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2025 Vol. 31, No. 9 Published: 2025-09-30 1409 Effect of Sulforaphane on Intestinal Inflammation and Gut Microbiota in Neonatal Rats with Necrotizing Enterocolitis by Adjusting AMPK/SIRT1 Pathway ZHAO Le, SUN Jie, LI Haixia, et al DOI: 10.3969/j.issn.1006-6233.2025.09.01 Objective: To investigate the effect of sulforaphane (SFN) on intestinal inflammation and gut microbiota in neonatal rats with necrotizing enterocolitis (NEC) by adjusting AMP-activated protein kinase (AMPK)/silent information regulator 1 (SIRT1) pathway. Methods: Newborn rats were stochastically assigned into the normal group (NC group), NEC model group (NEC group), low-dose SFN group (L-SFN group), high-dose SFN group (H-SFN group), and high-dose SFN+AMPK inhibitor BML-275 group (H-SFN+BML-275 group). Except for the normal group, NEC models were established in all other groups. ELISA was used to detect tumor necrosis factor-α (TNF-α), interleukin-1β, and IL-6 in serum. HE staining was used to observe pathological changes in ileocecal intestinal tissue. TUNEL staining was performed to detect apoptosis of ileocecal intestinal tissue cells. Western blot was performed to detect p-AMPK, AMPK, and SIRT1 in ileocecal intestinal tissue. Selective culture medium was used to analyze changes in gut microbiota such as lactobacilli, bifidobacteria, and Escherichia coli in feces. Results: For the NC group, the NEC group showed an increase in TNF-α, IL-1β, and IL-6 in serum of neonatal rats, an increase in the pathological damage score and fluorescence intensity of cell apoptosis in the ileocecal intestinal tissue, a decrease in the expression of p-AMPK/AMPK and SIRT1, a decrease in lactobacilli and bifidobacteria in feces, and an increase in Escherichia coli (P<0.05). For the NEC group, the L-SFN group and H-SFN group showed a decrease in TNF-α, IL-1β, and IL-6 in serum of neonatal rats, a decrease in the pathological damage score and apoptosis fluorescence intensity of the ileocecal intestinal tissue, an increase in the expression of p-AMPK/AMPK and SIRT1, an increase in lactobacilli and bifidobacteria in feces, and a decrease in Escherichia coli (P<0.05). For the H-SFN group, the H-SFN+BML-275 group showed an increase in TNF-α, IL-1β, and IL-6 in serum of neonatal rats, an increase in the pathological damage score and fluorescence intensity of cell apoptosis in the ileocecal intestinal tissue, a decrease in the expression of p-AMPK/AMPK and SIRT1, a decrease in lactobacilli and bifidobacteria in feces, and an increase in Escherichia coli (P<0.05). Conclusion: SFN can alleviate intestinal inflammatory response and improve gut microbiota composition in NEC neonatal rats by activating AMPK/SIRT1 pathway, ultimately slowing down the progression of NEC disease. 2025 Vol. 31 (9): 1409-1415 [Abstract] ( 184 ) HTML (1 KB)  PDF (2168 KB)  ( 36 ) 1415 Effect of Plantain Seed Polysaccharide on Renal Tissue Injury in Diabetic Nephropathy Rats by Regulating Sirt1-Foxo1 Signaling Pathway LIU Sufang, ZHAO Hua DOI: 10.3969/j.issn.1006-6233.2025.09.02 Objective: To investigate the effect of plantain seed polysaccharide (PSP) on renal tissue injury in diabetic nephropathy (DN) rats by regulating Sirt1-Foxo1 signaling pathway. Methods: A DN rat model was constructed, and successfully modeled rats were assigned into DN group, L-PSP, H-PSP groups (gavage of 400 and 800mg/kg PSP), and H-PSP+EX-527 group (gavage of 800mg/kg PSP+gavage of 5 mg/kg Sirt1 inhibitor EX-527), with 10 rats in each group. Another 10 normal rats were regarded as Control group. The Control group and DN group were given equal amounts of physiological saline once a day for 28 consecutive days. Use a urine protein assay kit to detect 24-hour urinary protein levels; and employ ELISA to measure serum creatinine (SCr) andβ2-microglobulin (β2-MG) levels in rats. HE staining was performed to observe pathological changes in rat kidney tissue. The chemical colorimetric method was performed to detect SOD, MDA, and GSH-Px in rat kidney tissue. ELISA was performed to detect IL-6, TNF-α, and IL-8 in rat serum. Western blot was performed to detect changes in the expression of Sirt1-Foxo1 signaling pathway proteins in rat kidney tissue. Results: Compared with the Control group, the renal tissue of rats in DN group lost normal structure, showed great interstitial inflammatory cells infiltration, enlarged glomeruli, obvious tubular vacuolization with degenerat and ionnecrosis, and increased mesangial matrix; the SCr, β2-MG, 24-hour urinary protein, MDA, IL-6, TNF-α, and IL-8 elevated; and the SOD, GSH-Px, and the Sirt1 and Foxo1 protein expression decreased (P<0.05). Compared with the DN group, the degree of interstitial inflammation cells infiltration in the renal tissue of rats in L-PSP and H-PSP groups decreased, the glomerular structure was basically normal, and the number of vacuoles decreased; the SCr, β2-MG, 24-hour urinary protein, MDA, IL-6, TNF-α, and IL-8 decreased; and the SOD, GSH-Px, and the Sirt1 and Foxo1 protein expression increased (P<0.05). Compared with the H-PSP group, the H-PSP+EX-527 group showed aggravated inflammatory infiltration and increased vacuoles in the renal tissue of rats; the SCr, β2-MG, 24-hour urinary protein, MDA, IL-6, TNF-α, and IL-8 elevated; and the SOD, GSH-Px, and the Sirt1 and Foxo1 protein expression decreased (P<0.05). Conclusion: PSP can alleviate renal injury in DN rats, and its mechanism may be achieved by activating Sirt1-Foxo1 signaling pathway, enhancing the body's antioxidant capacity, and reducing inflammatory factors such as IL-6, TNF-α, and IL-8. 2025 Vol. 31 (9): 1415-1420 [Abstract] ( 138 ) HTML (1 KB)  PDF (1580 KB)  ( 20 ) 1420 Exploring the Effects of Taohe Chengqi Decoction on Endometriosis Rats through wnt/β-catenin Pathway and Its Mechanisms MA Jinhui, YU Yuexin, ZHANG Mimi, et al DOI: 10.3969/j.issn.1006-6233.2025.09.03 Objective: To explore the effects of Taohe Chengqi decoction (THCQD) on endometriosis (EMs) rats through Wnt/β-catenin pathway and its mechanisms. Methods: An EMs rat model was constructed, and rats were randomly assigned into EMs group (Model group), L-THCQD (THCQD low dose) group, M-THCQD (THCQD medium dose) group, H-THCQD (THCQD high dose) group (The rats in these groups were fed 1.89, 3.78 and 7.56 g/kg THCQD, respectively), and LiCl group (orally administered 7.56 g/kg THCQD and orally administered 100mg/kg Wnt/β-catenin signaling pathway activator LiCl). Each group had 10 rats. Additionally, 10 rats were recruited as the NC group (Control group). The NC group and the EMs group were given equal volumes of sodium chloride solution. The volume and mass of ectopic lesions in rats were measured. HE staining was used to observe pathological changes in rat ectopic endometrial tissue. Immunohistochemistry was used to measure Ki67 and vascular endothelial growth factor (VEGF) in ectopic endometrial tissue. TUNEL staining was used to observe apoptosis in rat ectopic endometrial tissue. ELISA was performed to measure the inflammatory factors and sex hormones in serum. Western blot was performed to measure the Wnt/β-catenin pathway and apoptosis related proteins in rat ectopic endometrial tissue. Results: For the NC group, the EMs group showed severe pathological damage to ectopic endometrial tissue in rats, increased Ki67, VEGF, IL-8, IL-6, IL-18, estradiol (E2), progesterone (P), Wnt, and β-catenin (P<0.05), decrease follicle stimulating hormone (FSH) and luteinizing hormone (LH) (P<0.05), while the apoptosis rate, Bcl-2, and Bax no statistically conspicuous difference (P>0.05). For the EMs group, the L-THCQD group, M-THCQD group, and H-THCQD group showed significant reduction in pathological damage to rat ectopic endometrial tissue, significantly reduced volume, mass, Ki67, VEGF, IL-8, IL-6, IL-18, E2, P, Bcl-2, Wnt, and β-catenin (P<0.05), and significantly increased apoptosis rate and the expression of FSH, LH, and Bax (P<0.05). For the H-THCQD group, the LiCl group had aggravated pathological damage in rat ectopic endometrial tissue, significantly increased volume, mass, Ki67, VEGF, IL-8, IL-6, IL-18, E2, P, Bcl-2, Wnt, and β-catenin (P<0.05), and significantly reduced apoptosis rates and the expression of FSH, LH, and Bax (P<0.05). Conclusion: THCQD has therapeutic effects on EMs rats by inhibiting inflammatory responses and promoting apoptosis of ectopic endometrial tissue, which may be related to the inhibition of Wnt/β-catenin pathway. 2025 Vol. 31 (9): 1420-1427 [Abstract] ( 152 ) HTML (1 KB)  PDF (2993 KB)  ( 17 ) 1428 Effects of LncRNA KCNQ1OT1 on Myocardial Injury in Diabetic Cardiomyopathy Rats by Adjusting miR-324-5p HUANG Liu, WEI Huiqing, YAN Jie, et al DOI: 10.3969/j.issn.1006-6233.2025.09.04 Objective: To explore the effect of long non-coding RNA (LncRNA) KCNQ1 overlapping transcript 1 (KCNQ1OT1) on myocardial damage in diabetic cardiomyopathy (DCM) rats by adjusting microRNA-324-5p (miR-324-5p). Methods: A DCM rat model was established by feeding high glucose and high-fat diet combined with intraperitoneal injection of streptozotocin (STZ). The rats were stochastically divided into DCM group, sh-NC group, sh-KCNQ1OT1 group, sh-KCNQ1OT1+antiagomir-NC group, and sh-KCNQ1OT1+miR-324-5p antiagomir group, each had 12 rats. Another 12 healthy rats were designated as control group and fed with regular food. The blood glucose meter was used to detect the fasting blood glucose (FBG) in each group. Echocardiography was used to detect cardiac function parameters in each group. ELISA was performed to detect the BNP and cTnⅠ in serum. HE staining was performed to observe the pathological changes of myocardial tissue. TUNEL staining was used to detect the apoptosis status of myocardial tissue cells. QRT-PCR was performed to detect the LncRNA KCNQ1OT1 and miR-324-5p in the myocardial tissues. In addition, dual luciferase reporter gene assay was used to detect the targeting relationship between LncRNA KCNQ1OT1 and miR-324-5p. Results: The DCM group had