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2025 Vol. 31, No. 10 Published: 2025-10-31 1585 Impacts of miR-516b-5p on Proliferation and Invasion of Breast Cancer Cells by Targeting NSUN2 CHENG Xinran, FAN Ya'nan, LIU Jun, et al DOI: 10.3969/j.issn.1006-6233.2025.10.01 Objective: To discuss the impacts of micro non-coding RNA-516b-5p (miR-516b-5p) on the proliferation and invasion of breast cancer (BC) cells by targeting NOP2/Sun RNA methyltransferase 2 (NSUN2). Methods: qRT-PCR experiments were performed to detect the miR-516b-5p and NSUN2 mRNA in BC cancer tissues, adjacent tissues, normal breast epithelial cells (MCF-10A), and BC cells (MCF-7, MDA-MB-231, HCC1937). MCF-7 cells were assigned into NC group, miR-NC group, miR-516b-5p mimic group, sh-NC group, sh-NSUN2 group, miR-516b-5p mimic+pcDNA-NC group, and miR-516b-5p mimic+pcDNA-NSUN2 group. Clone formation and Transwell invasion experiments were performed to detect the proliferation and invasion abilities of MCF-7 cells in each group. Western blot was performed to detect the NSUN2, cyclin 1 (CCND1), cyclin dependent kinase 1 (CDK1), matrix metalloproteinase 2 (MMP2), and matrix metalloproteinase 9 (MMP9) proteins. The nude mouse BC transplant tumor model was constructed and separated into Model group, miR-516b-5p mimic group, and miR-516b-5p mimic+pcDNA-NSUN2 group. The tumor mass and volume were measured in each group. Results: For adjacent tissues, the miR-516b-5p in cancer tissues was unusually declined, while the NSUN2 mRNA was clearly increased (P<0.05). Compared with MCF-10A cells, the miR-516b-5p in MCF-7, MDA-MB-231, and HCC1937 cells declined clearly, while the NSUN2 mRNA raised unusually (P<0.05), with the most unusual trend observed in MCF-7 cells. For the NC group, there were no unusual changes in various cellular indicators in the miR-NC group and sh-NC group (P>0.05). For the miR-NC group, the miR-516b-5p mimic group showed declined the number of colony forming cells and invasive cells, unusually decreased NSUN2 mRNA, and NSUN2, CCND1, CDK1, MMP2, and MMP9 proteins, and clearly raised miR-516b-5p (P<0.05). For the sh-NC group, the changes in various indicators in the sh-NSUN2 group were consistent with those in the miR-516b-5p mimic group (P<0.05). The trend of changes in various indicators in the miR-516b-5p mimic+pcDNA-NSUN2 group was opposite to that in the miR-516b-5p mimic group (P<0.05). The dual luciferase assay confirmed a targeted relationship between miR-516b-5p and NSUN2 (P<0.05). In the nude mouse tumorigenesis experiment, the miR-516b-5p mimics group showed a clear reduction in tumor tissue mass and volume than the Model group (P<0.05). For the miR-516b-5p mimic group, the miR-516b-5p mimic+pcDNA-NSUN2 group showed a prominent increase in tumor tissue mass and volume (P<0.05). Conclusion: MiR-516b-5p can inhibit BC cell proliferation and invasion by targeting and downregulating NSUN2. 2025 Vol. 31 (10): 1585-1592 [Abstract] ( 244 ) HTML (1 KB)  PDF (2109 KB)  ( 71 ) 1592 Impacts of miR-208b-3p on the Proliferation Invasion and Epithelial Mesenchymal Transition of Non-Small Cell Lung Cancer Cells by Regulating SOX4 DING Qiuli, QIU Qi, PENG Meijun, et al DOI: 10.3969/j.issn.1006-6233.2025.10.02 Objective: To explore the impacts of microRNA-208b-3p (miR-208b-3p) on the proliferation, invasion, and epithelial mesenchymal transition of non-small cell lung cancer cells by regulating sex determining region Y-box 4 (SOX4). Methods: Cancer tissues and adjacent tissues were collected from non-small cell lung cancer patients, and human non-small cell lung cancer cells A549, H1299, H460 and human normal lung epithelial cells 16HBE were cultured. The qRT-PCR method was used to measure miR-208b-3p and SOX4 mRNA in the aforementioned tissues and cells. H1299 cells were grouped into control group, miR-NC group, miR-208b-3p group, miR-208b-3p+pcDNA group, and miR-208b-3p+SOX4 group. CCK-8 method and clone formation assay were used to measure cell proliferation. Transwell experiment was used to measure cell invasion. Flow cytometry was used to measure cell apoptosis. In addition, western blot was used to measure the SOX4, Bax, Bcl-2, E-cadherin, and Vimentin proteins in cells. Results: Compared with adjacent tissues, miR-208b-3p was decreased and SOX4 mRNA was increased in lung cancer tissues (P<0.05). Compared with 16HBE cells, miR-208b-3p decreased in A549, H1299, and H460 cells, while SOX4 mRNA increased (P<0.05). Compared with the miR-NC group, the miR-208b-3p group showed increases in miR-208b-3p, apoptosis rate, Bax and E-cadherin proteins in H1299 cells, and decreases in SOX4 mRNA and protein, OD value, clone formation rate, Bcl-2 and Vimentin proteins, and number of invasive cells (P<0.05). Compared with the miR-208b-3p+pcDNA group, the miR-208b-3p+SOX4 group showed decreased apoptosis rate, Bax and E-cadherin proteins in H1299 cells, and an increase in SOX4 mRNA and protein, OD value, clone formation rate, Bcl-2 and Vimentin proteins, and number of invasive cells (P<0.05). Conclusion: MiR-208b-3p may inhibit the proliferation, invasion, and epithelial mesenchymal transition of non-small cell lung cancer cells by targeting the inhibition of SOX4. 