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2025 Vol. 31, No. 6 Published: 2025-06-30 881 Effect of RNF8 Regulation of Wnt/β-catenin Signalling Pathway on Proliferation Invasion Migration and Epithelial Mesenchymal Transition of Renal Clear Cell Carcinoma Cells ZHANG Yali, ZHANG Xiaoyan, ZHAO Junlin DOI: 10.3969/j.issn.1006-6233.2025.06.01 Objective: To explore the effects of RNF8 on the proliferation, invasion, migration, EMT of CCRCC cells, as well as its regulatory mechanism on the Wnt/β-catenin signaling pathway. Methods: Thirty specimens of cancer and paracancerous tissues from CCRCC patients who attended and underwent pathological examination at Taiyuan Central Hospital from 2018 to 2023 were collected; RNF8 mRNA expression levels in paracancerous tissues, cancerous tissues, and human CCRCC cancer cells 786-O were detected by qRT-PCR; human CCRCC cell line 786-O cells were randomly divided into the NC group (untransfected with plasmidThe human CCRCC cell line 786-O cells were randomly divided into NC group (not transfected with plasmid), si-NC group (transfected with RNF8 negative control), si-RNF8 group (transfected with si-RNF8), and si-RNF8+Wnt activator LiCl (si-RNF8+LiCl) group; the proliferation of cells was detected by using the CCK-8 assay and the clone formation assay; the number of migrating and invasive cells in the groups was detected by using the Transwell assay; and the number of cells that had migrated and invaded in the groups was detected by using theWestern blot assay was used to detect the protein expression levels of Wnt/β-catenin signalling pathway and EMT in the cells. Results: The expression level of RNF8 mRNA in CCRCC cancer tissue (1.47±0.12) was higher than that in adjacent tissues (0.62±0.05) (t=35.813, P<0.001). Compared with the si-NC group, the expression levels of RNF8 mRNA, Wnt1, β-catenin, Vimentin, and N-cadherin, cell survival rate, clone formation number, and the numbers of cells undergoing migration and invasion in the si-RNF8 group were greatly lower (P<0.05), while the expression level of E-cadherin higher (P<0.05). The addition of Wnt activator LiCl reversed the inhibitory effect of silencing RNF8 expression on proliferation, invasion, migration, and EMT of 786-O cells (P<0.05). Conclusion: RNF8 is highly expressed in CCRCC cancer tissues, and knocking down RNF8 can inhibit the proliferation, invasion, migration, and EMT of CCRCC cancer cells, which may be related to the inhibition of the Wnt/β - catenin signaling pathway. 2025 Vol. 31 (6): 881-885 [Abstract] ( 14 ) HTML (1 KB)  PDF (1981 KB)  ( 8 ) 886 Relationship between CYP2C19 Gene Polymorphism Serum NSE TNF-α and Clinical Outcome of Clopidogrel Treatment in Cerebral Infarction ZHUANG Yuan, FAN Weiwei, LIU Dunhua DOI: 10.3969/j.issn.1006-6233.2025.06.02 Objective: To observe the association of serum neuron-specific enolase (NSE), tumor necrosis factor-α (TNF-α) and CYP2C19 gene polymorphism with clopidogrel treatment outcome in patients with cerebral infarction. Methods: A total of 104 patients with cerebral infarction in the hospital were enrolled from November 2021 to November 2023, and were treated with clopidogrel. CYP2C19 gene polymorphism and levels of NSE and TNF-α were detected, and the relationship with clopidogrel treatment outcome was analyzed. Results: There was a statistical difference in the distribution of CYP2C19 gene metabolic type between good prognosis group and poor prognosis group (P<0.05). The platelet aggregation rate and serum NSE and TNF-α levels in poor prognosis group were higher than those in good prognosis group, with statistical differences (P<0.05). The platelet aggregation rate, NSE and TNF-α levels revealed statistical differences among different metabolic types of CYP2C19 gene (P<0.05). The platelet aggregation rate, NSE and TNF-α of slow metabolic type and intermediate metabolic type were higher than those of fast metabolic type, with statistical differences (P<0.05). The proportion of patients with diabetes mellitus and age were higher or older in poor prognosis group than those in good prognosis group, with statistical differences (P<0.05). Multivariate Logistic regression analysis suggested that diabetes mellitus, CYP2C19 gene slow metabolic type, age, NSE and TNF-α were risk factors affecting the clopidogrel treatment outcome (P<0.05). ROC curve analysis revealed that the AUC, sensitivity and specificity of the combined evaluation of the five indicators were 0.843, 0.953 and 0.574 respectively. Conclusion: The poor prognosis of patients with cerebral infarction after clopidogrel treatment is related to the slow metabolic type of CYP2C19 gene and the levels of serum NSE and TNF-α, and has certain evaluated value on evaluating the treatment outcome. 2025 Vol. 31 (6): 886-891 [Abstract] ( 10 ) HTML (1 KB)  PDF (1265 KB)  ( 6 ) 891 Relationship between Expression Levels of β-catenin c-Myc and Wnt1 mRNA in Tissues and Pathological Grading and Postoperative Recurrence in Patients with CIN Combined with HPV Infection LIANG Xiao, HAN Dongfang, REN Jing, et al DOI: 10.3969/j.issn.1006-6233.2025.06.03 Objective: To explore and analyze the expressions of β-catenin, C-Myelocytomatosis viral oncogene homolog (c-Myc) and wingless MMTV integration site family member 1 (Wnt1) in cervical tissues of patients with cervical intraepithelial neoplasia (CIN) combined with human papillomavirus (HPV) infection, and to analyze its relationship with pathological grading and postoperative recurrence. Methods: Totally 92 patients with CIN combined with HPV infection in our hospital from January 2020 to December 2023 were included. The mRNA expression levels of β-catenin, c-Myc and Wnt1 in cervical tissues were determined by real-time fluorescence quantitative PCR, and the association between their expressions and pathological grading and postoperative recurrence was analyzed. Results: The comparison of the expression levels of β-catenin, c-Myc and Wnt1 mRNA in tissues of patients with different CIN grades showed statistically significant differences (P<0.05), and those in CIN grade 3 and CIN grade 2 were higher than those in CIN grade 1, while those in CIN grade 3 were higher than those in CIN grade 2, and the differences were all statistically significant (P<0.05). Spearman correlation showed that the expression levels of β-catenin, c-Myc, and Wnt1 mRNA in tissues were positively correlated with the CIN grade of cervical tissue biopsy (r=0.527, 0.568, 0.574, P<0.05). Ordered Logistic regression showed that the expression levels of tissue β-catenin, c-Myc, and Wnt1 mRNA were risk factors affecting the pathological grade of CIN in cervical tissue biopsy (P<0.05). The expression levels of β-catenin, c-Myc and Wnt1 mRNA in the tissues of patients with postoperative recurrence were higher than those of non-recurrence patients, and the difference was statistically significant (P<0.05). ROC curves revealed that the AUCs of tissue β-catenin, c-Myc and Wnt1 mRNA expression levels in predicting postoperative recurrence were 0.799, 0.849 and 0.795, the sensitivities were 0.941, 0.941 and 0.706, and the specificities were 0.589, 0.649 and 0.821, respectively. Conclusion: The mRNA expression levels of β-catenin, c-Myc and Wnt1 in cervical tissues of CIN patients are related to the pathological grading and postoperative recurrence, and have certain predictive value on postoperative recurrence. 2025 Vol. 31 (6): 891-896 [Abstract] ( 13 ) HTML (1 KB)  PDF (1351 KB)  ( 2 ) 896 Relationship between Serum GDF-15 and FGF23 Expression with Nutritional Status and Prognosis in Elderly Esophageal Cancer Patients XUE Qian, TIAN Ruiyuan DOI: 10.3969/j.issn.1006-6233.2025.06.04 Objective: To explore the relationship between the expression of serum growth differentiation factor-15 (GDF-15) and fibroblast growth factor 23 (FGF23) in elderly patients with esophageal cancer and their nutritional status and prognosis. Methods: A total of 117 elderly patients with esophageal cancer (case group) and 117 elderly healthy individuals (control group) admitted to our hospital from February 2019 to February 2021 were selected as the research subjects. The expression levels of GDF-15 and FGF23 in the serum of elderly patients with esophageal cancer were detected by ELISA, and the relationship between the two and clinical pathological characteristics was analyzed; the correlation between the levels of GDF-15 and FGF23 and nutritional status indicators [Nutritional Risk Screening 2002 (NRS-2002) score, Patient-Generated Subjective Global Assessment (PG-SGA)] were analyzed by Spearman method; Logistic regression was used to analyze the influencing factors of death in elderly patients with esophageal cancer; the value of GDF-15 and FGF23 in predicting the prognosis of elderly patients with esophageal cancer was analyzed by ROC curve. Results: Compared with the control group, the levels of GDF-15 and FGF23 and the NRS-2002 and PG-SGA scores in the case group were increased (P<0.05). The expression levels of GDF-15 and FGF23 were related to lymph node metastasis and TNM stage (P<0.05). The results of Spearman correlation analysis showed that GDF-15 was positively correlated with NRS-2002 and PG-SGA scores (P<0.05), and FGF23 was positively correlated with NRS-2002 and PG-SGA scores (P<0.05). The expression levels of GDF-15 and FGF23 and the NRS-2002 and PG-SGA scores in the death group were higher than those in the survival group (P<0.05). Multivariate Logistic regression analysis showed that lymph node metastasis, GDF-15, and FGF23 were risk factors affecting the prognosis of death in elderly patients with esophageal cancer (P<0.05). The ROC curve analysis showed that the AUCs of GDF-15 and FGF23 levels in predicting the prognosis of death in patients were 0.878 and 0.767, respectively, and the AUC of the combined prediction of the two was 0.920, which was greater than the AUCs of the two predictions alone (P<0.05). Conclusion: The expression levels of GDF-15 and FGF23 in the serum of elderly patients with esophageal cancer are elevated, and their expression levels are related to lymph node metastasis, TNM stage, and nutritional status. The combination of the two can be used as an effective serological detection tool to predict the prognosis of patients. 