of UACR with Body Mass Index and Course of Diabetes Mellitus in Elderly Diabetic Patients and Risk Factors of Abnormal UACR
TIAN Min, SHEN Qiongna, SANG Yuankang
DOI: 10.3969/j.issn.1006-6233.2025.08.017
Objective: To analyze the correlation between urinary albumin/creatinine ratio (UACR) and body mass index (BMI) and course of diabetes mellitus (DM) in elderly patients with DM, and analyze the risk factors affecting abnormal UACR. Methods: Totally 117 elderly patients with DM in the hospital from June 2024 to October 2024 were collected as the study subjects. The UACR of the subjects was measured, and the BMI and DM course were compared among patients with different levels of UACR. The correlation between UACR and BMI and DM course in elderly diabetic patients was explored by Pearson correlation analysis. The patients were divided into normal group (UACR< 30mg/g) and abnormal group (UACR≥30mg/g) based on the UACR level. Logistic regression analysis was utilized to analyze the risk factors affecting the abnormal UACR. Results: There were no obvious differences in age, gender, glycosylated hemoglobin, high density lipoprotein cholesterol, total cholesterol, and low density lipoprotein cholesterol between groups (P>0.05). The BMI, DM course, proportion of concurrent hypertension and triacylglycerol in the abnormal group were higher than those in the normal group (P<0.05). Logistic regression analysis found that BMI, DM course, concurrent hypertension, and triacylglycerol were independent risk factors for abnormal UACR (OR: 1.134, CI: 1.018~1.263; OR: 1.772, CI: 1.231~2.551; OR: 1.627, CI: 1.153~2.298; OR: 1.314, CI: 1.072~1.611, P<0.05). Conclusion: UACR in elderly patients with DM is closely related to BMI and course of DM. BMI, DM course, concurrent hypertension and triacylglycerol are independent risk factors for abnormal UACR. It is helpful for early detection of renal damage in elderly diabetic patients and guidance of the formulation of personalized treatment regimen.
2025 Vol. 31 (8): 1330-1333 [Abstract] ( 11 ) HTML (1 KB)  PDF (1156 KB)  ( 4 )
1334 Effects of Platelet-Rich Fibrin on Guided Bone Regeneration during Maxillary Anterior Tooth Implantation and the Impact on Dental Function
LI Sensen, ZHU Bin, HUANG Jing, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.018
Objective: To observe the effects of platelet-rich fibrin (PRF) on guided bone regeneration during maxillary anterior tooth implantation and the impact on dental function. Methods: A total of 102 patients who underwent maxillary anterior tooth implantation in the hospital between January 2021 and December 2023 were selected and randomly assigned to the traditional group (51 cases) and the experimental group (51 cases) by drawing lots. For the traditional group, absorbable collagen membrane combined with Bio-Oss bone powder was used for guided bone regeneration during surgery. For the experimental group, PRF was used for guided bone regeneration during surgery. The two groups were compared on postoperative pain, success rate of implantation, mucosa healing on day 7 after surgery, bone regeneration effect 6 months after surgery, dental function, and aesthetic effect. Results: The success rates of implantation in the two groups were close (P>0.05). Postoperative pain grading results in the experimental group were better than those in the traditional group (P<0.05). The experimental group obtained better mucosa healing and bone regeneration as compared with the traditional group (P<0.05). The scores for dental function in various dimensions of the experimental group were higher as compared with the traditional group (P<0.05). The pink aesthetic score (PES) and white aesthetic score (WES) of the experimental group were better than those of the traditional group (P<0.05). Conclusion: Applying PRF guided bone regeneration during maxillary anterior tooth implantation can promote mucosa healing and bone regeneration while alleviating postoperative pain and improving aesthetic effects and dental function.