2025 Vol. 31 (10): 1592-1600 [Abstract] ( 137 ) HTML (1 KB)  PDF (1740 KB)  ( 22 ) 1600 miR-371a-5p Targeted Regulation of LZTS2 Promotes Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells ZHANG Jie, et al DOI: 10.3969/j.issn.1006-6233.2025.10.03 Objective: To investigate the association and functional role of miR-371a-5p and leucine zipper tumor suppressor 2 (LZTS2) in triple-negative breast cancer (TNBC). Methods: TNBC tissues and adjacent non-tumor tissues were collected from 60 patients who underwent radical surgery at our hospital between October 2021 and October 2023. The relative expression levels of LZTS2 mRNA in TNBC tissues and cells were analyzed by qRT-PCR. CCK-8, scratch healing assay and Transwell were performed to evaluate the cell proliferation, migration and invasion. Expression of LZTS2 and epithelial-mesenchymal transition (EMT)-related proteins were detected by Western blot. The relationship between miR-371a-5p and LZTS2 in TNBC cells was detected by Dual-luciferase reporter assays. Xenograft tumor experiments were performed to examine the impacts of miR-371a-5p on LZTS2 expression and tumor growth in vivo. Results: LZTS2 mRNA expression was decreased in TNBC tissues and cells (t=98.590,F=178.41,P<0.05); overexpression of LZTS2 can inhibit invasion, migration, proliferation, and EMT of TNBC cells (P<0.05); LZTS2 was a target gene of miR-371a-5p (t=26.778, P<0.05); In addition, overexpression of LZTS2 weakened the promotion effect of overexpression of miR-371a-5p on TNBC cells proliferation, migration, invasion and EMT (P<0.05). In vivo experiments confirmed that miR-371a-5p regulated LZTS2 mRNA to inhibit tumor growth (P<0.05). Conclusion: miR-371a-5p may accelerate TNBC cell proliferation, migration, invasion, and EMT by targeting LZTS2, providing a new potential therapeutic target for TNBC. 2025 Vol. 31 (10): 1600-1606 [Abstract] ( 135 ) HTML (1 KB)  PDF (2481 KB)  ( 13 ) 1607 Effect of Arctigenin on Hypoxia/Reoxygenation-Induced Apoptosis of HK-2 Cells by Regulating the SDF-1/CXCR4 Signaling Pathway LI Lanmei, BAI Weiwei, CHEN Na, et al DOI: 10.3969/j.issn.1006-6233.2025.10.04 Objective: To investigate the effect of arctigenin (ARC) on hypoxia/reoxygenation (H/R) - induced apoptosis of human renal proximal tubular epithelial HK-2 cells by regulating the stromal cell-derived factor-1 (SDF-1)/CXCR4 signaling pathway. Methods: HK-2 cells were cultured in vitro and separated into Control group, Model group, L-ARC, M-ARC, H-ARC group (5, 10, 20 μmol/L ARC), and AMD group (20 μmol/L ARC+5 μmol/L SDF-1/CXCR4 signaling pathway inhibitor AMD3100) randomly. Except for the normal culture of the Control group, H/R cell models were established in all other groups. MTT and clone formation experiments were applied to detect the proliferation of cells in each group. DCFH-DA fluorescent probe was applied to detect the level of reactive oxygen species (ROS). The reagent kit was used to detect changes in the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) of cells in each group. Flow cytometry was applied to detect apoptosis of cells in various groups. Western blotting (WB) was applied to detect the expression of apoptosis related proteins (Bcl-2, Bax) and SDF-1/CXCR4 signaling pathway related proteins (SDF-1, CXCR4) of cells in each group. Results: Compared with the Control group, the proliferation rate, clone number, SOD, and Bcl-2 protein expression of HK-2 cells in the Model group decreased, while the apoptosis rate, ROS, MDA, Bax, SDF-1, and CXCR4 protein expression increased (P<0.05). Compared with the Model group, the proliferation rate, clone number, SOD, Bcl-2, SDF-1, and CXCR4 protein expression of cells in the L-ARC group, M-ARC group, and H-ARC group increased, while the apoptosis rate, ROS, MDA, and Bax protein expression decreased (P<0.05). Compared with the H-ARC group, the proliferation rate, clone number, SOD, Bcl-2, SDF-1, and CXCR4 protein expression in AMD group reduced, while the apoptosis rate, ROS, MDA, and Bax protein expression increased (P<0.05). Conclusion: ARC may alleviate oxidative stress response, inhibit H/R-induced HK-2 cell apoptosis, promote cell proliferation, and improve renal proximal tubular epithelial cell damage by promoting the activation of the SDF-1/CXCR4 signaling pathway. 2025 Vol. 31 (10): 1607-1612 [Abstract] ( 133 ) HTML (1 KB)  PDF (1955 KB)  ( 25 ) 1613 Study on the Mechanism of Oxidative Damage of Cartilage Tissue in Knee Osteoarthritis Rats by Electroacupuncture through the SIRT1/NRF2/HO-1 Pathway ZHANG Yin, et al DOI: 10.3969/j.issn.1006-6233.2025.10.05 Objective: To investigate the effects of electroacupuncture on oxidative damage of cartilage tissue and SIRT1/NRF2/HO-1 pathway in knee osteoarthritis (KOA) rats. Methods: A rat model of knee osteoarthritis was constructed, and successfully modeled rats were stochastically grouped into model group (KOA group), electroacupuncture group (EA group), and electroacupuncture+pathway inhibitor group (EA+EX527 group), each with 12. Additionally, 12 healthy normal rats were made the blank control group (Control group). Rats in each group were subjected to Lequesne scoring. ELISA was used to detect inflammatory markers in joint fluid and oxidative stress markers in cartilage tissue. DCFH-DA method was used to test ROS in cartilage tissue. HE staining was performed to observe the pathological morphology of cartilage. TUNEL staining was performed to test chondrocyte apoptosis. Immunohistochemistry was used to test cartilage injury related proteins. Western blot was performed to detect SIRT1/NRF2/HO-1 pathway related proteins. Results: The knee joint cartilage injury in the KOA group was more severe than that in the Control group, with abnormal morphological structure, damaged cartilage membrane, blurred adhesive lines, sparse and disordered arrangement of chondrocytes, the