2025 Vol. 31 (6): 896-901 [Abstract] ( 11 ) HTML (1 KB)  PDF (1340 KB)  ( 2 ) 902 Factors Influencing the Occurrence of Adverse Pregnancy Outcomes and Their Predictive Value in Patients after Laparoscopic Surgery for Endometriosis SHI Shujing, ZHANG Chunyan, PEI Lihua, et al DOI: 10.3969/j.issn.1006-6233.2025.06.05 Objective: To investigate the factors influencing adverse pregnancy outcomes after laparoscopic surgery in patients with endometriosis and their clinical predictive value. Methods: Clinical data were collected and retrospectively from 124 patients with endometriosis (EMS) who underwent laparoscopic surgery at our hospital from December 2019 to December 2022. The patients were divided into two groups based on their pregnancy outcomes within 2 years after surgery: the good outcome group (77 cases) and the adverse outcome group (47 cases). Univariate analysis was used to compare the age, infertility duration, revised American Fertility Society (r-AFS) staging, Endometriosis Fertility Index (EFI), postoperative medication, postoperative anti-Mullerian hormone (AMH), serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and progesterone (P) levels between the two groups. Multivariate Logistic regression was used to analyze the independent influencing factors of adverse pregnancy outcomes after laparoscopic endometriosis. ROC curve analysis was used to evaluate the predictive value of related indicators, and a nomogram prediction model was constructed and assessed. Results: The age and E2 in the poor outcome group were both greater than those in the good outcome group (P<0.05). The levels of EFI, AMH and P in the poor outcome group were all lower than those in the good outcome group (P<0.05). The results of multivariate analysis showed that EFI score, AMH, E2 and P levels were all independent influencing factors for adverse pregnancy outcomes in patients after laparoscopic surgery (P<0.05). The combination of the four (AUC=0.909) had the highest predictive value for adverse pregnancy outcomes in patients after laparoscopic surgery. The nomogram prediction model constructed based on independent influencing factors has good prediction accuracy and high clinical application value. Conclusion: Low EFI scores, low AMH and P levels, and high E2 levels after surgery can be considered as auxiliary indicators for adverse pregnancy outcomes after laparoscopic surgery in patients with endometriosis, and they are independent influencing factors. The combination of these four factors has the highest predictive value. 2025 Vol. 31 (6): 902-907 [Abstract] ( 7 ) HTML (1 KB)  PDF (1586 KB)  ( 3 ) 908 Relationship of Peripheral Blood IgE 25-(OH)-D and Electroencephalogram Changes with Prognosis in Children with Tic Disorders ZHANG Yan, XIAO Guohui, CHEN Jinxu, et al DOI: 10.3969/j.issn.1006-6233.2025.06.06 Objective: To analyze peripheral blood immunoglobulin E (IgE) and 25-hydroxyvitamin D [25-(OH)-D], electroencephalogram (EEG) changes in relation to prognosis in children with tic disorders (TD). Methods: Totally 102 children with TD admitted to the hospital from May 2021 to March 2024 were retrospectively selected. According to the Yale Global Tic Severity Scale (YGTSS) score, children enrolled were divided into the mild to moderate group (YGTSS score ≤ 50) and the severe group (YGTSS score >50). The levels of IgE and 25-(OH)-D, and EEG examination results in the two groups were compared. After standardized treatment, the children were divided into good prognosis group and poor prognosis group based on the decrease in YGTSS score. Multivariate logistic regression analysis was performed to screen the prognostic factors for children with TD, and discuss their correlation with EEG examination results, IgE, and 25-(OH)-D. Results: Among 102 children with TD, 78 (76.47%) and 24 (23.53%) had mild to moderate and severe disease respectively. There were statistically significant differences in the rates of IgE, 25-(OH)-D and EEG abnormalities between the two groups. The abnormal rates of IgE and EEG in the severe group were significantly higher than those in the mild to moderate group, and 25-(OH)-D level was lower than the mild to moderate group (P<0.05). Among 102 children with TD, 41 had poor prognosis, accounting for 40.20%. IgE level, and abnormal rate of EEG in the poor prognosis group were significantly higher than those in the good prognosis group, and 25-(OH)-D level was significantly lower than that in the good prognosis group (P<0.05). Logistic analysis found that EEG examination results, IgE, and 25-(OH)-D were independent risk factors influencing the prognosis of TD (P<0.05). Conclusion: Peripheral blood IgE levels in children with TD are relatively high. Besides, the lower 25-(OH)-D level, the higher the abnormal rate of EEG. All of above indicators are closely related to the severity and prognosis of the disease. Detecting these indicators is of great significance for evaluating disease progression and therapeutic effects. 2025 Vol. 31 (6): 908-912 [Abstract] ( 6 ) HTML (1 KB)  PDF (1203 KB)  ( 1 ) 912 Relationship between Homocysteine Platelet-Related Parameters and Clinical Outcomes in Patients with Acute Cerebral Infarction after Emergency Interventional Thrombolysis ZHANG Pan, BAI Jie, XU Yinmei, et al DOI: 10.3969/j.issn.1006-6233.2025.06.07 Objective: To observe the relationship between changes of serum homocysteine (Hcy) and platelet-related parameters and clinical outcomes after emergency interventional thrombolysis in patients with acute cerebral infarction (ACI). Methods: Totally 116 patients with ACI in the hospital were included from August 2022 to June 2024. All enrolled patients received emergency interventional thrombolytic therapy. The changes of serum Hcy and platelet-related parameters [platelet count (PLT), membrane glycoprotein p-selectin (P-selection, CD62P), platelet distribution width (PDW)] were observed before intervention, and the relationship with clinical outcomes of patients was analyzed. Results: The levels of Hcy, CD62P and PDW in patients with poor prognosis were higher than those in patients with good prognosis (P<0.05) while the PLT level was lower than that in patients with good prognosis (P<0.05). Spearman correlation analysis indicated that Hcy, CD62P and PDW were positively correlated with modified Rankin scale (mRS) score (r=4.766, 3.862, 4.268, P<0.05), and PLT level was negatively correlated with mRS score (r=-3.984, P<0.05). Univariate analysis revealed that diabetes mellitus, age, time from onset to admission, and National Institutes of Health Stroke Scale (NIHSS) score were associated with clinical outcomes of ACI patients (P<0.05). Logistic regression analysis found that diabetes mellitus, age, time from onset to admission, NIHSS score, Hcy, CD62P and PDW were risk factors affecting the clinical outcomes of patients (P<0.05), and PLT level was a protective factor (P<0.05). ROC curve demonstrated that the AUCs of Hcy, PLT, CD62P and PDW in evaluating the clinical outcomes were 0.816, 0.744, 0.732 and 0.804 respectively. Conclusion: The abnormal levels of Hcy, PLT, CD62P and PDW are related to clinical outcomes of ACI patients with emergency interventional thrombolysis, and have certain evaluated value. 2025 Vol. 31 (6): 912-917 [Abstract] ( 6 ) HTML (1 KB)  PDF (1350 KB)  ( 1 ) 917 Risk Factors for Recurrence and Their Predictive Value after Laparoscopic Myomectomy in Patients with Uterine Fibroids DONG Xiaoli, OU Chaoyang, GENG Chen, et al DOI: 10.3969/j.issn.1006-6233.2025.06.08 Objective: To analyze the risk factors for recurrence in patients with uterine fibroids after laparoscopic myomectomy, and to explore the predictive value of risk factors on postoperative recurrence. Methods: Totally 103 patients with uterine fibroids who received laparoscopic myomectomy in the hospital were selected from February 2019 to June 2022 as the research subjects. According to the recurrence status within 2 years after surgery, the patients were classified into recurrence group (n=25) and non-recurrence group (n=78). The clinical data of all study subjects were collected, and the risk factors affecting recurrence after laparoscopic myomectomy were explored by univariate analysis and Logistic multivariate regression analysis. ROC curve was plotted to analyze the predictive value of risk factors on postoperative recurrence. Results: Univariate analysis suggested that there were statistical differences in age, menarche age, number of uterine fibroids, diameter of uterine fibroids and postoperative estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) between the recurrence group and the non-recurrence group (P<0.05). Logistic regression analysis indicated that age≥35 years old, menarche age<14 years old, number of uterine fibroids≥2, maximum fibroid diameter≥10 cm and postoperative FSH and LH were high risk factors for postoperative recurrence (P<0.05), and postoperative E2 was a protective factor (P<0.05). ROC curve showed that the area under the curve (AUC) of the combined prediction of postoperative recurrence by age, age of menarche, number of uterine fibroids and maximum fibroid diameter was 0.996, sensitivity was 100.00% and specificity was 94.87%, which was significantly better than that predicted by each factor alone (P<0.05). Conclusion: The recurrence after laparoscopic myomectomy is affected by factors such as patient age, menarche age, number of uterine fibroids, maximum fibroid diameter and hormones levels. The prediction model based on the above risk factors has good predictive value on postoperative recurrence. 