2025 Vol. 31 (8): 1334-1338 [Abstract] ( 10 ) HTML (1 KB)  PDF (1156 KB)  ( 3 )
1338 Analysis of the Efficacy of Percutaneous Nephrostomy Drainage at Different Timing in the Treatment of Upper Urinary Tract Calculi Complicated with Infection
YU Jianhong, ZHANG Wenjuan, WANG Qianlong, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.019
Objective: To analyze the efficacy of percutaneous nephrostomy (PCN) drainage in the treatment of upper urinary tract stones with infection at different timeing. Methods: Totally 102 patients with upper urinary tract calculi and infection in the hospital from October 2019 to October 2024 were divided into 2h group (n=42, PCN drainage within 2h after admission), 12h group (n=34, PCN drainage within 2~12h after admission) and 24h group (n=26, PCN drainage within 12~24h after admission) according to the different timing of PCN drainage. The surgical time, intraoperative blood loss and one-time puncture success rate, and changes of body temperature and infection-related indicators [white blood cell count (WBC), C-reactive protein (CRP), procalcitonin (PCT)] before surgery and at 3 days after operation were compared among the three groups. The infection control time, hospitalization time and complications in the three groups were recorded. Results: There were no statistical differences in surgical time, intraoperative blood loss and success rate of one-time puncture among the three groups (P>0.05). The hospitalization time in the 2h and 12h groups was shorter than that in the 24h group (P<0.05), but no statistical differences were observed in infection control time and hospitalization time between the 2h group and the 12 h group (P>0.05). At 3 days after surgery, the body temperature, WBC, CRP and PCT in the three groups were lower than those before surgery (P<0.05), and the above indicators in the 24h group were higher than those in the 2h group and the 12h group (P<0.05), but the above indicators were not statistically different between the 2h group and the 12h group (P>0.05). The complications revealed no statistical differences among the three groups (P>0.05). Conclusion: PCN drainage within 12h after admission is effective in the treatment of patients with upper urinary tract calculi and infection, and it can effectively control infection and shorten hospitalization time.
2025 Vol. 31 (8): 1338-1343 [Abstract] ( 11 ) HTML (1 KB)  PDF (1183 KB)  ( 4 )
1343 Relationship Between Color Doppler Ultrasonic Blood Flow Parameters and the Expression of PI3K and Akt in Her2-Positive Breast Cancer Tissues and Clinical Prognosis
LI Xiaoxin, JIN Xin, WANG Pei, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.020
Objective: To explore the relationship of color Doppler ultrasound blood flow parameters with expressions of tissue phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt) and clinical prognosis in human epidermal growth factor receptor 2 (Her2)-positive breast cancer, and to evaluate the evaluation value on prognosis. Methods: Totally 103 patients with Her2-positive breast cancer who received treatment in the hospital from January 2020 to January 2023 were enrolled as study subjects. According to the prognosis status, the enrolled patients were divided into poor prognosis group (n=27) and good prognosis group (n=76). All subjects received color Doppler ultrasound examination to obtain blood flow parameters [resistance index (RI), pulsatility index (PI), peak flow rate (Vmax)], and the expressions of PI3K and Akt in cancer tissues were determined. The correlation between PI, RI, Vmax and Her2-positive breast cancer tissue PI3K and Akt expressions or prognosis was explored by Spearman correlation analysis. Receiver operating characteristic (ROC) curve was adopted to analyze the evaluation value of PI, RI and Vmax on prognosis. Results: The RI, PI and Vmax and positive expression rates of PI3K and Akt in the poor prognosis group were higher than those in the good prognosis group (P<0.05). Correlation analysis showed that PI, RI and Vmax were positively correlated with Her2-positive breast cancer tissue PI3K expression and Akt expression and poor prognosis (rRI=0.284, 0.206, 0.443; rPI=0.213, 0.244, 0.464; rVmax=0.245, 0.221, 0.355, P<0.05). ROC curve suggested that the area under the curve (AUC), sensitivity and specificity of combination of RI, PI and Vmax in evaluating the prognosis of patients with Her2-positive breast cancer were 0.919, 88.89% and 89.47%. The combined evaluation efficiency was better than that of each index alone (P<0.05). Conclusion: Blood flow parameters such as PI, RI and Vmax are positively correlated with PI3K expression, Akt expression and poor prognosis in patients with Her2-positive breast cancer. Analyzing the above blood flow parameters can provide some reference value on the prognosis evaluation of patients, and guide the formulation of individualized treatment regimen.
