Home  |  About Us  |  Editorial Board  |  Instruction  |  Subscribe  |  Advertisement  |  Messages  |  Contact Us  |  中文

Office Online         Journal Just Accepted Current Issue Archive Advanced Search Most Read Most Download Email Alert            Download More...              Links More...

2025 Vol. 31, No. 11 Published: 2025-11-30 1761 Exploring the Effects of Salidroside on Endothelial Dysfunction in Rats with Hypertensive Disorder of Pregnancy Based on AMPK-eNOS Pathway ZHENG Tinghua, SONG Huizhen, WANG Lili, et al DOI: 10.3969/j.issn.1006-6233.2025.11.01 Objective: To explore the effects of salidroside (Sal) on endothelial dysfunction in hypertensive disorder of pregnancy (HDP) rats based on AMPK-eNOS pathway. Methods: Rat models of HDP were constructed. The successfully modeled rats were randomly classified into HDP group, Sal-L group, Sal-H group, and Sal-H+Compound C (AMPK inhibitor) group, with 12 rats in each group. Another 12 normal healthy rats were made the control group. Blood pressure and 24h urinary protein were measured before and after administration. ELISA was performed to measure serum endothelial function biomarkers and inflammatory factors. HE staining was performed to observe pathological changes in kidney tissue and placenta. Immunohistochemistry was used to detect sFLT-1 and PLGF. Western blot was used to measure the AMPK-eNOS pathway related proteins. Results: For the control group, the HDP group showed aggravated renal tissue pathological damage, increased glomerular edema, and even sclerosis, dilation of renal cysts, thickening of basement membrane, shrinkage of placental structure, reduction of placental villi, and even fibrin like necrosis, and there was an increase in vacuolated cells in the trophoblast layer and infiltration of inflammatory cells. The mean arterial pressure, 24h UTP, ET-1, TXB2, TNF-α, IL-6, IL-17 levels, and sFLT-1 expression increased, while the NO level and PLGF, p-AMPK/AMPK, p-AKT/AKT, and p-eNOS/eNOS expression decreased (P<0.05). The pathological damage of renal tissue and placental tissue in the Sal-L group and Sal-H group was alleviated compared with the HDP group, with a decrease of the mean arterial pressure, 24h UTP, ET-1, TXB2, TNF-α, IL-6, IL-17 levels, and sFLT-1, and an increase in the NO level and PLGF, p-AMPK/AMPK, p-AKT/AKT, and p-eNOS/eNOS expression (P<0.05). The Sal-H+Compound C group showed an increase in mean arterial pressure, 24h UTP, ET-1, TXB2, TNF-α, IL-6, IL-17 levels, and sFLT-1 expression, and a decrease in NO level and PLGF, p-AMPK/AMPK, p-AKT/AKT, and p-eNOS/eNOS expression than Sal-H group (P<0.05). Conclusion: Salidroside can improve endothelial dysfunction in HDP rats and is associated with the activation of AMPK-eNOS pathway. 2025 Vol. 31 (11): 1761-1766 [Abstract] ( 236 ) HTML (1 KB)  PDF (2472 KB)  ( 14 ) 1767 Effect of Sesamol on Skin Damage in Chronic Eczema Rats by Adjusting the MAPK/NF-κB Signaling Pathway WANG Li, ZHOU Xiaoli, SUN Zhenhui, et al DOI: 10.3969/j.issn.1006-6233.2025.11.02 Objective: To explore the effect of sesamol (SE) on skin damage in chronic eczema (CE) rats by adjusting the mitogen-activated protein kinase (MAPK)/nuclear factor-κB (NF-κB) signaling pathway. Methods: A CE model rat was established, and the successfully modeled rats were stochastically divided into a CE group, a SE-L group, a SE-H group (external application of 0.7% and 2.1% SE dissolved in 0.1% CMC-Na solution), and a SE-H+Anisomycin group (external application of 2.1% SE+1.4mg/mL MAPK activator Anisomycin dissolved in anhydrous ethanol), each with 12 rats. Another 12 normal rats were stochastically selected as NC group (shaved only, without any other treatment). The rats in NC and CE groups were treated with equal doses of 0.1% CMC-Na and anhydrous ethanol for 10 days. ELISA method was used to detect serum IgE, His, IL-1β, and TNF-α. HE staining and methylamine blue staining were used to observe pathological changes and the number of mast cells in skin lesions. Immunohistochemistry was used to detect the Tryptase protein in skin lesions. Moreover, Western blot was used to detect the protein levels of the MAPK/NF-κB signaling pathway. Results: Compared with the control group, the epidermis and spinous layer of rats in CE group were thickened, and a large amount of inflammatory cell infiltration was observed. The EASI score, serum IgE, His, IL-1β, TNF-α levels, number of mast cells, Tryptase, p-ERK/ERK, p-JNK/JNK, p-p38MARK/p38MARK, p-NF-κBp65/NF-κBp65 protein expression, and nuclear NF-κBp65 protein expression were prominently increased (P<0.05), while cytoplasmic NF-κBp65 protein expression was prominently decreased (P<0.05). Compared with CE group, SE-L group and SE-H group showed reduced thickening of the epidermis and spinous layer of the skin lesions in rats. The EASI score, serum IgE, His, IL-1β, TNF-α levels, number of mast cells, Tryptase, p-ERK/ERK, p-JNK/JNK, p-p38MARK/p38MARK, p-NF-κBp65/NF-κBp65 protein expression, and nuclear NF-κBp65 protein expression were conspicuously decreased (P<0.05), while cytoplasmic NF-κBp65 protein expression was conspicuously increased (P<0.05). Compared with SE-H group, H-SE+Anisomycin group showed increased damage to the skin lesion tissue. The EASI score, serum IgE, His, IL-1β, TNF-α levels, number of mast cells, Tryptase, p-ERK/ERK, p-JNK/JNK, p-p38MARK/p38MARK, p-NF-κBp65/NF-κBp65 protein expression, and nuclear NF-κBp65 protein expression were conspicuously increased (P<0.05), while cytoplasmic NF-κBp65 protein expression was conspicuously decreased (P<0.05). Conclusion: SE may improve skin damage in CE rats by inhibiting the MAPK/NF-κB signaling pathway. 2025 Vol. 31 (11): 1767-1772 [Abstract] ( 179 ) HTML (1 KB)  PDF (1935 KB)  ( 6 ) 1773 Mechanism of Tibial Transverse Bone Transfer on Trauma Repair in Diabetic Foot Rats Based on the NGF/Akt/GSK3β Pathway XU Kai, LIU Qizan, HE Da, et al DOI: 10.3969/j.issn.1006-6233.2025.11.03 Objective: To investigate the effect of tibial transverse bone relocation on trauma repair in diabetic foot rats, and to detect the expression of factors related to the NGF/Akt/GSK3β pathway to elucidate its possible mechanism of action. Methods: Forty SPF grade rats were selected, of which 10 were in the control group and the remaining 30 were modeled as diabetic foot rats. 28 rats finally completed the modeling and were divided into model group (10 rats), tibial transverse bone transfer group (9 rats), and tibial transverse bone transfer + MK-2206 group (9 rats). Wound healing rate, serum biochemical indexes, fasting blood glucose, oxidative stress indexes, capillary number, proportion of EPCs, VEGF, inflammatory factors and NGF/Akt/GSK3β pathway-related protein expression were compared between the groups. Results: Compared with the control group, TNF-α, fasting glucose, CRP, LDL-C, MDA, TG, IL-6, TC, and GSK3β protein expression were elevated, and the levels of Akt protein expression, capillary count, NGF protein expression, SOD, EPCs ratio, HDL-C, VEGF, and trauma healing rate in the model and tibia transverse bone relocation+MK-2206 group decreased, (P<0.05); Compared with the model group, the ratio of EPCs, SOD, NGF protein expression, capillary number, HDL-C, Akt protein expression, VEGF, and wound healing rate levels were increased, and the levels of TNF-α, MDA, LDL-C, fasting glucose, GSK3β protein expression, TC, CRP, TG, and IL-6 were decreased in the tibia transverse bone relocation group (P<0.05); Compared with the tibial transverse bone transfer group, the TNF-α, fasting glucose, IL-6, TC, MDA, CRP, TG, GSK3β protein expression, and LDL-C levels were increased in the tibial transverse bone transfer + MK-2206 group, and the levels of VEGF, SOD, Akt protein expression, HDL-C, NGF protein expression, the proportion of EPCs, the traumatic wound healing rate, and the level of the capillary number were decreased (P<0.05). Conclusion: Transverse tibial bone relocation promotes trauma repair and improves the expression of serum biochemical indexes, oxidative stress, capillary count, and inflammatory factors in diabetic foot rats, and its mechanism may be related to the NGF/Akt/GSK3β pathway. 2025 Vol. 31 (11): 1773-1779 [Abstract] ( 176 ) HTML (1 KB)  PDF (2149 KB)  ( 11 ) 1780 The Effect of Esketamine on Cognitive Function and Shh/Ptch1 Pathway in Rats with Obstructive Sleep Apnea Syndrome GU Kunfeng, MA Wennv, DONG Huiyong, et al DOI: 10.3969/j.issn.1006-6233.2025.11.04 Objective: To explore the effects of Esketamine (ESK) on hippocampal tissue injury and cognitive function in rats with obstructive sleep apnea syndrome (OSAS), as well as its regulatory role in the sonic hedgehog factor (Shh)/complement homologous 1 (Ptch1) pathway. Methods: Totally 72 male SD rats were stochastically separated into a control group, a model group (OSAS group), a low-dose ESK group (ESK-L group), a high-dose ESK group (ESK-H group), a Shh/Ptch1 pathway activator group (Purmorphoamine group), and a high-dose ESK+Shh/Ptch1 pathway inhibitor group (ESK-H+Cyclopamine group), each with 12 rats. Except for the control group, OSAS models were constructed in all other groups. The cognitive ability (Morris water maze test) and blood oxygen saturation (blood oxygen monitor) of rats in each group were tested. The hippocampal tissue of rats was taken to detect the inflammatory factors and oxidative stress factors (ELISA method), pathological changes in hippocampal tissue (HE staining), neuronal apoptosis (TUNEL method), neuronal ultrastructure (transmission electron microscopy method), and Shh/Ptch1 pathway proteins (Western blot method). Results: The OSAS group had prominently lower blood oxygen saturation, proportion of plateau dwelling time, IL-10 content, SOD activity, and Shh/Ptch1 pathway proteins in hippocampal tissue, and prominently higher escape latency, IL-6 and MDA contents in hippocampal tissue, and neuronal apoptosis rate (P<0.05). ESK could prominently increase blood oxygen saturation, proportion of plateau dwelling time, IL-10 content, SOD activity, and Shh/Ptch1 pathway proteins in hippocampal tissue, and prominently reduce escape latency, IL-6 and MDA contents in hippocampal tissue, and neuronal apoptosis rate. The changes in these indicators showed a dose-response relationship with ESK (P<0.05). Cyclopamine could reverse the improvement effects of ESK on hippocampal tissue damage and cognitive function in OSAS rats (P<0.05). Conclusion: ESK can inhibit inflammation and oxidative stress response in hippocampal tissue, alleviate neuronal apoptosis and pathological damage in hippocampal tissue, and improve cognitive ability. Its mechanism of action may be achieved by activating the Shh/Ptch1 pathway. 