Lequesne score, IL-6, TNF-α, ROS fluorescence intensity, MDA, apoptosis rate, C-caspase-9, C-caspase-3, MMP3, and MMP13 raised, while the SOD and GSH-Px activities, SIRT1, Keap1, NRF2, and HO-1 reduced (P<0.05). The knee cartilage injury in the EA group improved compared to the KOA group, with a relatively normal morphology and structure, the cartilage surface was relatively smooth, and the chondrocytes were arranged neatly, the Lequesne score, IL-6, TNF-α, ROS fluorescence intensity, MDA, apoptosis rate, C-caspase-9, C-caspase-3, MMP3, and MMP13 reduced, while the SOD and GSH-Px activities, SIRT1, Keap1, NRF2, and HO-1 raised (P<0.05). The knee cartilage injury in the EA+EX527 group was more severe than that in the EA group, the Lequesne score, IL-6, TNF-α, ROS fluorescence intensity, MDA, apoptosis rate, C-caspase-9, C-caspase-3, MMP3, and MMP13 raised, while the SOD and GSH-Px activities, SIRT1, Keap1, NRF2, and HO-1 reduced (P<0.05). Conclusion: Electroacupuncture can improve oxidative damage in cartilage tissue of KOA rats, which is related to the activation of SIRT-1-Nrf2-HO-1 signaling pathway. 2025 Vol. 31 (10): 1613-1619 [Abstract] ( 123 ) HTML (1 KB)  PDF (2448 KB)  ( 17 ) 1619 Impacts of Remimazolam on Postoperative Cognitive Function and Neural Synapses in Elderly Rats Through PERK/ATF4/CHOP Pathway ZHANG Yanpeng, LU Jieping DOI: 10.3969/j.issn.1006-6233.2025.10.06 Objective: To explore the impacts of remimazolam on postoperative cognitive function and neural synapses in elderly rats by regulating protein kinase R-like endoplasmic reticulum kinase (PERK)/activating transcription factor 4 (ATF4)/transcription factor C/EBP-homologous protein (CHOP) pathway. Methods: A cognitive impairment rat model was established by partial liver resection and grouped into a model group, a remimazolam group (6mg/kg), a PERK activator group (5mL/kg MK-28), and a remimazolam+PERK activator group (6mg/kg remimazolam and 5mL/kg MK-28). Rats without liver resection were selected as the control group. The new object recognition experiment and water maze experiment were used to evaluate the cognitive function of each group. Transmission electron microscopy was used to observe the changes in synaptic structure of hippocampal tissue in each group. TUNEL staining was used to observe the apoptosis of hippocampal neurons of rats. Western blot was used to measure the postsynaptic density-95 (PSD95), synaptophysin (SYN), PERK, phosphorylated (p) - ERK, ATF4, and CHOP proteins in the hippocampal tissues of rats. Results: For the control group, the model group had unclear synaptic gaps, severe synaptic damage, decreased platform crossing frequency, total swimming distance, new object recognition index, synaptic number, PSD95 and SYN proteins, but increased escape latency, neuronal apoptosis rate, p-PERK, ATF4, and CHOP proteins (P<0.05). For the model group, the remimazolam group showed obvious improvement in synaptic damage in rats, raised synaptic number, platform crossing frequency, total swimming distance, new object recognition index, PSD95 and SYN proteins, but decreased neuronal apoptosis rate, escape latency, p-PERK, ATF4, and CHOP proteins (P<0.05), however, the PERK activator group showed aggravated synaptic damage, decreased platform crossing frequency, total swimming distance, new object recognition index, synaptic number, PSD95 and SYN proteins, but increased escape latency, neuronal apoptosis rate, p-PERK, ATF4, and CHOP proteins (P<0.05). Intervention with PERK activator on the basis of treatment with remimazolam could reverse the improvement effect of remimazolam on cognitive function in model rats (P<0.05). Conclusion: Remimazolam may improve postoperative cognitive function and neural synapse in elderly rats by inhibiting PERK/ATF4/CHOP pathway. 2025 Vol. 31 (10): 1619-1625 [Abstract] ( 124 ) HTML (1 KB)  PDF (1594 KB)  ( 210 ) 1625 Mechanism of Downregulating ALKBH5 in Improving Pulmonary Fibrosis in Rats by Regulating Macrophage Polarization by TGF-β1/Smad Pathway DAI Deyu, DANG Hui, WANG Xue, et al DOI: 10.3969/j.issn.1006-6233.2025.10.07 Objective: To explore the role of downregulating AlkB homolog 5 (ALKBH5) in rats with pulmonary fibrosis (PF) and its potential mechanism based on the transforming growth factor-β1 (TGF-β1)/Smad signaling pathway. Methods: A rat model of PF was established by intratracheal instillation of bleomycin. Successfully modeled Sprague-Dawley (SD) rats were divided into the model group (PF + no drug treatment), si-NC group (PF + transfection with si-NC via tail vein injection), si-ALKBH5 group (PF + transfection with si-ALKBH5 via tail vein injection), and pathway activator group (PF + transfection with si-ALKBH5 + 30 mg/kg TGF-β1 pathway activator SRI-011381 via tail vein injection). Another 12 normal rats were selected as the control group. RT-qPCR was used to detect the mRNA levels of ALKBH5, inducible nitric oxide synthase (iNOS), cluster of differentiation 86 (CD86), arginase-1 (Arg-1), and mannose receptor (CD206) in lung tissues. A pulmonary function detector was used to measure tissue damping (G), respiratory system resistance (Rrs), respiratory system compliance (Crs), and tissue elastance (H) in rats of each group. Hematoxylin-Eosin (HE) staining and Masson staining were performed for histopathological analysis of the lungs. Western Blot was used to detect the expression levels of ALKBH5, type I collagen (Col I), fibronectin (FN), α-smooth muscle actin (α-SMA), and proteins related to the TGF-β1/Smad pathway. Results: Compared with the control group, the lung structure of rats in the model group was damaged, with thickened alveolar septa and necrosis of alveolar epithelial cells; the levels of G, Crs, Arg-1 mRNA, and CD206 mRNA were significantly decreased (P<0.05); while the levels of ALKBH5 mRNA and protein, Rrs, H, collagen fiber deposition, Col I, FN, α-SMA, iNOS mRNA, CD86 mRNA, TGF-β1, p-Smad2, and p-Smad3 were significantly increased (P<0.05). Compared with the model group and si-NC group, the alveolar septa in the si-ALKBH5 group and pathway activator group were slightly thickened, and the lung tissue structure was relatively intact; the levels of G, Crs, Arg-1 mRNA, and CD206 mRNA were significantly increased (P<0.05); while the levels of ALKBH5 mRNA and protein, Rrs, H, collagen fiber deposition, Col I, FN, α-SMA, iNOS mRNA, CD86 mRNA, TGF-β1, p-Smad2, and p-Smad3 were significantly decreased (P<0.05). Compared with the pathway activator group, the degree of lung tissue injury in the si-ALKBH5 group was improved; the levels of G, Crs, Arg-1 mRNA, and CD206 mRNA were significantly increased (P<0.05); while the levels of ALKBH5 mRNA and protein, Rrs, H, collagen fiber deposition, Col I, FN, α-SMA, iNOS mRNA, CD86 mRNA, TGF-β1, p-Smad2, and p-Smad3 were significantly decreased (P<0.05). Conclusion: Downregulating ALKBH5 can alleviate pathological damage and collagen deposition in lung tissue, reduce lung function impairment, inhibit PF in rats, and suppress the polarization of macrophages to the M2 type. The mechanism may be related to the inhibition of the TGF-β1/Smad signaling pathway. 2025 Vol. 31 (10): 1625-1632 [Abstract] ( 123 ) HTML (1 KB)  PDF (2217 KB)  ( 81 ) 1632 The Effect of miR-30a-5p on Vascular Calcification in Rats with Chronic Kidney Disease by Targeting Runx2 YU Pei, et al DOI: 10.3969/j.issn.1006-6233.2025.10.08 Objective: To investigate the effect of microRNA-30a-5p (miR-30a-5p) on vascular calcification in chronic kidney disease (CKD) rats by targeting Runt-related transcription factor-2 (Runx2). Methods: CKD model rats were constructed and randomly grouped into model group, miR-30a-5p negative control group (miR-30a-5p-NC group), miR-30a-5p mimic group (miR-30a-5p mimics group), miR-30a-5p mimic+empty plasmid group (miR-30a-5p+pcDNA-NC group), and miR-30a-5p mimic+Runx2 overexpression group (miR-30a-5p+Runx2 group). Healthy rats were made the control group. RT-qPCR was used to detect the miR-30a-5p and Runx2 mRNA in the aorta. The 24-hour urine protein content (reagent kit method), serum creatinine (SCr), calcium (Ca), phosphorus (P), blood urea nitrogen (BUN), and serum cystatin C (CysC) in rats (biochemical analyzer); the pathological changes in renal tissue (HE staining method); aortic calcified nodules (Alizarin Red staining); calcium content and ALP activity of vascular wall (kit method); the positive expression of α-SMA and OPN in the aorta (immunohistochemistry), and the protein contents of Runx2, BMP2, and OCN (Western blot) were measured. Dual luciferase was used to determine the targeting relationship between miR-30a-5p and Runx2. Results: The kidney tissue of rats in the model group showed conspicuous pathological changes and aggravated renal aortic calcification, and the 24-hour urinary protein, renal function indexes, Ca content, ALP activity, OPN positive expression, and Runx2, BMP2, OCN protein contents in the aorta were conspicuously increased, while the miR-30a-5p and α-SMA positive expression in the aorta were conspicuously decreased (P<0.05). Compared with the miR-30a-5p-NC group, the miR-30a-5p mimics group showed reduced renal tissue damage and renal aortic calcification in rats, and the 24-hour urinary protein, renal function indexes, Ca content, ALP activity, OPN positive expression, and Runx2, BMP2, OCN protein contents in the aorta were conspicuously decreased, while the miR-30a-5p and α-SMA positive expression in the aorta were conspicuously increased (P<0.05). Compared with the miR-30a-5p+pcDNA-NC group, the miR-30a-5p+Runx2 group showed severe renal tissue damage, aggravated renal aortic calcification, decreased renal function, and the α-SMA positive expression in the aorta was conspicuously reduced, while the OPN positive expression and Runx2, BMP2, and OCN protein contents were conspicuously increased (P<0.05). The dual luciferase reporter gene experiment demonstrated a targeted relationship between miR-30a-5p and Runx2. Conclusion: MiR-30a-5p may enhance renal function, improve kidney injury, and inhibit vascular calcification in CKD rats by targeting Runx2. 2025 Vol. 31 (10): 1632-1639 [Abstract] ( 116 ) HTML (1 KB)  PDF (1972 KB)  ( 7 ) 1639 Effects of CD200 and Its Receptor CD200R Pathway on Airway Injury and Th1/Th2 Imbalance in Asthmatic Rats NIGELA Yilihamu, LI Jingjing, NUERAMINA Tieliwaerdi, et al DOI: 10.3969/j.issn.1006-6233.2025.10.09 Objective: To explore the effects of differentiation cluster 200 (CD200) and its receptor CD200R pathway on asthmatic model rats and its mechanism. Methods: A total of 40 male SD rats were randomly divided into control group (treated with normal saline without modeling), model group (treated with normal saline before building asthma model), IgG1Fc group (treated with IgG1Fc before building asthma model), and CD200Fc group (treated with CD200Fc before building asthma model), with 10 rats in each group. The asthma model was established by intrabitoneal ovalbumin sensitization and atomization inhalation stimulation. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expression changes of CD200 and CD200R in rat lung tissue after modeling. Hematoxylin-eosin (HE) staining was used to observe the lung histopathological changes in each group, and bronchial wall thickness (Wat) and smooth muscle thickness (Wam) were measured, Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of T helper 1 (Th1) related factors interleukin-12 (IL-12), interferon-γ (IFN-γ) and T helper 2 (Th2) related factors interleukin-4 (IL-4), interleukin-13 (IL-13) in bronchoalveolar lavage fluid of rats in each group, Western blot was used to detect the protein expression of T-box transcription factor (T-bet) and GATA binding protein 3 (GATA-3) in lung tissue of each group, Flow cytometry was used to detect the distribution of Th1 and Th2 subpopulations in spleen and to calculate the Th1/Th2 ratio. Results: Compared with the control group, the mRNA and protein relative expressions of CD200 and CD200R in lung tissues of model group were significantly down-regulated (P<0.05). Compared with the control group, the alveolar wall of the model group was thickened, part of the alveolar collapsed, and a large number of inflammatory cells gathered around the airway, Wat and Wam were significantly increased (P<0.05), the levels of IL-12 and IFN-γ in bronchoalveolar lavage fluid were significantly decreased, and the levels of IL-4 and IL-13 were significantly increased (P<0.05), the T-bet protein expression was significantly down-regulated and the GATA-3 protein expression was significantly up-regulated in lung tissue (P<0.05), CD4+IFN-γ+ labeled Th1 and Th1/Th2 were significantly decreased in spleen, and CD4+IL-4+ labeled Th2 was significantly increased (P<0.05). Compared with model group, the symptoms of alveolar lesions, thickening of airway wall and smooth muscle, and aggregation of inflammatory cells in CD200Fc group were significantly reduced, Wat and Wam were significantly decreased (P<0.05), the levels of IL-12 and IFN-γ in bronchoalveolar lavage fluid were significantly increased, the levels of IL-4 and IL-13 were significantly decreased (P<0.05), the T-bet protein expression was significantly up-regulated and the GATA-3 protein expression was significantly down-regulated in lung tissue (P<0.05), while CD4+IFN-γ+ labeled Th1 and Th1/Th2 were significantly increased and CD4+IL-4+ labeled Th2 were significantly decreased in spleen (P<0.05). Conclusion: The expression of CD200 and its receptor CD200R is down-regulated in lung tissue of asthmatic rats. Preconditioning of CD200Fc can alleviate airway injury and improve Th1/Th2 imbalance in asthmatic rats, thus playing a therapeutic role. 2025 Vol. 31 (10): 1639-1645 [Abstract] ( 96 ) HTML (1 KB)  PDF (1862 KB)  ( 11 ) 1646 Molecular Mechanisms of MEG3 in Suppressing Malignant Progression of Nasopharyngeal Carcinoma through a Multidimensional Regulatory Network BAO Weijing, NING Jiayu, WEI Rifu, et al DOI: 10.3969/j.issn.1006-6233.2025.10.010 Objective: To illustrate the regulatory role of long non-coding RNA MEG3 on malignant biological behaviors (proliferation, migration, invasion, and apoptosis) in nasopharyngeal carcinoma (NPC) cells and to analyze its molecular mechanisms. Methods: MEG3 overexpression models were constructed in NPC cell lines TW03 and CNE-1. qRT-PCR was employed to detect gene expression levels, while CCK-8 assay, Transwell experiments, and flow cytometry were utilized to systematically evaluate the effects of MEG3 on cell proliferation, migration, invasion, and apoptosis. Results: Compared with normal nasopharyngeal epithelial cells NP69, MEG3 expression was significantly downregulated in NPC cells (0.45±0.03 vs 1.00±0.00, P<0.05). Overexpression of MEG3 elevated its mRNA level to 5.29±0.05 (F=398.057, P<0.01), and markedly suppressed NPC cell proliferation (96h OD value: 0.72±0.04 vs 1.25±0.05, F=9.543, P<0.05), migration (32.6±3.1 vs 156.3±8.2, F=1336.342, P<0.05), and invasion (28.4±2.9 vs 142.5±7.5, F=1388.434, P<0.05), while inducing an increased apoptosis rate of 8.09±0.11% (F=801.043, P<0.05). Conclusion: MEG3 exhibits a loss-of-expression signature in NPC, and its functional restoration effectively restrains malignant phenotypes of tumor cells by targeting the inhibition of epithelial-mesenchymal transition (EMT), suggesting MEG3 as a potential therapeutic target for molecularly targeted therapy in NPC. 2025 Vol. 31 (10): 1646-1650 [Abstract] ( 109 ) HTML (1 KB)  PDF (1124 KB)  ( 7 ) 1650 Effect of miR-32-5p on Malignant Biological Behavior of Glioma Cells by Targeting Regulation of E2F7 SUN Xingwang, LIANG Yun, WANG Guanpeng, et al DOI: 10.3969/j.issn.1006-6233.2025.10.011 Objective: To explore the effect of microRNA-32-5p (miR-32-5p) on the malignant biological behavior of glioma cells by targeting the regulation of E2F transcription factor 7 (E2F7). Methods: This study analyzed 45 paired glioma and adjacent tissues from surgically treated patients (from March 2023 to August 2024) using qRT-PCR to measure miR-32-5p and E2F7 mRNA expression. In vitro, U87MG cells were divided into seven groups: NC, miR-NC, miR-32-5p mimic, sh-NC, sh-E2F7, miR-32-5p mimic+pcDNA-NC, and miR-32-5p mimic+pcDNA-E2F7. Functional assays included clonogenic, flow cytometry (apoptosis), wound healing, and Transwell (migration/invasion) tests. qRT-PCR was used to assess miR-32-5p/E2F7 expression, while Western blot was used to analyze E2F7, Cyclin D1, PCNA, Caspase-3, Bax, MMP-2, and TIMP-1 protein levels. The miR-32-5p/E2F7 targeting relationship was validated by dual-luciferase reporter assay. Results: Compared with adjacent tissues, the miR-32-5p in cancer tissues was clearly reduced, while the E2F7 mRNA was clearly increased (P<0.05). For the NC group, there were no clear changes in various indicators in the miR-NC group and sh-NC group (P>0.05). For the miR-NC group or sh-NC group, the miR-32-5p mimic group or sh-E2F7 group showed a decrease in the proliferation, migration, and invasion abilities of U87MG cells. The E2F7 mRNA, E2F7, Cyclin D1, PCNA, and MMP-2 proteins were clearly reduced, while the apoptosis rate, miR-32-5p, and the Caspase-3, Bax, and TIMP-1 proteins were clearly increased (P<0.05). For the miR-32-5p mimic+pcDNA-NC group, the miR-32-5p mimic+pcDNA-E2F7 group showed increased proliferation, migration, and invasion abilities of U87MG cells. The E2F7 mRNA, E2F7, Cyclin D1, PCNA, and MMP-2 proteins were increased, while the apoptosis rate, miR-32-5p, and the Caspase-3, Bax, and TIMP-1 proteins were reduced (P<0.05). The dual luciferase assay demonstrated a targeted relationship between miR-32-5p and E2F7 (P<0.05). Conclusion: MiR-32-5p can inhibit the malignant biological behavior of glioma cells by targeting and downregulating E2F7. 2025 Vol. 31 (10): 1650-1657 [Abstract] ( 115 ) HTML (1 KB)  PDF (2646 KB)  ( 5 ) 1657 The Mechanism of Lutein of Zephyr on Secretory Otitis Media Rats Based on AMPK/NLRP3 Pathway DENG Xiangyu, CHEN Hongbing DOI: 10.3969/j.issn.1006-6233.2025.10.012 Objective: To explore the impact of eupafolin on rats with secretory otitis media via the AMPK/NLRP3 pathway and preliminarily probe its potential mechanism. Methods: Fifty 6-8-week-old rats were randomly allocated into 5 groups: control, model, low-dose Eupolyphaga lutein (20mg/kg), high-dose Eupolyphaga lutein (80mg/kg), and high-dose Eupolyphaga lutein+AMPK inhibitor (80mg/kg + 20mg/kg), with 10 rats in each. Secretory otitis media rat models were established by endotoxin injection. Post-modeling, the low-, high-dose eupafolin and high-dose eupafolin+AMPK inhibitor groups received corresponding intraperitoneal drug injections, while the control and model groups got equal-volume saline. HE staining was used to observe middle ear mucosal tissue morphology; a micrometer, to measure its thickness; an ABR instrument, to detect the ABR response threshold; enzyme-linked immunosorbent assay, to test serum inflammatory factor levels; fluorescence quantitative PCR, to detect IFN-γ and IL-4 gene expression; and Western blot, to examine AMPK/NLRP3 pathway-related protein expression in rat mucosal tissue. Results: Compared to the control group, the model group had aggravated middle ear mucosal tissue pathology, increased thickness, ABR response threshold, inflammatory factor levels and NLRP3 protein expression (P<0.05), but decreased IFN-γ/IL-4 ratio and p-AMPK protein expression (P<0.05). Compared with the model group, the low- and high-dose eupafolin groups showed alleviated middle ear mucosal tissue pathology, reduced thickness, ABR response threshold, inflammatory factor levels and NLRP3 protein expression (P<0.05), while the IFN-γ/IL-4 ratio and p-AMPK protein expression were increased (P<0.05). Compared with the high-dose eupafolin group, the high-dose+AMPK inhibitor group had reversed the above indicators (P<0.05). Conclusion: Eupolyphaga lutein can markedly ameliorate the inflammatory response in rats with secretory otitis media. Its possible mechanism is activating the AMPK pathway, suppressing NLRP3 inflammasome activation, reducing inflammatory factor release, and thereby mitigating middle ear mucosal tissue damage. 2025 Vol. 31 (10): 1657-1664 [Abstract] ( 129 ) HTML (1 KB)  PDF (1349 KB)  ( 14 ) 1664 Relationship between Serum Visfatin HMGB1 and Ghrelin Levels and the Severity and Prognosis of Patients with Acute Pancreatitis YIN Ruozhe, ZHOU Yang, PAN Longfei DOI: 10.3969/j.issn.1006-6233.2025.10.013 Objective: To investigate the relationship between serum levels of Visfatin, high mobility group protein B1 (HMGB1) and Ghrelin and disease severity and prognosis in patients with acute pancreatitis (AP). Methods: Totally 198 AP patients in the hospital were selected from July 2019 to July 2023. According to the revised Atlanta classification (RAC), mild patients were included in mild group (n=113), and moderately severe and severe patients were enrolled as moderate-to-severe group (n=85). The enrolled patients were classified into survival group (n=182) and death group (n=16) by means of 28-day survival status. The differences in clinical data and serum levels of Visfatin, HMGB1 and Ghrelin were compared among different groups. Correlation analysis was used to assess the relationship between serum Visfatin, HMGB1 and Ghrelin levels and modified CT severity index (MCTSI), acute physiological and chronic health evaluation score (APACHE II) and determinant-based classification (DBC) in AP patients. Logistic regression analysis was used to analyze the factors leading to poor prognosis in patients with acute pancreatitis. Receiver operating characteristic curve (ROC) was used to evaluate the value of serum Visfatin, HMGB1 and Ghrelin on predicting poor prognosis in patients with acute pancreatitis. Results: MCTSI score, APACHE II score and levels of serum Visfatin, HMGB1 and Ghrelin in mild group were lower than those in moderate-to-severe group (P<0.05). MCTSI score, APACHE II score, serum Visfatin, HMGB1 and Ghrelin in survival group were lower while the proportion of DBC mild disease was higher than that in death group (P<0.05). Serum Visfatin, HMGB1 and Ghrelin in AP patients were proportional to MCTSI score, APACHE II score and DBC grading (P<0.05). High serum Visfatin, HMGB1 and Ghrelin were independent risk factors for poor prognosis in AP patients (P<0.05). The combined detection of serum Visfatin, HMGB1 and Ghrelin predicted the prognosis of AP patients with an AUC of 0.892, which was better than that of serum Visfatin alone (P<0.05). Conclusion: Serum Visfatin, HMGB1 and Ghrelin are closely related to the disease severity and prognosis in AP patients. The higher serum Visfatin, HMGB1 and Ghrelin, the more severe the disease and the higher the risk of 28-day death, and the combination of the three indicators can better evaluate the prognosis in AP patients. 