2025 Vol. 31 (8): 1343-1348 [Abstract] ( 11 ) HTML (1 KB)  PDF (1339 KB)  ( 3 )
1348 Clinical Study on CT-Guided Posterior Lesion Debridement and Bone Graft Fusion with Internal Fixation Combined with Thoracolumbar Decompression in the Treatment of Spinal Tuberculosis
XIE Liang, GE Yulong, ZHANG Yanxiang, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.021
Objective: To investigate the clinical efficacy of CT-guided posterior debridement, bone graft fusion, internal fixation combined with thoracolumbar decompression in the treatment of spinal tuberculosis. Methods: A total of 120 patients with spinal tuberculosis admitted to the hospital between June 2020 and June 2023 were selected for retrospective analysis and divided into anterior decompression group (anterior lesion debridement and bone graft fusion with internal fixation combined with thoracolumbar decompression surgery) and posterior decompression group (posterior lesion debridement and bone graft fusion with internal fixation combined with thoracolumbar decompression surgery) according to different surgical procedures. The propensity matching method was used to exclude confounding factors such as age and disease course. Finally, 58 cases in anterior decompression group and 62 cases in posterior decompression group were obtained. The perioperative indicators [surgical time, intraoperative blood loss, postoperative drainage volume], kyphosis Cobb angle immediately after surgery and at 6 months after surgery, spinal function [Kirkaldy-Willis functional classification] and spinal cord injury [American Spinal Injury Association Spinal Cord Injury (ASIA)] before surgery and at 6 months after surgery and incidence rates of surgical complications [dural tear, nerve injury, chronic back pain] within 6 months after surgery were compared between the two groups of patient. Results: The surgical time, intraoperative blood loss and postoperative drainage volume in posterior decompression group were significantly shorter or less than those in anterior decompression group (P<0.05). There was no significant difference in inter-group effect and interaction between groups before operation, immediately after operation and 6 months after operation (P>0.05), and the time effect was statistically significant (P<0.05). The Cobb angle of kyphosis in the two groups immediately after operation and 6 months after operation was less than that before operation (P<0.05), and there was no difference between the two groups (P>0.05). At 6 months after surgery, the Kirkaldy-Willis function score was significantly higher in both groups than that before surgery (P<0.05) while the ASIA rating was significantly lower than that before surgery (P<0.05), but there were no obvious differences between groups (P>0.05). The incidence rates of complications such as dural tear, nerve injury and chronic back pain were slightly lower in posterior decompression group compared with those in anterior decompression group within 6 months after surgery (P>0.05). Conclusion: CT-guided posterior lesion debridement and bone graft fusion with internal fixation combined with thoracolumbar decompression can significantly reduce the surgical time and blood loss, and promote the recovery of spinal structure and function in the treatment of spinal tuberculosis.
2025 Vol. 31 (8): 1348-1354 [Abstract] ( 10 ) HTML (1 KB)  PDF (1196 KB)  ( 3 )
1354 Influences of Autologous Limbal Stem Cell Transplantation Combined with Amniotic Membrane Transplantation on Patients with Pterygium Excision
HU Na, LI Ting
DOI: 10.3969/j.issn.1006-6233.2025.08.022
Objective: To investigate the influences of autologous limbal stem cell transplantation (LSCT) combined with amniotic membrane transplantation (AMT) on visual acuity recovery status and tear function in patients undergoing pterygium excision. Methods: A total of 102 patients who received pterygium excision in the hospital from January 2023 to October 2024 were selected as research subjects. Patients were divided into a control group and a combined group according to the simple randommethod using a random number table, with 51 cases in each group. The control group was treated with pterygium excision combined with LSCT, while the combined group was treated with AMT on the basis of the control group. The postoperative swelling time, congestion regression time, corneal epithelial healing time and postoperative recurrence rate and visual acuity recovery status [uncorrected visual acuity (UCVA), corneal astigmatism (CA)], tear secretion function [Schirmer test (SIT)], ocular surface conditions [noninvasive Keratograph tear breakup time (NIKBUT), tear meniscus height (MIKTMH)] and tear inflammatory factors [interleukin (IL)-1β, transforming growth factor (TGF)-β1] before surgery and at 2 weeks after surgery were compared between the groups. The incidence rates of postoperative complications and discomfort were recorded. Results: There were no statistical differences in UCVA, CA, SIT, NIKBUT, MIKTMH, IL-1β and TGF-β1 between the two groups before surgery (all P>0.05). The postoperative swelling time, congestion regression time and corneal epithelial healing time in combined group were significantly shorter than those in control group, and the postoperative recurrence rate was significantly lower than that in control group (all P<0.05). After surgery, the UCVA, SIT, NIKBUT and MIKTMH in the two groups were higher than those before surgery, and the absolute