龙胆苦苷调节RhoA/ROCK1信号通路对食管鳞状细胞癌的增殖迁移和侵袭的影响

doi:10.3969/j.issn.1006-6233.2025.11.09

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摘要 目的: 探讨龙胆苦苷调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋蛋白激酶1(ROCK1)信号通路对食管鳞状细胞癌增殖、迁移和侵袭的影响。方法: 将人食管鳞状细胞癌细胞KYSE-180分为对照组、L-龙胆苦苷组、M-龙胆苦苷组、H-龙胆苦苷组、H-龙胆苦苷+Narciclasine组。集落形成测定实验和CCK-8检测细胞增殖,划痕愈合实验检测迁移,Transwell实验检测迁移和侵袭,Western blot检测KYSE-180中RhoA和ROCK蛋白表达。结果: 与对照组相比,L-龙胆苦苷组、M-龙胆苦苷组、H-龙胆苦苷组KYSE-180细胞OD₄₅₀值和划痕愈合率降低,集落形成数、迁移和侵袭细胞数减少,RhoA和ROCK1的蛋白表达降低(P<0.05),且呈剂量依赖性效应(P<0.05);与H-龙胆苦苷组比较,H-龙胆苦苷+Narciclasine组KYSE-180细胞的OD₄₅₀值和划痕愈合率提高,集落形成数、迁移和侵袭细胞数增加,RhoA和ROCK1的蛋白表达提高(P<0.05)。结论: 龙胆苦苷抑制RhoA/ROCK1信号通路抑制食管鳞状细胞癌的增殖、迁移和侵袭。

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关键词 ：食管鳞状细胞癌, 龙胆苦苷, RhoA/ROCK1, 增殖, 迁移, 侵袭

Abstract：Objective: To explore the effects of gentiopicroside on the proliferation, migration, and invasion of esophageal squamous cell carcinoma by regulating Ras homolog family member A (RhoA)/rho A-associated coiled-coil kinase (ROCK1) signaling pathway. Methods: Human esophageal squamous cell carcinoma KYSE-180 cells were assigned into untreated group, L-gentiopicroside group, M-gentiopicroside group, H-gentiopicroside group, and H-gentiopicroside+N-arcilasine group. Colony formation assay and CCK-8 were performed to detect cell proliferation. The scratch healing experiment was performed to measure migration. Transwell experiment was performed to measure migration and invasion. Western blot was used to measure the RhoA and ROCK proteins in KYSE-180. Results: Compared with the untreated group, the L-gentiopicroside group, M-gentiopicroside group, and H-gentiopicroside group showed a decrease in OD₄₅₀ value, scratch healing rate of KYSE-180 cells, the numbers of colony formation, migration, and invasive cells, and the RhoA and ROCK1 proteins (P<0.05), and showed a dose-dependent effect (P<0.05). Compared with the H-gentiopicroside group, the H-gentiopicroside+N-arcilasine group showed an increase in OD₄₅₀ value, scratch healing rate of KYSE-180 cells, the numbers of colony formation, migration, and invasive cells, and the RhoA and ROCK1 proteins (P<0.05). Conclusion: Gentiopicroside inhibits the RhoA/ROCK1 signaling pathway and suppresses the proliferation, migration, and invasion of esophageal squamous cell carcinoma.

Key words： Esophageal squamous cell carcinoma Gentiopicroside RhoA/ROCK1 Proliferation Migration Invasion

基金资助:四川省自然科学基金项目,(编号:2025ZNSFSC1864)

通讯作者: 袁跃西

引用本文:

言成一, 彭建明, 顾一敏, 袁跃西. 龙胆苦苷调节RhoA/ROCK1信号通路对食管鳞状细胞癌的增殖迁移和侵袭的影响[J]. 河北医学, 2025, 31(11): 1815-1820.
YAN Chengyi, PENG Jianming, et al. Effects of Gentiopicroside on the Proliferation Migration and Invasion of Esophageal Squamous Cell Carcinoma by Regulating RhoA/ROCK1 Signaling Pathway. HeBei Med, 2025, 31(11): 1815-1820.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.11.09 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I11/1815

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