2025 Vol. 31 (11): 1780-1786 [Abstract] ( 166 ) HTML (1 KB)  PDF (2384 KB)  ( 8 ) 1787 Effects of Baicalein Modulation of the cAMP/CREB/BDNF Pathway on Autistic-Like Behaviour and Neuronal Damage in Autistic Rats ZHONG Wenwen, et al DOI: 10.3969/j.issn.1006-6233.2025.11.05 Objective: To explore the effects of baicalin on autistic-like behavior and neuronal damage in rats with autism by regulating cyclic adenosine monophosphate (cAMP)/cyclic adenosine monophosphate response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) pathway. Methods: A rat model of autism was established by intraperitoneal injection of valproic acid during pregnancy. The offspring were assigned into model group, baicalin group (100mg/kg), and baicalin+cAMP inhibitor (SQ22536) group (100mg/kg baicalin+2.22mg/kg SQ22536), with 12 rats in each group. The offspring of normal rats were considered as control group. The open field experiment was used to evaluate the spatial cognition and exploration ability of rats. Three-box experiments were used to test the social skills of rats. The self-combing experiment was used to test the stereotyped behavior of rats. Hematoxylin eosin (HE) and Nissl staining were used to observe the damage of rat hippocampal tissue and neurons. Enzyme linked immunosorbent assay (ELISA) was used to measure tumor necrosis factor-α(TNF-α), interleukin (IL)-1β, and IL-6 in rat hippocampal tissue. Western blot was used to detect cAMP, CREB, phosphorylated CREB (p-CREB), and BDNF proteins in rat hippocampal tissue. Results: For the control group, the hippocampal tissue structure of rats in the model group was damaged, the number of neuronal cells decreased, and the central region residence time, contact time with empty cages, combing frequency, and combing time raised, the TNF-α, IL-1β, IL-6 levels, and the cAMP, p-CREB, and BDNF proteins increased, but the number of upright times and contact time with unfamiliar mice decreased (P<0.05). For the model group, the hippocampal tissue structure and morphology of rats in the baicalin group were greatly improved, the number of neuronal cells raised, and the central region residence time, contact time with empty cages, combing frequency, and combing time reduced, the TNF-α, IL-1β, IL-6 levels, and the cAMP, p-CREB, and BDNF proteins decreased, but the number of upright times and contact time with unfamiliar mice increased (P<0.05). The cAMP inhibitor SQ22536 can reverse the improvement effect of baicalin on the above indicators in autistic rats (P<0.05). Conclusion: Baicalin may alleviate autism like behavior and neuronal damage in rats with autism by activating cAMP/CREB/BDNF pathway. 2025 Vol. 31 (11): 1787-1792 [Abstract] ( 166 ) HTML (1 KB)  PDF (1487 KB)  ( 11 ) 1793 Metformin Modulates HOXB7/β-Catenin Pathway to Inhibit Progression of Esophageal Squamous Cell Carcinoma KYSE-180 Cells LIU Shan, PAN Yue, ZHANG Zhuo, et al DOI: 10.3969/j.issn.1006-6233.2025.11.06 Objective: To investigate the inhibitory effect of metformin (MET) on the proliferation of esophageal squamous cell carcinoma (ESCC) KYSE-180 cells based on the homeobox protein B7 (HOXB7)/β-Catenin pathway. Methods: Human ESCC cells KYSE-180 were used as the research object. KYSE-180 cells were assigned into control group, MET-L group (0.5μmol/L), MET-H group (2μmol/L), MET-H+pcDNA-NC group (transfected with pcDNA-NC+2μmol/L MET), and MET-H+pcDNA-HOXB7 group (transfected with pcDNA-HOXB7+2μmol/L MET). Methyl thiazolyl tetrazolium (MTT) was used to detect cell proliferation. Transwell experiment and scratch assay were used to detect cell migration and invasion. Flow cytometry was used to detect the apoptosis rate of cells. In addition, real-time quantitative fluorescent PCR (qRT-PCR) and Western blot was used to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase (MMP)-9, HOXB7, and β-Catenin proteins in cells. A xenograft tumor mouse model was established to evaluate the anti-cancer effect of MET in vivo and its effect on HOXB7 and β-Catenin expression. Results: Compared with the control group, the MET-L group and MET-H group had lower HOXB7, β-Catenin mRNA expression, A490 value, cell migration number, scratch healing rate, cell invasion number, and PCNA, MMP-9, HOXB7, and β-Catenin protein expression (P<0.05), and higher apoptosis rate (P<0.05). Compared with the MET-H group and the MET-H+pcDNA-NC group, the MET-H+pcDNA-HOXB7 group had higher HOXB7, β-Catenin mRNA expression, A490 value, cell migration number, scratch healing rate, cell invasion number, and PCNA, MMP-9, HOXB7, and β-Catenin protein expression (P<0.05), and lower apoptosis rate (P<0.05). After MET treatment, tumor volume, tumor weight, HOXB7, β-Catenin protein expression decreased, TUNEL positive rate increased (P<0.05); overexpression of HOXB7 weakened the effect of MET on the above indexes (P<0.05). Conclusion: MET can inhibit the proliferation of ESCC KYSE-180 cells, and its mechanism may be achieved by inhibiting the HOXB7/β-Catenin pathway. 2025 Vol. 31 (11): 1793-1800 [Abstract] ( 177 ) HTML (1 KB)  PDF (3005 KB)  ( 5 ) 1801 The Effect of Neferine on Pain Response in Postherpetic Neuralgia Mice by Regulating the Gas6/Axl Signaling Pathway LEI Jian, HUANG Dan, GE Xingsen DOI: 10.3969/j.issn.1006-6233.2025.11.07 Objective: To explore the effect of neferine (NEF) on pain response in postherpetic neuralgia (PHN) mice by regulating the growth arrest specific protein 6 (Gas6)/AXL receptor tyrosine kinase (Axl) signaling pathway. Methods: PHN mice models were constructed. The successfully modeled mice were stochastically separated into PHN group, NEF-L group (5mg/kg), NEF-M group (10mg/kg), NEF-H group (20mg/kg), R428 group (20mg/kg and 125mg/kg Gas6/Axl signaling pathway inhibitor R428). Both NEF and R428 were administered via intraperitoneal injection, each with 10 mice. Another 10 normal C57BL/6J mice were served as the control group (NC group). The pain threshold and spontaneous foot contraction reflex frequency of mice were measured. HE staining was used to observe pathological changes in spinal cord tissue. ELISA was used to measure the inflammatory factors, prostaglandin E2 (PGE2), 5-hydroxytryptamine (5-HT), and substance P (SP) in mouse spinal cord tissue. TUNEL was used to observe apoptosis of spinal cord neurons in mice. Hexamine silver staining was used to observe morphological changes in mouse spinal cord tissue. Western blot was used to measure the Gas6/Axl signaling pathway and apoptosis related proteins in mouse spinal cord tissue. Results: The PHN group showed severe damage to the spinal cord tissue and neuronal fiber structure in mice, conspicuously higher spontaneous foot contraction reflex frequency, IL-6, IL-1β, PGE2, 5-HT, SP, neuronal apoptosis rate, Bax (P<0.05), and conspicuously lower mechanical pain threshold, thermal pain threshold, Gas6, p-Axl/Axl, Bcl-2 than the NC group (P<0.05). The NEF-L group, NEF-M group, and NEF-H group showed reduced damage to the spinal cord tissue and neuronal fiber structure in mice, lower spontaneous foot contraction reflex frequency, IL-6, IL-1β, PGE2, 5-HT, SP, neuronal apoptosis rate, Bax (P<0.05), and higher mechanical pain threshold, thermal pain threshold, Gas6, p-Axl/Axl, Bcl-2 than the PHN group (P<0.05). The R428 group showed aggravated damage to spinal cord tissue and neuronal fiber structure in mice, conspicuously higher spontaneous foot contraction reflex frequency, IL-6, IL-1β, PGE2, 5-HT, SP, neuronal apoptosis rate, Bax (P<0.05), and conspicuously lower mechanical pain threshold, thermal pain threshold, Gas6, p-Axl/Axl, Bcl-2 than the NEF-H group (P<0.05). Conclusion: NEF can improve pain and inflammatory response in PHN mice, which may be closely related to the activation of Gas6/Axl signaling pathway. 