conspicuously lower LVFS, LVEF, LVSP, and miR-324-5p, and conspicuously higher LVSD, FBG, BNP, cTnⅠ, cell apoptosis rate, and LncRNA KCNQ1OT1 than control group (P<0.05). The sh-KCNQ1OT1 group had conspicuously higher LVFS, LVEF, LVSP, and miR-324-5p, and conspicuously lower LVSD, FBG, BNP, cTnⅠ, cell apoptosis rate, and LncRNA KCNQ1OT1 than the DCM and sh-NC groups (P<0.05). The sh-KCNQ1OT1+miR-324-5p antiagomir group had conspicuously lower LVFS, LVEF, LVSP, and miR-324-5p, and conspicuously higher LVSD, FBG, BNP, cTnⅠ, cell apoptosis rate, and LncRNA KCNQ1OT1 than the sh-KCNQ1OT1 group and sh-KCNQ1OT1+antiagomir-NC (P<0.05). And the dual luciferase reporter gene experiment confirmed that LncRNA KCNQ1OT1 could target the inhibition of miR-324-5p (P<0.05). Conclusion: Interference with LncRNA KCNQ1OT1 can target upregulation of miR-324-5p to improve myocardial damage in DCM rats. 2025 Vol. 31 (9): 1428-1434 [Abstract] ( 145 ) HTML (1 KB)  PDF (1899 KB)  ( 19 ) 1434 Study on the Regulatory Mechanism of Macrophage-Derived Exosomes on GSDMD-Mediated Pyroptosis in Rats with Osteomyelitis CHAI Hao, XU Bin, TAYIERJIANG Yasheng DOI: 10.3969/j.issn.1006-6233.2025.09.05 Objective: To explore the regulatory effects of macrophage-derived exosomes (Exo) on gasdermin D (GSDMD)-mediated pyroptosis in osteomyelitis (OM) rats. Methods: Macrophage-derived exosomes were isolated and characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blot to detect exosomal markers cluster of differentiation (CD) 9, CD63, and tumor susceptibility gene 101 (TSG101) proteins. Additionally, 48 adult male SD rats were randomly divided into four groups: normal control (NC) group with no treatment; OM group, where an osteomyelitis rat model was established; macrophage exosome treatment group (OM+Exo group), where macrophage-derived exosomes were administered to the OM model; and macrophage exosome combined with GSDMD inhibitor LDC7559 treatment group (OM+Exo+LDC7559 group), where LDC7559 was administered in addition to macrophage-derived exosomes, with n=12 rats per group. Changes in bone tissue pathological damage scores in osteomyelitis rats were observed. Western blot was used to detect the expression levels of pyroptosis marker proteins [activated caspase-1, NLR Family Pyrin Domain Containing 3 (NLRP3), interleukin (IL)-1β, IL-18, GSDMD] and inflammatory cytokines tumor necrosis factor-alpha (TNF-α), IL-6, IL-10. Enzyme-linked immunosorbent assay (ELISA) was used to measure oxidative stress indicators malondialdehyde (MDA) and superoxide dismutase (SOD) levels in bone tissue. Results: Transmission electron microscopy characterization showed that macrophage-derived exosomes are spherical vesicles with a particle size of (42.53~151.65) nm and a Zeta potential of (-29.36±4.08) mV, and they prominently express CD9, CD63, and TSG101. Compared to the NC group, the OM group showed significantly higher bone tissue damage scores, activated caspase-1, NLRP3, IL-1β, IL-18, GSDMD protein expression, TNF-α, IL-6 protein expression, and MDA levels, with significantly lower IL-10 protein expression and SOD levels (all P<0.05). Compared to the OM group, the OM+Exo group showed significant downregulation of these indicators, with significant upregulation of IL-10 protein expression and SOD levels (all P<0.05). Compared to the OM+Exo group, the OM+Exo+LDC7559 group showed further downregulation of these indicators and further upregulation of IL-10 protein expression and SOD levels (all P<0.05). Conclusion: Macrophage-derived exosomes alleviate bone tissue damage in osteomyelitis rats by downregulating GSDMD-mediated pyroptosis and inflammatory cytokine expression, and by mitigating oxidative stress response, indicating their potential value in osteomyelitis treatment. 2025 Vol. 31 (9): 1434-1439 [Abstract] ( 125 ) HTML (1 KB)  PDF (1762 KB)  ( 10 ) 1439 Effect of Vitamin D on Hypoxic-Ischemic Brain Damage in Neonatal Rats and Its Mechanism LI Feng, WEI Yong, WANG Zhenling, et al DOI: 10.3969/j.issn.1006-6233.2025.09.06 Objective: To investigate the effect of vitamin D on hypoxic-ischemic brain damage(HIBD) in neonatal rats and its possible mechanism. Methods: A total of 54 neonatal rats were randomly divided into control-48h, control-72h, HIBD-48h, HIBD-72h, treatment-48h and treatment-72h groups, with 9 rats in each group. The HIBD model was established by double ligation of the right common carotid artery. The neonatal rats in the treatment group were treated with vitamin 24 or 48 hours after the establishment of the model, and were killed 24 hours after the drug administration. The neonatal rats in each group were divided into 48h and 72h subgroups. Kits were used to detect the content of 1, 25-dihydroxyvitamin D3 (1,25(OH)2D3), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in brain tissue. Hematoxylin-eosin (HE) staining was used to detect the neuronal status. RT-qPCR and Western blot were used to detect the mRNA and protein expression of acid sensitive ion channel 1a (ASIC1a) and vitamin D receptor (VDR) genes. Results: With the extension of the model construction time, the content of 1,25(OH)2D3 and SOD activity in the brain of the neonatal rats gradually decreased, the content of MDA increased, the pH value decreased, the mRNA content and protein expression of VDR decreased, and the mRNA content and protein expression of ASIC1a increased. Compared with the control group, the contents of 1,25(OH)2D3 and the activity of SOD in the brain of neonatal rats in the HIBD group were decreased (P<0.05), MDA contents were increased (P<0.05), pH values were decreased (P<0.05), and VDR mRNA and protein expressions were decreased (P<0.05), ASIC1a mRNA and protein expressions were increased (P<0.05). HE staining showed that the neuronal state was obviously damaged. Compared with the HIBD group, the treatment group had increased 1,25(OH)2D3 content and SOD activity in the brain (P<0.05), MDA content decreased (P<0.05), increased pH value (P<0.05), increased VDR mRNA and protein expression (P<0.05), decreased ASIC1a mRNA and protein expression (P<0.05), and HE staining showed that the state of neurons was significantly recovered. Conclusion: Vitamin D can improve neuronal injury in neonatal rats with HIBD by increasing the expression of VDR, restoring brain pH value, reducing the expression of ASIC1a protein, and reducing oxidative stress. 2025 Vol. 31 (9): 1439-1446 [Abstract] ( 147 ) HTML (1 KB)  PDF (1722 KB)  ( 12 ) 1446 Effects of Lidocaine on the Proliferation Invasion and Vascular Mimicry of Esophageal Cancer Cells by Inhibiting the RhoA/ROCK Pathway NITeweibuzi, CHEN Yong, ZHANG Yufeng, et al DOI: 10.3969/j.issn.1006-6233.2025.09.07 Objective: To explore the effects of lidocaine on the proliferation, invasion, and vascular mimicry of esophageal cancer (EC) cells by inhibiting the small GTPase (RhoA)/Rho-associated coiled-coil containing protein kinase (ROCK) pathway. Methods: The human EC cell line Eca-109 was cultured in vitro and assigned into NC group, low-dose lidocaine group (lidocaine 5 mmol/L), medium dose lidocaine group (lidocaine 10mmol/L), high-dose lidocaine group (lidocaine 15 mmol/L), and high-dose lidocaine+RhoA/ROCK pathway activator (LPA) group (lidocaine 15 mmol/L+50μmol/L LPA). The clone formation experiment, Transwell chamber experiment, and Matrigel lumen formation experiment were used to detect the proliferation and invasion abilities, and angiogenesis of Eca-109 cells in each group. The qRT-PCR experiment was used to detect the RhoA and ROCK mRNA of Eca-109 cells in each group. Western blot was used to detect the RhoA, ROCK, CCND1, Myc, VEGF, and TIMP-1 proteins of Eca-109 cells. Results: Compared with the NC group, the low, medium, and high dose lidocaine groups showed a decrease in the numbers of Eca-109 cell colony formation, invasion, and angiogenesis tubular structures, reduced RhoA, ROCK mRNA, and their proteins, and prominently decreased CCND1, Myc, and VEGF proteins, and prominently increased TIMP-1 protein (P<0.05). Compared with the high-dose lidocaine group, the high-dose lidocaine+LPA group showed an increase in the numbers of Eca-109 cell colony formation, invasion, and angiogenesis tubular structures, increased RhoA, ROCK mRNA, and their proteins, and prominently increased CCND1, Myc, and VEGF proteins, and prominently reduced TIMP-1 protein (P<0.05). Conclusion: Lidocaine can inhibit proliferation, invasion, and vasculogenic mimicry of EC cells by suppressing RhoA/ROCK pathway. 2025 Vol. 31 (9): 1446-1452 [Abstract] ( 146 ) HTML (1 KB)  PDF (1668 KB)  ( 8 ) 1452 Effects of LncRNA SNHG7 on Migration and Invasion of Prostate Cancer Cells through Targeted Regulation of miR-146b-5p TIAN Li, et al DOI: 10.3969/j.issn.1006-6233.2025.09.08 Objective: To investigate the effects of long non-coding RNA (LncRNA) small nucleolar RNA host gene 7 (SNHG7) on migration and invasion of prostate cancer (PCa) cells through targeted regulation of microRNA-146b-5p (miR-146b-5p). Methods: QRT-PCR was used to measure the expression of LncRNA SNHG7 and miR-146b-5p in cancer tissues and adjacent tissues of 42 PCa patients who underwent PCa surgery in our hospital from August 2023 to April 2024. The logarithmic phase DU145 cells were grouped into normal group, NC-shRNA group, SNHG7-shRNA group, SNHG7-shRNA+anti-NC group, and SNHG7-shRNA+anti-miR-146b-5p group. MTT assay, scratch assay, and Transwell assay were used to detect the proliferation, migration, and invasion of DU145 cells, respectively. In addition, Western blot was used to detect the expression of epithelial mesenchymal transition (E-cadherin, Vimentin) proteins in DU145 cells. Results: The expression of LncRNA SNHG7 in PCa tissue was higher than that in adjacent tissues (t=30.622, P<0.001), and the expression of miR-146b-5p was lower than that in adjacent tissues (t=26.869, P<0.001). The A490 value, scratch healing rate, invasion number, LncRNA SNHG7, and Vimentin expression in SNHG7-shRNA group were lower than those in NC-shRNA group and