Abstract：Objective: To discuss the impacts of micro non-coding RNA-516b-5p (miR-516b-5p) on the proliferation and invasion of breast cancer (BC) cells by targeting NOP2/Sun RNA methyltransferase 2 (NSUN2). Methods: qRT-PCR experiments were performed to detect the miR-516b-5p and NSUN2 mRNA in BC cancer tissues, adjacent tissues, normal breast epithelial cells (MCF-10A), and BC cells (MCF-7, MDA-MB-231, HCC1937). MCF-7 cells were assigned into NC group, miR-NC group, miR-516b-5p mimic group, sh-NC group, sh-NSUN2 group, miR-516b-5p mimic+pcDNA-NC group, and miR-516b-5p mimic+pcDNA-NSUN2 group. Clone formation and Transwell invasion experiments were performed to detect the proliferation and invasion abilities of MCF-7 cells in each group. Western blot was performed to detect the NSUN2, cyclin 1 (CCND1), cyclin dependent kinase 1 (CDK1), matrix metalloproteinase 2 (MMP2), and matrix metalloproteinase 9 (MMP9) proteins. The nude mouse BC transplant tumor model was constructed and separated into Model group, miR-516b-5p mimic group, and miR-516b-5p mimic+pcDNA-NSUN2 group. The tumor mass and volume were measured in each group. Results: For adjacent tissues, the miR-516b-5p in cancer tissues was unusually declined, while the NSUN2 mRNA was clearly increased (P<0.05). Compared with MCF-10A cells, the miR-516b-5p in MCF-7, MDA-MB-231, and HCC1937 cells declined clearly, while the NSUN2 mRNA raised unusually (P<0.05), with the most unusual trend observed in MCF-7 cells. For the NC group, there were no unusual changes in various cellular indicators in the miR-NC group and sh-NC group (P>0.05). For the miR-NC group, the miR-516b-5p mimic group showed declined the number of colony forming cells and invasive cells, unusually decreased NSUN2 mRNA, and NSUN2, CCND1, CDK1, MMP2, and MMP9 proteins, and clearly raised miR-516b-5p (P<0.05). For the sh-NC group, the changes in various indicators in the sh-NSUN2 group were consistent with those in the miR-516b-5p mimic group (P<0.05). The trend of changes in various indicators in the miR-516b-5p mimic+pcDNA-NSUN2 group was opposite to that in the miR-516b-5p mimic group (P<0.05). The dual luciferase assay confirmed a targeted relationship between miR-516b-5p and NSUN2 (P<0.05). In the nude mouse tumorigenesis experiment, the miR-516b-5p mimics group showed a clear reduction in tumor tissue mass and volume than the Model group (P<0.05). For the miR-516b-5p mimic group, the miR-516b-5p mimic+pcDNA-NSUN2 group showed a prominent increase in tumor tissue mass and volume (P<0.05). Conclusion: MiR-516b-5p can inhibit BC cell proliferation and invasion by targeting and downregulating NSUN2.
程欣然, 范亚楠, 刘俊, 皮亚平. miR-516b-5p靶向NSUN2对乳腺癌细胞增殖和侵袭的影响[J]. 河北医学, 2025, 31(10): 1585-1592.
CHENG Xinran, FAN Ya'nan, LIU Jun, et al. Impacts of miR-516b-5p on Proliferation and Invasion of Breast Cancer Cells by Targeting NSUN2. HeBei Med, 2025, 31(10): 1585-1592.
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2025, Vol. 31 
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