2025 Vol. 31 (11): 1801-1807 [Abstract] ( 156 ) HTML (1 KB)  PDF (2222 KB)  ( 11 ) 1808 Study on the Mechanism of KCNK1 Activating PI3K/AKT Signaling Pathway to Promote the Metastatic Ability of Papillary Thyroid Carcinoma HUANG Linchun, WANG Jianhua, LI Yiqing, et al DOI: 10.3969/j.issn.1006-6233.2025.11.08 Objective: To investigate the expression of potassium two pore domain channel subfamily K member 1 (KCNK1) in papillary thyroid cancer (PTC), and its relationship with clinical pathological parameters of patients, and its mechanism of promoting PTC cell metastasis. Methods: Totally 85 cases of cancer tissue and adjacent tissue removed from PTC patients in our hospital from January 2019 to December 2020 were collected. The expression of KCNK1 in PTC and adjacent tissues was detected by immunohistochemistry (IHC), to analyse the relationship between KCNK1 expression and patients’ clinical pathological parameters, prognosis. Western blot was used to detect the expression of KCNK1 in various PTC cell lines(KTC-1, IHH-4, TPC-1). PTC cell lines TPC-1 with high expression of KCNK1 were selected and divided into shNC group, shKCNK1-1 group, and shKCNK1-2 group. KCNK1 expression in each group of cells was detected by western blot. The cell metastasis ability of each group was detected by transwell experiment and scratch experiment. E-cadherin and N-cadherin protein expression in each group of cells was detected by western blot. Differentially expressed genes between the shNC group and shKCNK1-1 group were analyzed by whole transcriptome sequencing technology, and conducting KEGG signaling pathway enrichment analysis. The modulatory role of KCNK1 in regulating the PI3K/AKT signaling pathway was detected by western blot and functional experiments. Results: Compared with adjacent cancer tissues, the positivity rate of KCNK1 increased in PTC tissues (50.59% VS 21.18%;χ2=15.98,P=0.000). The positivity rate of KCNK1 increased in PTC patients with lymph node metastasis, cancer infiltration surrounding tissues, and advanced TNM staging (P<0.05). PTC patients with KCNK1 positive expression had a poorer prognosis (recurrence and metastasis rate 34.88% VS 14.29%;χ2=4.842,P=0.028). Compared with the normal human thyroid follicular epithelial cell line Nthyori3-1, KCNK1 expression was higher in PTC cell lines, and the highest expression was observed in TPC-1 cells (P<0.05). TPC-1 cells transfected with KCNK1 knockdown virus showed a decrease in KCNK1 expression in shKCNK1-1 and shKCNK1-2 cells compared to the shNC group (P<0.05), as well as a decrease in cell metastasis ability (P<0.05). The differentially expressed genes between the shNC group and the shKCNK1-1 group were concentrated within the PI3K/AKT signaling pathway revealed by the KEGG signaling pathway enrichment analysis. The expression of pPI3K and pAKT proteins was reduced in shKCNK1-1 cells compared to the shNC group (P<0.05). AKT agonist SC79 activates the PI3K/AKT signaling pathway activity in shKCNK1-1 group cells, which can weaken the inhibitory effect of knocking down KCNK1 on the metastatic ability of PTC cells. Conclusion: KCNK1 expression increases in PTC and is associated with clinical pathological parameters and poor prognosis. Knocking down KCNK1 inhibits the PI3K/AKT signaling pathway and suppresses the metastatic ability of PTC cells. 2025 Vol. 31 (11): 1808-1814 [Abstract] ( 166 ) HTML (1 KB)  PDF (2395 KB)  ( 10 ) 1815 Effects of Gentiopicroside on the Proliferation Migration and Invasion of Esophageal Squamous Cell Carcinoma by Regulating RhoA/ROCK1 Signaling Pathway YAN Chengyi, PENG Jianming, et al DOI: 10.3969/j.issn.1006-6233.2025.11.09 Objective: To explore the effects of gentiopicroside on the proliferation, migration, and invasion of esophageal squamous cell carcinoma by regulating Ras homolog family member A (RhoA)/rho A-associated coiled-coil kinase (ROCK1) signaling pathway. Methods: Human esophageal squamous cell carcinoma KYSE-180 cells were assigned into untreated group, L-gentiopicroside group, M-gentiopicroside group, H-gentiopicroside group, and H-gentiopicroside+N-arcilasine group. Colony formation assay and CCK-8 were performed to detect cell proliferation. The scratch healing experiment was performed to measure migration. Transwell experiment was performed to measure migration and invasion. Western blot was used to measure the RhoA and ROCK proteins in KYSE-180. Results: Compared with the untreated group, the L-gentiopicroside group, M-gentiopicroside group, and H-gentiopicroside group showed a decrease in OD450 value, scratch healing rate of KYSE-180 cells, the numbers of colony formation, migration, and invasive cells, and the RhoA and ROCK1 proteins (P<0.05), and showed a dose-dependent effect (P<0.05). Compared with the H-gentiopicroside group, the H-gentiopicroside+N-arcilasine group showed an increase in OD450 value, scratch healing rate of KYSE-180 cells, the numbers of colony formation, migration, and invasive cells, and the RhoA and ROCK1 proteins (P<0.05). Conclusion: Gentiopicroside inhibits the RhoA/ROCK1 signaling pathway and suppresses the proliferation, migration, and invasion of esophageal squamous cell carcinoma. 2025 Vol. 31 (11): 1815-1820 [Abstract] ( 161 ) HTML (1 KB)  PDF (2041 KB)  ( 11 ) 1821 Effects of Benzo[b]Fluoranthene on Renal Inflammation and AGE/RAGE Signaling Pathway in Rats with Chronic Kidney Disease XIAO Yuxin, WANG Hongying, et al DOI: 10.3969/j.issn.1006-6233.2025.11.010 Objective: To investigate the effects of benzo[b]fluoranthene (BbF) intervention on renal inflammation and the advanced glycation end products/receptor for advanced glycation end products (AGE/RAGE) signaling pathway in rats with chronic kidney disease (CKD). Methods: A total of 53 specific pathogen-free (SPF) male Sprague-Dawley (SD) rats were used. Fourteen rats were randomly selected as the control group, and the remaining 39 rats were used to establish a CKD model. Among them, 36 rats were successfully modeled and randomly divided into three groups: the model group (14 rats), the BbF group (11 rats), and the BbF + FPS-ZM1 group (11 rats). The body weight changes of rats in each group were compared before and after gavage. After 4 weeks of gavage, a balance was used to measure the weight of both kidneys and the kidney index; hematoxylin-eosin (HE) staining was used to detect pathological changes in renal tissue. Tail vein blood was collected after 4 weeks of gavage, and a biochemical analyzer was used to detect renal function indicators [serum creatinine (Scr), blood urea nitrogen (BUN), uric acid (UA)]. Renal tissue was collected after 4 weeks of gavage; enzyme-linked immunosorbent assay (ELISA) was used to detect the content of inflammatory factors [C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α)] and the levels of oxidative stress [superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px)]; flow cytometry was used to detect the expression levels of macrophage-related markers [CD11c+, CD206+, CD11c+/CD206+]; Western blotting (Wb) was used to detect the levels of the AGE/RAGE signaling pathway. Results: With the extension of gavage time, the body weight of rats in the control group showed a gradual increase trend, while the body weight of rats in the model group, BbF group, and BbF + FPS-ZM1 group all showed a decreasing trend, with a significant decrease in the BbF group (P<0.05). In addition, the pathological changes in the BbF group were obvious. Compared with the control group, model group, and BbF+FPS-ZM1 group, the BbF group had significantly increased levels of kidney weight, kidney index, UA, BUN, Scr, IL-6, CRP, TNF-α, IL-8, MDA, CD11c+/CD206+, CD11c+, RAGE mRNA, and AGE mRNA, while the levels of GSH-Px, SOD, and CD206+ were significantly decreased, with statistically significant differences (P<0.05). Conclusion: BbF can promote the development of inflammation in CKD rats, accelerate the progression of the disease by activating the AGE/RAGE signaling pathway, and at the same time have adverse effects on oxidative stress, renal function, and macrophages. 2025 Vol. 31 (11): 1821-1829 [Abstract] ( 169 ) HTML (1 KB)  PDF (1626 KB)  ( 13 ) 1830 Liraglutide Effects on Liver within High Fat Fed Obese Mice and the Effect in Repairing Alveolar Bone Defects BAI Xiangyu, HAO Yan, GUO Xiaoyu, et al DOI: 10.3969/j.issn.1006-6233.2025.11.011 Objective: To study the effects of liraglutide on the changes in oxidative stress levels in ob/ob mice and high-fat-fed ob/ob mice, and observe the changes in alveolar bone microstructure and the impact on bone metabolism. Methods:Wild type C57BL/6 mice were used as the control group (NC), and ob/ob mice were randomly divided into ob/ob group, ob/ob group+high-fat diet (HFD group), ob/ob+high-fat diet+liraglutide group (Lir group). After 8 weeks of feeding, body weight and body fat ratio were measured. HE staining was used to observe changes in liver cells and absorption of alveolar bone. The height of alveolar bone absorption at the cervical part of the tooth was measured under microscope. The expression of liver function,oxidative stress markers, and inflammatory factors was detected. Results: Compared with the NC group and ob/ob group, the HFD group showed more significant alveolar bone resorption in mice (P<0.05), while the Lir group showed some relief in alveolar bone resorption compared to the high-fat group (P<0.05). Compared with the NC group and ob/ob group, the HFD group showed an increase in inflammatory factors and an increase in oxidative stress markers in the periodontal tissue of mice (P<0.05), while the Lir group showed a significant decrease in inflammatory factors and a decrease in oxidative stress markers in the periodontal tissue of mice (P<0.05). Conclusion: Liraglutide can reduce lipid metabolism and liver lipid deposition in ob/ob mice fed with high-fat diet, decrease the accumulation of inflammatory factors, and improve alveolar bone resorption. Its mechanism may be related to the inhibition of periodontal oxidative stress. 