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2026 Vol. 32, No. 1
Published: 2026-01-31
 
1 The Effect of LncRNA SNHG14 on High Glucose Induced Proliferation and Fibrosis of Glomerular Mesangial Cells by Regulating the miR-101-3p/SGK1 Axis
NIU Xiaojing, YE Hanlu, ZHANG Lifang, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.01
Objective: To explore the effect of LncRNA SNHG14 on high glucose (HG)-induced proliferation and fibrosis of glomerular mesangial cells (GMC) by regulating the miR-101-3p/SGK1 axis. Methods: The serum of patients with diabetic nephropathy (DN) (DN group) and healthy people (NC group) were collected. RT-qPCR was used to detect the expression of SNHG14, miR-101-3p, and SGK1 mRNA in serum. Human GMC (HGMC) was cultured and assigned into NG group, HG group, HG+SNHG14 knockdown control (si-NC) group, HG+SNHG14 knockdown (si-SNHG14) group, HG+si-SNHG14+miR-101-3p inhibitor (in-miR-101-3p) group, and HG+si-SNHG14+SGK1 overexpression plasmid (pcDNA-SGK1) group. RT-qPCR was used to detect the expression of SNHG14, miR-101-3p, and SGK1 mRNA in each group. CCK-8, cloning formation, immunofluorescence, and Western blot were used to detect HGMC proliferation and fibrosis in each group. The dual luciferase and RIP assays were used to detect the relationship between miR-101-3p and SNHG14 (or SGK1). Results: The expression levels of SNHG14 and SGK1 mRNA in serum of patients in DN group were higher than those in the NC group, while the expression of miR-101-3p was lower (P<0.05). Compared with the NG group, SNHG14 expression in the HG group was upregulated, accompanied by significantly increased cell proliferation activity (OD value, colony formation, Ki67-positive cell rate) and elevated expression of fibrosis markers (SGK1, α-SMA, FN, Col IV), while miR-101-3p expression was downregulated (P<0.05). Importantly, compared with the HG+si-NC group, knockdown of SNHG14 (HG+si-SNHG14 group) effectively reversed these HG-induced effects, significantly inhibiting cell proliferation and fibrosis (P<0.05). Furthermore, on the basis of SNHG14 knockdown, either inhibition of miR-101-3p (HG+si-SNHG14+in-miR-101-3p group) or overexpression of SGK1 (HG+si-SNHG14+pcDNA-SGK1 group) could significantly reverse the anti-proliferative and anti-fibrotic effects caused by SNHG14 knockdown (P<0.05). SNHG14 targeted and regulated the miR-101-3p/SGK1 axis(P<0.05). Conclusion: LncRNA SNHG14 promotes HG induced proliferation and fibrosis of GMC by inhibiting miR-101-3p and upregulating SGK1.
2026 Vol. 32 (1): 1-8 [Abstract] ( 42 ) HTML (1 KB)  PDF (1564 KB)  ( 33 )
8 Effects of Isoleucine on the Proliferation Migration Invasion and Apoptosis of Colorectal Cancer Cells by Modulating the NF- κB/AP-1 Signaling Pathway
XIAO Jingjing, JIANG Li, LIU Wei, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.02
Objective: To investigate the impacts of isoliensinine (ISO) on the proliferation, migration, invasion, and apoptosis of colorectal cancer (CRC) cells by modulating nuclear factor-κB (NF- κB)/activator protein 1 (AP-1) signaling pathway. Methods: Caco2 cells were assigned into Control group (normal culture), L-ISO group, M-ISO group, and H-ISO group (added with 10, 20, and 40μmol/L ISO, respectively); ISO+PMA group [40μmol/L ISO+1μmol/L NF-κB signaling pathway activator phorbol ester (PMA)] randomly. EdU staining was used to measure Caco2 cell proliferation. Scratch assay was applied to measure Caco2 cell migration. Transwell experiment was used to detect Caco2 cell invasion. Flow cytometry was applied to measure apoptosis of Caco2 cells. WB was used to test NF-κB and AP-1 proteins in various groups. Results: The L-ISO, M-ISO, and H-ISO groups had lower EdU positive cell rate, scratch healing rate, invasion number, NF-κB, and AP-1 proteins, and higher apoptosis rate than Control group (P<0.05). The ISO+PMA group had higher EdU positive cell rate, scratch healing rate, invasion number, NF- κB, and AP-1 proteins, and lower apoptosis rate than H-ISO group (P<0.05). Conclusion: ISO can inhibit the NF-κB/AP-1 signaling pathway, thereby suppressing the proliferation, migration, and invasion of CRC cells and promoting cell apoptosis.
2026 Vol. 32 (1): 8-13 [Abstract] ( 35 ) HTML (1 KB)  PDF (2614 KB)  ( 33 )
13 The Effects of Esketamine on Depressive-Like Behaviour and Microglial Activation in Rats with Depression via the TLR4/MyD88/NF-κB Pathway
LIU Xuan, ZHANG Xiaomin, LIU Jinting, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.03
Objective: To explore the effects of esketamine (ESK) on depressive-like behaviors and activation of microglia in rats with depression by regulating the Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) pathway. Methods: The rats with depression were constructed, and successfully modeled rats were separated into model group, L-ESK group, H-ESK group (intraperitoneal injection of 10 and 20mg/kg ESK), and ESK+RS09 group (intraperitoneal injection of 20mg/kg ESK+25μg/kg RS09), with 10 rats in each group. Another 10 normally fed rats were marked as the control group. The model group and control group were intraperitoneally injected with an equal amount of physiological saline, once a day for three consecutive weeks. After the administration, rats in each group was weighed, subjected to forced swimming test, sucrose preference test, and open field test. ELISA was used to measure the TNF-α, IL-6, and IL-10 in rat serum. HE staining was used to measure pathological changes in rat hippocampal tissue. Immunohistochemistry was applied to measure the Iba-1, CD206, and iNOS in hippocampal tissues. Moreover, Western blot was applied to detect the TLR4, MyD88, and NF-κB proteins in rat hippocampal tissues. Results: Compared with the control group, the arrangement of hippocampal neurons in the model group was disordered and loose, with phenomena such as vacuoles and nuclear degeneration and transformation observed. The levels of TNF-α, IL-6, Iba-1, iNOS, TLR4, MyD88, and NF-κB were elevated, while the body weight, sucrose preference, number of crawling steps, standing times, IL-10, and CD206 were decreased (P<0.05). Compared with the model group, the damage of hippocampal tissues in the L-ESK and H-ESK groups was alleviated successively. The levels of inactive time, TNF-α, IL-6, Iba-1, iNOS, TLR4, MyD88, and NF-κB were decreased, while the body weight, sucrose preference, number of crawling steps, standing times, IL-10, and CD206 were increased (P<0.05). Compared with the H-ESK group, the levels of inactive time, TNF-α, IL-6, Iba-1, iNOS, TLR4, MyD88, and NF-κB were increased in the ESK + RS09 group, while the body weight, sucrose preference, number of crawling steps, standing times, IL-10, and CD206 were decreased (P<0.05). Conclusion: ESK may inhibit the activation of microglia in rats with depression and improve depressive-like behaviors by suppressing the TLR4/MyD88/NF-κB pathway.
2026 Vol. 32 (1): 13-19 [Abstract] ( 28 ) HTML (1 KB)  PDF (1793 KB)  ( 11 )
20 The Effect of Luteolin on Gastric Mucosal Injury in Rats with Chronic Atrophic Gastritis via Regulation of the AMPK/ULK1 Pathway
YUE Huaqiang, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.04
Objective: To explore the effect of luteolin on gastric mucosal damage in rats with chronic atrophic gastritis by modulating the AMP-activated protein kinase (AMPK)/Unc51-like kinase 1 (ULK1) pathway. Methods: A chronic atrophic gastritis rat model was established and separated into a model group, a luteolin group (80mg/kg), an inhibitor group (20mg/kg AMPK inhibitor Compound C), and a luteolin+activator group (80mg/kg luteolin and 200mg/kg AMPK activator AICAR). The control group consisted of unmodeled rats. Each group consisted of 12 samples, receiving continuous gavage administration for 12 weeks. The spectrophotometer was used to measure gastric mucosal blood flow. ELISA method was used to measure serum gastrin (GAS), plasma motilin (MTL), and levels of IL-1β, TNF-α, SOD, and MDA in gastric mucosal tissue. HE staining was used to observe the pathological morphology of gastric mucosal tissue. TUNEL staining method was used to determine the apoptosis of gastric mucosal tissue cells. Transmission electron microscopy was used to observe the ultrastructure of gastric mucosa. Immunohistochemistry was used to measure the Bcl-2 protein in gastric mucosal tissue. In addition, Western blot was used to measure the AMPK/ULK1 pathway related proteins in gastric mucosal tissue. Results: Compared with the control group, the epithelial cells of the gastric mucosal tissue in the model group shed, and there was more infiltration of inflammatory cells. The shape of the gastric mucosal wall cells was irregular, and the main cell structure was severely injured. The gastric mucosal blood flow was reduced, and the levels of GAS, MTL, SOD, and the expression of Bcl-2 protein declined. While the pathological inflammatory score, apoptosis rate, levels of IL-1β, TNF-α, MDA, and expression of p-AMPK and p-ULK1 proteins increased (P<0.05). Compared with the model group, the gastric mucosal tissue pathological damage was prominently improved in the luteolin group and inhibitor group. The gastric mucosal blood flow, the levels of GAS, MTL, SOD, and the expression of Bcl-2 protein increased. While the pathological inflammatory score, apoptosis rate, levels of IL-1β, TNF-α, MDA, and expression of p-AMPK and p-ULK1 proteins declined (P<0.05). Compared with the luteolin group, the improvement effect of magnolol+activator group on the above indicators in rats can be weakened by AMPK activator (P<0.05). Conclusion: Luteolin may alleviate gastric mucosal injury in rats with chronic atrophic gastritis by inhibiting AMPK/ULK1 pathway.