differences of UCVA, SIT, NIKBUT and MIKTMH before and after surgery in combined group were greater than those in control group (all P<0.05). The CA in both groups of patients was lower than that before surgery, and the absolute difference of CA before and after surgery was greater in combined group than that in control group (all P<0.05). After surgery, the level of IL-1β in tear in the two groups was reduced compared with that before surgery, and the absolute difference of IL-1β before and after surgery in combined group was lower than that in control group, and the level of TGF-β1 was enhanced compared to before surgery, and the absolute difference of TGF-β1 before and after surgery was higher in combined group than that in control group (all P<0.05). There was no statistical significance in the total incidence rate of postoperative complications between groups (P>0.05). The incidence rate of postoperative discomfort in combined group was lower compared to control group (P<0.05). Conclusion: LSCT combined with AMT is beneficial to the recovery of postoperative visual acuity, improvement of tear function and reduction of postoperative discomfort in patients with pterygium excision.
2025 Vol. 31 (8): 1354-1359 [Abstract] ( 10 ) HTML (1 KB)  PDF (1177 KB)  ( 4 )
1360 Efficacy and Safety Comparison of two Surgical Approaches for Varicocele
FAN Bo, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.023
Objective:To compare the efficacy and complications of retroperitoneal and inguinal approaches for varicocele surgery. Methods: This study included 94 patients undergoing varicocelectomy (49 retroperitoneal vs. 45 inguinal approaches) between 2019 and 2023, with comparisons made for intraoperative outcomes, complications, and recurrence rates. Results: Both surgical approaches achieved technical success. The inguinal approach group demonstrated significantly longer operative time than the retroperitoneal group (P<0.05). However, the inguinal approach was associated with shorter postoperative hospitalization (P<0.05), lower pain scores (P<0.05), versus the retroperitoneal approach. There's no significant difference in blood loss complication rates between groups (P>0.05). The retroperitoneal approach showed superior outcomes in terms of lower recurrence rates (P<0.05). Conclusion: The retroperitoneal approach for varicocelectomy demonstrated superior outcomes in operative duration and recurrence rates compared to the inguinal approach. Conversely, the inguinal approach was associated with shorter postoperative hospitalization and lower pain scores. Both surgical techniques proved effective for varicocele treatment. In clinical practice, the surgical approach should be individualized based on patient characteristics.
2025 Vol. 31 (8): 1360-1363 [Abstract] ( 10 ) HTML (1 KB)  PDF (1146 KB)  ( 5 )
1364 Correlation between Fibrinogen to Albumin Ratio, Platelet Parameters and Coronary Artery Lesion Degree
MENG Xin, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.024
Objective: To explore the correlation of fibrinogen to albumin ratio (FAR) and platelet parameters with degree of coronary artery lesion. Methods: The clinical data of 154 patients who received coronary angiography in the hospital were retrospectively analyzed from January 2021 to August 2024. The enrolled patients were assigned into coronary heart disease (CHD) group (n=108) and reference group (n=46) by coronary angiography results. By means of the evaluation results of Gensini score system, the patients in the CHD group were classified into mild-to-moderate lesion group (80 cases, Gensini score<60 points) and severe lesion group (28 cases, Gensini score≥60 points). The FAR and platelet parameters were compared among the reference group, mild-to-moderate lesion group and severe lesion group. The correlation of FAR and various platelet parameters with coronary artery lesion degree was explored by Spearman correlation coefficient. The area under the receiver operating characteristic (ROC) curve (AUC) was used to test the value of combination of FAR and platelet parameters in assessing the degree of coronary artery lesion. Results: The FAR, MPV and PDW in the mild-to-moderate lesion group and the severe lesion group were higher than those in the reference group, and the above indicators in the severe lesion group were higher compared with those in the mild-to-moderate lesion group (P<0.05). PLT was lower in the mild-to-moderate lesion group and the severe lesion group than that in the reference group, and was also lower in the severe lesion group compared to the mild-to-moderate lesion group (P<0.05). Spearman correlation coefficient analysis revealed that FAR, MPV and PDW were positively correlated with coronary artery lesion degree (r=0.473, 0.451, 0.468, P<0.05), and PLT was negatively associated with the degree of coronary artery lesion (r=-0.397, P<0.05). According to the AUC predictive value under the ROC curve, the AUC of FAR combined with platelet parameters was 0.938 (95%CI: 0.881~0.994) in assessing the degree of coronary artery lesion (P<0.05). Conclusion: FAR and platelet parameters are significantly correlated with the degree of coronary artery lesion, and the combined detection of various indicators is effective in predicting coronary artery lesion in CHD. These indicators can be comprehensively analyzed as the preferred biomarkers for clinical diagnosis of coronary artery disease and coronary artery lesion degree.
