慢性乙型肝炎患者HBV-DNA载量血清表面抗原miR-122miR-21表达水平及其临床应用价值

doi:10.3969/j.issn.1006-6233.2025.11.025

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摘要 目的: 探究慢性乙型肝炎(CHB)患者乙型肝炎病毒(HBV)-DNA载量、血清表面抗原(HBsAg)、miR-122、miR-21表达水平及其临床应用价值。方法: 选取2019年12月至2024年7月收治的120例CHB患者,根据HBV-DNA载量不同分为低载量组(n=52)与高载量组(n=68),对比两组HBV-DNA载量、HBsAg、miR-122、miR-21表达水平,采用Pearson法分析HBV-DNA载量与HBsAg、miR-122、miR-21表达水平的相关性。根据肝组织病理学诊断标准炎症分级将患者分为轻度组(n=48)与中重度组(n=72),对比两组HBV-DNA载量、HBsAg、miR-122、miR-21表达水平;采用二元Logistics方程分析影响患者肝组织炎症活动度的相关影响因素,采用ROC曲线分析HBV-DNA载量、HBsAg、miR-122、miR-21表达水平对肝组织炎症活动度的诊断价值。结果: 高载量组HBsAg、miR-122、miR-21表达水平均显著高于低载量组(P<0.05);Pearson相关分析,HBsAg、miR-122、miR-21表达水平与HBV-DNA载量呈正相关(P<0.05);中重度组HBV-DNA载量、HBsAg、miR-122、miR-21表达水平均显著高于轻度组(P<0.05);多因素Logistic回归分析的结果显示,HBV-DNA载量、HBsAg、miR-122、miR-21表达水平均与患者肝组织炎症活动度有关(P<0.05);HBV-DNA载量、HBsAg、miR-122、miR-21表达水平预测肝组织炎症活动度的AUC分别为0.918、0.706、0.768、0.749;四项联合诊断患者肝组织炎症活动度的AUC为0.960,联合诊断价值更高。结论: 严重CHB以HBV-DNA载量、血清表面抗原、miR-122、miR-21高表达水平为临床特征,该类指标有望成为预测肝组织炎症活动度的生物标志物。

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	刘丽娜
	梁永纲
	李丽

关键词 ：慢性乙型肝炎, 病毒载量, 抗原, 微小RNA, 肝组织炎症活动度

Abstract：Objective: To investigate the hepatitis B virus (HBV)-DNA load and expression levels of serum surface antigen (HBsAg), miR-122 and miR-21 in patients with chronic hepatitis B (CHB) and study their correlation. Methods: A total of 120 CHB patients admitted from December 2019 to July 2024 were selected and divided into low load group (n=52) and high load group (n=68) according to different HBV-DNA loads, and the HBV-DNA load and expression levels of HBsAg, miR-122 and miR-21 were compared between groups. Pearson method was used to analyze the correlation between HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels. The patients were classified into mild group (n=48) and moderate-to-severe group (n=72) according to the inflammation grading of liver histopathological diagnostic criteria, and the HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels were compared. Binary logistics equation analysis was used to analyze the related influencing factors affecting the inflammatory activity of liver tissue. ROC curve was used to analyze the diagnostic value of HBV-DNA load, HBsAg, miR-122 and miR-21 on the inflammatory activity of liver tissue. Results: The miR-122 and miR-21 in high load group were significantly higher than those in low load group (P<0.05). Pearson correlation analysis showed that the expression levels of HBsAg, miR-122 and miR-21 were positively correlated with HBV-DNA load (P<0.05). The HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels were significantly higher in moderate-to-severe group than those in mild group (P<0.05). The results of multiple logistic regression analysis show that HBV-DNA load and HBsAg, miR-122 and miR-21 expression levels were correlated with hepatitis activity (P<0.05). The AUCs of HBV-DNA load, HBsAg, miR-122 and miR-21 in predicting liver tissue inflammatory activity were 0.918, 0.706, 0.768, and 0.749, respectively. The AUC of the four combined diagnosis of liver tissue inflammatory activity was 0.960, and the combined diagnostic value was higher. Conclusion: Severe CHB is characterized by high HBV-DNA load, serum surface antigen, miR-122 and miR-21, and these indicators are expected to be biomarkers for predicting inflammatory activity of liver tissue.