2025 Vol. 31 (11): 1830-1834 [Abstract] ( 171 ) HTML (1 KB)  PDF (2283 KB)  ( 4 ) 1835 Analysis of Clinical Efficacy of 3D Modeling-Assisted Surgery in the Treatment of Gliomas in Functional Areas GAO Ningxi, SHI Qiuxia, YE Hualing, et al DOI: 10.3969/j.issn.1006-6233.2025.11.012 Objective: To compare the clinical efficacy of 3D modeling-assisted surgery and traditional craniotomy in the treatment of gliomas in functional areas, and to explore whether 3D modeling-assisted surgery has advantages. Methods: A total of 110 patients with gliomas admitted to our hospital from May 2022 to May 2025 were retrospectively enrolled and evenly assigned, according to the surgical approach, to an observation group (3D-modeling–assisted surgery, n = 55) or a control group (conventional craniotomy, n = 55). The two groups were compared in terms of operation duration, intraoperative blood loss, total tumor resection rate, KPS score of two weeks after surgery, serum inflammatory factor levels before and after surgery, clinical effective rate, complications, and adverse reactions. Results: In the observation group, the operation time, intraoperative blood loss, and length of hospital stay were all lower than those in the control group, while the resection rate and KPS score were higher than those in the control group, with significant differences (P<0.05). There was no significant difference in the overall incidence of complications between the two groups (P>0.05). TNF-α, IL-6, IL-1β and IFN-γ levels decreased from baseline in both the observation and control groups; the magnitude of change (pre- vs post-treatment) for every parameter was greater in the observation group than in the control group (P<0.05). Within each group, post-operative inflammatory mediator levels were significantly lower than pre-operative values (P<0.05). Baseline levels of IL-6, IL-1β and IFN-γ did not differ between the observation and control groups (P>0.05), whereas after treatment all three mediators were lower in the observation group than in the control group (P<0.05). The effective-response rate was higher in the observation group than in the control group (P<0.05), and the total incidence of adverse events was lower in the observation group (P<0.05). Conclusion: 3D modeling-assisted surgery shortens the operation time and hospital stay, reduces intraoperative blood loss, lowers the level of inflammatory factors, improves the resection rate of gliomas and the treatment efficiency of patients, and reduces the incidence of adverse reactions. It has the potential to be promoted and applied in clinical practice. 2025 Vol. 31 (11): 1835-1839 [Abstract] ( 175 ) HTML (1 KB)  PDF (1166 KB)  ( 12 ) 1840 Clinical Efficacy and Long-Term Follow-up Results of Laparoscopic Common Bile Duct Exploration alongside LC for the Treatment of Elderly Patients with Common Bile Duct Stones and Gallstones after Surgery PENG Qin, WANG Lin, DUAN Juanli, et al DOI: 10.3969/j.issn.1006-6233.2025.11.013 Objective: To evaluate the clinical efficacy and long-term follow-up results of laparoscopic common bile duct exploration and stone removal (LCBDE) alongside laparoscopic cholecystectomy (LC) in the treatment of elderly patients with common bile duct stones (CBDS) and gallstones. Methods: Elderly patients with CBDS and gallstones who received treatment in our hospital from March 2020 to March 2023 were divided into ERCP+LC group (n=55) and LCBDE+LC group (n=47) based on surgical methods. The two groups were compared in terms of surgical related indicators, postoperative complications, laboratory indicators, long-term complications, and recurrence rates. Results: The operation time, intraoperative blood loss, and postoperative anal exhaust time of the LCBDE+LC group were similar to those of the ERCP+LC group (P>0.05), the abdominal drainage time was longer than that of the ERCP+LC group, and the hospitalization time was shorter than that of the ERCP+LC group (P<0.05). The incidence of postoperative complications in the LCBDE+LC group was lower than that in the ERCP+LC group (P<0.05). The two groups did not exhibit statistically significant differences in terms of laboratory indicators and long-term complications within two years after the surgery (P>0.05). The recurrence rate in the LCBDE+LC group was lower than that in the ERCP+LC group (P<0.05). Conclusion: LCBDE combined with LC has a good therapeutic effect in elderly patients with CBDS and gallstones, which can promote patient recovery, shorten hospitalization time, and reduce the occurrence of short - and long-term complications and stone recurrence. 2025 Vol. 31 (11): 1840-1843 [Abstract] ( 173 ) HTML (1 KB)  PDF (1150 KB)  ( 14 ) 1844 Analysis of the Relationship of Serum Cardiac Troponin Levels with Clinicopathological Parameter Characteristics and Prognosis in Patients with Lung Cancer SUN Ruijie, WU Chunming, WANG Yimin DOI: 10.3969/j.issn.1006-6233.2025.11.014 Objective: To analyze the relationship of serum cardiac troponin I(cTnI) level with clinicopathological characteristics and prognosis in patients with lung cancer, and to evaluate the predictive value of cTnI on the prognosis. Methods: A total of 107 patients with lung cancer who received chemotherapy in the hospital from January 2020 to January 2023 were classified into a good prognosis group (n=50) and a poor prognosis group (n=57) according to the prognosis status within 1 year. After the end of chemotherapy, the serum cTnI levels of all patients were measured. The serum cTnI levels of patients with different pathological characteristics and prognosis were compared, and the predictive value of serum cTnI levels for the prognosis of patients was analyzed by ROC curve. Results: Serum cTnI level was correlated with lymph node metastasis and TNM staging (P<0.05), and there was no correlation with age, sex, pathological type, tumor diameter, or depth of invasion (P>0.05). The serum cTnI level in the poor prognosis group was higher than that in the good prognosis group (P<0.05). The ROC curve shows that the area under the curve (AUC) of serum cTnI level for predicting poor prognosis in lung cancer patients is 0.862, with a sensitivity of 66.67% and a specificity of 96.00%. Conclusion: The level of cTnI in patients with lung cancer undergoing chemotherapy is closely related to lymph node metastasis, TNM staging, and prognosis. The determination of serum cTnI level in the early stage of chemotherapy can provide predictive value on the prognosis of patients. 2025 Vol. 31 (11): 1844-1848 [Abstract] ( 171 ) HTML (1 KB)  PDF (1356 KB)  ( 2 ) 1849 The Expression of miR-182-3p miR-195-5p miR-200a-3p in the Serum of Patients with Hypertensive Disorders of Pregnancy and the Predictive Value for Premature Birth ZHEN Feifei, YAN Cuiyun, WEI Tao DOI: 10.3969/j.issn.1006-6233.2025.11.015 Objective: To investigate the expression of microRNAs (miRNAs)-182-3p, miR-195-5p, and miR-200a-3p in the serum of patients with hypertensive disorders of pregnancy (HDP) and their predictive value for premature birth. Methods: A total of 391 patients with HDP treated in the obstetrics and gynecology department of our hospital from June 2022 to June 2024 were regarded as study group. Complying with severity of the disease, they were classified into 165 cases of simple HDP, 134 cases of mild preeclampsia, and 92 cases of severe preeclampsia. Complying with occurrence of premature birth in patients, they were grouped into a premature birth group of 107 cases (28 weeks ≤ gestational time<37 weeks) and a non premature birth group of 284 cases (37 weeks ≤ gestational time<42 weeks). During the same period, 316 healthy pregnant women who gave birth at full term were made the control group. Real-time fluorescence quantitative PCR was used to detect serum miR-182-3p, miR-195-5p, and miR-200a-3p. Multivariate logistic regression was used to analyze the factors influencing premature birth in patients with HDP. Receiver operating characteristic curves (ROC) were used to analyze the predictive value of serum miR-182-3p, miR-195-5p, and miR-200a-3p for premature birth in HDP patients. Results: Compared with control group, study group had higher serum miR-182-3p and miR-200a-3p, and lower miR-195-5p (P<0.05). Serum miR-182-3pp and miR-200a-3p in patients with severe preeclampsia were higher than those in patients with mild preeclampsia and simple HDP (P<0.05), while miR-195-5 was lower (P<0.05). The systolic blood pressure, diastolic blood pressure, proportion of severe preeclampsia, 24-hour UPQ, Scr, serum miR-182-3p, and miR-200a-3p in premature birth group were higher than those in non premature birth group (P<0.05), while serum miR-195-5p was lower than that in non premature birth group (P<0.05). The severity of the disease, 24h UPQ, serum miR-182-3p, miR-195-5p, and miR-200a-3p were factors that affected premature birth in HDP patients (P<0.05). The AUC of the joint of serum miR-182-3p, miR-195-5p, and miR-200a-3p in predicting premature birth in HDP patients was 0.903, and the joint of the three was better than their individual predictions P<0.05). Conclusion: The levels of miR-182-3p and miR-200a-3p in serum of HDP patients are elevated, while the level of miR-195-5p is reduced. The joint of the three has certain clinical application value in predicting premature birth in HDP patients. 