2025 Vol. 31 (6): 917-923 [Abstract] ( 6 ) HTML (1 KB)  PDF (1401 KB)  ( 1 ) 923 Effect of Different Surgical Modalities for T1b Stage Renal Cancer on Patients' Postoperative Recurrence Metastasis and CTC REN Lei, SHI Liang, YAN Qunfeng, et al DOI: 10.3969/j.issn.1006-6233.2025.06.09 Objective: To investigate the influence of different surgical treatments on postoperative recurrence and metastasis and circulating tumor cell (CTC) in patients with stage T1b renal cell carcinoma (RCC). Methods: The case data of 88 patients with stage T1b RCC were retrospectively analyzed. According to different treatment methods, 42 patients undergoing partial nephrectomy were included in group A, and 46 patients receiving radical nephrectomy were enrolled as group B. The surgery-related indicators, renal function recovery conditions [estimated glomerular filtration rate (eGFR)], CTC level, postoperative recurrence and metastasis status [recurrence-free survival (RFS) rate within 3 years] and occurrence of postoperative complications were observed in the two groups. Results: There was no statistical difference in the intraoperative hemorrhagic fever time between groups (P>0.05). Compared with group B, group A had longer surgical time, less intraoperative estimated blood loss, and shorter postoperative hospital stay (P<0.05). eGFR level in group A at 1, 3 and 6 months after surgery had no statistical difference compared with that before surgery (P>0.05), eGFR level in group B at 1 month after surgery had no statistical difference compared to before surgery (P>0.05), and eGFR level at 3 and 6 months after surgery was decreased (P<0.05). The eGFR level in group A was not statistically different from group B at 1 month after surgery (P>0.05), and the eGFR level in group A was higher than that in group B at 3 and 6 months after surgery (P<0.05). The CTC count in both groups was increased at 7 days after surgery (P<0.05), but no statistical difference was shown in the CTC count at 3 months after surgery (P>0.05). The differences between the two groups were not statistical in terms of CTC count at various time points after surgery, RFS within 3 years and total incidence rate of postoperative complications (P>0.05). Conclusion: Both partial nephrectomy and radical nephrectomy are safe and effective in the treatment of patients with stage T1b RCC, with few postoperative complications. Both methods have the same effect on controlling tumor recurrence and metastasis. However, compared with radical nephrectomy, partial nephrectomy can better protect the renal function of patients and promote postoperative recovery. 2025 Vol. 31 (6): 923-928 [Abstract] ( 8 ) HTML (1 KB)  PDF (1241 KB)  ( 2 ) 929 Mechanistic Study of T-Cadherin in Multidrug Resistance of Melanoma LU Haitao, LI Xuefei, NIU Yandong, et al DOI: 10.3969/j.issn.1006-6233.2025.06.10 Objective: To investigate the role and underlying mechanism of T-cadherin in multidrug resistance of melanoma. Methods: The sensitivity of the cisplatin-resistant B16F10 (CDDP-R B16F10) melanoma cell line to dacarbazine (DTIC) and paclitaxel (PTX) was assessed. The effects of T-cadherin in combination with DTIC and PTX on cell proliferation and migration were evaluated using the MTT assay and wound healing assay. qRT-PCR and Western blot analyses were performed to determine the impact of T-cadherin on the expression of multidrug resistance gene 1 (MDR1) and Survivin in CDDP-R B16F10 cells. Results: The resistance index (RI) of CDDP-R B16F10 cells was 3.46 for DTIC and 1.46 for PTX. Compared to other groups, the combination of T-cadherin with DTIC and PTX significantly reduced the OD value and wound healing rate (P<0.05). The relative mRNA and protein expression levels of MDR1 and Survivin were significantly higher in CDDP-R B16F10 cells than in parental B16F10 cells (P<0.05). However, T-cadherin transfection markedly decreased MDR1 and Survivin expression at both the mRNA and protein levels in CDDP-R B16F10 cells compared to the non-transfected groups (P<0.05). Conclusion: The multidrug resistance of CDDP-R B16F10 cells is induced by prolonged exposure to escalating doses of CDDP. T-cadherin enhances the sensitivity of CDDP-R B16F10 cells to DTIC and PTX and reverses multidrug resistance by downregulating MDR1 and Survivin expression. 2025 Vol. 31 (6): 929-934 [Abstract] ( 9 ) HTML (1 KB)  PDF (1669 KB)  ( 2 ) 934 The Effect of Qilong Healing Ointment on Wound Repair in Diabetic Foot Rat Model Based on SIRT1-Mediated Autophagy CAI Xinlun, ZHU Yan, LI Jun, et al DOI: 10.3969/j.issn.1006-6233.2025.06.11 Objective: To explore the effect and molecular mechanism of Qilong Healing Ointment on wound repair in diabetic foot rat model based on SIRT1 signaling molecule and autophagy. Methods: Male SD rats were randomly divided into control group, model group, positive drug metformin group, Qilong healing ointment group, and Qilong healing ointment + SIRT1 inhibitor EX527 group, with 8 rats in each group. A rat model of diabetic foot was constructed by feeding with high sugar and high fat, intraperitoneal injection of streptozotocin chain, and excision of dorsum skin of the foot. Continuous glucose meter to detect fasting blood glucose (FBG) in rats. The wound healing rate of rats was observed and calculated. HE staining was used to detect pathological changes in wound tissues. The levels of IL-6, TNF-α, IL-1β in serum were detected by ELISA. The expression of SIRT1, Beclin1, p62, LC3 mRNAs in wound tissue were detected by RT-qPCR. The expression of SIRT1, Beclin1, p62, LC3Ⅱ/Ⅰ proteins in wound tissue was detected by Western blot. An immunohistochemical staining assay was used to detect the expression of SIRT1 protein in wound tissues. Results: Compared with the control group, the level of FBG in the model group increased (P<0.05), the wound healing rate decreased (P<0.05), a large number of inflammatory cells were infiltrated in the wound tissue and the neovascularization and fibroblasts significantly reduced, the levels of IL-6, TNF-α, IL-1β in serum increased (P<0.05), the expression of SIRT1, Beclin1, LC3Ⅱ/Ⅰ decreased in wound tissue and p62 increased (P<0.05). Compared with the model group, the level of FBG in the positive drug group and the Qilong healing ointment group decreased (P<0.05), the wound healing rate increased (P<0.05), the infiltration of inflammatory cells in the wound tissue decreased and the number of neovasculars and fibroblasts increased, the levels of IL-6, TNF-α, IL-1β in serum decreased (P<0.05), the expression of SIRT1, Beclin1, LC3Ⅱ/Ⅰ increased in wound tissue and p62 decreased (P<0.05). EX527 significantly inhibits the effect of Qilong Healing Ointment (P<0.05). Conclusion: Qilong Healing Ointment can promote autophagy and inhibit inflammatory response to alleviate the damage of wound tissue in rats with diabetic foot, and its mechanism may be related to the activation of SIRT1 signaling. 2025 Vol. 31 (6): 934-940 [Abstract] ( 6 ) HTML (1 KB)  PDF (1813 KB)  ( 1 ) 941 Improvement Effect of Self-Made Danggui Zhuyu Decoction on Gastroesophageal Reflux Disease Rats Based on NF-κB/MAPK Signaling Pathway WANG Lisheng, ZHOU Ning, HUANG Minzhe, et al DOI: 10.3969/j.issn.1006-6233.2025.06.12 Objective: To explore the improvement effect of Danggui Zhuyu decoction on gastroesophageal reflux disease (GERD) rats and to preliminarily investigate its possible mechanism based on the NF-κB/MAPK signaling pathway. Methods: The GERD rat model was established by esophageal stent implantation. The rats were randomly divided into a sham operation group, a model group, a western medicine group (Moxapride citrate dispersed tablet 1.56 mg/kg and Lansoprazole enteric-coated tablet 3.125 mg/kg, gavage), Danggui Zhuyu decoction group (3.0 mg/kg Danggui Zhuyu decoction), with 15 rats in each group (3.0 mg/kg Angelica Chuyu Decoction + 10 mg/kg intraperitoneal injection of NF-κB pathway activator BA). The general state of each group was recorded during treatment. Hematoxylin-eosin (H&E) staining was used to analyze pathological histology of esophageal tissues. ELISA kit was applied to detect levels of TNF-α, IL-6, and IL-1β in esophageal tissues.RT-qPCR was performed to detect mRNA levels of macrophage polarization markers CD86, iNOS, and CD206 in esophageal tissues. Western Blot was used to detect expressions of E-cadherin, Claudin-4, and proteins related to NF-κB/MAPK signaling pathway. Results: Compared with the sham operation group, the model group had dull hair, reduced food intake, water intake, and body weight, dry feces with decreased volume, food residue reflux to the corners of the mouth, squamous epithelial hyperplasia in esophageal mucosa, lamina propria papilla extension, massive diffuse inflammatory cell infiltration, significantly increased TNF-α, IL-6, IL-1β, CD86 and iNOS mRNA, p-p65, p-JNK, p-ERK (P<0.05), and significantly decreased CD206 mRNA, E-cadherin, and Claudin-1 (P<0.05). Compared with the model group, the western medicine group, Danggui Zhuyu decoction group, and NF-κB activator group showed improved hair gloss, food intake, water intake, and feces condition, reduced dry or moist feces, decreased food residue reflux, increased body weight, attenuated esophageal mucosal squamous epithelial hyperplasia, lamina propria elongation, and inflammatory cell infiltration, significantly decreased TNF-α, IL-6, IL-1β, CD86 and iNOS mRNA, p-p65, p-JNK, p-ERK (P<0.05), and significantly increased CD206 mRNA, E-cadherin, and Claudin-1 (P<0.05). Compared with the western medicine group, the Danggui Zhuyu decoction group and NF-κB activator group had slight mucosal squamous epithelial hyperplasia and inflammatory cell infiltration, reduced lamina propria extension, significantly decreased TNF-α, IL-6, IL-1β, CD86 and iNOS mRNA, p-p65, p-JNK, p-ERK (P<0.05), and significantly increased CD206 mRNA, E-cadherin, and Claudin-1 (P<0.05). Compared with the Danggui Zhuyu decoction group, the NF-κB activator group had more inflammatory cell infiltration, squamous epithelial hyperplasia, and lamina propria extension, significantly increased TNF-α, IL-6, IL-1β, CD86 and iNOS mRNA, p-p65, p-JNK, p-ERK (P<0.05), and significantly decreased CD206 mRNA, E-cadherin, and Claudin-1 (P<0.05). Conclusion: Danggui Zhiru Decoction can improve GERD, regulate macrophage polarization to inhibit inflammatory responses, and enhance the function of the epithelial mucosal barrier. Its mechanism may be related to the inhibition of the NF-κB/MAPK signaling pathway. 