2025 Vol. 31 (10): 1664-1670 [Abstract] ( 123 ) HTML (1 KB)  PDF (1294 KB)  ( 17 ) 1670 Expression and Clinical Significance of CBX2 and CBX7 in Mucoepidermoid Carcinoma of Salivary Gland ZHANG Xuan, YANG Yongchao, LI Chao, et al DOI: 10.3969/j.issn.1006-6233.2025.10.014 Objective: To explore the expression and clinical significance of CBX2 and CBX7 in mucoepidermoid carcinoma (MEC) of the salivary gland. Methods: 94 patients with salivary gland MEC in our hospital from January 2017 to December 2021 were designated as subjects. Paraffin specimens of cancer tissue and normal salivary gland tissue adjacent to cancer were collected. Immunohistochemistry was used to detect CBX2 and CBX7 proteins in salivary gland MEC cancer tissues and adjacent tissues. Clinical pathological data and prognosis of patients were collected. Kaplan-Meier method was used to analyze the relationship between CBX2 and CBX7 in cancer tissues and the prognosis of salivary gland MEC. Cox proportional hazards regression model was used to analyze the factors influencing the prognosis of salivary gland MEC. Results: The positive rates of CBX2 and CBX7 in MEC cancer tissues of salivary glands were higher than those in adjacent tissues (60.64% vs 20.21%, 56.38% vs 25.53%, P<0.05). The CBX2 and CBX7 in cancer tissues of salivary gland MEC patients with different onset sites, ages, and genders showed no statistically great difference (P>0.05). For patients with tumor diameter<2 cm, pathological grade I+II, non lymph node metastasis, and TNM stage I+II, the patients with tumor diameter ≥ 2 cm, pathological grade III, lymph node metastasis, and TNM stage III+IV had higher positive rates of CBX2 and CBX7 in cancer tissue (P<0.05). The positive CBX2 and CBX7 were risk factors for mortality within 3 years in patients with salivary gland MEC (P<0.05). Patients with positive CBX2 had a lower 3-year survival rate (38/57, 66.67%) than those with negative CBX2 (32/37, 86.49%) (χ2=4.635, P<0.05). Patients with positive CBX7 had a lower 3-year survival rate (35/53, 66.04%) than those with negative CBX7 (35/41, 85.37%) (χ2=4.496, P<0.05). Conclusion: The positive rates of CBX2 and CBX7 in cancer tissues of salivary gland MEC patients are elevated, and their expression is related to the clinical pathology and prognosis. 2025 Vol. 31 (10): 1670-1675 [Abstract] ( 123 ) HTML (1 KB)  PDF (1861 KB)  ( 12 ) 1675 The Correlation among Heart Rate Variability Blood Pressure and Urine Protein-to-Creatinine Ratio in Gestational Hypertension Complicated with Diabetes WANG Xiaoning, WEI Xueyang, DU Xiaojie, et al DOI: 10.3969/j.issn.1006-6233.2025.10.015 Objective: To explore the correlation between heart rate variability,blood pressure levels,and urine protein/creatinine ratio in patients with gestational hypertension and diabetes mellitus. Methods: A total of 106 patients with gestational hypertension complicated by diabetes admitted to our hospital from June 2022 to June 2024 were selected as the study group.Additionally,106 patients with gestational hypertension admitted to our hospital during the same period were selected as the HDP group,and 106 normal pregnant women admitted to our hospital during the same period were selected as the control group.The differences in blood pressure levels,heart rate variability,and urine protein-to-creatinine ratio among the three groups were compared,and Pearson's correlation analysis was performed to assess their associations.ROC analysis was conducted to evaluate the diagnostic value. Results: The 24h DBP,24h SBP,daytime DBP and SBP,and nighttime DBP and SBP in the study group were higher than those in the HDP group and control group,with statistically significant differences (P<0.05).The SDANN,PNN50,RMSSD,and SDNN in the study group were lower than those in the HDP group and control group,while ACR was higher in the study group than in the HDP group and control group; the differences were statistically significant (P<0.05).Pearson's correlation analysis showed a positive correlation between ACR and 24hDBP,24hSBP (r=0.755,0.724,P<0.05); a negative correlation between 24hDBP and PNN50 (r=-0.259,P<0.05); and a negative correlation between 24hSBP and SDANN,PNN50,RMSSD,and SDNN (r=-0.213,-0.355,-0.194,-0.256,P<0.05).ROC analysis showed that the AUCs for diagnosing gestational hypertension and diabetes mellitus using urine protein/creatinine ratio,blood pressure levels,and heart rate variability were 0.885,0.991,and 0.931,respectively; the sensitivities were 89.6%,99.1%,and 90.6%,respectively; and the specificities were 70.8%,90.6%,and 88.7%,respectively. Conclusion: Patients with gestational hypertension and diabetes mellitus exhibit decreased heart rate variability,which is negatively correlated with increased blood pressure,while ACR is positively correlated with increased blood pressure.This study emphasizes the importance of heart rate variability,blood pressure levels,and ACR in assessing the condition and predicting complications,providing a reference for clinical practice. 