2025 Vol. 31 (11): 1849-1854 [Abstract] ( 160 ) HTML (1 KB)  PDF (1355 KB)  ( 5 ) 1855 Efficacy of Median Nerve Electrical Stimulation Combined with Cerebroprotein Hydrolysate on Disturbance of Consciousness in Patients with Craniocerebral Injury and Its Influence on Hcy NSE and BDNF Levels ZHANG Jingru, PAN Jianping, TANG Wenyu, et al DOI: 10.3969/j.issn.1006-6233.2025.11.016 Objective: To analyze the efficacy of median nerve electrical stimulation combined with cerebroprotein hydrolysate on disturbance of consciousness in patients with craniocerebral injury and its influence on the levels of homocysteine (Hcy), neuron-specific enolase (NSE), and brain-derived neurotrophic factor (BDNF). Methods: Totally 114 patients with craniocerebral injury admitted to the hospital from April 2022 to October 2024 were selected and divided into cerebroprotein group (single number, n=57) and electrical stimulation group (double number, n=57) by the random number table method. On the basis of conventional treatment, the cerebroprotein group was treated with a mixture of 10 mL of brain protein hydrolysate and 250 mL of 0.9% sodium chloride injection, and the electrical stimulation group was treated with median nerve electrostimulation on the basis of the cerebroprotein group. Both groups were treated for 4 weeks. The clinical efficacy, electromyography, awake rate, coma status, NIHSS score, cerebral hemodynamic indicators [mean arterial blood flow velocity (Vm), cerebral blood volume (CBV), cerebral blood flow (CBF)], inflammatory factors and lipid peroxide indicators [interleukin-6 (IL-6), malondialdehyde (MDA), superoxide dismutase (SOD)] and serum biological indicators (Hcy, NSE, BDNF) before and after treatment were compared between groups. The adverse drug reactions and adverse electrical stimulation events in the two groups were counted during treatment. Results: The total effective rate of treatment in electrical stimulation group was higher than that in cerebroprotein group (P<0.05). After treatment, the latency, M wave latency and MCV in the two groups were significantly improved, and the improvements in electrical stimulation group were more obvious than those in cerebroprotein group (P<0.05). The awake rate of electrical stimulation group after treatment was significantly higher than that of cerebroprotein group (P<0.05). The GCS score and NIHSS score in both groups were significantly improved, and the improvements were more obvious in electrical stimulation group (P<0.05). After treatment, the Vm, CBV and CBF were significantly improved in the two groups, and the improvements in electrical stimulation group were more significant compared to cerebroprotein group (P<0.05). After treatment, the levels of IL-6, MDA, and SOD in both groups were significantly improved, and the improvement was more pronounced in the electrical stimulation group than in the cerebroprotein group (P<0.05). After treatment, the levels of Hcy, NSE, and BDNF in both groups were also significantly improved, with the electrical stimulation group showing more significant improvement than the cerebroprotein group (P<0.05). There were no significant differences in the adverse drug reactions and adverse electrical stimulation events between groups (P>0.05). Conclusion: Median nerve electrical stimulation combined with cerebroprotein hydrolysate has obvious efficacy on disturbance of consciousness in patients with craniocerebral injury. It can improve the neurological deficit, relieve the body’ s inflammatory factors, promote the cerebral hemodynamics and neurological function recovery, and it is safe and effective. 2025 Vol. 31 (11): 1855-1861 [Abstract] ( 149 ) HTML (1 KB)  PDF (1612 KB)  ( 3 ) 1862 Analysis of the Correlation and Predictive Value of Cytokine Levels with Postoperative Recurrence and Bone Metastasis in Patients with Prostate Cancer after Radical Prostatectomy ZHANG Yanhong, CHEN Na, WANG Wei, et al DOI: 10.3969/j.issn.1006-6233.2025.11.017 Objective: To investigate the correlation between cytokine levels and postoperative recurrence and bone metastasis in prostate cancer (PCa) patients, as well as their clinical predictive value. Methods: A retrospective collection of clinical data from 134 patients with prostate cancer who underwent radical prostatectomy (RP) from August 2021 to August 2024 was conducted. These patients were divided into a recurrence group (54 cases) and a non-recurrence group (80 cases) based on whether they had recurrence. Among the recurrence group, patients were further divided into a bone metastasis group (23 cases) and a non-bone metastasis group (31 cases) according to the type of recurrence. Multivariate Logistic regression model was used to screen the influencing factors of bone metastasis in patients after RP, and ROC curve was used to evaluate the predictive efficacy of related indicators. Results: The preoperative levels of prostate-specific antigen (PSA) and Leptin, postoperative Gleason score, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), and tumor necrosis factor-α (TNF-α) in the recurrence group were higher than those in the non-recurrence group (P<0.05). Among the patients with recurrence, those in the bone metastasis group had significantly higher preoperative PSA levels, Leptin levels, and postoperative levels of transforming growth factor-β1 (TGF-β1), IL-6, and TNF-α compared with the non-bone metastasis group (P<0.05). The results of multivariate logistic regression analysis showed that postoperative TNF-α level was an independent influencing factor (P <0.05). ROC curve analysis showed that the AUC of TNF-α for predicting bone metastasis was 0.915. At the cut-off value of 15.52 pg/mlL, the sensitivity was 90.3%, the specificity was 91.3%, the positive predictive value was 87.5%, the negative predictive value was 93.3%, and the accuracy was 90.7%. Conclusion: Postoperative serum TNF-α level is an independent predictive indicator for postoperative recurrent bone metastasis in PCa patients, with high clinical application value, and can be used as an important biological marker for evaluating the risk of postoperative bone metastasis. 2025 Vol. 31 (11): 1862-1867 [Abstract] ( 165 ) HTML (1 KB)  PDF (1287 KB)  ( 8 ) 1868 Correlation between ET-1 MDA MMP-9 in Serum and Tears and Severity of Type 2 Diabetes Mellitus with Dry Eye Disease MA Xiaoyu, ZHANG Lu, LU Silin, et al DOI: 10.3969/j.issn.1006-6233.2025.11.018 Objective: To explore the correlation between serum and tear endothelin-1 (ET-1), malondialdehyde (MDA), matrix metalloproteinase-9 (MMP-9) and the severity of type 2 diabetes mellitus (T2DM) with dry eye disease (DED). Methods: Totally 136 patients with T2DM admitted to the general hospital of central theater command from September 2023 to September 2024 were selected and divided into DED group (54 cases) and non-DED group (82 cases) according to whether they were accompanied by DED. Another 120 healthy individuals of the same age range who passed the health examination were made the control group. Complying with the evaluation results of the disease, the DED group was assigned into the mild group of 24 cases and the moderate/severe group of 30 cases. The risk factors for moderate/severe DED in T2DM patients were analyzed using multiple logistic regression. ROC was used to analyze the diagnostic value of serum and tear ET-1, MDA, and MMP-9 for moderate/severe DED in T2DM. Results: The DED group and non-DED group had prominently higher serum and tear ET-1, MDA, and MMP-9 than control group, meantime, the DED group had prominently higher results than the non-DED group (P<0.05). The moderate/severe group showed obvious upregulation of serum and tear ET-1, MDA, and MMP-9 expression than mild group (P<0.05). Elevated levels of ET-1, MDA, and MMP-9 in serum and tears were independent risk factors for moderate/severe DED in T2DM (P<0.05). The AUC of joint of serum ET-1, MDA, and MMP-9 in the diagnosis of moderate/severe DED in T2DM was 0.981, and the AUC of joint diagnosis was better than that of single diagnosis (Z=2.231, 2.325, 2.508, P<0.05). The AUC of joint diagnosis of ET-1, MDA, and MMP-9 in tears for moderate/severe DED in T2DM was 0.983, and the AUC of joint diagnosis was better than that of single diagnosis (Z=2.105, 2.457, 2.751, P<0.05). Conclusion: The ET-1, MDA, and MMP-9 in the serum and tears of T2DM patients with DED are upregulated, and they are related to condition of DED. The joint diagnosis for moderate/severe DED in T2DM by the three methods has certain value. 