2025 Vol. 31 (6): 941-947 [Abstract] ( 7 ) HTML (1 KB)  PDF (1637 KB)  ( 1 ) 947 Correlation and Predictive Value Analysis of 18F-FDG PET/CT Quantitative Parameters with the Prognosis and Outcomes of DLBCL LI Yubin, ZHANG Qizhou, LI Xiaohong DOI: 10.3969/j.issn.1006-6233.2025.06.13 Objective: To analyze the correlation and predictive value of quantitative parameters of 18F-fluorodeoxyglucose positron-emission tomography compared with computed tomography (18F-FDG PET/CT) with the prognosis and outcome of diffuse large B-cell lymphoma (DLBCL). Methods: A total of 200 DLBCL patients from The First Affiliated Hospital of Xinjiang Medical University from May 2020 to May 2022 were selected. All patients underwent 18F-FDG PET/CT examination to obtain quantitative parameters. After a 2-year follow-up, patients were divided into a poor prognosis group and a good prognosis group according to their prognosis. The general information and 18F-FDG PET/CT quantitative parameters [maximum standard uptake value (SUVmax), average standard uptake value (SUVmean), maximum metabolic tumor volume (MTVmax), total metabolic tumor volume (MTVsum), maximum glycolysis (TLGmax), total glycolysis (TLGsum), maximum diameter of the largest lesion (Dmax)] were compared between the two groups. Logistic regression analysis was used to analyze the factors affecting poor prognosis. A conventional logistic prediction model was established based on conventional influencing factors. A new logistic prediction model was established by combining conventional influencing factors with quantitative parameters of 18F-FDG PET/CT. The predictive value of the two logistic prediction models was analyzed using the receiver operating characteristic (ROC) curve. Results: Among the 192 patients who completed follow-up, 81 patients had a poor prognosis (42 relapsed, 23 progressed, and 16 died), and 111 patients had a good prognosis. The proportion of Ann-Arbor stage Ⅲ~Ⅳ, the proportion of International Prognostic Index (IPI) score ≥4, the lactate dehydrogenase/albumin ratio (LAR), and the MTVmax, MTVsum, TLGmax, TLGsum, Dmax in the poor prognosis group were higher than those in the good prognosis group, while the albumin/fibrinogen ratio (AFR) was lower than that in the good prognosis group, with statistically significant differences (P<0.05). Logistic regression analysis showed that Ann-Arbor staging (OR=2.129, 95%CI: 1.426~3.180), IPI (OR=2.640, 95%CI: 1.731~4.025), AFR (OR=0.383, 95%CI: 0.267~0.549), LAR (OR=1.420, 95%CI: 1.143~1.765), MTVmax (OR=1.503, 95%CI: 1.206~1.873), MTVsum (OR=1.445, 95%CI: 1.185~1.761), TLGmax (OR=1.487, 95%CI: 1.193~1.854), TLGsum (OR=1.455, 95%CI: 1.175~1.802), Dmax (OR=1.519, 95%CI: 1.220~1.891) were all factors affecting the poor prognosis of DLBCL patients (P<0.05). A logistic prediction model was constructed, and ROC analysis showed that the area under the curve (AUC) of the new model for predicting poor prognosis in DLBCL patients was 0.941 (95%CI: 0.897~0.970), which was superior to the predictive performance of conventional models (P<0.05). Conclusion: The 18F-FDG PET/CT quantitative parameters of MTVmax, MTVsum, TLGmax, TLGsum, and Dmax are all factors influencing the poor prognosis of patients with DLBCL, and the combination of them with conventional indices can significantly increase the predictive value of the risk of poor prognosis, which can be used as a new protocol for clinical prediction of prognosis and guide clinical decision-making. 2025 Vol. 31 (6): 947-953 [Abstract] ( 7 ) HTML (1 KB)  PDF (1435 KB)  ( 1 ) 954 Amplitude-Integrated Electroencephalographic Changes in Preterm Infants of Different Gestational Ages and Their Relation to Brain Development WANG Xuehong, WANG Juan, CHEN Dan, et al DOI: 10.3969/j.issn.1006-6233.2025.06.14 Objective: To explore the relationship between changes of amplitude-integrated electroencephalogram (aEEG) and brain development in premature infants at different gestational ages. Methods: A total of 103 premature infants admitted to the hospital from January 2023 to April 2024 were selected as the research subjects. They were grouped according to gestational age. The changes of aEEG were analyzed. All subjects underwent multimodal magnetic resonance imaging (MRI) examination, and diffusion tensor imaging (DTI) was used to quantitatively evaluate their brain development. Spearman method was used to analyze the relationship between aEEG changes and brain development in premature infants at different gestational ages. Results: As gestational age increased, the positive rate of continuity (Co) increased. There were statistically significant differences in Co positive rate of aEEG among premature infants of different gestational ages (P<0.05). As gestational age increased, the positive rate of sleep-wakefulness cycle (SWC) increased. There were statistically significant differences in SWC positive rate of aEEG among premature infants of different gestational ages (P<0.05). As gestational age increased, lower-bound (LB) amplitude increased. There were statistically significant differences in LB of aEEG < 3μV and aEEG >5μV among premature infants of different gestational ages (P<0.05). As the gestational age increased, bandwidth (B) decreased, and there were statistically significant differences in B of aEEG among premature infants of different gestational ages (P<0.05). There were statistically significant differences in fractional anisotropy (FA) values of frontal white matter, parietal white matter, splenium of the corpus callosum, and cerebral peduncle among premature infants at different gestational ages (P<0.05). Spearman correlation analysis results showed that LB was negatively correlated with FA values of the splenium of the corpus callosum and cerebral peduncle. B was positively correlated with FA value of parietal white matter, and negatively correlated with FA value of cerebral peduncle (P<0.05). Conclusion: With the increase of gestational age, aEEG becomes mature, which mainly manifests as increases in Co and SWC positive rates, increase in LB and decrease in B. Closely related to brain development in premature infants. 2025 Vol. 31 (6): 954-958 [Abstract] ( 7 ) HTML (1 KB)  PDF (1641 KB)  ( 2 ) 959 Effects of Conventional Drug Therapy Combined with Low-Frequency Repetitive Transcranial Magnetic Stimulation on Serum PGP MRP1 Protein and EEG in Patients with Refractory Epilepsy YU Wenping, CHEN Fenfen, et al DOI: 10.3969/j.issn.1006-6233.2025.06.15 Objective: To investigate the effect of low frequency repetitive transcranial magnetic stimulation (lfms) on drug-resistant protein and electroencephalogram (EEG) in patients with intractable epilepsy. Methods: A total of 60 patients with intractable epilepsy from January 2023 to December 2023 in our hospital. The observation group was treated with low frequency repetitive transcranial magnetic stimulation (lfms) plus conventional antiepileptic drugs, while the control group was only treated with conventional antiepileptic drugs. The serum levels of p-glycoprotein (PGP) and multidrug resistance-associated protein 1(MRP1) were measured before and after 12 weeks of treatment, the clinical effect and the improvement of electroencephalogram were evaluated. Results: After 12 weeks of treatment, the levels of serum PGP and MRP1 protein in the observation group were significantly decreased, the number of attacks were significantly reduced, and the electroencephalogram discharge index was significantly decreased (P<0.05). The incidence of adverse reactions in the observation group was lower than that in the control group (P<0.05). The difference in the number of seizures between the two groups before and after treatment was statistically significant (P<0.05). There was a statistically significant difference in PGP serum levels between the two groups before and after treatment (P<0.05), while there was no statistically significant difference in MRP1 serum levels (P>0.05), indicating a greater impact on PGP and a smaller impact on MRP1. The improvement rate of electroencephalogram in the rTMS group was 80.0%, significantly higher than the 46.67% in the control group (P<0.05). Conclusion: Low-frequency repetitive transcranial magnetic stimulation combined with antiepileptic drugs can reduce the levels of serum PGP and MRP1 protein in patients with intractable epilepsy and reduce the number of seizures, and the treatment is relatively safe. 2025 Vol. 31 (6): 959-963 [Abstract] ( 6 ) HTML (1 KB)  PDF (1197 KB)  ( 1 ) 963 Analysis of Pathogenetic Features Drug Resistance and Risk Factors of Fungal Infections Complicating COPD SUN Liying, JIANG Qi, PAN Ni, et al DOI: 10.3969/j.issn.1006-6233.2025.06.16 Objective: To investigate the pathogenic features, drug resistance and risk factors for fungal infections in patients with chronic obstructive pulmonary disease (COPD). Methods: The etiology and clinical data of COPD patients who were treated in our hospital from March 2022 to March 2024 were selected and analyzed. Results: A total of 84 strains of pathogenic bacteria were isolated from 62 patients with COPD complicated with fungal infection, including Candida albicans (41.67%), Candida tropicalis (22.62%) and Aspergillus fumigatus (14.29%). Pathogenic bacteria had good sensitivity to voriconazole and fluconazole. Age, course of disease, hypoproteinemia, hormone use time and invasive operation were independent influencing factors of fungal infection complicating COPD patients. Conclusion: The invasive strains of COPD patients with fungal infection are extensive and have certain drug resistance. The occurrence of infection is related to age, course of disease, hypoproteinemia, hormone use time and invasive operation. Paying attention to the pathogenic characteristics, drug resistance and risk factors of fungal infection, and taking timely intervention measures can help improve the quality of life of COPD patients. 