2026 Vol. 32 (1): 20-27 [Abstract] ( 35 ) HTML (1 KB)  PDF (1879 KB)  ( 30 )
28 An Investigation into the Effects of Hedyotis diffusa on Rats with Liver Cirrhosis Based on the Theory of Qi and Blood Differentiation and Its Influence on NF-κB Expression and Fibrosis
LIN Yan, SHI Guoqin, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.05
Objective: To investigate the effects of Hedyotis diffusa on hepatic fibrosis and NF-κB expression in rats with liver cirrhosis based on the theory of Qi and Blood Differentiation. Methods: The SD rats were randomly divided into a control group, a model group, a positive drug Yishanfu group, and Hedyotis diffusa low- and high-dose groups, with 7 rats in each group. Intraperitoneal injection of carbon tetrachloride was conducted to construct a rat model of liver cirrhosis. HE staining was used to detect pathological changes in liver tissue. ELISA assay was used to detect the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), IL-6, and TNF-α in serum. Masson staining was used to detect the degree of fibrosis in liver tissue. RT-qPCR was used to detect the mRNA expressions of α-SMA, Col1a1, and TGF-β1 in liver tissues. An immunohistochemical staining assay was used to detect the expression and localization of p65 NF-κB protein in liver tissue. Results: Compared with the control group, rats in the model group exhibited marked pathological changes indicative of liver cirrhosis in their hepatic tissue. Serum levels of ALT, AST, TBIL, IL-6, and TNF-α were significantly elevated (P<0.05). Collagen fibre proliferation in the liver tissue was markedly increased, alongside elevated expression of α-SMA, Col1a1, TGF-β1, and p65 NF-κB in the hepatic tissue (P<0.05). Compared with the model group, pathological damage in liver tissue was improved in rats from the positive drug group and the low- and high-dose Hedyotis diffusa groups. Serum levels of ALT, AST, TBIL, IL-6, and TNF-α decreased (P<0.05), collagen fibre proliferation in liver tissue diminished, and liver tissue expression of α-SMA, Col1a1, TGF-β1, and p65 NF-κB in liver tissue decreased (P<0.05). Conclusion: Hedyotis diffusa reduces fibrosis in the liver tissue of rats with cirrhosis by inhibiting inflammation, and the mechanism may be related to the inhibition of NF-κB signaling.
2026 Vol. 32 (1): 28-33 [Abstract] ( 23 ) HTML (1 KB)  PDF (1994 KB)  ( 6 )
33 Effects and Mechanisms of Shengmai Injection on Acute Lung Injury in Rats with Septic Shock Based on the NF-κB/NLRP3 Signaling Pathway
XIE Huanqiong, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.06
Objective: To explore the effects and mechanisms of Shengmai injection on acute lung injury in rats with septic shock based on the NF-κB/NLRP3 signaling pathway. Methods: Male Sprague-Dawley (SD) rats were randomly divided into the control group,the model group,the positive drug dexamethasone group and the Shengmai injection group, with 10 rats in each group. A rat model of septic shock was constructed by intravenous injection of lipopolysaccharide. The serum endotoxinlevel of in rats in each group were detected. The lung coefficient and wet/dry weight ratio of rats in each group were measured. Hematoxylin-eosin (HE) staining was used to detect pathological damage in lung tissue. TUNEL staining was used to detect apoptosis in lung tissues.The protein expression of Bcl-2, Bax, cleaved caspase-3, p-p65 NF-κB/p65 NF-κB, NLRP3, cleaved Caspase-1 (caspase-1 p20) and GSDMD was detected by Western blot. The levels of IL-1β, IL-6, TNF-α in bronchoalveolar lavage fluid were detected by Enzyme-linked immunosorbent(ELISA). Results: Compared with the control group, the serum endotoxin levels of rats in the model group were increased (P<0.05),lung coefficient and wet/dry weight ratio of lung tissue increased(P<0.05),and lung tissue showed significant pathological damage,the apoptosis rate of lung histiocytes increased (P<0.05),the Bcl-2 in lung tissue decreased,while Bax, cleaved caspase-3, p-p65 NF-κB/p65 NF-κB, NLRP3, caspase-1 p20, GSDMD were increased (P<0.05),the levels of IL-1β, IL-6, TNF-α in bronchoalveolar lavage fluid increased (P<0.05). Compared with the model group, the serum endotoxin levels of the positive drug group and Shengmai injection group were decreased (P<0.05),lung coefficient and wet/dry weight ratio of lung tissue decreased (P<0.05), and pathological damage in lung tissue reduced,the apoptosis rate of lung histiocytes decreased (P<0.05),the Bcl-2 in lung tissue increased,while Bax, cleaved caspase-3, p-p65 NF-κB/p65 NF-κB, NLRP3, caspase-1 p20, GSDMD were decreased (P<0.05),the levels of IL-1β, IL-6, TNF-α in bronchoalveolar lavage fluid decreased (P<0.05). Conclusion: Shengmai injection can alleviate lung tissue damage in rats with septic shock by inhibiting lung cell apoptosis and lung inflammation, the mechanism of which may be related to the inhibition of NF-κB/NLRP3 signaling pathway.
2026 Vol. 32 (1): 33-39 [Abstract] ( 25 ) HTML (1 KB)  PDF (1676 KB)  ( 21 )
40 Mechanism Study on the Involvement of Transmembrane BAX Inhibitor Motif 1 in Atrial Fibrillation
GU Lishuang, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.07
Objective: To explore the mechanism of the involvement of Transmembrane BAX Inhibitor Motif 1(TMBIM1) in atrial fibrillation (AF). Methods: Forty adult male Sprague-Dawley rats were randomly divided into five groups: control group, AF group, AF+Vector group, AF+TMBIM1 group and AF+TMBIM1+MMP9 agonist (GC) group, with 8 rats in each group. Except the control group, rats in the other groups were injected with acetylcholine (ACh) and calcium chloride (CaCl2) via the tail vein to an induceatrial fibrillation (AF) model. On the 21st day, the left atrial diameter was analyzed by echocardiography, and the induction rate and duration of atrial fibrillation were evaluated via in vivo electrophysiology. The expressions of α -smooth muscle actin (α-SMA) and matrix metalloproteinase 9(MMP9) in atrial tissue were analyzed by Western blot. Rat cardiomyocytes (H9C2) were divided into sh-NC group, sh-TMBIM1 group, carrier group and TMBIM1 group. Among them, the sh-TMBIM1 group and TMBIM1 group established H9C2 with stable TMBIM1 knockdown or upregulation using short hairpin RNA (shRNA) interference or adenovirus AAV9-ANF-TMBIM1 infection. The fluorescence intensities of MMP9, α-SMA and TMBIM1 in H9C2 cells were analyzed by immunofluorescence. Results: Compared with the control group, AF susceptibility, AF duration and atrial fibrosis area of rats in AF group and AF+Vector group were significantly increased (P<0.05). Compared with AF+Vector group, AF susceptibility, AF duration and atrial fibrosis area of rats in AF+TMBIM1 group were significantly decreased (P<0.05). The left atrial diameter and the expression of MMP9 and α-SMA in atrial tissue of rats in AF group and AF+Vector group were significantly higher than those in control group (P<0.05). Compared with the AF+Vector group, the left atrial diameter and the expression of MMP9 and α-SMA in the atrial tissue of rats in the AF+TMBIM1 group were significantly decreased (P<0.05). Compared with AF+TMBIM1 group, AF susceptibility, AF duration, left atrial diameter, atrial fibrosis area and α-SMA expression in atrial tissues of rats in AF+TMBIM1+GC group were significantly increased (P<0.05). Compared with sh-NC group, the fluorescence intensity of TMBIM1 in H9C2 cells in sh-TMBIM1 group was significantly decreased (P<0.05), while the fluorescence intensities of MMP9 and α-SMA were significantly increased (P<0.05). Compared with Vector group, the fluorescence intensity of TMBIM1 in H9C2 cells in TMBIM1 group was significantly increased (P<0.05), while the fluorescence intensities of MMP9 and α-SMA were significantly decreased (P<0.05). Conclusion: In the rat model of AF induced by Ach-CaCl2, TMBIM1 down-regulation can promote atrial remodeling and AF progression through atrial fibrosis mediated by MMP9 signaling pathway.