2025 Vol. 31 (8): 1364-1369 [Abstract] ( 14 ) HTML (1 KB)  PDF (1312 KB)  ( 4 )
1369 Correlation between Thickness of the Inferior Capsule, Serum Il-1 β Cox-2 and the Severity of Adhesive Shoulder Arthritis and Its Diagnostic Value
LIANG Tingting, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.025
Objective: To investigate the thickness of the inferior capsule (IC) of the glenohumeral joint in adhesive shoulder arthritis, the levels of serum interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2), and analyze their correlation with disease severity and diagnostic value for adhesive shoulder arthritis. Methods: A total of 210 patients with adhesive shoulder arthritis from July 2022 to July 2024 were selected as the study group, and 105 healthy volunteers during the same period were selected as the control group. The IC thickness, serum IL-1β, and COX-2 levels were compared between the two groups. The Constant-Murley Shoulder Function (CMS) score and Visual Analogue Scale (VAS) score were used as the basis for evaluating the severity of the disease, and were divided into four subgroups. IC thickness, serum IL-1β, and COX-2 levels were compared. The diagnostic value of IC thickness, serum IL-1β, and COX-2 levels was analyzed for adhesive shoulder arthritis. Correlation analysis was used to analyze the correlation between IC thickness, serum IL-1β, COX-2 levels, and CMS scores and VAS scores. Results: The thickness of the IC on the affected side of the study group was greater than that on the left side of the control group (3.35±0.94mm vs 1.26±0.27mm, t=22.312, P<0.001), and on the right side of the control group (3.35±0.94mm vs 1.20±0.24mm, t=23.048, P<0.001); the thickness difference between the two sides of the IC in the research group was greater than that in the control group (2.14±0.63 mm vs 0.06±0.02 mm, t=33.796, P<0.001); the levels of serum IL-1β and COX-2 in the study group were (28.15±9.85) ng/mL and (23.69±7.28) pg/mL, respectively, which were higher than those in the control group at (13.64±4.17) ng/mL and (12.41±4.05) pg/mL (P<0.05). The thickness of the affected side of the IC, serum levels of IL-1β, and COX-2 showed a gradual downward trend in C1, C2, C3, and C4 subgroups, while the thickness of the affected side of the IC, serum levels of IL-1β, and COX-2 showed a gradual upward trend in V1, V2, V3, and V4 subgroups (P<0.05). The AUC of IC thickness, serum IL-1β, COX-2 levels, and their combined diagnosis for adhesive shoulder arthritis on the affected side were 0.776, 0.770, 0.753, and 0.923, with sensitivities of 80.48%, 69.52%, 64.29%, and 80.00%, and specificities of 61.90%, 74.29%, 74.29%, and 91.43%. The combined diagnostic value was significantly higher than that of each individual indicator (Z=4.896, 4.871, 5.439, all P<0.001). The contingency correlation analysis showed that the thickness of the affected side of the IC, serum IL-1β, and COX-2 levels were negatively correlated with CMS scores and positively correlated with VAS scores (P<0.05). Conclusion: The thickness of IC in patients with adhesive shoulder arthritis increases, and the levels of serum IL-1β and COX-2 increase, which are closely related to the severity of the disease. Combined detection of their levels has certain diagnostic value for adhesive shoulder arthritis, and combined detection can improve diagnostic efficiency.