2025 Vol. 31 (6): 963-968 [Abstract] ( 6 ) HTML (1 KB)  PDF (1201 KB)  ( 1 ) 969 Study on the Relationship and Clinical Value of Prognostic Regression of Paediatric Severe Pneumonia with MPV/PLT and Inflammatory Response Related Indexes ZHOU Beibei, LI Jie, ZHANG Meng DOI: 10.3969/j.issn.1006-6233.2025.06.17 Objective: To analyze the correlation of prognosis outcome of children with severe pneumonia (SP), with mean platelet volume (MPV)/platelet count (PLT) and inflammatory response-related indicators, and evaluate its predictive value on prognosis outcome in children with SP. Methods: Totally 115 children with SP in the hospital were selected from July 2022 to July 2024 as the study subjects. According to the prognosis outcome status, the patients were divided into good prognosis group (n=87) and poor prognosis (n=28) group. The levels of MPV/PLT, lactate dehydrogenase (LDH), procalcitonin (PCT) and C-reactive protein (CRP) were measured among all study subjects. Logistic correlation analysis was applied to analyze the association between MPV/PLT, LDH, PCT and CRP levels and prognosis outcome of SP children. The predictive value of MPV/PLT, LDH, PCT and CRP on the prognosis outcomes of SP in children was analyzed by receiver operating characteristic curve (ROC). Results: The MPV/PLT, LDH, PCT and CRP in the poor prognosis group were higher than those in the good prognosis group (P<0.05). Logistic correlation analysis demonstrated that MPV/PLT, LDH, PCT and CRP levels were closely correlated with the prognosis outcome of SP children (P<0.05). ROC curve indicated that the area under the curve (AUC), sensitivity and specificity of MPV/PLT, LDH, PCT and CRP in predicting the prognosis outcome were 0.953, 83.33% and 97.01%. The efficiency of combined prediction was better than that of each indicator alone (P<0.05). Conclusion: The prognosis outcome of SP in children is closely related to MPV/PLT and inflammatory response-related indicators. Early determination of MPV/PLT, LDH, PCT and CRP levels can provide a certain reference for clinical prediction of prognosis outcome of children with SP. 2025 Vol. 31 (6): 969-973 [Abstract] ( 7 ) HTML (1 KB)  PDF (1379 KB)  ( 1 ) 973 Correlation of Peripheral Blood miR-372-3p and miR-27a Levels with Insulin Resistance and Neonatal Outcome in Pregnant Women with Gestational Diabetes Mellitus MA Qianqian, MA Dan, CHEN Dongying, et al DOI: 10.3969/j.issn.1006-6233.2025.06.18 Objective: To observe the association of changes of peripheral blood short-chain non-coding RNA (miRNA)-372-3p and miR-27a with insulin resistance (IR) and neonatal outcomes in patients with gestational diabetes mellitus (GDM). Methods: 102 patients with GDM in the hospital were included from June 2022 to April 2024, and 102 normal pregnant women were selected for control. The levels of miR-372-3p and miR-27a in peripheral blood of the enrolled patients were measured, and the patients were divided into normal outcome group and adverse outcome group according to the neonatal outcomes. The general data and detection information of patients were collected, and ROC curve was drawn to analyze the predictive value of miR-372-3p, miR-27a and insulin resistance on neonatal adverse outcomes of pregnant women with GDM. Results: The peripheral blood miR-372-3p, miR-27a and IR index in GDM group were significantly higher than those in normal pregnant women group the difference was statistically significant (P<0.05). Pearson correlation analysis indicated that the expression levels of miR-372-3p and miR-27a in GDM patients were positively correlated with IR index, and the correlations were statistically significant (r=0.518, 0.606, P<0.05). Linear regression analysis demonstrated that age, body mass index and expression levels of miR-372-3p and miR-27a were influencing factors for IR index in GDM pregnant women, and the associations were statistically significant (P<0.05). The expression levels of miR-372-3p and miR-27a and IR index of GDM pregnant women in neonatal adverse outcome group were higher than those in normal outcome group, and there were statistical differences (P<0.05). ROC curve showed that the AUCs of miR-372-3p, miR-27a and IR in predicting neonatal outcomes of GDM pregnant women were 0.740, 0.806 and 0.669 respectively. Conclusion: The increased expressions of miR-372-3p and miR-27a in peripheral blood of GDM patients promote the risk of IR, and have certain application value on evaluating neonatal outcomes. 2025 Vol. 31 (6): 973-978 [Abstract] ( 6 ) HTML (1 KB)  PDF (1268 KB)  ( 1 ) 978 Effect of Steel Wire Tension Band Fixation Combined with Total Hip Arthroplasty in the Treatment of Elderly Osteoporotic Femoral Intertrochanteric Fractures ZHANG Youshu, DING Wenxing, DONG Yao, et al DOI: 10.3969/j.issn.1006-6233.2025.06.19 Objective: To explore the efficacy of steel wire tension band fixation combined with total hip arthroplasty (THA) in the treatment of elderly osteoporotic femoral intertrochanteric fractures. Methods: From January 2020 to January 2023, 102 elderly patients with osteoporotic femoral intertrochanteric fractures were randomly divided into THA treatment group and combined treatment group by lottery method, with 51 cases in each group. The THA treatment group was treated with THA, whereas the combined treatment group was given steel wire tension band fixation combined with THA. The surgical evaluation parameters (intraoperative blood loss, surgical time, bed rest time and fracture healing time), hip ranges of motions, clinical efficacy (Harris hip score) and complications were compared between groups of patients. Results: Compared with THA treatment group, the surgical time in combined treatment group was longer, and the bed rest time and fracture healing time were shortened, and the intraoperative blood loss was reduced, and the incidence rates of complications were decreased (P<0.05). At 4 months after surgery, the excellent and good rate of clinical efficacy in combined treatment group was higher than that in THA treatment group (P<0.05). The hip ranges of motions in the two groups were enlarged, and the differences of hip ranges of motions at 4 months after surgery and before surgery were greater in combined treatment than those in THA treatment group (P<0.05). Conclusion: Although steel wire tension band fixation combined with THA in the treatment of elderly osteoporotic femoral intertrochanteric fractures increases the surgical time of THA, it can effectively shorten the bed rest time and fracture healing time, reduce the intraoperative blood loss, increase the hip ranges of motions, and enhance the clinical efficacy, and has high safety. 2025 Vol. 31 (6): 978-982 [Abstract] ( 6 ) HTML (1 KB)  PDF (1205 KB)  ( 1 ) 983 Comparison of Glass-ceramic and High-permeability Zirconia All-ceramic Crowns Restorations in Patients with Anterior Tooth Defects CHEN Gang, et al DOI: 10.3969/j.issn.1006-6233.2025.06.20 Objective: To analyze the impact of restoration using glass-ceramic all-ceramic crowns and high-permeability zirconia all-ceramic crowns on aesthetic effect, masticatory function and 1-year follow-up results in patients with anterior tooth defect. Methods: From November 2022 to November 2023, 156 patients with anterior tooth defects admitted to the hospital were selected and randomly assigned to the control group (n=78, 98 prostheses) and the observation group (n=78, 92 prostheses). The control group underwent restoration using high-permeability zirconia all-ceramic crowns, while the observation group underwent restoration using glass-ceramic all-ceramic crowns. Both groups were followed up for one year. The two groups were compared in terms of aesthetic effect [Pink Esthetic Score (PES) and White Esthetic Score (WES)], masticatory function, periodontal indexes [probing depth (PD), bleeding index (BI) and plaque index (PLI)], and the incidence of adverse events. Results: The difference values of PES and WES scores before and after restoration were higher in the observation group than in the control group (P<0.05). Comparisons of difference values of bite force, masticatory efficiency, PD, BI and PLI scores before and after restoration found no statistically significant differences between the two groups (P>0.05). The incidence of adverse events was higher in the observation group than in the control group (P<0.05). Conclusion: Restoration using glass-ceramic all-ceramic crowns and high-permeability zirconia all-ceramic crowns both can improve masticatory function and periodontal indexes in patients with anterior tooth defects. However, the former can achieve better aesthetic effects, and the incidence of adverse events after restoration using high-permeability zirconia all-ceramic crowns is lower. 