2025 Vol. 31 (11): 1868-1873 [Abstract] ( 160 ) HTML (1 KB)  PDF (1409 KB)  ( 16 ) 1874 The Clinical Values and Dynamic Changes of Serum ACE2 and IL-18 in Patients with Severe Acute Pancreatitis LIU Shengyi, YAO Ting, GU Zhiyi, et al DOI: 10.3969/j.issn.1006-6233.2025.11.019 Objective: To explore the dynamic changes of serum angiotensin-converting enzyme 2 (ACE2) and interleukin-18 (IL-18) in patients with severe acute pancreatitis (SAP) and their clinical values. Methods: A total of 150 SAP patients from January 2021 to December 2024 were prospectively selected as the research objects and recorded as the SAP group. Meanwhile, 150 healthy volunteers who underwent physical examinations in our hospital during the same period were selected as the control group. According to the prognosis of SAP patients, they were divided into the good prognosis group (n=107) and the poor prognosis group (n=43). The levels of serum ACE2 and IL-18 were detected by enzyme-linked immunosorbent assay (ELISA). The Bedside Index of Severity in Acute Pancreatitis (BISAP) score, the Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) score, and the Ranson score were used to evaluate the disease severity of SAP patients. Pearson correlation analysis was performed to analyze the correlation between the levels of ACE2 and IL-18 and the severity of SAP. The receiver operating characteristic (ROC) curve was used to evaluate the predictive value of the levels of ACE2 and IL-18 for the poor prognosis of SAP patients, and Z-test was used to compare the differences in the area under the curve (AUC). Results: Compared with the control group, the level of ACE2 (on the first day) in the SAP group was lower, while the level of IL-18 (on the first day) was higher (P<0.05). On the 1st, 3rd, and 7th days after admission, the levels of ACE2 in SAP patients gradually increased, while the levels of IL-18 gradually decreased (P<0.05). The BISAP score, APACHE Ⅱ score, and Ranson score in the poor prognosis group were all higher than those in the good prognosis group (P<0.05). On the 1st, 3rd, and 7th days after admission, the levels of ACE2 in the poor prognosis group were lower than those in the good prognosis group, and the levels of IL-18 were higher than those in the good prognosis group. Meanwhile, the serum levels of ACE2 in both groups gradually increased, and the levels of IL-18 gradually decreased (P<0.05). In the early stage of the disease (on the first day), the level of ACE2 was negatively correlated with the BISAP score, APACHE Ⅱ score, and Ranson score, while the level of IL-18 was positively correlated with the BISAP score, APACHE Ⅱ score, and Ranson score (P<0.05). In the early stage of the disease, the AUC of the combined prediction of the poor prognosis of SAP patients by ACE2 and IL-18 was 0.882, which was better than that of the individual detection (0.744, 0.761) (all P<0.05). Conclusion: The level of ACE2 in SAP patients is relatively low and gradually increases with the extension of treatment time, while the level of IL-18 is relatively high and gradually decreases with the extension of treatment time. Both are correlated with the disease severity. The combined prediction of the poor prognosis of SAP patients has certain clinical value and provides an effective reference for the prognosis evaluation of patients. 2025 Vol. 31 (11): 1874-1879 [Abstract] ( 158 ) HTML (1 KB)  PDF (1300 KB)  ( 17 ) 1880 Analysis of the Effect and Efficacy of Endoscopic Combined with Drug/Interventional Therapy on MAP CVP and Blood Lactate Levels in Patients with Upper Gastrointestinal Bleeding and Hemorrhagic Shock GAN Xiaojuan, XU Shanshan, GAO Fengjuan, et al DOI: 10.3969/j.issn.1006-6233.2025.11.020 Objective: To explore the influence and efficacy of endoscopy combined with drug/interventional therapy on MAP, CVP and blood lactate levels in patients with upper gastrointestinal bleeding and hemorrhagic shock. Methods: A total of 135 patients with hemorrhagic shock caused by upper gastrointestinal bleeding who were treated in the First Affiliated Hospital of Xinjiang Medical University (Emergency and Trauma Center) from February 2022 to February 2024 were selected and randomly divided into a control group of 71 cases and a combined group of 64 cases. The control group received endoscopic treatment alone, while the combined group received endoscopic treatment combined with drug/interventional therapy. The hemostasis time,fluid infusion volume, prothrombin time, hospital stay, clinical efficacy, hemodynamic indicators and metabolic indicators (mean arterial pressure, central veno-us pressure, blood lactate), rebleeding rate, and complication rate (perforation, infection, thrombosis) were compared between the two groups. Results: The combined group demonstrated a higher total effective rate compared to the control group. Meanwhile, indicators including hemostasis duration, fluid infusion volume, prothrombin time, hospitalization stay length, rebleeding rate, and complication incidence were all lower in the combined group than in the control group. The MAP and blood lactate levels of patients in the combined group were decreased, and the CVP level was increased (P<0.05). The difference in MAP and CVP before and after treatment in the combined group was higher than that in the control group (P<0.05). The difference in blood lactate levels before and after treatment in the combined group was higher than that in the control group, but the difference was not statistically significant (P>0.05). Conclusion: Endoscopic treatment combined with drug/interventional therapy can effectively control bleeding, improve coagulation and metabolic status, reduce risks, and ensure patient safety. 2025 Vol. 31 (11): 1880-1883 [Abstract] ( 159 ) HTML (1 KB)  PDF (1146 KB)  ( 9 ) 1884 Relationship and Clinical Value of Heparin-Binding Protein and Six Items of Leukocyte Differentiation Antigens with Disease Severity and Prognosis of Pediatric Pneumonia in Preschool Children CHEN Feifei, ZHU Feng, WANG Zongyan DOI: 10.3969/j.issn.1006-6233.2025.11.021 Objective: To explore the relationship between heparin-binding protein (HBP) and 6 items of leukocyte differentiation antigens and disease severity and prognosis in preschool children with pediatric pneumonia. Methods: Totally 120 preschool children with pediatric pneumonia admitted to the hospital from November 2023 to October 2024 were selected as research subjects, including 86 cases in the mild group and 34 cases in the severe group. According to the prognosis status, the enrolled patients were classified into good prognosis group (95 cases) and poor prognosis group (25 cases). Serum HBP and 6 items of leukocyte differentiation antigens [T lymphocyte (CD3%), helper/inducible T cell (CD4%), suppressor/killer T cell (CD8%), CD4/CD8, B lymphocyte (CD19%), NK cell (CD16+CD56%)] were measured in all subjects. The relationship of HBP and leukocyte differentiation antigens with disease severity and prognosis in preschool children with pneumonia was explored by Logistic regression analysis. ROC curve was adopted to analyze the evaluation value of HBP and leukocyte differentiation antigens on disease condition and prognosis. Results: Serum HBP level in the severe group was higher while the levels of CD3%, CD4%, CD4/CD8, CD19% and CD16+CD56% were lower than those in the mild group (P<0.05). There was no statistically significant difference in the CD8% level between the two groups (P>0.05). The level of serum HBP in the poor prognosis group was higher than that in the good prognosis group while the levels of CD3%, CD4%, CD4/CD8, CD19% and CD16+CD56% were lower (P<0.05). There was no statistically significant difference in the CD8% level between the two groups (P>0.05). Logistic regression analysis suggested that elevated serum HBP level and decreased CD3%, CD4%, CD4/CD8, CD19% and CD16+CD56% were risk factors of disease severity and poor prognosis in preschool children with pediatric pneumonia (P<0.05). ROC curve found that the AUC, sensitivity and specificity of combination of serum HBP, CD3%, CD4%, CD4/CD8, CD19% and CD16+CD56% were 0.935, 100.00% and 79.07% in evaluating the disease severity of children, and were 0.948, 92.00% and 90.53% in evaluating the prognosis of children. The efficiency of combined evaluation was better than that of each indicator alone (P<0.05). Conclusion: HBP and leukocyte differentiation antigens are closely associated with the disease severity and prognosis in preschool children with pediatric pneumonia. The combined determination of various indicators can provide reference value for clinical evaluation of disease severity and prognosis of children. 2025 Vol. 31 (11): 1884-1890 [Abstract] ( 169 ) HTML (1 KB)  PDF (1507 KB)  ( 10 ) 1891 Application Effect and Safety of Ultrasound-Guided Continuous Femoral Nerve Block for Analgesia in Patients Undergoing Knee Surgery SHEN Yuxiu, XU Xiangsheng, et al DOI: 10.3969/j.issn.1006-6233.2025.11.022 Objective: To analyze the effect and safety of ultrasound-guided continuous femoral nerve block (CFB) for analgesia in knee surgery. Methods: From March 2021 to June 2024, 103 patients with knee injuries who underwent knee surgery in the hospital were selected as the study subjects. They were grouped by random number table method. The control group (n=51) received patient-controlled intravenous analgesia (PCIA), while the observation group (n=52) received ultrasound-guided CFB for analgesia. The two groups were compared on analgesic effect [Visual Analogue Scale (VAS) score for pain], inflammatory response indicators [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP)], the incidence of postoperative delirium, and adverse reactions. Results: Compared with the control group, the observation group showed reductions in VAS in resting state at 8h, 12h, 24h, and 48 h after surgery and VAS in the motion state at 4h, 8h, 12h, 24h, and 48h after surgery (P<0.05). After surgery, the levels of inflammatory markers in both groups increased, and the levels were lower in observation group (P<0.05). Delirium was observed in the control group on the 1st, 2nd, and 3rd day after surgery, and in the observation group on the 2nd day. No delirium was observed in the two groups on the 4th, 5th, 6th, and 7th day after surgery. The total incidence of delirium was lower in the observation group (P<0.05). Both groups showed an increase in knee range of motion (KSS) scores after surgery. At 24h and 49h after surgery, the observation group had higher KSS scores than the control group (P<0.05). Two (3.85%) patients in the observation group and ten (19.61%) in the control group received additional use of analgesics. There were fewer patients receiving remedial analgesia in the observation group compared with the control group (χ2=6.214, P=0.013). The total incidence of adverse reactions was 19.23% in the observation group, lower than 49.02% in the control group (P<0.05). Conclusion: Ultrasound-guided CFB can achieve a good analgesic effect in patients undergoing knee surgery. It can inhibit inflammatory response and reduce the occurrence of delirium and adverse reactions. 