2025 Vol. 31 (8): 1369-1375 [Abstract] ( 10 ) HTML (1 KB)  PDF (1686 KB)  ( 4 )
1376 Study on the Value of Quantitative Parameters of Dynamic Enhanced Magnetic Resonance Imaging Combined with Diffusion Weighted Magnetic Resonance Imaging in the Diagnosis of Myelopathy
HAN Dong, SUN Yuan, LIU Chunjie, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.026
Objective:To analyze the value of quantitative parameters of dynamic enhanced magnetic resonance imaging (DCE-MRI) combined with diffusion-weighted imaging (DWI) in the diagnosis of myelopathy. Methods: A total of 75 patients with myelopathy admitted to our hospital from September 2020 to March 2022 were selected for routine MRI scan +DWI examination. DTI and DKI were added to the corresponding locations where abnormal signals were found, and DCE enhanced scanning was followingly performed. The pathology of spinal tumors was regarded as the gold standard. The DCE-M RI and DWI quantitative parameter indexes [DTI (FA, ADC value), DKI (MK value), IVIM-DWI (D, D* and f value), DCE (Ktrans, Kep, Ve value)] and microvascular density (MVD) of all groups were compared. The diagnostic value of DCE-MRI combined with DWI in myelopathy was analyzed. Results: Among the 75 patients with spinal cord lesions who were pathologically examined, 15 cases (20.00%) of spinal cord tumors and 60 cases (80.00%) of non-spinal cord tumors were confirmed, including 20 cases (26.67%) of cervical medullary degeneration due to disc herniation compression, 20 cases of MS 26.67%), and 20 cases (26.67%) of optic myelitis disease spectrum. Ktrans was higher in the spinal cord tumor group compared with the non-spinal cord tumor group, and ADC, FA value, and D levels were lower compared with the non-spinal cord tumor group (P<0.05). The MVD of spinal cord tumor group was (24.61±4.41) /mm2, which was higher than that of non-spinal cord tumor group (19.57±4.06) /mm2 (P<0.05). Pearson correlation analysis showed that MVD was negatively correlated with ADC, FA and D (r<0, P<0.05), and positively correlated with Ktrans (r>0, P>0.05);the results of plotting ROC curves showed that ADC, FA value, D, Ktrans, and combined detection all had AUC>0.70 for spinal cord tumors, which had predictive value, with the combined detection having the highest value; the diagnosis correct rate of DCE-MRI combined with DWI was 93.33% (70/75), and the agreement with pathological examination was k=0.911. Conclusion: DCE-MRI and DWI quantitative parameters can effectively diagnose and differentiate different types of spinal cord diseases, and are closely related to MVD, which is helpful to provide reference for the early identification and prevention of spinal cord diseases.
2025 Vol. 31 (8): 1376-1381 [Abstract] ( 10 ) HTML (1 KB)  PDF (3189 KB)  ( 3 )
1382 Value of ABCD2 Score Combined with Serum Copeptin and Lp-PLA2 Levels on Predicting the Onset of Ischemic Stroke within 7 days of Transient Ischemic Attack
DU Yun, FAN Qingyu, Bu Ning, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.027
Objective: To explore the application value of ABCD2 score combined with serum copeptin and lipoprotein-associated phospholipase (Lp-PL) A2 levels in predicting the prognosis of patients with transient ischemic attack (TIA) within 7 days. Methods: The clinical data of 114 patients with TIA were retrospectively analyzed from January 2020 to December 2023. According to the occurrence of ischemic stroke (CIS) after TIA, the patients were divided into CIS occurrence group (n=18) and CIS non-occurrence group (n=96). The general data of the two groups were collected. The ABCD2 scoring system was used to evaluate all patients, and the levels of serum copeptin and Lp-PLA2 were detected. Multivariate Logistic regression model was used to analyze the independent risk factors affecting the prognosis of patients with TIA. Receiver operating characteristic (ROC) curve and the maximum Youden index were adopted to analyze the predictive value of each factor on the prognosis of patients with TIA. Results: The ABCD2 score, serum copeptin and Lp-PLA2 levels in the CIS occurrence group were higher than those in the CIS non-occurrence group (P<0.05). Logistic regression analysis showed that ABCD2 score, copeptin and Lp-PLA2 were independent risk factors for TIA. ROC curve results showed that the area under the curve (AUC) of ABCD2 score, serum copeptin and Lp-PLA2 levels were 0.779 (0.702~0.855), 0.843 (0.780-0.906) and 0.760 (0.681~0.838), respectively. The cut-off values of each index were 4 points, 174.34mg/L and 16.49pmol/L, respectively. The area under the curve (AUC) of combined prediction was 0.925 (0.881~0.968), and the prediction efficiency was the highest. Conclusion: Clinically, it is necessary to take early warning measures for TIA patients with ABCD2 score>4point, serum copeptin level>16.49pmol/L and serum Lp-PLA2>174.34mg/L to prevent poor prognosis.