2025 Vol. 31 (6): 983-987 [Abstract] ( 6 ) HTML (1 KB)  PDF (1202 KB)  ( 1 ) 987 The Effect of Zirconia Post plus Zirconia Crown and Fiber Post plus Zirconia Crown in Repairing Large Area Tooth Defects CHU Qinqin, PAN Baosheng, ZHANG Hui, et al DOI: 10.3969/j.issn.1006-6233.2025.06.21 Objective: To investigate the effect of zirconia post plus zirconia crown and fiber post plus zirconia crown in repairing large area tooth defects. Methods: Totally 117 patients who received large-area tooth defect restoration in the hospital from April 2020 to April 2024 were included. With the random number table method, the patients were divided into the zirconia post group (n=61) and the fiber post group (n=56). The zirconia post group received zirconia post plus zirconia crown restoration, and the fiber post group underwent fiber post plus zirconia crown restoration. The patients were followed up for 6 months. The masticatory function (occlusal force, masticatory efficiency), restoration stability (PTV value), restoration time efficiency, gingival health [gingival bleeding index (BI), periodontal pocket depth (PD), gingival index (GI)], gingival crevicular fluid inflammatory factors [interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), prostaglandinE2 (PGE2)], restoration shedding status, edge tightness and aesthetic effect [pink esthetic score (PES), white esthetic score (WES)] were compared between groups before restoration and at 6 months after restoration. Results: After 6 months of restoration, the masticatory function in the two groups was significantly improved, and the differences of occlusal force and masticatory efficiency before and after restoration in the zirconia post group were higher than those in the fiber post group (P<0.05). The total restoration time was (12.00±3.50) days in the fiber post group and (27.00±7.50) days in the zirconia post group, and the time efficiency in the fiber post group was better than that in the zirconia post group (t=13.660, P<0.001). The stability of restorations in both groups was increased, and the difference of PTV value before and after restoration was higher in the zirconia post group than that in the fiber post group (P<0.05). The periodontal health status was improved in both groups at 6 months after restoration, and the differences of BI, PD and GI before and after restoration were greater in the fiber post group compared to the zirconia post group (P<0.05). The differences of IL-1β, TNF-α and PGE2 before and after restoration in the fiber post group were greater compared with those in the zirconia post group (P<0.05). After 6 months of restoration, there were no statistical differences in the restoration shedding status and edge tightness between both groups (P>0.05). The differences of PES score and WES score before and after restoration in the fiber post group after 6 months of restoration were higher compared to the zirconia post group (P<0.05). Conclusion: Fiber post restoration has better performance in terms of time efficiency, aesthetic effect and periodontal health improvement, and zirconia post restoration has more advantages in masticatory efficiency and stability. 2025 Vol. 31 (6): 987-993 [Abstract] ( 6 ) HTML (1 KB)  PDF (1251 KB)  ( 1 ) 993 Analysis of Risk Factors Affecting Maxillofacial Gap Infection after Oral Trauma SHEN Da, LI Jian, ZUO Wei DOI: 10.3969/j.issn.1006-6233.2025.06.22 Objective: To analyze the risk factors affecting maxillofacial gap infection after oral trauma. Methods: The clinical data of 115 patients with maxillofacial gap infection after oral trauma in the hospital from January 2020 to January 2024 were retrospectively analyzed and the patients were included in infection group. The clinical data of 123 patients with non-infection after oral trauma who were treated in the hospital during the same period were retrospectively analyzed and the patients were set as non-infection group. The general data such as gender, age, history of diabetes mellitus, iatrogenic procedure, hormone use and nutritional status, serum inflammatory indexes [D-dimer (D-D), erythrocyte sedimentation rate (ESR), macrophage inflammatory protein-2 (MIP-2), serum amyloid A (SAA), C-reactive protein (CRP), soluble cluster of differentiation 14 subtype (sCD14-ST)] and blood routine indexes [white blood cell count, platelet count, neutrophil count (NEU)] at admission were compared between groups. The risk factors of maxillofacial space infection after oral trauma were analyzed by logistic regression analysis. Results: nivariate analysis showed that age, history of diabetes mellitus, iatrogenic improper procedure, proportion of nutritional status, MIP-2, CRP, sCD14-ST, white blood cell count and NEU had statistically significant differences (P<0.05). After logistic regression analysis, it was found that age>60 years old, history of diabetes mellitus, iatrogenic improper procedure, poor nutritional status and increased MIP-2, CRP, sCD14-ST, white blood cell count and NEU were independent risk factors for maxillofacial space infection after oral trauma (P<0.05). Conclusion: Age>60 years old, concomitant diabetes mellitus, iatrogenic improper procedure, poor nutritional status, MIP-2, CRP, sCD14-ST, white blood cell count and NEU are independent risk factors affecting maxillofacial gap infection after oral trauma 2025 Vol. 31 (6): 993-997 [Abstract] ( 7 ) HTML (1 KB)  PDF (1196 KB)  ( 3 ) 998 The Effect of Curcumin in Rats with Apical Periodontitis THROUGH the AhR/NF-κB/NLRP3 Signaling Pathway CHEN Bei, ZHANG Jianfen, LIU Yile, et al DOI: 10.3969/j.issn.1006-6233.2025.06.23 Objective: To investigate the effect of curcumin on inflammation and bone resorption in rats with apical periodontitis (AP) through the AhR/NF-κB/NLRP3 signaling pathway. Methods: The SD rats were divided into a control group, a model group, a curcumin group, and a curcumin + AhR antagonist CH-223191 group, each comprising 12 individuals. The first molar pulp was opened to construct an AP rat model. The control group (normal saline), model group (normal saline), curcumin group (100mg·kg-1·d-1 for gavage), and curcumin + CH-223191 group (100mg·kg-1·d-1 for gavage, 5mg·kg-1·d-1 for intraperitoneal injection) were continuously intervened for 4 weeks. The periapical tissues were scanned to assess their destruction by Micro-CT. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect the number of osteoclasts in periapical tissues. Serum TNF-α, IL-6, IL-1β levels were detected by ELISA assay. HE staining was used to detect the infiltration of inflammatory cells in rat periapical tissues. The expression of osteoprotectin (OPG), nuclear factor κB receptor activator ligand (RANKL), AhR, p-p65 NF-κB/p65 NF-κB, NLRP3, caspase-1 p20, GSDMD proteins in periapical tissue was detected by Western blot. Results: Compared with the control group, the periapical tissue of the model group showed obvious bone resorption, the number of osteoclasts in periapical tissues significantly increased (P<0.05), the serum levels of TNF-α, IL-6, IL-1β significantly increased (P<0.05), the number of inflammatory cells in the periapical tissues increased, the expressions of OPG and AhR proteins in periapical tissues significantly down-regulated (P<0.05), the expression of RANKL, p-p65 NF-κB/p65 NF-κB, NLRP3, caspase-1 p20, GSDMD proteins in periapical tissues significantly up-regulated (P<0.05); Compared with the model group, the bone resorption of periapical tissues in the curcumin group reduced, the number of osteoclasts in periapical tissues significantly decreased (P<0.05), the serum levels of TNF-α, IL-6, IL-1β significantly decreased (P<0.05), the number of inflammatory cells in the periapical tissues decreased, the expressions of OPG and AhR proteins in periapical tissues significantly up-regulated (P<0.05), the expression of RANKL, p-p65 NF-κB/p65 NF-κB, NLRP3, caspase-1 p20, GSDMD proteins in periapical tissues significantly down-regulated (P<0.05); Compared with the curcumin group, the above indexes in the curcumin+CH-223191 group were significantly reversed (P<0.05). Conclusion: Curcumin reduces bone resorption in periapical tissues of AP rats by inhibiting inflammation, and its mechanism may be related to regulating the AhR/NF-κB/NLRP3 signaling pathway. 2025 Vol. 31 (6): 998-1003 [Abstract] ( 6 ) HTML (1 KB)  PDF (1824 KB)  ( 1 ) 1003 Mechanistic Study of LINC00511 Regulating PGK1 in a Mouse Model of High-Risk HPV-Positive Cervical Cancer WANG Lijing, TIAN Miao, CAO Jiting, et al DOI: 10.3969/j.issn.1006-6233.2025.06.24 Objective: To investigate the expression profile of long non-coding RNA (lncRNA) LINC00511 in cervical cancer induced by high-risk human papillomavirus (HPV) and deeply analyze its molecular mechanism of promoting cervical cancer progression by regulating Phosphoglycerate Kinase 1 (PGK1), as well as evaluate the feasibility of LINC00511 as a potential therapeutic target. Methods: A mouse model of cervical cancer infected with high-risk HPV16 was established, and the experimental animals were divided into a control group and a LINC00511 knockdown group (si-LINC00511 group). qRT-PCR was used to detect the mRNA expression levels of LINC00511 and PGK1 in mouse tumor tissues, and Western blot was performed to detect the protein expression and phosphorylation level of PGK1. Immunohistochemical analysis was used to examine the expression of PGK1 and the proliferation marker Ki-67 in tumor tissues. Tumor volume and weight were measured, and tumor cell apoptosis was evaluated. The target regulatory relationship between LINC00511 and PGK1 was verified. Results: The expression level of LINC00511 in cervical cancer tissues of HPV16-infected mice was significantly higher than that in the normal control group (P<0.001). The mRNA and protein expression levels of PGK1 were significantly upregulated (P<0.01). In the tumor tissues of LINC00511-knockdown mice, the mRNA and protein expression levels of PGK1 were significantly downregulated (P<0.01), and the phosphorylation level of PGK1 was also significantly reduced, indicating that LINC00511 positively regulates PGK1 expression and activity. The tumor volume in the si-LINC00511 group was 562.3±43.5mm3, compared with 825.1±67.2 mm3 in the control group (P<0.001). The tumor weight was 0.48±0.05 g in the si-LINC00511 group and 0.79±0.06 g in the control group (P<0.001). Immunohistochemical analysis showed that the proportion of Ki-67-positive cells in the tumor tissues of LINC00511-knockdown mice was significantly reduced (P<0.01), indicating that tumor cell proliferation was inhibited. TUNEL assay showed a significant increase in the proportion of apoptotic positive cells (P<0.01), suggesting that LINC00511 knockdown promotes tumor cell apoptosis. Dual-luciferase reporter gene assay showed that LINC00511 positively regulates PGK1 expression by binding to the 3'UTR of PGK1 mRNA. Conclusion: LINC00511 is significantly overexpressed in cervical cancer associated with high-risk HPV16 infection and promotes tumor cell proliferation while inhibiting apoptosis by upregulating PGK1 expression. Knockdown of LINC00511 effectively reduces PGK1 expression and slows tumor growth, suggesting that the LINC00511-PGK1 axis plays a crucial role in the development and progression of high-risk HPV-related cervical cancer. 