2025 Vol. 31 (11): 1891-1895 [Abstract] ( 162 ) HTML (1 KB)  PDF (1167 KB)  ( 4 ) 1896 Hemostatic Effect of Endoscopic Tissue Glue Injection Combined with Titanium Clip in the Treatment of Acute Non-Variceal Upper Gastrointestinal Hemorrhage DUAN Zhengang, JING Yujie, ZHUO Wangqiang, et al DOI: 10.3969/j.issn.1006-6233.2025.11.023 Objective: To explore the hemostatic effect of endoscopic tissue glue injection combined with titanium clip in treating acute non-variceal upper gastrointestinal hemorrhage (ANVUGIB). Methods: The data of patients with ANVUGIB from January 2023 to February 2024 were retrospectively analyzed, and the above patients were divided into titanium clip group (titanium clip therapy) and titanium clip tissue adhesive group (endoscopic tissue glue injection on the basis of the treatment regimen in titanium clip assembly) according to treatment methods. The two groups of patients were excluded from the influence of confounding factors of baseline data according to the propensity score matching method (caliper=0.02), and 51 patients with comparable baseline data were finally obtained in each group. The hemostatic effect, improvement times of symptoms such as drainage clearing, occult blood negative conversion and hematemesis disappearance, complications, blood indicators [hemoglobin (Hb), red blood cell count (RBC), prothrombin time (PT)] before and after treatment and re-hemorrhage within half a year were compared between the two groups. Results: The success rate of 72 h hemostasis was 96.08% in titanium clip tissue adhesive group and that in titanium clip group (84.31%), with a statistically significant difference (P<0.05). The improvement times of symptoms such as drainage clearing, occult blood negative conversion and hematemesis disappearance were shorter in titanium clip tissue adhesive group than those in titanium clip group, with statistically significant differences (P<0.05). There was no statistically significant difference in total incidence rate of complications between titanium clip tissue adhesive group (5.88%) and titanium clip group (13.73%) (P>0.05). After surgery, Hb and RBC were increased in both groups (P<0.05), and PT was reduced, the differences were statistically significant (P<0.05), and the differences of various indicators in titanium clip tissue adhesive group before and after surgery were statistically different than those in titanium clip group (P<0.05). The re-hemorrhage rate within half a year in titanium clip tissue adhesive group was 1.96% and that in titanium clip group was 13.73%, with a statistical difference (P<0.05). Conclusion: Endoscopic tissue glue injection combined with titanium clip can effectively treat ANVUGIB, and improve clinical symptoms and blood indicators of patients, and it has few complications and low re-hemorrhage rate, and it is safe and effective. 2025 Vol. 31 (11): 1896-1900 [Abstract] ( 165 ) HTML (1 KB)  PDF (1178 KB)  ( 5 ) 1901 Study on the Relationship between Quantitative Parameters of MSCT and TIMP-1 HIF-1α and Their Diagnostic Value for COPD ZHENG Guoli, LIU Yan, BU Chunhong DOI: 10.3969/j.issn.1006-6233.2025.11.024 Objective: To investigate the value of quantitative multislice spiral CT (MSCT) parameters combined with serum tissue-type inhibitor of metalloproteinase-1 (TIMP-1) and hypoxia-inducible factor-1α (HIF-1α) in the diagnosis and management of chronic obstructive pulmonary disease (COPD). Methods: A total of 83 suspected COPD patients diagnosed in our hospital from September 2021 to September 2023 were selected. After pathological examination, as well as lung function and chest CT, 52 patients were confirmed to have COPD and were classified as the observation group, while the remaining 31 patients were classified as the control group. All underwent MSCT quantitative examination. The quantitative parameters of MSCT were calculated, including the ratio of wall thickness to diameter (TDR), lumen area (Ai), the ratio of wall thickness to body surface area (T/BSA), and the percentage of bronchial wall area (WA%). The levels of TIMP-1 and HIF-1α were detected. Multivariate Logistic regression analysis was conducted to analyze the independent risk factors influencing the occurrence of COPD. The correlation between the quantitative parameters of MSCT and TIMP-1 and HIF-1α was analyzed. The correlation between TIMP-1, HIF-1α and different severity levels of the disease were analyzed. Results:Compared with the control group, the parameter levels of TIMP-1, HIF-1α, TDR, T/BSA and WA% in the observation group increased, while the Ai level decreased (P<0.05). The parameter levels of TDR, T/BSA and WA% in severe patients were higher than those in mild and moderate patients, while the Ai level was lower than that in mild and moderate patients (P<0.05). Compared with mild and moderate patients, the levels of TIMP-1 and HIF-1α in severe patients were both increased (P<0.05). The results of multivariate Logistic regression analysis showed that the independent risk factors influencing the occurrence of COPD included TDR, HIF-1α, T/BSA, Ai, TIMP-1, and WA%. TDR, T/BSA and WA% are positively correlated with the severity of the disease. The level of Ai was negatively correlated with the severity of the disease, while the levels of TIMP-1 and HIF-1α were positively correlated with the severity of the disease (P<0.05). TDR, T/BSA, WA% were positively correlated with TIMP-1 and HIF-1α (P<0.05). Ai was negatively correlated with the levels of TIMP-1 and HIF-1α (P<0.05). The efficacy of the combined diagnosis was significantly higher than that of the individual diagnoses of TIMP-1 and HIF-1α. Conclusion:The correlation between the quantitative parameters of multilayer spiral CT and TIMP-1, HIF-1 α levels and different disease severity, which improves the clinical application in chronic obstructive pulmonary disease. 2025 Vol. 31 (11): 1901-1906 [Abstract] ( 151 ) HTML (1 KB)  PDF (1297 KB)  ( 8 ) 1907 Study on HBV-DNA Load Serum Surface Antigen miR-122 and miR-21 Expression Levels and Their Correlation in Patients with Chronic Hepatitis B LIU Lina, LIANG Yonggang, LI Li DOI: 10.3969/j.issn.1006-6233.2025.11.025 Objective: To investigate the hepatitis B virus (HBV)-DNA load and expression levels of serum surface antigen (HBsAg), miR-122 and miR-21 in patients with chronic hepatitis B (CHB) and study their correlation. Methods: A total of 120 CHB patients admitted from December 2019 to July 2024 were selected and divided into low load group (n=52) and high load group (n=68) according to different HBV-DNA loads, and the HBV-DNA load and expression levels of HBsAg, miR-122 and miR-21 were compared between groups. Pearson method was used to analyze the correlation between HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels. The patients were classified into mild group (n=48) and moderate-to-severe group (n=72) according to the inflammation grading of liver histopathological diagnostic criteria, and the HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels were compared. Binary logistics equation analysis was used to analyze the related influencing factors affecting the inflammatory activity of liver tissue. ROC curve was used to analyze the diagnostic value of HBV-DNA load, HBsAg, miR-122 and miR-21 on the inflammatory activity of liver tissue. Results: The miR-122 and miR-21 in high load group were significantly higher than those in low load group (P<0.05). Pearson correlation analysis showed that the expression levels of HBsAg, miR-122 and miR-21 were positively correlated with HBV-DNA load (P<0.05). The HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels were significantly higher in moderate-to-severe group than those in mild group (P<0.05). The results of multiple logistic regression analysis show that HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels were correlated with hepatitis activity (P<0.05). The AUCs of HBV-DNA load, HBsAg, miR-122 and miR-21 in predicting liver tissue inflammatory activity were 0.918, 0.706, 0.768, and 0.749, respectively. The AUC of the four combined diagnosis of liver tissue inflammatory activity was 0.960, and the combined diagnostic value was higher. Conclusion: Severe CHB is characterized by high HBV-DNA load, serum surface antigen, miR-122 and miR-21, and these indicators are expected to be biomarkers for predicting inflammatory activity of liver tissue. 