2025 Vol. 31 (8): 1382-1386 [Abstract] ( 12 ) HTML (1 KB)  PDF (1222 KB)  ( 3 )
1386 Influences of Different Surgical Methods on Clinical Efficacy Postoperative Pain and Safety in Patients with Total Hysterectomy
WANG Xiangqiong, JIANG Yezhong, WANG Weiping, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.028
Objective: To investigate the clinical effects of different surgical methods on patients undergoing total hysterectomy. Methods: Relevant clinical data of patients undergoing total hysterectomy in the hospital were retrospectively analyzed between January 2021 and December 2023, and a total of 113 patients were included as study subjects according to the exclusion criteria and divided into total abdominal hysterectomy (TAH) group (46 cases), total vaginal hysterectomy (TVH) group (48 cases) and laparoscopic assisted vaginal hysterectomy (LAVH) group (19 cases). The baseline data, clinical indicators, postoperative Visual Analogue Scale (VAS) score and postoperative complications were compared among TAH group, TVH group and LAVH group. Results: The surgical time in the LAVH group was longer than that in the TAH group and the TVH group, and the ambulation time, hospitalization time and anal exhaust time in the LAVH group after surgery were shorter than those in the TAH group and the TVH group, and the above indicators were shorter in the TVH group than those in the TAH group (P<0.05). The sedative use rate was 8.33% in the TVH group (4 cases) and was 10.53% in the LAVH group (2 cases), which was lower than 78.26% in the TAH group (29 cases) (P<0.05). The VAS scores in the LAVH group at 24 and 48 hours after surgery were lower than those in the TAH group and the TVH group, and the above scores in the TVH group were lower compared with those in the TAH group (P<0.001). The incidence of postoperative complications after surgery was 4.16% in the TVH group (2 cases) and 5.26% in the LAVH group (1 case), which was lower than 19.56% in the TAH group (9 cases) (P<0.05). Conclusion: Both TVH and LAVH have good safety. Compared with TAH and TVH, LAVH is more minimally invasive, which can accelerate the recovery process of patients and enable patients to be discharged as soon as possible.