Control group, while miR-146b-5p and E-cadherin were higher than those in NC-shRNA group and Control group (P<0.05). The A490 value, scratch healing rate, invasion number, LncRNA SNHG7, and Vimentin expression in SNHG7-shRNA+anti-miR-146b-5p group were higher than those in SNHG7-shRNA group and SNHG7-shRNA+anti-NC group, while miR-146b-5p and E-cadherin were lower (P<0.05). LncRNA SNHG7 could target negative regulation of miR-146b-5p. Conclusion: Silencing LncRNA SNHG7 can inhibit the migration and invasion of PCa cells, and it may be achieved by targeting negative regulation of miR-146b-5p. 2025 Vol. 31 (9): 1452-1458 [Abstract] ( 140 ) HTML (1 KB)  PDF (1747 KB)  ( 6 ) 1458 Effects of Alpinetin on the Proliferation and Invasion of Prostate Cancer Cells by Regulating LncRNA FTX/miR-335-5p Axis LEI Xiao, BI Genwang, JIA Lan, et al DOI: 10.3969/j.issn.1006-6233.2025.09.09 Objective: To explore the effects of alpinetin (ALP) on the proliferation and invasion of prostate cancer (PC) cells by regulating LncRNA FTX/miR-335-5p axis. Methods: The human prostate cancer cell line LNCaP was cultured in vitro, and the MTT colorimetric method was used to detect the proliferative activity of ALP on LNCaP cells. LNCaP cells were assigned into NC group, ALP group, ALP+pcDNA3.1-NC group, and ALP+pcDNA3.1-FTX group. CCK-8 and clone formation experiments were used to detect the proliferation ability of LNCaP cells in each group. Transwell method was used to detect the invasion ability of LNCaP cells in each group. qRT-PCR was used to detect LncRNA FTX and miR-335-5p of LNCaP cells in various groups. Western blot experiment was performed to detect cell proliferation and invasion related proteins. Dual luciferase reporter gene assay was performed to detect the targeting relationship between LncRNA FTX and miR-335-5p. Construct a nude mouse PC transplant tumor model, detect the mass and volume of tumors in each group, and measure the expression levels of LncRNA FTX and miR-335-5p in tumor tissues of each group. Results: For the NC group, the ALP group and ALP+pcDNA3.1-NC group had conspicuously lower LNCaP cell survival rate, clone formation number, cell invasion number, LncRNA FTX, cyclin D1 (CCND1), myelocytomatosis viral oncogene homolog (MYC), matrix metalloproteinase-2 (MMP) -2 and MMP-9 mRNAs and proteins (P<0.05), and conspicuously higher miR-335-5p (P<0.05). For the ALP+pcDNA3.1-NC group, the ALP+pcDNA3.1-FTX group had conspicuously higher LNCaP cell survival rate, clone formation number, cell invasion number, LncRNA FTX, cyclin D1 (CCND1), myelocytomatosis viral oncogene homolog (MYC), matrix metalloproteinase-2 (MMP) -2 and MMP-9 mRNAs and proteins (P<0.05), and conspicuously lower miR-335-5p (P<0.05). The dual luciferase reporter gene experiment showed that LncRNA FTX targeted negative regulation of miR-335-5p (P<0.05). The results of the nude mouse tumorigenesis experiment showed that compared with the Model group, the ALP group had significantly smaller tumor mass and volume, and the expression of LncRNA FTX in tumor tissue was significantly downregulated, while the expression of miR-335-5p was significantly up-regulated (P<0.05). Conclusion: ALP may inhibit proliferation and invasion of PC cells by regulating LncRNA FTX/miR-335-5p axis. 2025 Vol. 31 (9): 1458-1465 [Abstract] ( 131 ) HTML (1 KB)  PDF (1757 KB)  ( 12 ) 1465 Effects of Dexmedetomidine on the Proliferation Migration and Invasion of Ovarian Cancer Cells by Regulating Notch/JAG1 Pathway GE Xin, YUAN Lei, QIN Jiao DOI: 10.3969/j.issn.1006-6233.2025.09.010 Objective: To explore the effects of dexmedetomidine on the proliferation, migration, and invasion of ovarian cancer cells by regulating Notch/JAG1 pathway. Methods: SKOV-3 cells were grouped into control group, low-dose dexmedetomidine group (1 nmoL/L), medium-dose dexmedetomidine group (10nmoL/L), a high-dose dexmedetomidine group (100nmoL/L), and high-dose dexmedetomidine+activator group (100nmol/L dexmedetomidine+8 mmoL/L propionic acid). The proliferation ability of cells was determined using MTT assay and Edu staining method. The apoptosis of cells was determined using TUNEL staining method. The migration ability of cells was determined using the cell scratch assay. The invasion ability of cells was determined using the Transwell method. The Ki67, Cyclin D1, Bcl-2, Notch1, and JAG1 proteins in each group were determined by Western blot. Results: Compared with the control group, the low, medium, and high dose dexmedetomidine groups showed a decrease in cell OD value, Edu positive cell rate, scratch migration rate, a reduction in cell invasion number, a clear decrease in Ki67, Cyclin D1, Bcl-2, Notch1, and JAG1 proteins, and a clear increase in cell apoptosis rate and Bax protein (P<0.05), which were dose-dependent (P<0.05). Compared with the high-dose dexmedetomidine group, the high-dose dexmedetomidine+activator group showed an increase in cell OD value, Edu positive cell rate, and scratch migration rate, an increase in cell invasion number, an increase in Ki67, Cyclin D1, Bcl-2, Notch1, and JAG1 proteins, and a decrease in cell apoptosis rate and Bax protein (P<0.05). Conclusion: Dexmedetomidine inhibits ovarian cancer cell proliferation, migration, and invasion by suppressing Notch/JAG1 pathway. 2025 Vol. 31 (9): 1465-1471 [Abstract] ( 127 ) HTML (1 KB)  PDF (2111 KB)  ( 5 ) 1471 MiR-7 miR-21 Levels and Their Correlation with Proliferation Metastasis and Angiogenesis in Renal Clear Cell Carcinoma JIAO Shoukuo, ZHU Zhenyan, DENG Haibo, et al DOI: 10.3969/j.issn.1006-6233.2025.09.011 Objective: To analyze the relationship between the expression levels of microRNA 7 (miR-7), miR-21 and the proliferation, metastasis and angiogenesis of renal clear cell carcinoma (ccRCC). Methods: Collect ccRCC tissue and para-cancer tissue, culture ccRCC cell lines 786-O, RCC23, ACHN, SN12-PM6, A498 and human renal proximal tubule cell line HK-2 in vitro, and detect the expression level of miR-7 and miR-21 by qRT-PCR. A498 cell line was selected for miR-7 overexpression treatment (miR-7-mimics NC group, miR-7-mimics group), and 786-O cell line was selected for miR-21 knockdown treatment (miR-21-inhibitorsNC group, miR-21-inhibitorsgroup). The expression levels of epithelial-mesenchymal transition-related proteins were detected by Western blot, cell proliferation activity was detected by EDU proliferation assay, cell migration activity was detected by scratch assay, and cell invasion activity was detected by Transwell assay. The supernatants of the above groups of cells were co-cultured with human umbilical vein endothelial cells (HUVEC) respectively (miR-7-mimicsNC supernatant group, miR-7-mimics supernatant group, miR-21-inhibitorsNC supernatant group, miR-21-inhibitors supernatant group), and the differences in the angiogenic ability of HUVEC cells in each group were observed. Results: Compared with para-cancerous tissue, miR-7 levels were decreased and miR-21 levels were increased in ccRCC cancer tissue. Compared with HK-2 cells, miR-7 levels were decreased and miR-21 levels were increased in ccRCC cell lines. Compared with the miR-7-mimicsNC group, the protein expression levels of Vimentin and N-cadherin in the miR-7-mimics group were decreased, and the protein levels of E-cadherin and ZO-1 were increased, and the cell proliferation, migration and invasion activities were all decreased. Compared with the miR-21-inhibitorsNC group, the protein expression levels of Vimentin and N-cadherin in the miR-21-inhibitors group were decreased, the protein levels of E-cadherin and ZO-1 were increased, and the cell proliferation, migration and invasion activities were all decreased. Compared with the miR-7-mimicsNC supernatant group, the miR-7-mimics supernatant group had weaker angiogenic ability of HUVEC cells; compared with the miR-21-inhibitorsNC supernatant group, the miR-21-inhibitors supernatant group had weaker angiogenic ability of HUVEC cells. Conclusion: miR-7 was down-regulated and miR-21 was up-regulated in ccRCC. Overexpression of miR-7 or knockdown of miR-21 could inhibit the proliferation, metastasis and angiogenesis of ccRCC cells. 2025 Vol. 31 (9): 1471-1475 [Abstract] ( 143 ) HTML (1 KB)  PDF (2867 KB)  ( 7 ) 1476 Molecular Mechanisms of LncRNA FAM225A/miR-205-5p/BRCA1 Mediated DNA Damage Repair in Cervical Cancer Radiotherapy Resistance ZHEN Yulan, CHEN Yongfa, GUO Jingwen, et al DOI: 10.3969/j.issn.1006-6233.2025.09.012 Objective: To investigate the effects of LncRNA FAM225A/miR-205-5p/BRCA1 axis on DNA repair, cell proliferation, and cell apoptosis rate of radiation resistant cells of human cervical cancer (CC). Methods: Cell Experiment Grouping 1: control group (HeLa cells) and radiation-resistant group (HeLa-229 cells). Bioinformatics analysis was performed to predict the direct sequence interactions between LncRNA FAM225A and miR-205-5p, as well as between miR-205-5p and the 3'-UTR of BRCA1. Dual-luciferase reporter gene assay was used to confirm the above direct sequence interactions. Cell Experiment Grouping 2 included: ① Control Group 2 (radiation-resistant HeLa-229 cells), ② Radiation group (radiation-resistant HeLa-229 cells irradiated with 6 Gy X-rays), ③ Radiation + Aden-LncRNA FAM225A shRNA group, ④ Radiation + Aden-shRNA NC group, ⑤ Radiation + Aden-LncRNA FAM225A-OE group, ⑥ Radiation + Aden-OE vector group. Comet assay was used to determine the repair of DNA repair in cells of each group. CCK-8 assay was used to measure the cell proliferation ability of each group. Flow cytometry was used to determine the apoptosis rate. RT-qPCR was used to detect the effect of knockdown/overexpression of LncRNA FAM225A on the expression of genes related to cell apoptosis, cell cycle, DNA damage repair, and cell survival promotion. Western blot was used to determine the protein expression levels of BRCA1, BRCA2, CDK1, p-PI3K, PI3K, p-AKT, and AKT in cells of each group. Results: Compared with the control group (HeLa cells), the relative expression levels of LncRNA FAM225A and