2026 Vol. 32 (1): 40-46 [Abstract] ( 24 ) HTML (1 KB)  PDF (2032 KB)  ( 6 )
46 Silent LncRNA NORAD Inhibits Doxetaxel Resistance in Castration-Resistant Prostate Cancer Cells by Enhancing Autophagy via TRIP13 Suppression
MA Qi, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.08
Objective: To investigate the effects and molecular mechanisms of long non-coding RNA (LncRNA) DNA damage activated (NORAD) on docetaxel resistance in castration-resistant prostate cancer (CRPC) cells by regulating thyroid hormone receptor interactor 13 (TRIP13) and the autophagy pathway. Methods: The sensitivity of CRPC cell line PC3 and its docetaxel-resistant strain PC3-DR to docetaxel was compared. PC3-DR cells were divided into 8 groups, including Control group, NORAD silenced group, NORAD silenced negative control group, TRIP13 silenced group, TRIP13 silenced negative control group, NORAD silenced + TRIP13 overexpression group, NORAD silenced + TRIP13 overexpression negative control group, and NORAD silenced + 3-MA (autophagy inhibitor) group. EdU assay was used to detect proliferation, flow cytometry was used to detect apoptosis, scratch assay and Transwell assay were used to analyze migration and invasion. qRT-PCR was used to detect the expression of LncRNA NORAD. Western blot was used to detect the expression of TRIP13, Beclin1, LC3-II/I, and p62 proteins. Each group of PC3-DR cells was treated with docetaxel, and EdU assay and flow cytometry were used to detect proliferation and apoptosis, respectively, to analyze the changes in docetaxel sensitivity. Results: Compared with the PC3 group, the PC3-DR group showed significantly reduced inhibition rates of proliferation, migration, and invasion, and reduced apoptosis promotion rate after docetaxel treatment (P<0.05). Compared with the Control group and NORAD silenced negative control group, the NORAD silenced group showed significantly reduced cell proliferation, migration, and invasion (P<0.05), with significantly downregulated expression of LncRNA NORAD and TRIP13, significantly upregulated LC3-II/I ratio and Beclin1, and significantly downregulated p62 (all P<0.05). Compared with the Control group or NORAD silenced negative control group, the TRIP13 silenced group showed significantly downregulated expression of TRIP13, significantly upregulated LC3-II/I ratio and Beclin1, and significantly downregulated p62 (all P<0.05). Overexpression of TRIP13 or 3-MA treatment could partially reverse the effects of NORAD silencing (all P<0.05). Additionally, the proliferation inhibition rate and apoptosis promotion rate of the NORAD silenced group or TRIP13 silenced group were significantly increased compared with the Control group (all P<0.05), and overexpression of TRIP13 or 3-MA treatment could partially reverse the increased effects of NORAD silencing on proliferation inhibition rate and apoptosis promotion rate (all P<0.05). Conclusion: Silencing LncRNA NORAD promotes the sensitivity of PC3-DR cells to docetaxel by inhibiting TRIP13 expression and activating the autophagy pathway, thereby reversing docetaxel resistance in CRPC cells. This provides a theoretical basis for targeting the LncRNA NORAD/TRIP13/autophagy axis in the treatment of drug-resistant prostate cancer.
2026 Vol. 32 (1): 46-55 [Abstract] ( 25 ) HTML (1 KB)  PDF (3943 KB)  ( 14 )
55 Analysis of the Correlation Between Serum 14-3-3β Protein and CD5L Levels with the Risk of Poor Prognosis in Adult Patients with Bronchial Asthma Complicated by Pulmonary Infection
ZHENG Xiangzhen, CHEN Wendeng, YU Le, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.09
Objective: To investigate the correlation between serum 14-3-3β protein, CD5 antigen-like protein (CD5L), and the risk of poor prognosis in adult patients with bronchial asthma complicated by pulmonary infection. Methods: A total of 155 adult patients with bronchial asthma complicated by pulmonary infection in 909th Hospital from January 2022 to January 2024 were selected. All patients received standardized treatment for bronchial asthma and individualized anti-infective therapy. They were followed up for one year and categorized into a good prognosis group and a poor prognosis group based on their prognosis. The clinical data, serum 14-3-3β protein, and CD5L levels were compared between the two groups. Logistic regression analysis was used to identify the influencing factors of poor prognosis in adult patients with bronchial asthma complicated by pulmonary infection. Smooth curve fitting analysis was employed to explore the relationship between serum 14-3-3β protein, CD5L, and the risk of poor prognosis. Receiver Operating Characteristic (ROC) curves and Area Under the Curve (AUC) were used to assess the predictive value of serum 14-3-3β protein and CD5L for poor prognosis risk. The interaction index (γ) was used to analyze the interactive effect of serum 14-3-3β protein and CD5L on poor prognosis risk. Results: At a 1-year follow-up, 4 cases were lost to follow-up, 104 cases had a good prognosis, and 47 cases had a poor prognosis. The serum level of 14-3-3β protein was higher in the poor prognosis group than in the good prognosis group, while the level of CD5L was lower (P<0.05). Logistic regression analysis indicated that serum 14-3-3β protein and CD5L levels were risk factors for poor prognosis in adult patients with bronchial asthma complicated by pulmonary infection (P<0.05). Smooth curve fitting analysis showed that after adjusting for other factors, serum 14-3-3β protein level exhibited a monotonically positive correlation with the risk of poor prognosis in adult patients with bronchial asthma complicated with pulmonary infection (P<0.05), and serum CD5 L level showed a monotonically negative correlation with the risk of poor prognosis in adult patients with bronchial asthma complicated with pulmonary infection (P<0.05). The AUC values for predicting poor prognosis in adult patients with bronchial asthma complicated by pulmonary infection using serum 14-3-3β protein and CD5L levels were 0.755 and 0.779, respectively. The optimal cutoff values were 57.84 ng/mL and 177.73 pg/mL, with sensitivities of 82.98% and 68.09%, and specificities of 63.46% and 80.77%, respectively. The combined prediction of poor prognosis in adult patients with bronchial asthma complicated by pulmonary infection had an AUC of 0.884, a sensitivity of 76.60%, and a specificity of 87.50%, showing significantly higher predictive value than either individual predictor alone (Z=2.542, 2.129, P=0.011, 0.033). High expression of serum 14-3-3β protein and low expression of CD5L showed a positive interaction in predicting poor prognosis in adult patients with bronchial asthma complicated by pulmonary infection (γ=2.344, P<0.001). Conclusion: Serum 14-3-3β protein and CD5L are independent factors influencing the poor prognosis risk in adult patients with bronchial asthma complicated by pulmonary infection. Furthermore, high expression of serum 14-3-3β protein and low expression of CD5L exhibit a positive interaction, indicating a high combined predictive value.
2026 Vol. 32 (1): 55-62 [Abstract] ( 25 ) HTML (1 KB)  PDF (1351 KB)  ( 12 )
62 Correlation Analysis of Serum PCSK9 LTB4 and ADAM17 Levels with Disease Progression in Patients with Chronic Atrophic Gastritis
YOU Zhaoyang, LI Shibei, LUO Run, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.010
Objective: To investigate the correlation between the levels of serum proprotein convertase subtilase 9 (PCSK9), leukotriene B4 (LTB4), and a disintegrin and metalloproteinase 17(ADAM 17) in patients with chronic atrophic gastritis (CAG) and disease progression, and analyze their predictive value for disease progression. Methods: A prospective study was conducted on 210 patients with CAG from January 2021 to January 2023. Patients were divided into a progression group and a non-progression group based on whether they experienced disease progression within 2 years after treatment. The clinical data, serum levels of PCSK9, LTB4, and ADAM17 were compared between the two groups. Logistic regression analysis and restricted cubic spline (RCS) curve analysis were used to analyze the relationship between serum PCSK9, LTB4, and ADAM17 and disease progression, and stratified analysis was conducted. The predictive value of serum PCSK9, LTB4, and ADAM17 for disease progression was evaluated using the receiver operating characteristic (ROC) curve. Results: The proportion of Hp infection, alcohol consumption history, and spicy diet in the progression group were higher than those in the non-progression group, while the PGⅠ/PGⅡ ratio was lower than that in the non-progression group (P<0.05); the levels of serum PCSK9, LTB4, and ADAM17 in the progression group were (188.73±30.17) ng/mL, (9.52±2.06) pg/mL, and (74.56±12.25) ng/L, respectively, which were significantly higher than those in the non-progression group (160.49±25.64) ng/mL, (7.78±1.51) pg/mL, and (63.17±9.44) ng/L (P<0.05); after excluding other factors such as Hp infection, alcohol consumption history, spicy diet, and PGⅠ/PGⅡ ratio, serum PCSK9, LTB4, and ADAM17 remained independent factors for the progression of CAG (P<0.05); when serum PCSK9 was ≥144.69 ng/mL, LTB4 was ≥7.01 pg/mL, and ADAM17 was ≥67.16 ng/L, there was a significant correlation with the progression of CAG (P<0.05); there was an interactive effect between serum PCSK9, LTB4, ADAM17, Hp infection, and PGⅠ/PGⅡ ratio in the progression of CAG (interaction P<0.05); the AUCs of serum PCSK9, LTB4, ADAM17, and their combination for predicting the progression of CAG were 0.763, 0.755, 0.780, and 0.918, respectively, and the AUC of the combination was significantly higher than that of the single index (P<0.05). Conclusion: The levels of serum PCSK9, LTB4, and ADAM17 increase in patients with CAG progression, and are significantly correlated with CAG progression. Combined detection of their levels has certain predictive value for CAG progression.