2025 Vol. 31 (6): 1003-1010 [Abstract] ( 7 ) HTML (1 KB)  PDF (2191 KB)  ( 3 ) 1010 Comparison of Short-Term Efficacy and Aesthetics of Breast Tumor Plastic Breast-Conserving Surgery and Extended Resection Breast-Conserving Surgery in the Treatment of Early Breast Cancer ZHANG Yi, CHEN Yao, WANG Jing DOI: 10.3969/j.issn.1006-6233.2025.06.25 Objective: To compare the influence of breast tumor plastic breast-conserving surgery and extended resection breast-conserving surgery on short-term efficacy and wound aesthetics in patients with early breast cancer. Methods: The clinical data of patients with early breast cancer in the hospital were retrospectively analyzed from January 2021 to January 2024. Fourty-six effective cases were randomly selected from patients undergoing breast tumor plastic breast-conserving surgery and included in group A, and another fourty-six effective cases were randomly collected from patients with extended resection breast-conserving surgery and enrolled as group B. The surgical indicators, short-term efficacy and incidence rates of postoperative complications were compared between groups. The breast aesthetics at 6 months after surgery and levels of tumor serum markers [CA153, heat shock protein 90 A (HSP90 A), osteopontin (OPN), secretory leukocyte protease inhibitor (SLPI)] and quality of life [Functional Assessment of Cancer Therapy-Breast (FACT-B)] before surgery and at 6 months after surgery were recorded. Results: The surgical time, intraoperative blood loss and intraoperative drainage volume in group A were significantly shorter or less than those in group B, and the incision length was significantly shorter than that in group B (all P<0.05). At 6 months after surgery, there was no statistical significance in objective response rate (ORR) between groups (P>0.05). The breast aesthetic rate in group A (58.70%) was significantly higher than that in group B (36.96%) (P<0.05). The levels of serum tumor markers in the two groups were lower than those before surgery (P<0.05), but there was no statistical difference between groups (P>0.05). At 6 months after surgery, the FACT-B scores were higher in both groups than before surgery, and group A had higher scores than group B (all P<0.05). The total incidence rate of complications in group A (19.57%) was lower than that in group B (39.13%) (P<0.05). Conclusion: Breast tumor plastic breast-conserving surgery and extended resection breast-conserving surgery have similar efficacy, but plastic breast-conserving surgery can more effectively improve postoperative breast aesthetics and quality of life of patients, and reduce the incidence rates of postoperative complications. It is suggested to choose the appropriate surgical method for treatment based on the actual situation and the needs of patients in clinical application. 2025 Vol. 31 (6): 1010-1016 [Abstract] ( 7 ) HTML (1 KB)  PDF (1235 KB)  ( 2 ) 1016 Pelvic Floor Myofascial Manipulation Combined with Pelvic Floor Muscle Electrical Stimulation in Patients with Postpartum Mild Uterine Prolapse WANG Hui, CHEN Suhui, BO Xueping, et al DOI: 10.3969/j.issn.1006-6233.2025.06.26 Objective: To explore the clinical effect of pelvic floor myofascial manipulation combined with pelvic floor muscle electrical stimulation on patients with postpartum mild uterine prolapse. Methods: Totally 102 patients with mild postpartum uterine prolapse seen in our hospital from June 2023 to June 2024 were retrospectively selected and divided into two groups according to the different intervention programmes adopted, 51 cases in the control group received pelvic floor electromyographic stimulation treatment, and 51 cases in the combined group received combined pelvic floor myofascial manipulation treatment. The two groups were compared in terms of the degree of pelvic organ prolapse quantitative staging method (POP-Q), the pelvic floor surface electromyographicGlazer score, clinical efficacy, pain level [Visual Analogue Scoring (VAS)] score, adverse reactions, and long-term efficacy [results of pelvic floor muscle strength grading assessment]. Results: After the treatment, the degree of grade II in the POP-Q of the combined group was lower than that of the control group (P<0.05). The changes of pre-resting score, post-resting score and VAS score before and after treatment in the combined group with (9.40±1.22) points, (7.62±1.52) points and (1.98±0.34) points were higher compared with (4.20±0.76) points, (2.22±0.61) points and (0.67±0.15) points in the control group (P<0.05). After treatment, the total effective rate of the combined group was 92.16% higher than that of the control group at 76.47% (P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05). After a 6-month follow-up, the pelvic floor muscle strength grading assessment grade 5 in the combined group was higher than that in the control group (P<0.05). Conclusion: Combination of pelvic floor myofascial manipulation on the basis of pelvic floor muscle electrical stimulation can enhance the pelvic floor muscle strength of patients with mild uterine prolapse and improve the degree of uterine prolapse. It has a certain effect on relieving pain and has significant efficacy. 2025 Vol. 31 (6): 1016-1021 [Abstract] ( 6 ) HTML (1 KB)  PDF (1211 KB)  ( 2 ) 1022 The Relationship of Multifidus Muscle Fat Ratio and Cross-Sectional Area with Pfirrmann Grading and Intervertebral Disc Herniation Reabsorption WANG Feng, DAI Guogang, DU Wanli, et al DOI: 10.3969/j.issn.1006-6233.2025.06.27 Objective: To explore and analyze the association of multifidus muscle fat ratio and cross-sectional area changes with pfirrmann grading and reabsorption of intervertebral disc nucleus pulposus after treatment in patients with lumbar disc herniation (LDH). Methods: Totally 105 patients with LDH admitted to the hospital from July 2020 to July 2023 were included. The changes of cross-sectional area and fat ratio of multifidus muscle were detected by magnetic resonance imaging (MRI), and the relationship with pfirrmann grading and reabsorption of intervertebral disc nucleus pulposus after treatment was analyzed. Results: There were statistical differences in the proportion of affected side, the muscle area of healthy L5/S1-pre pure multifidus muscle and healthy L5/S1-pre fat ratio between LDH patients with pre-pfirrmann grade 3 and grade 4 (P<0.05). The differences in age and affected L4/L5-post fat ratio among LDH patients with post-pfirrmann grade 3, grade 4 and grade 5 were statistically significant (P<0.05). The proportion of post-pfirrmann grading, affected/healthy L4/L5-post fat ratio and affected/healthy L5/S1-post fat ratio revealed no statistical differences between patients with reabsorption and without reabsorption (P<0.05). Logistic regression showed that the ratio of healthy L4/L5-post fat was a risk factor for reabsorption after treatment (P<0.05). ROC curves showed that the AUC of LDH reabsorption after LDH treatment as assessed by healthy L4/L5-post fat ratio was 0.729, respectively. Conclusion: The muscle area of healthy L5/S1-pre pure multifidus muscle and the fat proportion of healthy L5/S1-pre were different in different pre-pfirrmann grades, while the fat proportion of patients with L4/L5-post was different in different post-pfirrmann grades.Moreover, the healthy L4/L5-post fat ratio is related to reabsorption after LDH treatment, and has a certain evaluation value for reabsorption. 2025 Vol. 31 (6): 1022-1028 [Abstract] ( 6 ) HTML (1 KB)  PDF (1261 KB)  ( 2 ) 1028 The Spatial Osteogenic Effect of Using Autologous Bone Fragment in Simultaneous Implantation during Maxillary Sinus External Elevation Surgery KONG Lingbing, DONG Shitao, FAN Jiamei, et al DOI: 10.3969/j.issn.1006-6233.2025.06.28 Objective: To investigate the spatial osteogenic effect of autologous bone slices in the simultaneous implantation of maxillary sinus elevation. Methods: Sixty patients who underwent dental implant restoration in the maxillary posterior region at Chengde Stomatological Hospital from May 2023 to January 2024 were selected and randomly divided into a study group and a control group, with 30 patients in each group. In the study group, 112 implants were implanted after the ultrasonic bone knife was used to open the anterolateral wall of the maxillary sinus and the open autogenous bone fragments combined with platelet-rich fibrin (PRF) were placed on the floor of the maxillary sinus; in the control group, 104 implants were implanted after the PRF was placed on the floor of the maxillary sinus after opening the anterolateral wall of the maxillary sinus using abrasion.A total of 216 implants were implanted, and pain, osteogenesis and healing were analysed and evaluated in both groups at 1, 6 and 12 months after surgery. Results: Both the study group and the control group achieved good results of maxillary sinus floor elevation, but in the control group, the height of bone increment tended to decrease more significantly after 6 months postoperatively, and although the elevation purpose could be achieved, there was more resorption of new bone. Conclusion: In patients with insufficient bone volume in the maxillary posterior region, autogenous bone fragments combined with PRF applied in maxillary sinus external elevation and implant placement at the same time can achieve more stable bone gain effect. 