BRCA1 mRNA in the radiation-resistant group (HeLa-229 cells) were upregulated (P<0.05), while the relative expression level of miR-205-5p was downregulated (P<0.05). LncRNA FAM225A inhibited the expression of miR-205-5p through direct sequence interaction, and miR-205-5p inhibited the expression of BRCA1 through direct sequence interaction with the 3'-UTR of BRCA1. Compared with Control Group 2, which had fewer and shorter comet tails, the radiation group had longer and more severe comet tails; the radiation + Aden-LncRNA FAM225A shRNA group had more severe comet tails, with the comet head and tail separated, making it difficult to interpret; the radiation + Aden-LncRNA FAM225A-OE group had fewer and shorter comet tails compared with the radiation group. Compared with Control Group 2, the apoptosis rate of the radiation group increased (P<0.05). Compared with the radiation group, the cell proliferation ability of the radiation+Aden-LncRNA FAM225A shRNA group decreased and the apoptosis rate increased (P<0.05); the cell proliferation ability of the radiation+Aden-LncRNA FAM225A-OE group enhanced and the apoptosis rate decreased (P<0.05). The results of mRNA level determination showed that compared with Control Group 2, the relative expression levels of apoptosis-related genes (Caspase-3, BAX, FADD), cell survival-promoting genes (PARP, SOX2, CKS1B, MEIS2), and DNA damage repair-related genes (BRCA1, BRCA2, ATM, RAD51) in the radiation group were increased (P<0.05); the relative expression levels of tumor suppressor transcription factors, proto-oncogenes (SPT6, p53, RNAP II, Rb), apoptosis-related genes (BCL2, BAD), cyclin-encoding genes (CDK1, CCNE1, CCNB1, CDC25A), and oncogenic signaling pathway protein factor-encoding genes (AKT, mTOR, PI3K) were decreased (P<0.05). Compared with the radiation group, the relative expression levels of PARP, CKS1B, Caspase-3, BAX, and FADD in the radiation+Aden-LncRNA FAM225A shRNA group were increased (P<0.05); the relative expression levels of SOX2, BCL2, BAD, SPT6, p53, RNAP II, Rb, BRCA1, BRCA2, ATM, CDK1, CCNE1, CCNB1, CDC25A, AKT, mTOR, and PI3K were decreased (P<0.05). In the radiation+Aden-LncRNA FAM225A-OE group, the relative expression levels of PARP, CKS1B, Caspase-3, BAX, and FADD were decreased (P<0.05); the relative expression levels of SOX2, BCL2, BAD, SPT6, p53, RNAP II, Rb, BRCA1, BRCA2, ATM, CDK1, CCNE1, CCNB1, CDC25A, AKT, mTOR, and PI3K were increased (P<0.05). The results of protein level determination showed that compared with Control Group 2, the relative expression level of BRCA2 in the radiation group was upregulated (P<0.05), while the relative expression levels of CDK1, p-PI3K, PI3K, p-AKT, and AKT were downregulated (P<0.05). Compared with the radiation group, the relative expression levels of BRCA2, CDK1, p-PI3K, PI3K, p-AKT, and AKT in the radiation+Aden-LncRNA FAM225A shRNA group were decreased (P<0.05), while those in the radiation+Aden-LncRNA FAM225A-OE group were upregulated (P<0.05).Conclusion: LncRNA FAM225A/miR-205-5p/BRCA1 axis promoted cervical cancer radiotherapy resistance by enhancing DNA damage repair. 2025 Vol. 31 (9): 1476-1487 [Abstract] ( 133 ) HTML (1 KB)  PDF (2323 KB)  ( 14 ) 1487 Impacts of LncRNA NEAT1 on High Glucose Induced Apoptosis and Migration of Human Retinal Endothelial Cells through Targeted Regulation of Mir-125b-5p CAI Chenghang, ZHANG Jin DOI: 10.3969/j.issn.1006-6233.2025.09.013 Objective: To investigate the impacts of long non-coding RNA (LncRNA) nuclear-enriched abundant transeript 1 (NEAT1) on high glucose (HG) induced apoptosis and migration of human retinal endothelial cells (RMECs) through targeted regulation of microRNA-125b-5p (miR-125b-5p). Methods: RMECs were assigned into Control group (normal culture), HG group (30mmol/L glucose induction for 24 hours), sh-NC group (HG+transfection of sh-NC), sh-NEAT1 group (HG+transfection of sh-NEAT1), sh-NEAT1+inhibitor-NC group (HG+transfection of sh-NEAT1+inhibitor-NC), and sh-NEAT1+miR-125b-5p inhibitor group (HG+transfection of sh-NEAT1+miR-125b-5p inhibitor). QRT-PCR was used to detect the expression of LncRNA NEAT1 and miR-125b-5p of RMECs in each group. MTT assay, scratch assay, and flow cytometry were used to detect the proliferation, migration, and apoptosis of RMECs, respectively. ELISA method was used to detect the expression of TNF - α, IL-6, and IL-8 in the supernatant of RMEC. Western blot was used to detect the expression of MMP-9 and Cleaved Caspase-3 proteins in RMECs. Results: The LncRNA NEAT1, apoptosis rate, TNF - α, IL-6, IL-8, and Cleaved Caspase-3 in HG group were higher than those in Control group, while miR-125b-5p, A490 value, scratch healing rate, and MMP-9 were lower (P<0.05). The LncRNA NEAT1, apoptosis rate, TNF - α, IL-6, IL-8, and Cleaved Caspase-3 in sh-NEAT1 group were lower than those in HG and sh-NC groups, while miR-125b-5p, A490 value, scratch healing rate, and MMP-9 were higher (P<0.05). The apoptosis rate, TNF-α, IL-6, IL-8, and Cleaved Caspase-3 in sh-NEAT1+miR-125b-5p inhibitor group were higher than those in sh-NEAT1 group and sh-NEAT1+inhibitor-NC group, while miR-125b-5p, A490 value, scratch healing rate, and MMP-9 were lower (P<0.05). LncRNA NEAT1 could target negative regulation of miR-125b-5p. Conclusion: Silencing LncRNA NEAT1 can promote the proliferation and migration of RMECs, inhibit cell apoptosis, and it may be achieved by targeted regulation of miR-125b-5p. 2025 Vol. 31 (9): 1487-1493 [Abstract] ( 124 ) HTML (1 KB)  PDF (1587 KB)  ( 16 ) 1493 Effect of Bushen Huoxue Recipe on Ultrastructure of Ovary in Rats with Decreased Ovarian Reserve HAO Yanhong, LIU Yulan, GAO Hui, et al DOI: 10.3969/j.issn.1006-6233.2025.09.014 Objective: To explore the effect of the Bushen Huoxue Recipe on the ultrastructure of the ovaries in rats with Diminished Ovarian Reserve (DOR). Methods: The DOR rat model was established with intraperitoneal injection of cyclophosphamide, 50mg/kg on the first day and 4mg/kg once a day on second day to 14th day. The rats were divided into blank control group, model group, Bushen Huoxue Recipe high, medium and low dose groups, and western medicine Conjugated Estrogens Tablets as positive controls, with 10 rats in each group. The behavioral changes of the rats were observed during administration, and the ultrastructure of the ovary was observed under electron microscope after the last administration. Results: Compared with the treatment groups, the rats in the model group showed decreased activity, arched backs, curled up postures, disheveled and shedding fur, irritability, and weight loss. Under transmission electron microscope, the ovarian tissues of rats in the model group showed dilated and vacuolated rough endoplasmic reticulum, ribosomes detached from the membrane, nuclear pyknosis, widened perinuclear space, obvious mitochondrial swelling, broken and disappeared cristae, and blurred membrane fusion. In the high and medium dose groups of Bushen Huoxue Recipe and the Conjugated estrogen tablet group, the nuclei of granulosa cells in the follicles were relatively large, nuclear pyknosis was not obvious, the perinuclear space was widened in some cases, and the mitochondrial morphology and structure were basically normal or showed mild mitochondrial swelling, partial disappearance of cristae, and partial blurred membranes. In the low-dose group, dilated and vacuolated rough endoplasmic reticulum, ribosomes detached from the membrane, nuclear pyknosis, more vacuolated mitochondria, mitochondrial swelling, broken and disappeared cristae, and disrupted membrane structure were observed, and autophagosomes were visible. Conclusion: The ultrastructure of ovary in DOR rats was disturbed, the apoptosis of follicular granulosa cells was obvious, and the mitochondria were damaged. Bushen Huoxue Recipe in medium and high dose and Conjugated estrogen tablet group has obviously improved effect, and the effect of Bushen Huoxue Recipe shows a certain dose-effect relationship. 2025 Vol. 31 (9): 1493-1497 [Abstract] ( 131 ) HTML (1 KB)  PDF (2209 KB)  ( 7 ) 1497 Effect of Ligustilide on Hypoxia/Reoxygenation Induced Myocardial Cell Injury by Adjusting the Notch1/Hes1 Signaling Pathway LI Lihua, ZHANG Ning, XU Ying, et al DOI: 10.3969/j.issn.1006-6233.2025.09.015 Objective: To explore the effects of ligustilide on hypoxia/reoxygenation induced myocardial cell injury by adjusting Notch1/Hes1 signaling pathway. Methods: H9C2 cardiomyocytes were used as the research object and grouped: cells with normal culture were labeled as the normal group, hypoxia/reoxygenation induction as the hypoxia/reoxygenation group, hypoxia/reoxygenation induction+10μmoL/L ligustilide as the hypoxia/reoxygenation+low-dose ligustilide group, hypoxia/reoxygenation induction+20μmoL/L ligustilide as the hypoxia/reoxygenation+medium-dose ligustilide group, hypoxia/reoxygenation induction+40μmoL/L ligustilide as the hypoxia/reoxygenation+high-dose ligustilide group, and hypoxia/reoxygenation induction+40μmoL/L ligustilide+5 mg/L Notch activator Jagged1 as the hypoxia/reoxygenation+high-dose ligustilide+Jagged1 group. MTT, flow cytometry, ELISA, and Western blot were used to detect cell proliferation, apoptosis, LDH, CK, AST, IL-18, IL-1β levels, and the Notch1/Hes1 signaling pathway related proteins. Results: The hypoxia/reoxygenation group showed decreased OD value, and increased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the normal group (P<0.05). The hypoxia/reoxygenation+low, medium, and high-dose ligustilide groups showed increased OD value, and decreased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the hypoxia/reoxygenation group (P<0.05). The hypoxia/reoxygenation+high-dose ligustilide+Jagged1 group showed decreased OD value, and increased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the hypoxia/reoxygenation+high-dose ligustilide group. Conclusion: The improvement effect of ligustilide on hypoxia/reoxygenation induced myocardial cell injury may be achieved by inhibiting Notch1/Hes1 signaling pathway. 