2025 Vol. 31 (8): 1386-1391 [Abstract] ( 12 ) HTML (1 KB)  PDF (1193 KB)  ( 463 )
1392 Influences of Total Intravenous Anesthesia with Remimazolam Besylate and Propofol on Hemodynamics and Recovery Quality in Patients with Thyroid Surgery
WANG Ying, et al
DOI: 10.3969/j.issn.1006-6233.2025.08.029
Objective: To investigate the influence of total intravenous anesthesia with remimazolam besylate and propofol on hemodynamics and recovery quality in patients undergoing thyroid surgery. Methods: A total of 138 patients undergoing thyroid surgery were classified into control group (n=69, propofol total intravenous anesthesia) and observation group (n=69, remimazolam besylate total intravenous anesthesia) by adopting the random number table method. The hemodynamic indicators at different time points and surgical conditions and anesthetic dosage, and sedation score (RSS) at 5min, 30 min and 60 min after extubation were compared between groups, and the postoperative adverse reactions were recorded. Results: The heart rate (HR) and mean arterial pressure (MAP) were statistically different before tracheal intubation (T1) and at 1 min after tracheal intubation (T2), the starting of surgery (T3), 30 min after the starting of surgery (T4) and the time of postoperative recovery (T5) (Ftime-point=1.142, 2.092, P<0.05). Compared with the control group, the observation group had higher MAP and HR (Fbetween group=33.701, 21.421, P<0.05), and the change trends of MAP and HR were different between groups (Finteraction=1.661, 1.030, P<0.05). The onset time and intubation time were shorter in the observation group (P<0.05). The remifentanil dosage and sedative dosage in the observation group were less (P<0.05). Compared with the control group, the recovery time of spontaneous breathing, eye opening time and orientation recovery time were shorter in the observation group (P<0.05). There was a statistical significance in RSS score at 5min and 30 min after recovery (Ftime-point=16.191, P<0.05). The observation group had lower RSS score than the control group (F between group= 38.042, P<0.05). There was a statistical difference in the change trend of RSS score between the observation group and the control group (Finteraction=7.230, P<0.05). The number of dreaming cases in the observation group during anesthesia was more than that in the control group (P<0.05), and no dysphoria and delirium occurred in both groups, and there was no obvious difference in incidence rate of nausea and vomiting between both groups (P>0.05). Conclusion: Remimazolam besylate general anesthesia has a better application effect during thyroid surgery compared to propofol total intravenous anesthesia, and it can better promote the spontaneous breathing after anesthesia, relieve the agitation, and maintain the hemodynamic stabil
2025 Vol. 31 (8): 1392-1396 [Abstract] ( 12 ) HTML (1 KB)  PDF (1164 KB)  ( 6 )
1396 Evaluation of the Efficacy of Different Doses of rt-PA Intravenous Thrombolysis Combined with Rosuvastatin in the Emergency Treatment of Ischemic Stroke
LI Chunxia, MI Jingjing
DOI: 10.3969/j.issn.1006-6233.2025.08.030
Objective: To analyze the efficacy of combined application of intravenous thrombolysis with different doses of recombinant tissue plasminogen activator (rt-PA) and rosuvastatin in the ischemic stroke emergency treatment. Methods: Two hundred and ten patients with ischemic stroke in the hospital were enrolled between July 2021 and July 2024, and were divided into low-dose group (n=108, 0.6mg/kg) and high-dose group (n=102, 0.9mg/kg) according to the dose of rt-PA. The efficacy in the two groups was evaluated. The coagulation indexes, neurological function and activities of daily living were compared between both groups before and after treatment, and the safety was assessed. Results: The total effective rate in the low-dose group with 90.74% was not statistically different from 83.33% in the high-dose group (P>0.05). At 7 days after treatment, the fibrinogen (Fib) level in the two groups was reduced while the D-dimer (D-D) level was enhanced (P<0.05). There was no statistical significance in the difference value of Fib level before and after treatment between the low-dose group and the high-dose group (P>0.05), but the difference value of D-D level before and after treatment in the low-dose group was higher in comparison with the high-dose group (P<0.05). A statistical difference was exhibited in the National Institutes of Health Stroke Scale (NIHSS) score at different time points (P<0.05), and the NIHSS score revealed no statistical difference between the two groups (P>0.05), and the interaction effect between time-point and group was not statistically significant (P>0.05). The barthel index was statistically different at different time points (P<0.05). The barthel index revealed no statistical difference between groups (P>0.05), and no statistical significance was found in the interaction effect between time-point and group (P>0.05). The incidence rates of complications were lower in the low-dose group compared to the high-dose group (P<0.05). Conclusion: The efficacy of low-dose rt-PA intravenous thrombolysis combined with rosuvastatin and its effects on coagulation function, neurological function and activities of daily living in the emergency treatment of ischemic stroke are comparable to those of high-dose group. However, compared with high-dose rt-PA, low-dose rt-PA intravenous thrombolysis has higher safety.
2025 Vol. 31 (8): 1396-1401 [Abstract] ( 10 ) HTML (1 KB)  PDF (1189 KB)  ( 8 )
Copyright © Editorial Board of HeBei Med
Supported by:Beijing Magtech