2025 Vol. 31 (6): 1028-1033 [Abstract] ( 6 ) HTML (1 KB)  PDF (2207 KB)  ( 2 ) 1033 Prediction of Cardiac Autonomic Neuropathy in T2DM by Constructing a Column-Linear Graphical Model Based on Lorenz Scatterplot and Three-Dimensional RR Scatterplot Heart Rate Variability LI Chunyan, ZHONG Ye, CHAI Juanjuan, et al DOI: 10.3969/j.issn.1006-6233.2025.06.29 Objective: To investigate the predictive value of heart rate variability (HRV)-constructed column-linear graphical models based on Lorenz scatterplot and three-dimensional RR scatterplot for cardiac autonomic neuropathy (DCAN) in type 2 diabetes mellitus (T2DM). Methods: Totally 103 patients with T2DM admitted between April 2021 and March 2024 were selected for retrospective analysis, all patients underwent Lorenz scatterplot and 3D RR scatterplot analyses, patients were divided into DCAN and NDCAN groups based on cardiovascular reflexology test (CART), and the differences in HRV indices between the two groups were compared, and the correlation between scatterplot HRV indices and time domain indices was analysedWe constructed a column-line graph model of scatterplot HRV indexes by R software, and evaluated the predictive value of the model for the occurrence of DCAN in T2DM patients. Results: The standard deviation of 24-h normal sinus intervals (SDNN), long axis (L0), short axis (W), long-short axis ratio (L0/W) of Lorenz scatter plot, long axis of three-dimensional RR scatter plot in stereospace (L), long axis of two-dimensional graph in the xoy plane (L1), and long axis on the x-axis of the quadratic projection (L2) of DCAN group were lower than that of NDCAN group, and the difference was statistically significant (P<0.05); Pearson correlation analysis showed that L0, W, L, L1, L2 were positively correlated with SDNN (r=0.933, 0.227, 0.272, 0.931, 0.932, P<0.001), and the correlation between L0/W and SDNN was not statistically significant (r=0.054, P=0.586); and multifactorial analysis showed that L0, L1,L2 were independent influences on the occurrence of DCAN in T2DM patients (P<0.05); a 3-factor predictive model Ln(P/1-P)=11.127-0.005*L0-0.003*L1-0.010*L2 was established for the combined application, and the Hosmer-Lemeshow goodness-of-fit test was performed on the model, with P=0.842>0.05, theThe area under the ROC curve was calculated to be 0.942 with a 95% CI of 0.899 to 0.985, and the decision curves showed that the net patient benefit was higher in both cases than in the other two extreme curves, and that the model yielded a better clinical benefit with a threshold probability of 0.02 to 0.92. Conclusion: The column-linear graphical model constructed on the basis of Lorenz scatterplot and three-dimensional RR scatterplot HRV indicators (L0, L0/W, L1) has a high predictive value for the occurrence of DCAN in patients with T2DM, and the model can help to identify patients at high risk of DCAN at an early stage. 2025 Vol. 31 (6): 1033-1038 [Abstract] ( 7 ) HTML (1 KB)  PDF (1767 KB)  ( 2 ) 1039 The Application Value of Lateral Position Direct Anterior Approach Total Hip Arthroplasty in Developmental Dysplasia of the Hip Joint ZHANG Lichao, WU Di, et al DOI: 10.3969/j.issn.1006-6233.2025.06.30 Objective: To investigate the efficacy of direct anterior approach total hip arthroplasty in the treatment of developmental dysplasia of the hip in the lateral decubitus position and to observe changes in postoperative spinal-pelvic X-ray images. Methods: A total of 82 patients with DDH who underwent total hip arthroplasty in the Department of Joint Surgery of the Affiliated Hospital of Chengde Medical University from June 2015 to June 2022 were retrospectively analyzed. According to the different surgical approaches, they were divided into a direct anterior approach group and a posterolateral approach group. The age, hip joint classification, gender, affected side, incision length, operation time, hospital stay, postoperative drainage volume, and complications of the two groups were compared. The Harris hip score and visual analogue scale (VAS) score of patients in the direct anterior approach group and the posterolateral approach group were recorded preoperatively, 3d,14d postoperatively, and 1, 3,12and 24 months postoperatively, and the hip abduction and flexion angles were measured. The changes of sacrum tilt angle, iliac tilt angle, and hip joint tilt angle were measured on standing position X-ray film before and 1 month after operation. Results: There were statistically significant differences in incision length, operation time, hospital stay, postoperative drainage volume, and complications between the two groups (P<0.05), and DAA group had better results than PLA group. The changes of hip Harris score, VAS score, hip abduction and flexion angle in the direct anterior approach group and posterolateral approach group at different time points before and after operation were statistically significant (P<0.05). The hip Harris score, hip abduction and flexion angle in the two groups after operation were significantly increased compared with those before operation, and the postoperative VAS score was significantly decreased compared with those before operation. After total hip arthroplasty, the sacral tilt angle, iliac tilt angle and hip joint tilt angle of the two groups were significantly lower than those before operation (P<0.001). There was no significant difference in Harris score, VAS score, hip abduction, and flexion angle between the direct anterior approach group and the anterolateral approach group at different time points before and after operation (P > 0.05). Conclusion: The direct anterior approach total hip arthroplasty in the lateral decubitus position demonstrates significant efficacy in treating developmental dysplasia of the hip and improves pelvic tilt in patients with developmental dysplasia of the hip. 2025 Vol. 31 (6): 1039-1043 [Abstract] ( 8 ) HTML (1 KB)  PDF (1201 KB)  ( 1 ) 1043 Influence of Tirofiban Combined with PCI on Efficacy PCI-Related Myocardial Infarction and Prognosis in Patients with Acute Coronary Syndrome LIU Yuebin, LIU Hong, ZHOU Jinfeng DOI: 10.3969/j.issn.1006-6233.2025.06.31 Objective: To explore the influence of intracoronary application of tirofiban on percutaneous coronary intervention (PCI)-related myocardial infarction (PMI) and clinical prognosis in treating acute coronary syndrome (ACS) after PCI. Methods: Totally 106 patients with ACS in the hospital from January 2020 to July 2024 were retrospectively selected and treated with PCI. According to whether tirofiban was injected into the coronary artery during surgery, the patients were divided into two groups, with 56 patients in the control group not injected with tirofiban, and 50 patients in the observation group given intracoronary injection of tirofiban. The thrombolysis in myocardial infarction (TIMI) blood flow grading status, myocardial injury markers [creatine kinase isoenzyme (CKMB), cardiac troponin I (cTnI)], postoperative ST segment recovery status, incidence of PMI, short-term cardiac function indicators [left ventricular end-diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF)] and incidence of major adverse cardiovascular events (MACE) were compared and analyzed in both groups. Results: The rate of TIMI blood flow grade 3 in the observation group after surgery was 96.00%, which was higher than 75.00% in the control group (P<0.05). The changes of CKMB and cTnI before and after surgery in the observation group with (11.87±1.48) μg/ml and (2.34±0.25) μg/ml were higher than (10.36±1.33) μg/ml and (1.76±0.33) μg/ml in the control group (P<0.05). At 90 minutes after surgery, the ST segment resolution of (64.28±14.39)% in the observation group was higher compared with (40.31±11.32)% in the control group (P<0.05). The incidence of PMI was lower in the observation group compared to the control group (0 vs. 7.69%) (P<0.05). The changes of LVEDD and LVEF in the observation group with (10.21±1.37) mm and (12.59±2.66)% before surgery and at 180 days after surgery were higher than the control group at (4.50±0.86) mm and (8.11±2.12)% (P<0.05). There was no statistical significance in the incidence of MACE between the groups (P>0.05). Conclusion: Intracoronary injection of tirofiban during PCI can enhance the postoperative TIMI blood flow grading of ACS patients, relieve the myocardial injury, further improve the ST segment recovery status, reduce the incidence of PMI, and improve the short-term cardiac function. 2025 Vol. 31 (6): 1043-1048 [Abstract] ( 8 ) HTML (1 KB)  PDF (1221 KB)  ( 2 ) 1048 Effectiveness of Dexmedetomidine Combined with Nalbuphine on Patients Undergoing Orthognathic Surgery and Its Impact on the Severity of Postoperative Nausea and Vomiting DAI Weilan, et al DOI: 10.3969/j.issn.1006-6233.2025.06.32 Objective: To explore the efficacy of dexmedetomidine combined with nalbuphine in patients undergoing orthognathic surgery (OGS) and its impact on the severity of postoperative nausea and vomiting (PONV). Methods: Ninety patients who underwent OGS in our hospital from August 2019 to December 2024 were randomly divided into a control group (n=45, receiving postoperative analgesia with sufentanil and nalbuphine) and a study group (n=45, receiving intraoperative infusion of dexmedetomidine and postoperative analgesia with sufentanil and nalbuphine) using a block randomization method. The satisfaction with analgesia, analgesic effect, sleep quality, severity of PONV, and incidence of agitation during the recovery period were compared between the two groups. Results: The overall satisfaction rate of pain relief in the research group (86.67%) was higher than that in the control group (64.44%) (P<0.05). Comparison of time-point, between-group, time-point and between-group interactions for visual analogue scale (VAS) and Pittsburgh Sleep Index (PSQI) scores showed statistical significance (P<0.05). The VAS scores of the study group were lower than those of the control group at 8, 16, 24, and 48 hours after surgery (P<0.05), and the PSQI scores of the study group were lower than those of the control group at 24 and 48 hours after surgery (P<0.05). The total incidence of PONV in the study group (11.11%) was lower than that in the control group (60.00%) within 0-12 hours after surgery. However, the total incidence of PONV in the study group (4.44%, 6.67%) within 12-24 hours and 24-48 hours after surgery was not statistically significant compared to the control group (11.11%, 8.89%) (P<0.05). The incidence of agitation during the awakening period in the study group was 6.67 (3/45), which was lower than the control group at 28.89% (13/45) (P<0.05). Conclusion: The combination of dexmedetomidine and nalbuphine in OGS patients can improve analgesic satisfaction, enhance analgesic efficacy and sleep quality, and reduce the severity of PONV and the incidence of agitation during the recovery period. 2025 Vol. 31 (6): 1048-1053 [Abstract] ( 16 ) HTML (1 KB)  PDF (1323 KB)  ( 2 )

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