2025 Vol. 31 (11): 1907-1912 [Abstract] ( 166 ) HTML (1 KB)  PDF (1368 KB)  ( 5 ) 1913 Clinical Study on the Treatment of Lumbar Spinal Stenosis with Percutaneous J-Shaped Micro Bone File Combined with Low-Temperature Plasma Target Ablation Guided by C-Arm Machine SHAN Dong, GAO Shangming, WANG Yunlong, et al DOI: 10.3969/j.issn.1006-6233.2025.11.026 Objective: To observe the clinical efficacy of percutaneous J-shaped micro bone file combined with low-temperature plasma target ablation guided by C-arm machine in the treatment of lumbar spinal stenosis. Methods: Totally 150 patients with lumbar spinal stenosis who received treatment at the First People's Hospital of Huai'an from July 2023 to June 2024 were selected. Randomly divided using a table of random numbers, the patients were divided into a research group (treatment group with J-shaped micro bone file combined with low-temperature plasma target ablation, 75cases) and a control group (treatment group with J-shaped micro bone file, 75cases). The two groups were compared in terms of VAS scores, lumbar range of motion, ODI scores, JOA scores, and the pre- and post-treatment differences, as well as the effectiveness and safety of the two treatments. Results: After treatment, the VAS scores and ODI scores of both groups were significantly lower than those before treatment (P < 0.05), and the research group had a greater reduction in VAS and ODI scores than the control group (P < 0.05). The lumbar extension, lumbar flexion degrees and JOA scores of both groups were significantly higher than those before treatment (P < 0.05), and the increases in lumbar extension, lumbar flexion, and JOA scores were greater in the study group than in the control group (P<0.05). Comparison of the overall effective rate shows the effective rate of 93.33% in the research group and 86.67% in the control group, with no statistically significant difference(P＞0.05). No adverse reactions occurred in either group. Conclusion: Under the guidance of C-arm machine, percutaneous J-shaped micro bone file combined with low-temperature plasma target ablation is effective and safe in treating lumbar spinal stenosis, they have significant advantages in relieving pain, improving lumbar spine mobility, and enhancing lumbar spine function. 2025 Vol. 31 (11): 1913-1918 [Abstract] ( 156 ) HTML (1 KB)  PDF (2739 KB)  ( 5 ) 1919 Correlation of SP1 and UBE2C Expression with Clinicopathological Features in Esophageal Squamous Carcinoma JIANG Haibin, WU Jianlong, ZHANG Bingxin, et al DOI: 10.3969/j.issn.1006-6233.2025.11.027 Objective: To investigate the correlation between the expression levels of SP1, UBE2C in esophageal squ cell carcinoma (ESCC) tissues and the clinical pathological parameters of patients and to evaluate its predictive value for prognosis. Methods: A total of 127 ES patients who underwent surgical treatment in Central Hospital of Chengde from January 2019 to January 2022 were selected, along with 65 cases of squamous epithelium tissues adjacent to the tumor and 58 cases of esophageal squamous epithelial papilloma tissues. The expression levels of SP1 andBE2C in the observation group and the control group were determined by immunohistochemistry (Envision method) and real-time quantitative PCR (RT-PCR), the expression of the two in ESCC tissues, squamous epithelial papilloma and normal esophageal mucosa tissues adjacent to the tumor was observed, and its correlation with the clinical pathological characteristics was analyzed. Results: The mRNA expression levels of SP1 and UBE2C in the experimental group were higher than those in the control group ( P<0.05), and the results of immunohistochemical staining also showed that the expression levels of SP1 and UBE2C in the experimental were higher than those in the control group, with statistically significant differences (all P<0.05). The expression levels of SP1 and UBE2C inCC tissues were not related to the patient's gender and age (P>0.05), but closely related to the history of smoking, drinking, differentiation degree TNM staging, and lymph node metastasis (P<0.05). Conclusion: Positive expression of SP1 and UBE2C may be related to the and development of ESCC, therefore, SP1 and UBE2C may be used as new potential therapeutic targets, providing new directions for individualized treatment of ESCC patients. 2025 Vol. 31 (11): 1919-1923 [Abstract] ( 136 ) HTML (1 KB)  PDF (1509 KB)  ( 4 ) 1924 The Application Effect of Tianmai Xiaoke Tablets Combined with Empagliflozin in Patients with Diabetes Mellitus Complicated with Heart Failure HAN Rui, ZHANG Yu, et al DOI: 10.3969/j.issn.1006-6233.2025.11.028 Objective: To analyze the application effect of Tianmai Xiaoke Tablets combined with empagliflozin in patients with diabetes mellitus complicated with heart failure. Methods: A total of 102 patients diagnosed with T2DM and heart failure, admitted to Shijiazhuang Maternal and Child Health Hospital and the 980th Hospital of the Joint Logistic Support Force of the People's Liberation Army of China between June 2021 and May 2024, were enrolled and randomly assigned to two groups using a random number generator: an observation group and a control group, with 51 patients in each. The control group underwent standard treatment, whereas the observation group received additional Tianmai Xiaoke Tablet and empagliflozin therapy. Following 12 weeks of treatment, alterations in blood glucose indices, cardiac function parameters, myocardial tissue protein indexes, and quality of life were assessed in both groups, and therapeutic outcomes were compared. Results: Post-treatment, both groups exhibited reductions in the fasting plasma glucose (FPG), 2-hour postprandial plasma glucose (2hPG), and glycated hemoglobin (HbA1c) levels compared to baseline (control group: t / P=5.408/<0.001, 5.567/<0.001, 4.162/<0.001; observation group: t / P=11.63/<0.001, 11.734/<0.001, 9.224/<0.001), with the observation group demonstrating greater decreases across all three indices (t / P=5.575/<0.001, 4.385/<0.001, 3.480/<0.001). Additionally, LVEDD decreased while LVEF increased in both groups post-treatment (control group: t / P=6.231/<0.001 for LVEDD reduction, t / P=2.548/0.014 for LVEF increase; observation group: t / P=7.783/<0.001 for left ventricular end-diastolic diameter (LVEDD) reduction, t / P=5.808/<0.001 for left ventricular ejection Fraction (LVEF) increase), with the observation group showing more pronounced changes (t / P=5.493/<0.001, 2.585/0.011). After treatment, the levels of silent information regulator 2 homologous protein 1 (SIRT1) and uncoupled protein 2 (UCP2) in the control group had no significant changes (t / P=0.141/0.888, 0.445/0.657), while the levels of SIRT1 in the observation group increased significantly (t / P=-4.944/<0.001), and the levels of UCP2 decreased significantly (t / P=17.841/<0.001), both of which were higher than those in the control group (t / P=-5.687/<0.001, 14.305/<0.001). Furthermore, SF-36 scale scores improved across all dimensions in both groups after treatment (control group: t=9.987, 9.259, 8.465, 12.371, 9.769, 9.699, 11.276, 8.964, P<0.001 for all; Observation group: t =19.307, 22.258, 12.985, 19.43, 21.002, 17.785, 19.74, 16.632, all P<0.001), with the observation group scoring higher in every dimension (t =7.959, 11.148, 5.765, 10.055, 7.871, 6.789, 12.113, 7.468, all P<0.001). Ultimately, the observation group exhibited a higher overall treatment efficacy compared to the control group (P<0.05). Conclusion: Using a combination of Tianmai Xiaoke Tablet and Enggligin for treating diabetes patients with heart failure results in more effective blood sugar regulation, enhancement of cardiac function, betterment of quality of life, and improved therapeutic outcomes. 2025 Vol. 31 (11): 1924-1929 [Abstract] ( 160 ) HTML (1 KB)  PDF (1186 KB)  ( 9 ) 1930 Clinical Efficacy Analysis of Neoadjuvant Immunotherapy Combined with Chemotherapy in Patients with Locally Advanced Esophageal Squamous Cell Carcinoma Undergoing Surgery JIANG Feie, LIU Qian, XU Shuonan, et al DOI: 10.3969/j.issn.1006-6233.2025.11.029 Objective: To study the efficacy and safety profiles of neoadjuvant chemoradiotherapy (nCRT) and neoadjuvant immunochemotherapy (nCIT) among patients diagnosed with locally advanced esophageal squamous cell carcinoma (ESCC) and their impact on postoperative recovery. Methods: A total of 165 patients with locally advanced ESCC admitted to The Second Affiliated Hospital of Air Force Medical University from January 2021 to December 2023 were retrospectively analyzed. In line with the neoadjuvant treatment plan, the patients were separated into the control group (administered nCRT, with a sample size of 62) and the study group (receiving nCIT, with 103 participants). The postoperative pathological remission rate, incidence of adverse events, changes in immune function, perioperative indicators and incidence of postoperative complications were compared between the two groups. Results: The R0 resection rate, pathological complete response (pCR) rate, and major pathological response (MPR) rate did not show significant differences between the two groups (P>0.05). However, the occurrence rate of radiation esophagitis and thrombocytopenia in the study group was remarkably reduced compared with the control group (P<0.05). Regarding immune function, the percentages of CD8+ T cells and NK cells, along with the levels of IL-2, IFN-γ and TNF-α, were elevated in the study group relative to the control group (P<0.05), and the CD4, Shaanxi/CD8, Shaanxi ratio was decreased compared to the control group (P<0.05). Compared with the control group, the operation time, postoperative ICU stay time, postoperative chest drainage volume, postoperative drainage tube indwelling time and postoperative hospital stay were all shorter in the study group. Additionally, the total quantity of lymph nodes dissected was higher in the study group than in the control group (P<0.05). The overall occurrence rate of postoperative complications in the study group was lower than that in the control group (P<0.05). Conclusion: nCIT has advantages in reducing radiotherapy-related toxicity, improving postoperative recovery and reducing complications, and may be an optimal regimen for patients with poor tolerance to radiotherapy or those who need rapid recovery after surgery. 2025 Vol. 31 (11): 1930-1936 [Abstract] ( 160 ) HTML (1 KB)  PDF (1138 KB)  ( 10 )

Copyright © Editorial Board of HeBei Med Supported by:Beijing Magtech