2025 Vol. 31 (9): 1497-1502 [Abstract] ( 110 ) HTML (1 KB)  PDF (1495 KB)  ( 10 ) 1502 Application of the Radial Dorsal Digital Artery Perforator Pedicled Propeller Flap in the Repair of Finger Skin Defects Patients and Its Effect on Serum Levels of IL-6 TNF-α and IL-8 LI Shouxi, ZHANG Liangyin, LYU Changju, et al DOI: 10.3969/j.issn.1006-6233.2025.09.016 Objective: To investigate the application effect of perforator pedicled propeller flap (PPPF) with radial dorsal digital artery in repairing skin defects of the fingers and its influence on serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-8 (IL-8). Methods: A retrospective analysis was conducted on the clinical data of 80 patients with finger skin defects admitted to The 1st People's Hospital of Baiyin from March 2020 to March 2024. According to the surgical plan, they were divided into an observation group (n=40) and a control group (n=40), and underwent radial dorsal finger artery PPPF repair and conventional radial dorsal skin flap repair, respectively. The two groups were statistically analyzed in terms of perioperative indicators, serum IL-6, TNF - α, IL-8 levels, peripheral blood circulation indicators, hand function related indicators, complications, and satisfaction with finger cosmetic procedures. Results: The observation group had a shorter wound healing time, hospitalization time, and antibacterial drug use time than the control group, and a lower two-point discrimination than the control group (P<0.05). The levels of serum IL-6, TNF-α, and IL-8 in the observation group were lower than those in the control group on postoperative days 3 and 7 (P<0.05). At 3 and 6 months post-surgery, the observation group exhibited higher tissue partial pressure of oxygen (TcPO2), blood perfusion, Michigan Hand Function Questionnaire (MHQ) scores, and total finger joint mobility compared to the control group. Additionally, the cold intolerance symptom severity (CISS) score was lower in the observation group than in the control group (P<0.05). The complication rate in the observation group was lower than that in the control group 6 months after surgery, and the cosmetic satisfaction rate of the affected fingers was higher than that in the control group (P<0.05). Conclusion: The use of the radial dorsal digital artery PPPF to repair skin defects in fingers can reduce inflammatory reactions, promote wound healing, improve blood flow in the affected finger, restore hand function, reduce the risk of complications, and improve cosmetic satisfaction in the affected finger. 2025 Vol. 31 (9): 1502-1508 [Abstract] ( 127 ) HTML (1 KB)  PDF (1524 KB)  ( 17 ) 1508 Analysis of the Correlation between Serum PAR ATF5 Smad3 with Renal Function Index Blood Lipid Levels and Prognosis in Patients with Type 2 Diabetes Nephropathy DA Chenxing, YANG Shujie, XU Mengmeng DOI: 10.3969/j.issn.1006-6233.2025.09.017 Objective: To explore the correlation between serum platelet albumin ratio (PAR), activating transcription factor 5 (ATF5), Smad3 with renal function indicators, blood lipid levels and prognosis in patients with type 2 diabetes nephropathy. Methods: From December 2019 to January 2022, 152 patients with type 2 diabetes nephropathy (diabetes nephropathy group) and 100 patients with T2DM (simple diabetes group) who visited our hospital were selected. The diabetes nephropathy group was assigned into good group (119 cases) and poor group (33 cases) complying with the prognosis. Pearson method was used to analyze the correlation between serum PAR, ATF5, Smad3 with renal function indexes, blood lipid levels in diabetes nephropathy group. Logistic method was performed to analyze the prognostic factors of type 2 diabetes nephropathy patients. ROC was performed to analyze the prognostic value of serum PAR, ATF5, Smad3 in patients with type 2 diabetes nephropathy. Results: The diabetes nephropathy group had conspicuously higher UACR and PAR, ATF5 and Smad3, and conspicuously lower eGFR than the simple diabetes group (P<0.05). The poor group had conspicuously higher serum PAR, ATF5, and Smad3 than good group (P<0.05). Serum PAR, ATF5, and Smad3 were positively correlated with UACR (r=0.423, 0.439, 0.431, P<0.05) and negatively correlated with eGFR (r=-0.408, -0.389, -0.396, P<0.05), respectively. Elevated serum PAR, ATF5 and Smad3 were risk factors for poor prognosis of type 2 diabetes nephropathy patients (P<0.05). The AUC of serum PAR, ATF5 and Smad3 in predicting the prognosis of type 2 diabetes nephropathy patients was 0.919, with a sensitivity of 84.85% and a specificity of 89.92%. The predictive value of the combination of the three for the prognosis of type 2 diabetes nephropathy patients was higher than that of the single prediction (Zcombination-PAR=2.562, P=0.010, Zcombination-ATF5=2.842, P=0.005, Zcombination-Smad3=2.781, P=0.005). Conclusion: The serum PAR, ATF5 and Smad3 in type 2 diabetes nephropathy patients are elevated, and the three levels are respectively related to renal function. The combined detection of the three has potential predictive value for prognosis of type 2 diabetes nephropathy patients. 2025 Vol. 31 (9): 1508-1514 [Abstract] ( 129 ) HTML (1 KB)  PDF (1226 KB)  ( 10 ) 1514 Expression Changes of miR-491-5p and ZNF703 in Breast Cancer Tissues and The Relationship with Prognosis ZHOU Aiping, YANG Fan, WU Landi, et al DOI: 10.3969/j.issn.1006-6233.2025.09.018 Objective: To investigate the expression changes of microRNA-491-5p (miR-491-5p) and zinc finger protein 703 (ZNF703) in breast cancer tissues and their relationship with prognosis. Methods: A total of 153 breast cancer patients (breast cancer group) who underwent surgical resection in our hospital from January 2017 to August 2019, and 153 patients with benign breast tumors who underwent benign breast lesion resection during the same period (control group), were included. Real-time quantitative polymerase chain reaction was used to detect the expression of miR-491-5p and ZNF703 mRNA in breast cancer and benign lesion tissues. Online databases were used to predict the binding sites between miR-491-5p and ZNF703, and the correlation between miR-491-5p and ZNF703 mRNA expression in breast cancer tissues was analyzed using Pearson correlation analysis. Based on the average expression levels of miR-491-5p and ZNF703 mRNA in breast cancer tissue, patients were divided into high-expression and low-expression groups. Kaplan-Meier survival curves were plotted for breast cancer patients with different miR-491-5p and ZNF703 expression levels, and multivariate Cox regression analysis was performed to examine the relationship between miR-491-5p and ZNF703 mRNA expression and patient prognosis. Results: Compared with the control group, miR-491-5p was under-expressed, while ZNF703 mRNA was over-expressed in the breast cancer group (P<0.05). A binding site was identified at the 3'-untranslated region (127-133) of ZNF703 for miR-491-5p, and the two showed a negative correlation in breast cancer tissues (P<0.05). Breast cancer tissues with a tumor diameter ≥2 cm, low differentiation, TNM stage Ⅲ-Ⅳ, lymph node metastasis, or distant metastasis showed lower miR-491-5p expression and higher ZNF703 mRNA expression compared to those with a tumor diameter <2 cm, medium/high differentiation, TNM stage Ⅰ-Ⅱ, no lymph node metastasis, or no distant metastasis (P<0.05). The 5-year overall survival rate of 153 breast cancer patients was 77.12% (118/153). Patients with high miR-491-5p expression had a higher 5-year survival rate than those with low miR-491-5p expression, while those with high ZNF703 mRNA expression had a lower survival rate compared to patients with low ZNF703 mRNA expression (P<0.05). Multivariate Cox regression analysis showed miR-491-5p ≥0.86 was identified as an independent protective factor, while ZNF703 mRNA ≥1.46 was an independent risk factor for breast cancer patient mortality (P<0.05). Conclusion: miR-491-5p is under-expressed and ZNF703 mRNA is over-expressed in breast cancer tissues, which are associated with poor pathological features and prognosis. They may serve as novel biomarkers for evaluating breast cancer prognosis. 2025 Vol. 31 (9): 1514-1520 [Abstract] ( 135 ) HTML (1 KB)  PDF (1452 KB)  ( 6 ) 1521 The Correlation between Serum Hs-CRP Hcy and MPO Levels and Coronary Atherosclerosis in Hemodialysis Patients SHI Xueting, YE Tao, LAN Kai, et al DOI: 10.3969/j.issn.1006-6233.2025.09.019 Objective: To observe serum levels of high-sensitivity C-reactive protein (hs-CRP), homocysteine (Hcy) and myeloperoxidase (MPO) in hemodialysis patients, and analyze their correlation with coronary atherosclerosis. Methods: Totally 124 hemodialysis patients admitted to the Third People's Hospital of Chengdu from January 2021 to December 2023 were selected. According to the results of coronary artery angiography, the patients were divided into coronary atherosclerosis group and control group. General information and serum hs-CRP, Hcy and MPO levels of the two groups were compared. The risk factors for coronary artery atherosclerosis in hemodialysis patients were analyzed using multiple Logistic regression, and the correlation between serum hs CRP, Hcy, MPO levels and coronary artery atherosclerosis was analyzed using Spearman correlation analysis. Results: Among 124 hemodialysis patients, 51 (41.13%) had coronary atherosclerosis, while 73 (58.87%) had no coronary atherosclerosis. In the general information of the two groups of patients, there were statistically significant differences in dialysis age, systolic blood pressurethe, the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) between the two groups (P<0.05). Compared to the control group, the coronary atherosclerosis group exhibited significantly higher levels of serum hs-CRP, Hcy, and MPO, with statistically significant differences (P<0.05). Logistic regression analysis showed that, LDL-C, HDL-C, hs-CRP, Hcy, and MPO levels were risk factors for coronary atherosclerosis in hemodialysis patients (P<0.05). Conclusion: The occurrence of coronary arteriosclerosis in hemodialysis patients is not only related to serum LDL-C and HDL-C, but also closely related to hs CRP, Hcy, and MPO levels. In clinical practice, high attention should be paid to the changes in serum LDL-C, HDL-C, hs CRP, Hcy, and MPO levels in hemodialysis patients to assess their risk of coronary arteriosclerosis as early as possible and take intervention measures. 