2026 Vol. 32 (1): 62-69 [Abstract] ( 22 ) HTML (1 KB)  PDF (1415 KB)  ( 7 )
69 Analysis of the Relationship and Predictive Value of NLRP3 IL-1Ra and TBP-2 Levels in Gestational Diabetes Mellitus
XIE Changshan, WANG Qin, WANG Yu, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.011
Objective: To investigate the predictive value of NOD-like receptor protein 3 (NLRP3), IL-1 receptor antagonist (IL-1Ra) and thioredoxin binding protein-2 (TBP-2) levels in gestational diabetes mellitus (GDM). Methods: A total of 103 GDM patients admitted to our hospital between November 2021 and December 2024 were selected as the GDM group while 52 healthy pregnant patients were chosen as the control group. The levels of NLRP3, IL-1Ra, TBP-2 and other clinical data were compared between the two groups. Binary Logistic regression analysis was used to screen the influencing factors of GDM, and ROC curve was used to verify its predictive performance. Results: Compared with the control group, the GDM group had more macrosomia, and the levels of FPG, TG, LDL-C, NLRP3, IL-1Ra and TBP-2 were increased. (P<0.05). FPG, TG, LDL-CNLRP3, IL-1Ra and TBP-2 were independent risk factors for GDM (P<0.05). ROC curve analysis showed that the area under the curve of single and combined detection of NLRP3, IL-1 Ra and TBP-2 levels in the diagnosis of GDM was 0.639, 0.698, 0.645 and 0.785, respectively, and the combined detection had the highest efficiency. Conclusion: NLRP3, IL-1Ra and TBP-2 are closely related to the occurrence of GDM and have certain predictive value.
2026 Vol. 32 (1): 69-74 [Abstract] ( 17 ) HTML (1 KB)  PDF (1281 KB)  ( 6 )
74 Expression Levels of sTREM-1 HNL and LDH in Serum of Hospital-Acquired Infection Patients and Their Predictive Value for Infection
WANG Kuien, ZHANG Yongna, ZHANG Qingkun, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.012
Objective: To investigate the expression and predictive value of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), neutrophil apolipoprotein (HNL), lactate dehydrogenase (LDH) in serum of patients with acute ischemic stroke (AIS) and hospital infection. Methods: A total of 136 patients with AIS admitted to our hospital between January 2020 and August 2023 were selected and divided into 58 cases in the infected group and 78 cases in the non-infected group according to whether or not hospital infection occurred. Serum LDH levels were detected by colorimetric assay; the expression levels of sTREM-1 and HNL were detected by enzyme-linked immunosorbent assay (ELISA); Pearson correlation analysis was performed to analyze the correlation between serum sTREM-1, HNL, and LDH, and Hs-CRP, WBC, and D-D; Logistic analysis was used to analyze the influencing factors. ROC curve was used to analyze the predictive value. Results: The levels of serum Hs-CRP, WBC, DD, sTREM-1, HNL and LDH in infected group were significantly higher than those in non-infected group (P<0.05); the Pearson's correlation analysis showed that serum sTREM-1, HNL, LDH were positively correlated with Hs-CRP, WBC, and DD (P<0.05); Serum sTREM-1, HNL, LDH, Hs-CRP, WBC, and D-D were all influencing factors in AIS combined with hospital-acquired infections (P<0.05); and ROC curves showed that serum sTREM-1, HNL and LDH jointly predicted AIS combined hospital infection with an AUC of 0.919, which was significantly higher than that of the three tests alone (Z=3.447, 2.332, 2.442, all P<0.05). Conclusion: The expression levels of serum sTREM-1, HNL, and LDH are obviously increased in patients with AIS complicated by hospital infection, and the combination of the three is more effective in predicting AIS complicated by hospital infection.
2026 Vol. 32 (1): 74-79 [Abstract] ( 26 ) HTML (1 KB)  PDF (1259 KB)  ( 4 )
79 Analysis of Pathogenic Bacteria Distribution and Drug Resistance in Children with Mycoplasma Pneumoniae Pneumonia Complicated by Secondary Infections and Clinical Significance of Serum Gas6 and PGE2 Assays
LIU Xiping, WEN Yi
DOI: 10.3969/j.issn.1006-6233.2026.01.013
Objective: To investigate the distribution of pathogens in children with Mycoplasma pneumoniae pneumonia (MPP) complications, to analyze their antimicrobial resistance, and to evaluate the clinical significance of detecting serum levels of growth arrest-specific protein 6 (Gas6) and prostaglandin E2 (PGE2).Methods: A total of 214 children with MPP complicated with bacterial infection admitted from April 2021 to May 2024 were selected as the infection group, while 210 children with simple MPP were selected as the MPP group during the same period. The pathogenic bacteria of children with MPP complicated with bacterial infection were cultured and identified, and were tested for drug resistance. General information of children in two groups was collected. The coagulation method was applied to detect coagulation function indicators. Enzyme linked immunosorbent assay (ELISA) was applied to detect immune function indicators, inflammatory factors, and serum levels of Gas6 and PGE2. Multivariate logistic regression was applied to analyze the influencing factors of concurrent bacterial infections in children with MPP. Receiver operating characteristic curve (ROC) was applied to analyze the diagnostic value of serum Gas6 and PGE2 levels for secondary bacterial infections in children with MPP. Results: A total of 231 strains of pathogenic bacteria were isolated from 214 children with MPP-complicated bacterial infections, with Gram negative bacteria accounting for the highest proportion. Children with MPP-complicated bacterial infections were more resistant to erythromycin, azithromycin, clarithromycin, roxithromycin, and spiramycin. The prothrombin time (PT), activated partial thromboplastin time (APTT), levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA) in the infected group were lower than those in the MPP group, the levels of fibrinogen (FIB), hypersensitive C-reactive protein (hs-CRP), interleukin-6 (IL-6), interleukin-1β (IL-1β), Gas6, and PGE2 were higher than those in the MPP group (P<0.05). The levels of hs-CRP, IL-6, IL-1β, Gas6, and PGE2 were influencing factors in children with MPP with secondary bacterial infections (P<0.05). The combination of serum Gas6 and PGE2 levels for diagnosing secondary bacterial infections in children with MPP had a higher AUC than the serum Gas6 level alone (Z=6.814, P<0.001), and serum PGE2 level alone (Z=7.222, P<0.001). Conclusion: The pathogenic bacteria in children with MPP complicating bacterial infection are mainly gram-negative bacteria, which have high resistance to multiple antibiotics, serum Gas6 and PGE2 levels are elevated. The combination of the two has high diagnostic value for MPP complicating bacterial infection.