Key words： Chronic hepatitis B Viral load Antigen MicroRNA Inflammatory activity of liver tissue

基金资助:内蒙古自治区科技计划项目,(编号:2020GG0085)

通讯作者: 李丽

引用本文:

刘丽娜, 梁永纲, 李丽. 慢性乙型肝炎患者HBV-DNA载量血清表面抗原miR-122miR-21表达水平及其临床应用价值[J]. 河北医学, 2025, 31(11): 1907-1912.
LIU Lina, LIANG Yonggang, LI Li. Study on HBV-DNA Load Serum Surface Antigen miR-122 and miR-21 Expression Levels and Their Correlation in Patients with Chronic Hepatitis B. HeBei Med, 2025, 31(11): 1907-1912.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.11.025 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I11/1907

[1] Shi YW,Yang RX,Fan JG.Chronic hepatitis B infection with concomitant hepatic steatosis: current evidence and opinion[J].World Gastroenterol,2021,27(26):3971-3983.
[2] Mao X,Mak LY,Seto WK.The latest evidence on the impact of fatty liver on liver-related outcomes and mortality in chronic hepatitis B[J].Clin Mol Hepatol,2023,29(3):690-692.
[3] 谢露,刘光伟,郭会军.HBV DNA载量水平与乙型肝炎相关肝癌指标的相关性分析及其对预后的影响[J].中西医结合肝病杂志,2023,33(10):925-929.
[4] McCoullough LC,Fareh M,Hu W,et al.CRISPR-Cas13b-mediated suppression of HBV replication and protein expression[J].J Hepatol,2024,81(5):794-805.
[5] Mokhtari F,Kaboosi H,Mohebbi SR,et al.Circulating plasma miR-122 and miR-583 levels are involved in chronic hepatitis B virus pathogenesis and serve as novel diagnostic biomarkers[J].Genet Test Mol Biomarkers,2023,27(8):232-238.
[6] Tan B,Liu M,Wang L,et al.Serum microRNAs predict response of patients with chronic hepatitis B to antiviral therapy[J].Int Infect Dis,2021,108(1):37-44.
[7] 中华医学会感染病学分会,中华医学会肝病学分会.慢性乙型肝炎防治指南(2019年版)[J].中华肝脏病杂志,2019,27(12):938-961.
[8] 高兴娟,程磊,马秀清,等.血清preS1水平与慢性乙型肝炎患者肝纤维化的关系及对癌变的诊断价值[J].传染病信息,2024,37(2):132-136.
[9] Zhang C,Yang Z,Wang Z,et al.HBV DNA and HBsAg: early prediction of response to peginterferon α-2a in HBeAg-negative chronic hepatitis B[J].Int Med Sci,2020,17(3):383-389.
[10] Ning J,Wang J,Zheng H,et al.Solely HBsAg intrauterine exposure accelerates HBV clearance by promoting HBs-specific immune response in the mouse pups[J].Emerg Microbes Infect,2022,11(1):1356-1370.
[11] Gozlan Y,Aaron D,Davidov Y,et al.HBV-RNA,quantitative HBsAg,levels of HBV in peripheral lymphocytes and HBV mutation profiles in chronic hepatitis B[J].Viruses,2022,14(3):584-595.
[12] Singh SP,Maurya V,Kumar K,et al.Serum expression level of microRNA-122 and its significance in different stages of hepatitis B virus infection[J].Cell Mol Biol (Noisy-le-grand),2023,69(6):36-40.