2025 Vol. 31 (9): 1521-1525 [Abstract] ( 138 ) HTML (1 KB)  PDF (1087 KB)  ( 9 ) 1525 Analysis of Factors Influencing Sudden Cardiac Death in Elderly Patients with Dilated Cardiomyopathy Arrhythmias and Its Predictive Value LI Xueyu, CHEN Qiao, LAI Na, et al DOI: 10.3969/j.issn.1006-6233.2025.09.020 Objective: To explore the influencing factors and predictive value of sudden cardiac death (SCD) in elderly patients with arrhythmogenic dilated cardiomyopathy (AR-DCM). Methods: A total of AR-DCM patients diagnosed at our hospital from January 2022 to January 2025 were selected as the subjects of this study, and divided into non-SCD group (n=142) and SCD group (n=52) based on whether SCD occurred. Univariate analysis was performed on patients' clinical data, tests, and electrocardiographic examination data; Spearman correlation analysis was used to assess the correlation between each indicator and the occurrence of SCD; multivariate binary Logistic regression analysis was conducted to identify the influencing factors of SCD in elderly AR-DCM patients; and ROC curve analysis was used to evaluate the predictive value of the relevant indicators. Results: The proportion of NYHA Class Ⅲ and Ⅳ in elderly AR-DCM patients with SCD, as well as the levels of cTnT and NT-proBNP, and the duration of Tp-Te and Tc were higher in patients without SCD (P<0.05), but only the level of NT-proBNP was an independent influencing factor (P<0.05), and had a high predictive value (AUC=0.965). Conclusion: The level of NT-proBNP is an independent influencing factor for the occurrence of SCD and has the best predictive value. 2025 Vol. 31 (9): 1525-1531 [Abstract] ( 140 ) HTML (1 KB)  PDF (1286 KB)  ( 10 ) 1531 Effect of Endoscopy Combined with Early Somatostatin or Terlipressin Treatment for Esophagogastric Variceal Bleeding in Liver Cirrhosis on Liver and Kidney Function WANG Xiaoxia, ZHANG Huiyue, ZUO Luo, et al DOI: 10.3969/j.issn.1006-6233.2025.09.021 Objective: To investigate the effect of endoscopy combined with early somatostatin or terlipressin treatment for esophagogastric variceal bleeding (EGVB) in liver cirrhosis on liver and kidney function. Methods: From March 2021 to March 2024, 90 patients with liver cirrhosis and EGVB were selected. Using the random number table method, they were assigned to group A (45 cases) and group B (45 cases), receiving terlipressin treatment and somatostatin treatment, respectively. Both groups underwent endoscopic ligation after 48h of treatment. The two groups were compared on hemostasis, blood routine, liver function, renal function, and adverse reactions. Results: There were no statistically significant differences in hemostasis time, early rebleeding rate, delayed rebleeding rate, differences in blood routine indices (hemoglobin, albumin, white blood cell count, red blood cell count and platelet count) before and after treatment, and overall incidence of adverse reactions between the two groups (P>0.05). After treatment, the levels of alanine aminotransferase (ALT), total bilirubin (TBIL), serum creatinine (Scr), blood urea nitrogen (BUN), aspartate aminotransferase (AST), cystatin C (Cys C) and uric acid (UA) in both groups decreased. Besides, the decreases were greater in group A (P<0.05). The creatinine clearance rate (CCR) increased in both groups, and the increase was greater in group A (P<0.05). Conclusion: Endoscopy combined with early somatostatin and endoscopy combined with terlipressin can achieve similar hemostatic effects in patients with liver cirrhosis and EGVB. However, the latter can protect the patients' liver and kidney function. 2025 Vol. 31 (9): 1531-1535 [Abstract] ( 151 ) HTML (1 KB)  PDF (1093 KB)  ( 5 ) 1536 Correlation and Predictive Significance of MELD Score I-FABP MDH1 and the Disease Outcome of Patients with End-Stage Liver Disease Complicated with CRE Spontaneous Peritonitis XIAO Ying, ZHANG Jingjing, ZHANG Jiandong, et al DOI: 10.3969/j.issn.1006-6233.2025.09.022 Objective: To investigate the correlation between the Model for End-stage Liver Disease (MELD) score, intestinal fatty acid binding protein (I-FABP), and malate dehydrogenase 1 (MDH1) in patients with end-stage liver disease (ESLD) complicated with carbapenem-resistant Enterobacteriaceae (CRE) spontaneous peritonitis (SBP), and to analyze their predictive value for disease outcome. Methods: A total of 205 patients with end-stage liver disease complicated with CRE SBP admitted to our hospital from July 2021 to July 2024 were selected. Based on the 28-day disease progression, they were categorized into poor outcome group and good outcome group.. Baseline data, MELD score, I-FABP, and MDH1 were compared between the two groups. The independent factors related to the disease outcome was analyzed, and four models were established: Model 1 for uncorrected hepatocellular carcinoma and refractory ascites, Model 2 for uncorrected hepatocellular carcinoma, refractory ascites, hepatic encephalopathy, and ascitic white blood cells, Model 3 for uncorrected hepatocellular carcinoma, refractory ascites, hepatic encephalopathy, ascitic white blood cells, and ascitic neutrophils, and Model 4 for corrected hepatocellular carcinoma, refractory ascites, hepatic encephalopathy, ascitic white blood cells, and ascitic neutrophils. According to the MELD score, I-FABP, and MDH1 quartile grouping, the consistency with the COX proportional hazards regression model was analyzed. Smooth curve fitting was used to analyze the correlation between MELD score, I-FABP, MDH1, and disease outcome. Based on model 4, a nomogram prediction model was established to analyze the predictive value of the model for disease outcomes and to validate it. Results: The proportions of patients with hepatocellular carcinoma, refractory ascites, and hepatic encephalopathy, as well as the levels of white blood cells and neutrophils in ascites, were significantly higher in the poor outcome group than in the good outcome group (P<0.05). The MELD score, I-FABP, and MDH1 in the poor group were significantly higher than those in the good group (P<0.05). After adjusting for hepatocellular carcinoma, refractory ascites, hepatic encephalopathy, ascitic white blood cells, and ascitic neutrophils, MELD score, I-FABP, and MDH1 remained independent factors associated with disease outcome (P<0.05). After stratifying the MELD score, I-FABP, and MDH1, a sensitivity analysis was conducted on model 4. The results of the stratified analysis were consistent with the aforementioned COX proportional hazards regression model, suggesting that the research results were relatively stable. Smooth curve fitting showed that MELD score, I-FABP, and MDH1 had a positive linear relationship with disease outcome (P<0.05). The AUCs of models 1, 2, 3, and 4 for predicting disease outcomes were 0.717, 0.749, 0.861, and 0.942, respectively, and the AUC of model 4 was significantly greater than that of models 1, 2, and 3 (P<0.05). The nomogram analysis showed that the C-index of model 4 was 0.898, and the nomogram model had good discrimination and predictive ability. This nomogram model had good clinical utility in predicting disease outcomes. Conclusion: Patients with end-stage liver disease complicated with CRE spontaneous peritonitis who have a poor prognosis have an increased MELD score and serum I-FABP and MDH1 levels, which are independent factors associated with poor prognosis and have a positive linear relationship with prognosis. Based on the MELD score, I-FABP, and MDH1, a nomogram model is established, which has good predictive performance and clinical utility for predicting the disease outcome. 2025 Vol. 31 (9): 1536-1543 [Abstract] ( 134 ) HTML (1 KB)  PDF (1569 KB)  ( 116 ) 1543 The Correlation of Serum IL-10 and Serum sPD-1 with the Severity and Prognosis of Psoriasis GAO Yue, FAN Leiqiang DOI: 10.3969/j.issn.1006-6233.2025.09.023 Objective: To investigate the correlation of serum IL-10 and serum sPD-1 with the severity and prognosis of psoriasis. Methods: Totally 150 psoriasis patients who received treatment in Chengde Central Hospital from March 2021 to February 2024 were selected as the research subjects and included in the study group. According to the scoring results of Psoriasis Skin Area and Severity Index (PASI), the study group was further divided into a mild group (81 cases) and a moderate to severe group (69 cases). Another 75 healthy individuals who underwent physical examinations during the same period were selected as the control group. Serum IL-10 and sPD-1 levels were compared between the study group and the control group; PASI scores, serum IL-10 level, and sPD-1 level were compared between the mild and moderate to severe groups. The correlation of serum IL-10 and sPD-1 levels with the severity of psoriasis was analyzed. PASI scores, serum IL-10 level, and sPD-1 level of psoriasis patients were compared before and after treatment. Results: Compared with the control group, the study group had lower serum IL-10 level and higher serum sPD-1 level, with significant statistical difference (P<0.05). The PASI score and serum sPD-1 level in the mild group were lower, while serum IL-10 level was higher, than those in the moderate to severe group, and the differences were statistically significant (P<0.05). The severity of psoriasis is negatively correlated with serum IL-10 level and positively correlated with serum sPD-1 levels (P<0.05). Before treatment, after 2 weeks treatment, and after 4 weeks treatment, PASI score and serum sPD-1 level of psoriasis patients decreased sequentially, while serum IL-10 level increased sequentially, with significant statistical differences (P<0.05). Conclusion: Serum IL-10 level is negatively correlated with the severity and prognosis of psoriasis patients, while serum sPD-1 level is positively correlated. Both serum IL-10 and sPD-1 levels can serve as important reference indicators for the clinical diagnosis and treatment of psoriasis. 