2026 Vol. 32 (1): 79-85 [Abstract] ( 19 ) HTML (1 KB)  PDF (1252 KB)  ( 6 )
85 A Comparative Study of the Efficacy of Transfrontal Keyhole Approach Under Neuroendoscopy versus Microsurgical Translateral Fissure-Insular Approach for Treating Basal Ganglia Haemorrhage
LIANG Xiaobo, YUAN Ruizhi, LIU Jianpeng, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.014
Objective: To investigate the effects of transfrontal keyhole approach and microsurgical translateral fissure-insular approach on the treatment of basal ganglia haemorrhage and their impact on the levels of serum heart-type fatty acid-binding protein (h-FABP), aquaporin 4 (AQP4), and astrocyte calcium-binding protein (S100β). Methods: A total of 196 patients with intracerebral hemorrhage in basal ganglia region admitted to Yuncheng Central Hospital Affiliated to Shanxi Medical University from January 2022 to October 2024 were selected and divided into two groups according to different surgical methods, 98 patients in the endoscopic group underwent neuroendoscopic hematoma removal (neuroendoscopic surgery), and 98 patients in the microscopic group underwent microscopic hematoma removal (microscopic surgery). The hematoma clearance effect and surgical indexes of the two groups were observed. The levels of serum perioperative brain injury factors (h-FABP, AQP4, S100β) were detected by enzyme-linked immunosorbent assay. The neurological function [National Institutes of Health Stroke Scale (NIHSS) scores], activities of daily living (ADL), prognosis [Glasgow Outcome Scale (GOS)] before operation, 3 months, and 6 months after operation, and incidence of complications were compared between the two groups. Results: There was no significant difference in the total clearance rate of hematoma between the two groups (P>0.05). The complete clearance rate of the microscope group was 63.27%, which was higher than the endoscopic group at 45.92%, and the difference was statistically significant (χ2= 5.269, P=0.022). The operation time of the endoscopic group was (98.41±12.16) min, which was shorter than the microscopic group at (138.52±18.79) min, and the intraoperative blood loss was (90.27±10.28) mL, less than the microscopic group at (106.43±13.64) mL, the differences were statistically significant (t=17.741, 9.366, all P<0.001); At 1 d and 3 d after operation, the serum h-FABP levels in the endoscopic group were (20.15±3.25)μg/L and (15.41±3.12)μg/L, respectively, lower than the microscopic group at (24.73±4.39)μg/L and (20.03±4.07)μg/L. The levels of AQP4 were (1.32±0.22)ng/L and (0.85±0.17)ng/L, respectively, lower than the microscopic group at (1.61±0.28)ng/L and (1.04±0.21)ng/L. The levels of S100β were (1.25±0.27)μg/L and (0.89±0.23)μg/L, respectively, lower than the microscope group at (1.54±0.35)μg/L and (0.98±0.26)μg/L. The differences were statistically significant (t=8.301, 8.918, 8.062, 6.962, 6.495, 4.278, all P<0.001). At 6 months after operation, the NIHSS score of the microscope group was (6.11±0.83), lower than the endoscopic group at (7.02±0.91), and the ADL score was (81.12±3.62), higher than the endoscopic group at (77.29±3.90), the differences were statistically significant (t=7.314, 7.125, all P<0.001). At 6 months after operation, the prognosis of the microscopic group was better than that of the endoscopic group, and the difference was statistically significant (u=2.001, P=0.045). The incidence of complications in the endoscopic group was 4.08%, lower than the microscopic group at 12.24%, the difference was statistically significant (χ2=4.356, P=0.037). Conclusion: Neuroendoscopic surgery and microscope surgery each have their advantages and disadvantages in the treatment of basal ganglia haemorrhage. Neuroendoscopic surgery can optimize the surgical process and reduce early brain damage, while microscope surgery can improve the complete removal rate of hematoma, achieve better effects in terms of long-term neurological function and prognosis improvement in patients, and offer higher safety. Clinically, the surgical method should be selected based on the actual situation of the patient.
2026 Vol. 32 (1): 85-92 [Abstract] ( 26 ) HTML (1 KB)  PDF (1906 KB)  ( 5 )
92 Comparison of the Efficacy of TurboHawk Percutaneous Mechanical Plaque Resection and PTA with Stent Implantation in Treating Short-Segment Superficial Femoral Arteriosclerosis Obliterans
LI Zongyu, GUAN Hao, LAI Jingyue, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.015
Objective: To analyze the efficacy of TurboHawk percutaneous mechanical plaque resection and angioplasty (PTA) + stent implantation in the treatment of short-segment superficial femoral arteriosclerosis obliterans (ASO) and its influence on postoperative primary patency rate and recurrence. Methods: A total of 122 patients with short-segment superficial femoral ASO in the hospital from March 2022 to September 2023 were selected and divided into control group (n=61, PTA combined with stent implantation) and observation group (n=61, TurboHawk percutaneous mechanical plaque resection combined with PTA + stent implantation) with the stratified randomization method. The control group was treated with PTA + stent implantation, while the observation group was additionally treated with TurboHawk percutaneous mechanical plaque resection on the basis of the control group. The technical success rate, primary patency rate, hemodynamic parameters [superficial femoral artery blood flow, ankle-brachial index (ABI), pulsatility index (PI), resistance index (RI)], plasma parameters [endothelin 1 (ET1), visceral adipose tissue-derived serine protease inhibitor (Vaspin), fibrinogen (FIB)], visual analogue pain score (VAS), recurrence and postoperative major adverse events (MAE) were observed in both groups. Results: The technical success rates in the groups of patients were both 100% (61/61) (P>0.05). The technical success rate in observation group was significantly higher than that in control group (P<0.05). At 6 months after surgery, the superficial arterial blood flow, ABI and primary patency rate in observation group were higher while PI, RI, Vaspin and FIB were lower than those in control group (P<0.05). The ET1 level in observation group was lower than that in control group at 1 week and 6 months after surgery (P<0.05). VAS in observation group immediately after surgery and before discharge was significantly lower than that in control group (P<0.05). The incidence rate of MAE within 6 months after surgery in observation group was lower than that in control group (P<0.05). Conclusion: The application of TurboHawk percutaneous mechanical plaque resection and PTA + stent implantation has significant efficacy in the treatment of short-segment superficial femoral ASO, and it can enhance the technical success rate and primary patency rate, improve the hemodynamic indicators, accelerate the postoperative pain relief, and prevent MAE and ASO recurrence, and it is conducive to significantly improving the prognosis of patients with short-segment superficial femoral ASO.
2026 Vol. 32 (1): 92-99 [Abstract] ( 20 ) HTML (1 KB)  PDF (1161 KB)  ( 4 )
99 The Predictive Value of Combined SerummiR-424 miR-503 and CA199 for Postoperative Prognosis in Pancreatic Cancer Patients
HAN Qiang, CHENG Ying, ZHAO Yunhong
DOI: 10.3969/j.issn.1006-6233.2026.01.016
Objective: To explore the specific application value of the combined detection of serum miR-424, miR-503 and CA199 in the prognosis assessment of pancreatic cancer. Methods: 80 patients with pancreatic cancer who were admitted to our hospital from March 2022 to March 2024 were selected. The levels of miR-424, miR-503 and CA199 in serum were determined by qRT-PCR. According to the prognosis of the patients, they were divided into the good prognosis group and the poor prognosis group. Logistic regression analysis was used to identify the influencing factors of poor prognosis. The receiver operating characteristic (ROC) curve was used to analyze the predictive value of poor prognosis. Results: The preoperative serum levels of miR-424, miR - 503, and CA199 in the poor prognosis group were significantly higher than those in the good prognosis group(P<0.05). Before the surgery, the levels of serum miR-424, miR-503 and CA199 in the poor prognosis group were significantly higher than those in the good prognosis group (P<0.05). The median survival time of patients with stage III, lymph node metastasis, and low differentiation was significantly shorter than that of patients with stage I-II, no lymph node metastasis, and moderate/high differentiation (P<0.05). The levels of serum miR-424, miR-503 and CA199 before the surgery were all independent risk factors for poor prognosis in pancreatic cancer patients (P<0.05). The AUC of combined detection of serum miR-424, miR-503 and CA199 was significantly higher than that of single indicator detection (P<0.05). Conclusion: The combined detection of serummiR-424, miR-503, andCA199has important clinical significance in the prognosis evaluation of pancreatic cancer. It can provide a basis for individualized treatment and contribute to revealing the molecular mechanism of the occurrence and development of pancreatic cancer. In the future, combining with emerging biomarkers is expected to further expand its application scope.
2026 Vol. 32 (1): 99-104 [Abstract] ( 23 ) HTML (1 KB)  PDF (1289 KB)  ( 5 )
105 Construction and Clinical Significance of a Nomogram Prediction Model for Poor Prognosis Risk in Emergency Patients with ACI Complicated by Severe Enlarged Perivascular Spaces
HAN Mingming, TANG Xiuli, HAN Mi
DOI: 10.3969/j.issn.1006-6233.2026.01.017
Objective: To analyze the influencing factors for the poor prognosis in emergency patients with acute cerebral infarction (ACI) complicated by severe enlarged perivascular spaces (EPVSs), construct a risk nomogram prediction model, and analyze its clinical guiding significance. Methods: A total of 300 emergency patients with severe EPVSs ACI admitted from January 2021 to November 2024 were prospectively selected as the research objects and divided into a modeling set (n=210) and a validation set (n=90) at a ratio of 7∶3. According to the modified Rankin scale scores, the modeling set was further divided into a good prognosis group (0~2 points, n=127) and a poor prognosis group (3~6 points, n=83). The basic admission information, clinical medical history and other general data of ACI patients were collected, and relevant laboratory indicators were detected. Multivariate Logistic regression was used to analyze the influencing factors for the poor prognosis in emergency patients with ACI complicated with severe EPVSs. Then the rms package was applied to construct a nomogram prediction model. The receiver operating characteristic (ROC) curve was used to evaluate the predictive efficacy of the model; the calibration curve was used to evaluate the consistency between the predicted probabilitiy of the model and the observed results; the decision curve was used to evaluate the net benefit of the model. Results: The poor prognosis rates in the modeling set and validation set were 39.52% and 35.56%, respectively. In the modeling set, the proportion of patients aged ≥65 years with infarctions in key cerebral regions and complicated with hypertension and leukoaraiosis, as well as the scores of the National Institutes of Health Stroke Scale (NIHSS),infarct size,the quantity of enlarged perivascular spaces (EPVSs), and levels of high-sensitivity C-reactive protein (hs-CRP) were all significantly higher in the poor prognosis group than those in the good prognosis group(all P<0.05). Age ≥65 years, hypertension (yes), increased NIHSS score, larger infarct size, and greater EPVSs quantity were independent risk factors for poor prognosis in emergency patients with acute cerebral infarction (ACI),complicated with severe EPVSs (P<0.05). A nomogram prediction model was constructed based on the results of multivariate Logistic regression analysis. The area under the ROC curve (AUC) of the modeling set and validation set was 0.810 (95%CI: 0.750~0.860) and 0.802 (95%CI: 0.741~0.853), respectively. The predicted curves in both sets showed good fit with the actual curves. The threshold probabilities in decision curve analysis were all greater than 0. Conclusion: Age, hypertension, NIHSS score, infarct area, and EPVSs quantity are independent influencing factors for the poor prognosis in emergency patients with ACI complicated with severe EPVSs. The nomogram prediction model constructed based on these results has high predictive efficacy which provides an effective reference for clinical prognosis assessment and personalized treatment.