2025 Vol. 31 (9): 1543-1547 [Abstract] ( 147 ) HTML (1 KB)  PDF (1109 KB)  ( 11 ) 1547 Comparison of Umbilical Incision Laparoscopic Assisted Hernia Hook Needle and Lower Abdominal Transverse Small Incision Surgery in Inguinal Hernia in Children MA Xiaopeng, CHEN Shan, XUE Yadong, et al DOI: 10.3969/j.issn.1006-6233.2025.09.024 Objective: To compare the application effects and complications of lower abdominal transverse small incision surgery or umbilical incision laparoscopic assisted hernia hook needle surgery for inguinal hernia in children. Methods: The medical records of 120 children with inguinal hernia in the hospital from November 2019 to November 2023 were retrospectively analyzed. According to different treatment methods, 62 children who received laparoscopic assisted hernia hook needle surgery were included in group A, and 58 children who adopted lower abdominal transverse small incision surgery were set as group B. The surgical conditions, postoperative pain degree [face, legs, activity, cry, consolability behavioral tool (FLACC)] and postoperative recovery process in the two groups were observed. The body stress [serum cortisol (Cor), superoxide dismutase (SOD), β-endorphin (EP)] and femoral venous hemodynamic changes before and after surgery were compared, and the postoperative short-term and long-term complications were observed in both groups. Results: There was no statistical difference in surgical time between groups (P>0.05). Compared with group B, the intraoperative blood loss, total length of surgical incision, spontaneous activity time, postoperative gastrointestinal function recovery time and hospitalization time in group A were less or shorter (P<0.05). The FLACC scores in group A at 12, 24 and 48 hours after surgery were lower compared with those in group B (P<0.05). No statistical differences were observed in serum Cor level, SOD level, β-EP level, femoral vein diameter, blood flow velocity and blood flow between groups before surgery (P>0.05). The levels of serum Cor, SOD and β-EP in both groups at 1 day after surgery were increased compared with those before surgery (P<0.05), and the increases of the above levels in group A were lower than those in group B (P<0.05). The femoral vein diameter, blood flow velocity and blood flow volume were increased in both groups at 7 days after surgery compared to before surgery (P<0.05), and the increases of the above indexes were higher in group A than those in group B (P<0.05). The total incidence rate of postoperative short-term complications and total incidence rate of long-term complications were not statistically different between groups (P>0.05). Conclusion: Both surgical methods are safe and feasible for the treatment of inguinal hernia in children. Compared with lower abdominal transverse small incision surgery, umbilical incision laparoscopic assisted hernia hook needle has the advantages of smaller surgical trauma, faster postoperative recovery and milder body's stress degree, and it has fewer postoperative complications. 2025 Vol. 31 (9): 1547-1553 [Abstract] ( 132 ) HTML (1 KB)  PDF (1129 KB)  ( 8 ) 1553 Clinical Efficacy and Safety of Cold Loop Resection and Endoscopic Mucosal Resection for the Treatment of Colonic Polyps ZHANG Zhe, et al DOI: 10.3969/j.issn.1006-6233.2025.09.025 Objective: To compare the clinical efficacy and safety of cold loop resection (CSP) and endoscopic mucosal resection (EMR) for the treatment of colonic polyps. Methods: A total of 103 patients with colon polyps who visited the hospital from January 2023 to January 2024 were included in the study. They were grouped by surgical method, including 48 patients in the CSP group and 55 patients in the EMR group. The levels of inflammatory factors (IL-6, IL-1, TNF-α) in the two groups were observed, and the relevant indicators of surgery (operation time, hospital stay, completion of each operation, and number of complications) were statistically analyzed. Results: There was a statistically significant difference in the levels of two inflammatory factors, IL-6 and IL-1 (P<0.05), while there was no statistically significant difference in TNF-α (P>0.05). Regarding the surgical-related indicators, there was no statistically significant difference in the success rate of ileocecal tube insertion, the complete recovery rate of polyps, the rate of complete resection of polyps, and the rate of complete histological resection (P>0.05). There was also statistically significant difference in the duration of surgery and hospital stay, as well as the total incidence of complications (P<0.05). Conclusion: CSP has less inflammation, shorter procedure time, shorter hospitalization, fewer complications, higher probability of non-recurrence of polyps, and more advantageous safety and clinical efficacy in the treatment of colonic polyps. However, EMR has its application value for specific larger polyps, and the clinical situation should be based on the patient's reasonable choice of operation. 2025 Vol. 31 (9): 1553-1557 [Abstract] ( 135 ) HTML (1 KB)  PDF (1098 KB)  ( 15 ) 1557 The Expression and Clinical Significance of AAT NLRP3 and Gal3 in the Serum of Patients with Allergic Rhinitis WANG Yumei, WANG Shengjun, LI Guosheng DOI: 10.3969/j.issn.1006-6233.2025.09.026 Objective: To investigate the expression and clinical significance of α-antitrypsin (AAT), nucleotide oligomerization domain-containing protein 3 (NLRP3), and galectin-3 (Gal-3) in the serum of patients with allergic rhinitis (AR). Methods: A total of 152 AR patients admitted to our hospital from March 2020 to March 2023 were included as the study group. They were grouped into mild group and moderate to severe group based on the severity of disease. Additionally, 152 healthy individuals who underwent physical examinations were selected as the control group. ELISA was applied to detect the levels of AAT, NLRP3, Gal3, interferon gamma (INF-γ), and interleukin-4 (IL). Flow cytometry was used to detect type I T helper cells (Th1) and type II T helper cells (Th2). Pearson and Spearman correlations were applied to analyze the correlation between serum AAT, NLRP3, and Gal3 with Th1, Th2 and their cytokines, SFAR, and VAS scores. The influencing factors of moderate to severe AR patients were analyzed using multivariate Logistic regression. ROC curve was plotted to analyze the diagnostic value of serum AAT, NLRP3, and Gal3 in patients with moderate to severe AR. Results: The serum levels of AAT, NLRP3 and Gal3, Th2 and IL-4 in the study group were significantly higher than those in the control group, and the levels of Th1, Th1/Th2 and IFN-γ were significantly lower than those in the control group, and the differences were statistically significant (P<0.05). The serum AAT, NLRP3 and Gal3 levels, Th2, IL-4, SFAR and VAS scores in the moderate to severe group were significantly higher than those in the mild group, and the differences in Th1, Th1/Th2 and IFN-γ were significantly lower than those in the mild group (P<0.05). According to Pearson correlation, serum AAT, NLRP3, and Gal3 were all associated with Th1, Th2, Th1/Th2, IFN-γ, and IL-4 (P<0.05). According to Spearman correlation, serum AAT, NLRP3, and Gal3 were all related to SFAR and VAS scores (P<0.05). Multivariate Logistic regression analysis showed that AAT, NLRP3, Gal3, Th2, and IL-4 were risk factors affecting patients with moderate to severe AR (P<0.05), while Th1, Th1/Th2, and IFN-γ were protective factors (P<0.05). According to the ROC curve, the AUC of serum AAT, NLRP3, and Gal3 in diagnosing moderate to severe AR patients was 0.825, 0.842, and 0.828, respectively, the AUC of the three combination in diagnosing moderate to severe AR patients was 0.943, and their combined diagnosis was superior to their individual diagnoses (Z=2.689, Z=2.702, Z=2.712, all P<0.05). Conclusion: The levels of AAT, NLRP3, and Gal3 in the serum of AR patients are all elevated, which are related to the severity of the disease. Combined detection can effectively improve the diagnostic value for moderate to severe AR. 2025 Vol. 31 (9): 1557-1563 [Abstract] ( 144 ) HTML (1 KB)  PDF (1281 KB)  ( 11 ) 1563 Application of MALDI-TOF MS and TB-SAT Combined Detection in the Diagnosis of Non-Tuberculous Mycobacterial Diseases REN Zhe, FENG Jianchun, XIE Jiong, et al DOI: 10.3969/j.issn.1006-6233.2025.09.027 Objective: To explore the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with tuberclebacillus- simultaneous amplification and testing (TB-SAT) in the diagnosis of non-tuberculous mycobacteria (NTM) pulmonary disease. Methods: A total of 152 suspected NTM lung disease patients admitted to Hebei Provincial Chest Hospital from January 2020 to December 2024 were selected for MALDI-TOF MS and TB-SAT examination of bronchoalveolar lavage fluid (BALF) specimens. The diagnostic criteria for NTM lung disease were used as the gold standard to analyze the efficacy of MALDI-TOF MS and TB-SAT in screening NTM lung disease and their consistency with the gold standard. The efficacy of MALDI-TOF MS and TB-SAT in jointly diagnosing NTM lung disease and the distribution of NTM lung bacteria species was analyzed. Results: Based on the diagnostic criteria for NTM lung disease, 80 cases (53.63%) were finally diagnosed, with 46.51% primarily involving Mycobacterium intracellulare; the positive rates of MALDI-TOF MS and TB-SAT for detecting NTM pulmonary disease were 81.25% and 78.75%, respectively. The Kappa values of the two detection techniques for consistency with the gold standard were 0.533 (95%CI: 0.376-0.691) and 0.538 (95%CI: 0.378-0.697), respectively. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of MALDI-TOF MS and TB-SAT combined diagnosis were higher than those of single Screening (P<0.05). The consistency of the combined diagnosis of NTM pulmonary pathogen distribution and gold standard by MALDI-TOF MS and TB-SAT was 0.882 (95%CI: 0.757-1.007). Conclusion: The combined detection of MALDI-TOF MS and TB-SAT has good diagnostic value in NTM lung disease, which can improve diagnostic accuracy and provide strong support for accurate clinical diagnosis of NTM. 2025 Vol. 31 (9): 1563-1568 [Abstract] ( 133 ) HTML (1 KB)  PDF (1320 KB)  ( 5 ) 1568 Relationship of Red Blood Cell Distribution Width and Hematocrit Level with qSOFA Score in Elderly Patients with Upper Gastrointestinal Bleeding and Evaluation Value on Prognosis WANG Qiuyue, XIE Faping, ZHOU Renzheng, et al DOI: 10.3969/j.issn.1006-6233.2025.09.028 Objective: To observe the changes of red blood cell distribution width (RDW) and hematocrit (Hct) in elderly patients with upper gastrointestinal bleeding (UGB), and to analyze the relationship with quick sequential organ failure assessment (qSOFA) score and the predictive value on prognosis of patients. Methods: Totally 104 elderly patients with UGB in the hospital from December 2021 to May 2024 were included. The levels of RDW and Hct in the blood of patients were measured, and the association between changes of the two indexes and qSOFA score and prognosis of patients was observed. Results: The RDW level, qSOFA score, age and proportion of bleeding volume≥1000 mL in elderly UGB patients with poor prognosis were higher than those in patients with good prognosis while the Hct was lower than that in patients with good prognosis, with statistical differences (P<0.05). Logistic regression analysis showed that RDW, qSOFA score and blood loss volume≥1000 mL are the risk factors affecting the poor prognosis of elderly UGB patients, and Hct was a protective factor, and the associations were statistically significant (P<0.05). Spearman correlation analysis indicated that RDW level in elderly UGB patients was positively correlated with qSOFA score, and Hct was negatively correlated with qSOFA score, with statistical correlations (r=0.207, -0.197, P<0.05). ROC curve demonstrated that the AUCs of RDW and Hct in evaluating the poor prognosis of elderly UGB patients were 0.731 and 0.777, respectively, while the AUC of RDW+Hct in assessing the poor prognosis was 0.845, higher than AUC of RDW and Hct, and the difference was statistically significant (P<0.05). The sensitivity and specificity under the maximum Youden index were 0.724 and 0.827, respectively. Conclusion: The increase of RDW and decrease of Hct in elderly UGB patients are related to the qSOFA score and prognosis of patients, and the levels of RDW and Hct have certain evaluated value on the poor prognosis of patients. 2025 Vol. 31 (9): 1568-1573 [Abstract] ( 122 ) HTML (1 KB)  PDF (1160 KB)  ( 8 ) 1573 The Expression of Serum Nesfatin-1 and Omentin-1 in Patients with Polycystic Ovary Syndrome and Their Relationship with Insulin Resistance WEI Xinyi, et al DOI: 10.3969/j.issn.1006-6233.2025.09.029 Objective: To investigate the expression of serum Nesfatin-1 and Omentin-1 in patients with polycystic ovary syndrome (PCOS) and their relationship with insulin resistance (IR). Methods: From January 2023 to June 2024, 93 PCOS patients who received treatment in our hospital were included as the study group. According to the steady-state model of insulin resistance index (HOMA-IR), they were separated into a combined IR group of 42 cases and a non IR group of 51 cases. 58 healthy individuals who underwent physical examinations were included as the control group, and general data were collected. Fully automatic biochemical analyzer was applied to analyze serum biochemical indicators. ELISA method was applied to detect serum Nesfatin-1 and Omentin-1. Pearson correlation was applied to analyze the serum levels of Nesfatin-1 and Omentin-1 in the study group, and their correlation with IR related indicators. Logistic regression was applied to analyze the influencing factors of insulin resistance in PCOS patients. ROC curve was applied to analyze the diagnostic value of serum fasting insulin (FINS), Nesfatin-1, and Omentin-1 levels for insulin resistance in PCOS patients. Results: Compared to the control group, the research group exhibited significantly higher BMI (27.55±2.46 VS 23.28±2.07), waist-to-hip ratio (0.86±0.13 VS 0.78±0.11), FBG (4.79±1.23 VS 3.64±0.72), FINS (13.49±2.56 VS 9.62±2.05), HOMA-IR (2.93±0.74 VS 1.56±0.31), TC (4.36±0.93 VS 3.95±0.85), TG (2.26±0.55 VS 1.46±0.26), and LDL-C (2.95±0.45 VS 2.48±0.39). Meanwhile, the expression levels of HDL-C (1.14±0.25 VS 1.28±0.29), Nesfatin-1 (18.27±4.39 VS 29.83±6.88), and Omentin-1 (64.59±8.39 VS 82.16±14.88) were notably lower in the research group than in the control group (t=2.722~13.373, P＜0.05). The BMI (26.23±2.04 VS 28.64±2.37), waist-to-hip ratio (0.82±0.09 VS 0.89±0.11), FBG (3.88±0.86 VS 5.54±1.14), FINS (11.76±1.55 VS 14.91±2.24), HOMA-IR (2.03±0.64 VS 3.67±0.92), TC (4.15±0.69 VS 4.53±0.77), TG (2.06±0.38 VS 2.42±0.50), and LDL-C (2.83±0.31 VS 3.05±0.37) in the non-IR group were significantly lower than those in the combined IR group. Meanwhile, HDL-C (1.27±0.22 VS 1.03±0.18), Nesfatin-1 (20.65±3.16 VS 16.31±2.87), and Omentin-1 (71.94±7.72 VS 58.54±6.97) were significantly higher in the non-IR group than in the combined IR group (t=2.481~9.765, P＜0.05). Pearson analysis revealed that serum levels of Nesfatin-1 and Omentin-1 were negatively correlated with BMI, waist-to-hip ratio, FBG, FINS, TG, and LDL-C (r=-0.429, -0.495, -0.447, -0.378, -0.439, P<0.05) (r=-0.408, -0.508, -0.435, -0.632, -0.412, -0.419, P<0.05), positively correlated with HDL-C (r=0.445, P<0.05) (r=0.429, P<0.05), and positively correlated between Nesfatin-1 and Omentin-1 levels (r=0.485, P<0.05). Logistic analysis indicated that FINS [OR/95% CI=2.631 (1.051~6.584)], Nesfatin-1 [OR/95% CI=0.589 (0.430~0.808)], and Omentin-1 [OR/95% CI=0.697 (0.541~0.898)] were influencing factors of insulin resistance (IR) in patients with polycystic ovary syndrome (PCOS)(P<0.05). ROC analysis showed that the AUC of FINS, Nesfatin-1, and Omentin-1 levels for diagnosing IR in PCOS patients was 0.866, 0.871, and 0.869, respectively. The AUC of the combined diagnosis of the three was 0.953, which was obviously higher than that of the individual diagnosis (Z triple combination - FINS=2.069, P=0.039; Z triple combination - Nesfatin-1=2.037, P=0.042; Z triple combination - Omentin-1=1.998, P=0.046). Conclusion: Patients with Polycystic Ovary Syndrome (PCOS) exhibit downregulation of Nesfatin-1 and Omentin-1 levels, and their changes are closely correlated with insulin resistance (IR). The combined detection of these two factors and fasting insulin (FINS) levels holds high diagnostic value for PCOS combined with IR. 2025 Vol. 31 (9): 1573-1579 [Abstract] ( 120 ) HTML (1 KB)  PDF (1306 KB)  ( 6 ) 1579 Influencing Factors of Malnutrition-Inflammation-Atherosclerosis Syndrome in Patients with Maintenance Hemodialysis and Relationship with Prognosis LV Wenlu, LIU Ting, ZHU Xiaojing, et al DOI: 10.3969/j.issn.1006-6233.2025.09.030 Objective: To explore the occurrence and related influencing factors of malnutrition-inflammation-atherosclerosis syndrome (MIAS) in patients with maintenance hemodialysis (MHD), and to analyze the relationship between MIAS and prognosis in MHD patients, so as to provide evidence for the prevention and treatment of MIAS in MHD patients. Methods: The medical records of 160 MHD patients who received treatment in the hospital from March 2022 to March 2023 were retrospectively collected, and the occurrence of MIAS was investigated. Patients were divided into MIAS group (n=41) and non-MIAS group (n=119) according to whether MIAS occurred or not. The clinical data were compared between the two groups, The relationship of hypersensitive C-reactive protein (hs-CRP), carotid intima-media thickness (IMT) and albumin (ALB) with interleukin-6 (IL-6), transferrin (TRF), immunoinflammatory index (SII) and malnutrition-inflammation score (MIS) in MIAS patients was analyzed by pearson correlation coefficient. Logistic regression model was used to analyze the independent risk factors of MIAS in MHD patients. According to the 1-year follow-up results, MHD patients were divided into good prognosis group (n=132) and poor prognosis group (n=28), and the differences in clinical data and MIAS prevalence were compared between groups, and the relationship between MIAS and prognosis in MHD patient was explored by Logistic regression analysis. Results: MIAS occurred in 41 cases among 160 MHD patients, and the incidence of MIAS was 25.63%. Serum hs-CRP, IMT, IL-6, SII and MIS score in MIAS group were higher than those in non-MIAS group while serum ALB and TRF were lower than those in non-MIAS group (P<0.05). Serum hs-CRP and IMT in MHD patients were positively correlated with serum IL-6, SII and MIS score, and were negatively correlated with serum TRF (P<0.05). Serum ALB in MHD patients was negatively correlated with IL-6, SII and MIS score, and positively correlated with serum TRF (P<0.05). High serum IL-6, SII and MIS score, and low serum TRF were independent risk factors for MIAS in MHD patients (P<0.05). Serum IL-6, serum SII, MIS score and MIAS prevalence rate in poor prognosis group were higher than those in good prognosis group while serum TRF was lower than that in good prognosis group (P<0.05). Increased serum IL-6 and SII, decreased serum TRF, high MIS score and concurrent MIAS were independent risk factors of poor prognosis in MHD patients (P<0.05). Conclusion: MIAS is common in MHD patients. Increased serum IL-6, SII and MIS score and decreased serum TRF are independent risk factors of MIAS and poor prognosis in MHD patients, and MIAS is closely related to prognosis in MHD patients. 2025 Vol. 31 (9): 1579-1584 [Abstract] ( 136 ) HTML (1 KB)  PDF (1117 KB)  ( 19 )

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