2026 Vol. 32 (1): 105-112 [Abstract] ( 19 ) HTML (1 KB)  PDF (1355 KB)  ( 11 )
112 Analysis of the Influencing Factors and Predictive Value of Stroke Recurrence in High-risk Non-Disabling Ischemic Cerebrovascular Events Patients
DONG Jingjing, XU Hui, TU Feng, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.018
Objective: To investigate the impact and predictive value of serum biomarkers and clinical characteristics on stroke recurrence in patients with high-risk non-disabling ischemic cerebrovascular events (HR-NICE) patients. Methods: A total of 105 patients with high-risk non-disabling ischemic stroke from February 2022 to February 2024 were enrolled and divided into two groups according to recurrence status: recurrence group (24 cases) and non-recurrence group (81 cases). Serum biomarker levels, clinical characteristics and follow-up data were collected. Univariate and multivariate analyses were used to identify factors influencing recurrence and ROC curves were employed to evaluate their predictive value. Results: Among the 105 patients, the recurrence rate was 22.86%. Compared with the non-recurrence group, the proportion of diabetic patients and the levels of hs-CRP, sCD 40L, Lp-PLA2 and Hcy were significantly elevated in the recurrence group(P<0.05). Multivariate Logistic regression analysis showed that hs-CRP [OR (95% CI) = 2.697 (1.410~5.157)], sCD 40L [OR (95% CI) = 15.029 (3.120~72.408)], and Hcy [OR (95% CI) = 1.966 (1.126~3.434)] were independent risk factors for stroke recurrence in HR-NICE patients, with P values all <0.05. The combined ROC curve for hs-CRP, sCD 40L, and Hcy showed an AUC (95% CI) of 0.982 (0.960~1.000), with a sensitivity of 96.3% and a specificity of 91.7%. Conclusion: hs-CRP, sCD 40L and Hcy are risk factors for stroke recurrence in HR-NICE patients. Monitoring these biomarkers provides valuable predictive information for assessing the prognosis of HR-NICE patients.
2026 Vol. 32 (1): 112-116 [Abstract] ( 28 ) HTML (1 KB)  PDF (1247 KB)  ( 6 )
117 Study on the Correlation between m5C RNA Methylation Modification and Vestibular Dysfunction and Age-Related Hearing Loss
Zhou Xu, Si Fengzhi
DOI: 10.3969/j.issn.1006-6233.2026.01.019
Objective: To investigate the correlation of m5C RNA methylation modification in vestibular function and age-related hearing loss. Methods: A total of 24 inbred C57BL/6J mice were used, which were 5 months old (adult mouse group), 12 months old (middle-aged mouse group) and 20 months old (elderly mouse group), 8 mice in each group. The ROS levels in cochlea and vestibular tissues were determined by DCFH-DA (dichlorodihydrofluorescein-acetoacetate) method. Cochlea and vestibular tissues’ mitochondrial DNA integrity was determined by TAMRA probes. Cochlea and vestibular tissues’ m5C levels of GRM7 mRNA were analyzed by m5C RNA methylation immunocoprecipitation. The levels of NSUN2 in cochlea and vestibular tissues were determined by Western blot. Results: Compared with adult mice group and middle-aged mice group, the average ABR thresholds in aged mice group were increased at 8, 16 and 32 kHz (P<0.05). The relative levels of ROS in cochlear tissues and vestibular tissues were increased (P<0.05). The proportion of mtDNA containing CD (%) was decreased (P<0.05). The relative expression levels of NSUN2 were decreased (P<0.05). The relative expression levels of GRM7 Input mRNA were decreased (P<0.05). The relative expression levels of GRM7 m5C mRNA was decreased (P<0.05). In aged mice group, compared with the cochlear tissue group, the relative levels of ROS in vestibular tissue group was lower (P<0.05), and the relative expression levels of GRM7 Input mRNA and GRM7 m5C mRNA were higher (P<0.05). Conclusion: Down-regulation of m5C RNA methylation and decreased expression of NSUN2 were associated with vestibular function and age-related hearing loss.
2026 Vol. 32 (1): 117-122 [Abstract] ( 16 ) HTML (1 KB)  PDF (1225 KB)  ( 12 )
122 Study on the Efficacy Mechanism of Different Doses of Progesterone in Patients with Threatened Abortion in Early Pregnancy Based on the Changes of Serum BPA and PAPP-A Levels
FANG Xiaobai, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.020
Objective: To analyze the effects of different doses of progesterone on serum bisphenol A (BPA) and pregnancy-related plasma protein A (PAPP-A) levels in patients with threatened abortion in early pregnancy. Methods: A total of 96 cases with threatened abortion in early pregnancy admitted to our hospital from July 2023 to July 2024 were selected as the study subjects and assigned to the observation group. The patients were treated with different doses of progesterone, and randomly divided into a low-dose group (Group A), a medium-dose group (Group B) and a high-dose group (Group C), with 32 cases in each group.Randomization was performed using a computer-generated random number table, and the allocation concealment was ensured by a dedicated person managing the grouping scheme. Serum levels of BPA and PAPP-A were measured using the enzyme-linked immunosorbent assay (ELISA) to analyze the pregnancy maintenance outcomes. Based on the outcomes,the cases in the observation group was further classified into an effective pregnancy maintenance group (n=66) and an ineffective pregnancy maintenance group (n=30). The relationship was clarified between serum levels of BPA and PAPP-A and the efficacy of different progesterone doses in pregnancy maintenance treatment for patients with threatened abortion in early pregnancy.Results: After treatment, compared with Group A, the BPA expression levels in Groups B and C decreased, while the PAPP-A expression levels increased (P<0.05). Compared with Group C, the success rates of pregnancy maintenance in Groups A and B were higher (P<0.05). In comparison to the ineffective pregnancy maintenance group, the expression levels of BPA were decreased and those of PAPP-A were increased in the effective pregnancy maintenance group in patients with threatened abortion in early pregnancy (P<0.05). BPA exhibited a negative correlation with the efficacy of low-dose, medium-dose, and high-dose pregnancy maintenance treatments (P<0.05), while PAPP-A showed a positive correlation with the efficacy of these treatments (P<0.05). Logistic regression analysis indicated that BPA and PAPP-A in patients with threatened abortion in early pregnancy were the primary risk factors influencing the efficacy of progesterone-based pregnancy maintenance treament (P<0.05). ROC analysis revealed that the combination of serum BPA and PAPP-A for the efficacy of progesterone-based pregnancy maintenance treatment in patients with threatened abortion in early pregnancy had high predictive value (P<0.05).Conclusion: Pregnancy maintenance treatment with different doses of progesterone in patients with threatened abortion in early pregnancy can significantly improve the pregnancy maintenance rate and reduce the risk of abortion. Among them, the medium-dose progesterone has the best treatment effect, which can significantly improve the serum levels of BPA and PAPP-A, and enhance the pregnancy outcomes of patients.
2026 Vol. 32 (1): 122-128 [Abstract] ( 23 ) HTML (1 KB)  PDF (1248 KB)  ( 9 )
129 Relationship between Peripheral Blood sCD14 IL-35 and Secondary Pulmonary Infection in Patients with Non-small Cell Lung Cancer after Interventional Therapy
GAO Pengfei, HU Jiaqing, SHU Shengguang
DOI: 10.3969/j.issn.1006-6233.2026.01.021
Objective: To investigate the relationship between the levels of soluble cluster of differentiation 14 (sCD14) and interleukin-35 (IL-35) in peripheral blood and secondary pulmonary infection in patients with non-small cell lung cancer (NSCLC) after interventional therapy, and to analyze their predictive value for secondary pulmonary infection. Methods: A total of 298 patients with NSCLC in our hospital from May 2021 to December 2024 were selected and treated with interventional ablation. According to whether there was secondary pulmonary infection within 1 week after operation, they were divided into infection group and non-infection group. The baseline data of the two groups were analyzed by propensity score matching. The levels of sCD14, IL-35, C-reactive protein (CRP) and procalcitonin (PCT) in peripheral blood before and after interventional therapy were compared. The correlation between the levels of sCD14, IL-35 and CRP, PCT was analyzed. The linear relationship between sCD14, IL-35 and secondary pulmonary infection in patients with NSCLC as well as the predictive value of peripheral blood sCD14 and IL-35 for secondary pulmonary infection in patients with NSCLC after interventional therapy were also analyzed. Results: Of 298 NSCLC patients undergoing interventional therapy, 42 cases of secondary pulmonary infection were enrolled in the infection group, and the remaining 256 cases were enrolled in the non-infection group. A total of 64 cases were successfully matched by propensity score matching, including 32 cases in the infection group and 32 cases in the non-infection group. After interventional therapy, the levels of sCD14, CRP and PCT in the infection group were higher than those in the non-infection group, while the level of IL-35 was lower than that in the non-infection group (P<0.05).After NSCLC interventional therapy,the level of sCD14 in peripheral blood of patients with secondary pulmonary infection was positively correlated with CRP and PCT (r=0.665, 0.686, P<0.05), while the level of IL-35 was negatively correlated with CRP and PCT (r=-0.725, -0.777, P<0.05). Smooth curve fitting showed that sCD14 in peripheral blood after interventional therapy was linearly and positively correlated with the risk of secondary pulmonary infection in NSCLC patients (P<0.05), while IL-35 was linearly and negatively correlated with the risk of secondary pulmonary infection in NSCLC patients (P<0.05). The AUC value of peripheral blood sCD14, IL-35 combined with CRP and PCT in predicting secondary pulmonary infection in NSCLC patients after interventional therapy was as high as 0.930(P<0.05), with good predictive efficacy and significant incremental value. Conclusion: The increase of sCD14 level in peripheral blood of NSCLC patients is linearly and positively correlated with the risk of secondary pulmonary infection after interventional therapy, while the decrease of IL-35 level is linearly and negatively correlated with the risk of secondary pulmonary infection after interventional therapy. Combined detection of sCD14, IL-35, CRP and PCT in peripheral blood after interventional therapy has high predictive value for the risk of secondary pulmonary infection.
2026 Vol. 32 (1): 129-137 [Abstract] ( 24 ) HTML (1 KB)  PDF (1430 KB)  ( 4 )
137 Expression of Cervical KRT17 and ATG12 in Patients with Low-risk and High-risk HPV Infection and Their Influence on Disease Progression
GUO Yanpu, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.022
Objective: To analyze the expression of keratin 17 (KRT17) and autophagy-related protein-12 (ATG12) in cervical local tissues of patients with low-risk and high-risk human papillomavirus (HPV) infection, and their impact on disease progression. Methods: A total of 203 patients with cervical low-grade squamous intraepithelial lesion (LSIL) from December 2020 to December 2022 were enrolled, including 83 cases of low-risk HPV infection and 120 cases of high-risk HPV infection. All cases were followed up for 2 years and divided into progression group and non-progression group according to the progression of the disease within 2 years. Cervical brush specimens were collected at the initial diagnosis, and the expression levels of KRT17 mRNA and ATG12 mRNA were detected by quantitative real-time fluorescence PCR. The levels of KRT17 mRNA and ATG12 mRNA between patients with low-risk and high-risk HPV infection were compared. Point-biserial correlation analysis was used to analyze the correlation between the levels of KRT17 mRNA and ATG12 mRNA and the type of HPV infection. The clinical data, KRT17 mRNA, and ATG12 mRNA levels were compared between the progression group and the non-progression group. Multivariate unconditional Logistic regression analysis was used to analyze the influencing factors of disease progression in patients with LSIL under different HPV status. Logistic (OR) analysis was used to investigate whether there was an additive interaction between KRT17 mRNA and ATG12 mRNA in the progression of LSIL in patients with high-risk HPV infection, and to determine the statistical type of interaction. Receiver operating characteristic (ROC) curve was used to analyze the efficacy of KRT17 mRNA, ATG12 mRNA, and their combination in predicting the progression of LSIL. Results: The expression level of KRT17 mRNA in the high-risk HPV infection group was significantly higher than that in the low-risk HPV infection group (1.35±0.42 vs 0.61±0.20, t=-20.666, P<0.001), while the expression level of ATG12 mRNA was significantly decreased (0.48±0.12 vs 0.80±0.24, t=11.911, P<0.001). Point-biserial correlation analysis showed that KRT17 mRNA was positively correlated with the type of HPV infection (r=0.862, P<0.001). ATG12 mRNA was negatively correlated with HPV infection type (r=-0.799, P<0.001). The proportion of patients with more than one sexual partner, the proportion of patients taking contraceptives frequently, the proportion of patients with high-risk HPV infection, KRT17 mRNA and ATG12 mRNA in the progression group were 15.38%, 23.08%, 97.44%, (1.35±0.30) and (0.33±0.11), respectively, compared with those in the non-progression group 3.05%, 4.27%, 27.44%, (0.98±0.31), (0.68±0.22), the differences were statistically significant (t/χ2= 9.580, 12.870, 61.008, 6.740, -9.640, P= 0.008, <0.001, <0.001, <0.001, <0.001). Multivariate unconditional Logistic regression analysis showed that after adjusting for the number of sexual partners and regular use of contraceptives, KRT17 mRNA and ATG12 mRNA were independent influencing factors for the progression of LSIL in all patients. With each 1 unit increase in these two factors, the risk of progression of LSILwas increased by 1.404 and 0.517 times, respectively (P<0.05). After stratification according to HPV infection type, KRT17 mRNA and ATG12 mRNA were still independent influencing factors for the progression of LSIL in patients with high-risk HPV infection (P<0.05), but in patients with low-risk HPV infection, KRT17 mRNA and ATG12 mRNA were not correlated with the progression of LSIL(P>0.05). The interaction analysis showed that after correcting for the number of sexual partners and regular use of contraceptives, S=1.874, RERI=1.594, and AP=0.361, suggesting that KRT17 mRNA and ATG12 mRNA had a statistically additive synergistic interaction on the progression of the disease; ROC analysis showed that the combined AUC was significantly greater than that of KRT17 mRNA and ATG12 mRNA alone (P<0.05). Conclusion: High expression of KRT17 and low expression of ATG12 synergistically promote the progression of LSIL in patients with high-risk HPV infection. Combined detection of KRT17 and ATG12 can be used as an effective risk warning tool, providing a new basis for precise management and intervention of LSIL.
2026 Vol. 32 (1): 137-144 [Abstract] ( 16 ) HTML (1 KB)  PDF (1335 KB)  ( 3 )
144 Pathogenetic Characteristics of Paediatric Infectious Mononucleosis Based on Pathogen-Targeted Sequencing Technology and Correlation Analysis with EB Virus Viral Load
ZHENG Zheng, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.023
Objective: To detect pathogens in children with infectious mononucleosis (IM) based on pathogen-targeted sequencing (tNGS) technology, and to analyze their pathogenic characteristics and the correlation with EBV viral load. Methods: The observation group consisted of 46 children with IM caused by EBV who were admitted to the First People's Hospital of Tongling and Tongling Maternal and Child Health Hospital between June 2023 and December 2024. The control group consisted of 95 non-IM children who were admitted to our hospital during the same time period.. The tNGS was used to detect pathogens in the two groups of patients and to compare the pathogenetic characteristics of the two groups. Results: Using the tNGS technique, 32 pathogens, including 13 bacteria, 18 viruses, and 1 mycoplasma, were detected in the control group, while 24 pathogens, including 12 bacteria, 11 viruses, and 1 chlamydia, were detected in the observation group. Among the pathogens isolated using the tNGS technique, the top 3 bacterial detections in the control group were Haemophilus influenzae (n=40), Streptococcus pneumoniae (n=29), and Acinetobacter baumannii (n=31), while in the observation group the top 3 bacteria were Haemophilus influenzae (n=29), Streptococcus pneumoniae (n=22), and Staphylococcus aureus (n=21). EBV was detected in both groups, and the EBV viral load in the observation group was higher than that in the control group (P<0.05). According to correlation analysis results, the EBV virus load was positively connected with Staphylococcus aureus, Moraxella catarrhalis, Acinetobacter baumannii, Klebsiella pneumoniae, and Streptococcus angina.(P<0.05). Conclusion: tNGS technology facilitates a comprehensive understanding of the pathogenetic characteristics in children with IM, and these pathogenetic features correlate with EBV viral load, thereby providing a basis for clinical diagnosis and treatment.
2026 Vol. 32 (1): 144-150 [Abstract] ( 20 ) HTML (1 KB)  PDF (1573 KB)  ( 4 )
150 Role of miR-155 in Regulating PAD4 Expression in Autophagy and Proliferation of Gastric Cancer Cells and Its Clinical Relevance
QU Zhenjie, CUI Qin
DOI: 10.3969/j.issn.1006-6233.2026.01.024
Objective: To investigate the mechanism of action and clinical relevance of miR-155 and PAD4 in autophagy and proliferation of gastric cancer cells. Methods: Tumor tissue and adjacent non-tumor tissue from 20 gastric cancer patients were collected. The expression differences of miRNA and mRNA between tumor tissue and adjacent tissue were compared using miRNA-seq and RNA-seq technologies, followed by KEGG pathway analysis of differentially expressed mRNA. The expression levels of miR-155, PAD4, and Beclin-1 in tumor tissue and adjacent tissue were detected by quantitative reverse transcription PCR (qRT-PCR). Correlation analysis was conducted on the expression of miR-155, PAD4, and Beclin-1 in tumor tissue. Further analysis was performed on the association between the expression level of miR-155 and patients' gender, age, and tumor malignancy degree. The targeting relationship between miR-155 and PAD4 was determined by luciferase reporter assay. MGC803 cells were transfected with si-NC, si-miR-155, si-miR-155+si-NC, and si-miR-155+si-PAD4, and the expression of miR-155, PAD4, Beclin-1, and p62 was detected by qRT-PCR. Cell proliferation levels were assessed using the CCK-8 method. Results: Compared to adjacent tissues, 295 miRNAs and 348 mRNAs were upregulated, while 195 miRNAs and 174 mRNAs were downregulated in tumor tissues. Upregulated mRNAs were primarily involved in cell proliferation-related signaling pathways such as MAPK and Wnt. In tumor tissues, the expression of miR-155 and Beclin-1 was decreased, whereas PAD4 expression was increased (P<0.05). There was a positive correlation between the expression of miR-155 and Beclin-1, and a negative correlation with PAD4 expression (P<0.05). The expression level of miR-155 was significantly related to the degree of tumor malignancy (P<0.05) but not significantly associated with patients' gender and age (P>0.05). The experimental results indicated that miR-155 could target PAD4. Compared to the si-NC group, the si-miR-155 group showed decreased expression of miR-155 and Beclin-1, and increased expression of PAD4 and p62, resulting in higher cell proliferation levels (P<0.05). Compared to the si-miR-155+si-NC group, the si-miR-155+si-PAD4 group showed reduced expression of PAD4 and p62, increased expression of Beclin-1, and decreased cell proliferation levels (P<0.05). Conclusion: miR-155 promotes autophagy and inhibits proliferation levels in gastric cancer cells by targeting and suppressing the expression of PAD4.
2026 Vol. 32 (1): 150-156 [Abstract] ( 24 ) HTML (1 KB)  PDF (1520 KB)  ( 10 )
157 Comparison of Nimotuzumab Combined with Radiotherapy and Hyperthermia versus Concurrent Chemoradiotherapy in the Treatment of Elderly Patients with Locally Advanced Cervical Cancer
YANG Jiyuan, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.025
Objective: To investigate the efficacy and safety of Nimotuzumab combined with radiotherapy and hyperthermia in the treatment of elderly patients with locally advanced cervical cancer. Methods: The retrospective study included 62 elderly patients with locally advanced cervical cancer treated in the Oncology Department of the Affiliated Hospital of Chengde Medical University from January 2021 to December 2022. The observation group (31 cases) received Nimotuzumab injection combined with radiotherapy and hyperthermia, while the control group (31 cases) received concurrent chemoradiotherapy. Short-term and long-term efficacy, as well as adverse reactions, were evaluated. Results: In the observation group, 23 patients achieved complete response (CR), 6 achieved partial response (PR), 1 had stable disease (SD), and 1 had progressive disease (PD), yielding an objective response rate (ORR) of 96.77%. In the control group, 21 achieved CR, 5 PR, 2 SD, and 3 PD, with an ORR of 90.32%. There was no statistically significant difference in ORR between the two groups. For long-term outcomes, the 2-year progression-free survival (PFS) and overall survival (OS) rates in the observation group were 87.10% and 96.77%, respectively, compared to 80.65% and 90.32% in the control group, with no significant differences. Post-treatment immune function analysis showed that CD4+ cell counts and the CD4+/CD8+ ratio increased in both groups, with a more pronounced elevation in the observation group (P<0.05). The main adverse reactions included neutropenia, diarrhea, urinary tract irritation symptoms, anemia, and mucositis. The observation group primarily experienced Grade 1 to 2 adverse reactions, with no Grade 3 or higher adverse reactions occurring. In contrast, the control group experienced grade 3 diarrhea and urinary tract irritation symptoms. The incidence of diarrhea and urinary tract irritation was significantly lower in the observation group (P<0.05). Conclusion: Nimotuzumab combined with radiotherapy and hyperthermia demonstrates comparable efficacy to concurrent chemoradiotherapy in treating elderly patients with locally advanced cervical cancer but with significantly reduced adverse reactions.
2026 Vol. 32 (1): 157-163 [Abstract] ( 27 ) HTML (1 KB)  PDF (1238 KB)  ( 6 )
163 Comparative Study of NCPAP Model or NIPPV Model Combined with Bovine Pulmonary Surfactant in Treating Neonatal Respiratory Distress Syndrome
ZHANG Rui, HUANG Jin, LI Yingying, et al
DOI: 10.3969/j.issn.1006-6233.2026.01.026
Objective: To analyze the effect of nasal continuous positive airway pressure (NCPAP) or nasal intermittent positive pressure ventilation (NIPPV) combined with bovine pulmonary surfactant on neonatal respiratory distress syndrome (NRDS). Methods: 120 children with respiratory distress syndrome, born in the hospital from June 2022 to May 2024, were divided into the NCPAP group (n=58, NCPAP mode + bovine lung surfactant) and the NIPPV group (n=62, NIPPV mode + bovine lung surfactant) according to the treatment methods. The noninvasive ventilation time, total oxygen use time and hospitalization time were compared between both groups. The arterial blood gas indexes and inflammatory response indexes in the two groups were observed before and after treatment, and the complications were compared. Results: NIPPV group showed shorter noninvasive ventilation time and hospitalization time than NCPAP group (P<0.05). There was no statistically significant difference in the total oxygen usage time between the two groups (P>0.05). The arterial partial pressure of carbon dioxide (PaCO2) in NIPPV group at 24 hours after treatment was lower while the arterial partial pressure of oxygen (PaO2) and pH value were higher compared to NCPAP group (P<0.05). The levels of interleukin-6 (IL-6), procalcitonin (PCT) and C-reactive protein (CRP) in two groups were decreased at 3 days after treatment (P<0.05), and the above levels exhibited no statistical differences between groups (P>0.05). The differences in complications were not statistically significant between groups (P>0.05). Conclusion: Compared with NCPAP model combined with bovine pulmonary surfactant, NIPPV model combined with bovine pulmonary surfactant can better shorten the NRDS noninvasive ventilation time and hospitalization time, and improve the blood gas indexes.
2026 Vol. 32 (1): 163-167 [Abstract] ( 21 ) HTML (1 KB)  PDF (1116 KB)  ( 13 )
167 Evaluation of the Severity of Mycoplasma Pneumonia and Diagnostic Efficacy of Bacterial Infection in Children by Inflammatory Indexes
WANG Gang, LI Weichun, SHI Daqing
DOI: 10.3969/j.issn.1006-6233.2026.01.027
Objective: To explore the diagnostic value of inflammatory indicators for the severity of mycoplasma pneumonia in children and the diagnosis of concurrent bacterial infection. Methods: A total of 105 children with mycoplasma pneumonia admitted to the hospital from January 2023 to December 2024 were retrospectively selected as the study objects. According to the severity of mycoplasma pneumonia in children, the patients were divided into severe group (n=42) and mild group (n=63). At the same time, all patients in the severe group and the mild group were divided into the infected group (n=37) and the non-infected group (n=68) according to whether there was co-bacterial infection. The clinical data and laboratory results of the children were collected, and the independent risk factors for the severity of mycoplasma pneumonia in children were investigated by univariate analysis, correlation analysis and multiple logistic regression analysis. The receiver operating characteristic curve (ROC) was used to evaluate the efficacy of inflammatory indicators in the combined diagnosis of concurrent bacterial infection. Results: The results of the univariate analysis of the influencing factors of the severity of pediatric mycoplasma pneumonia indicated that there were statistically significant differences in inflammatory indicators between the severe group and the mild group (P<0.05). The results of multivariate Logistic regression analysis showed that PCT and IL-6 were the influencing factors for evaluating the severity of mycoplasma pneumonia in children (P<0.05); the results of the univariate analysis of pediatric mycoplasma pneumonia combined with bacterial infection indicated that there were statistically significant differences in inflammatory indicators between the infected group and the uninfected group (all P<0.05). The multivariate Logistic regression analysis of the influencing factors of pediatric mycoplasma pneumonia combined with bacterial infection showed that NLR, CRP, IL-6, LDH, and WBC were independent risk factors for evaluating pediatric mycoplasma pneumonia combined with bacterial infection (P<0.05). The ROC curve was drawn, and the results showed that the areas under the curve (AUC) of NLR, CRP, IL-6, LDH, and WBC levels for predicting were 0.649, 0.644, 0.645, 0.636, and 0.656 respectively, and the combined prediction AUC was 0.874, indicating a relatively high diagnostic value. Conclusion: Inflammatory indicators can help medical doctors diagnose the severity of mycoplasma pneumonia in children and whether there is bacterial infection. Compared with a single indicator, multiple indicators combined with bacterial infection are more effective in the diagnosis of mycoplasma pneumonia in children.
2026 Vol. 32 (1): 167-174 [Abstract] ( 25 ) HTML (1 KB)  PDF (1280 KB)  ( 7 )
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