2025 Vol. 31 (10): 1675-1680 [Abstract] ( 125 ) HTML (1 KB)  PDF (1229 KB)  ( 13 ) 1680 Expression of Serum sTWEAK and Omentin Levels in Hypertensive Pregnancy and Their Clinical Value HUA Jing, HU Qun, XIE Tao, et al DOI: 10.3969/j.issn.1006-6233.2025.10.016 Objective: To explore the serum levels of soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) and Omentin in patients with hypertensive disorders complicating pregnancy (HDCP) and their diagnostic value. Methods: A total of 88 patients with HDCP who were admitted to our hospital from January 2020 to January 2024 were gathered as the observation group. According to the severity of the patient's condition, they were separated into group A (hypertension), group B (mild preeclampsia), and group C (severe preeclampsia), with 49 cases, 23 cases, and 16 cases, respectively; 88 healthy pregnant women who underwent prenatal examinations in our hospital were made the control group. Enzyme linked immunosorbent assay (ELISA) was applied to detect serum levels of sTWEAK and Omentin. Relationship between serum sTWEAK, Omentin levels and blood pressure in HDCP patients were analyzed by Pearson correlation analysis. Logistic regression was applied to analyze the influencing factors of HDCP. Receiver operating characteristic (ROC) curve was applied to analyze the clinical diagnostic value of serum sTWEAK and Omentin levels for preeclampsia. Results: The PLT, MPV, PDW, PagT, systolic blood pressure, and diastolic blood pressure in the observation group were greatly higher than those in the control group (P<0.05), while the serum levels of sTWEAK and Omentin were greatly lower than those in the control group (P<0.05). The serum levels of sTWEAK and Omentin in group A, group B, and group C decreased sequentially (P<0.05). Pearson correlation analysis showed that sTWEAK levels were negatively correlated with systolic and diastolic blood pressure (r=-0.497, -0.561, P<0.001) and Omentin levels were negatively correlated with systolic and diastolic blood pressure (r=-0.452, -0.529, P<0.001). STWEAK and Omentin were protective factors for patients with HDCP (P<0.05). The area under the curve (AUC) of serum sTWEAK and Omentin in diagnosing preeclampsia were 0.829 and 0.815, and the AUC of combined diagnosis was 0.892, the combination of the two was superior to the individual diagnosis of serum sTWEAK and Omentin (Z combination - STWEAK=2.136, Z combination - Omentin=2.012, all P<0.05). Conclusion: The serum levels of Omentin and sTWEAK in HDCP patients are greatly decreased, and the combination of the two has high diagnostic value for preeclampsia. 2025 Vol. 31 (10): 1680-1685 [Abstract] ( 115 ) HTML (1 KB)  PDF (1308 KB)  ( 15 ) 1686 Relationship between the Combined Detection of Serum Hcy and BTP Levels and the Pregnancy Outcomes of Patients with Severe Preeclampsia YANG Ziman, CUI Yanjie, LI Na, et al DOI: 10.3969/j.issn.1006-6233.2025.10.017 Objective: To explore the relationship between the combined detection of serum homocysteine (Hcy) and beta-trace protein (BTP) levels and the pregnancy outcomes of patients with severe preeclampsia (PE). Methods: A total of 320 PE patients from January 2021 to January 2024 were selected as the PE group, and 275 normal pregnant women during the same period were selected as the control group. According to the severity of PE, they were divided into the mild-to-moderate group (n=184) and the severe group (n=136). Then, the patients with severe PE were further divided into the favorable group (n=94) and the unfavorable group (n=42) according to the pregnancy outcomes. The serum Hcy and BTP levels were detected by ELISA from the 11th to 13th week of pregnancy. The predictive value of serum Hcy and BTP levels for the unfavorable pregnancy outcomes of patients with severe PE was analyzed by the ROC curve, and the Z-test was used to compare the differences in the area under the curve (AUC). Results: The levels of Hcy (18.06±4.38 μmoL/L) and BTP (4.44±1.15 ng/mL) in the PE group were higher than those in the control group (12.75±3.24 μmoL/L, 3.15±0.95ng/mL) (t=16.580, 14.768, all P<0.05). The levels of Hcy (21.56±5.42 μmoL/L) and BTP (5.38±1.31 ng/mL) in the severe group were higher than those in the mild-to-moderate group (15.47±4.13 μmoL/L, 3.75±0.84ng/mL) (t=13.532, 11.108, all P<0.05). The total proportion of unfavorable pregnancy outcomes in the severe group (30.88%) was higher than that in the mild-to-moderate group (17.39%) (χ2=8.006, P<0.05). The levels of Hcy (21.15±6.24 μmoL/L) and BTP (5.74±1.68 ng/mL) in the unfavorable group were higher than those in the favorable group (16.68±3.58 μmoL/L, 3.86±1.07 ng/mL) (t=5.280, 7.866, P<0.05). The AUCs of Hcy and BTP alone in predicting the unfavorable pregnancy outcomes of patients with severe PE were 0.798 and 0.803, respectively, and the AUC of the combined diagnosis of the two was 0.906, which was superior to the individual predictions (Z for the combination-Hcy=2.693, Z for the combination-BTP=2.853, P=0.007, P=0.004). Conclusion: The serum levels of Hcy and BTP increase with the aggravation of the severity of PE. Patients with severe PE have a relatively high risk of unfavorable pregnancy outcomes. The combined diagnosis of Hcy and BTP has certain value in predicting the unfavorable pregnancy outcomes of patients with severe PE, providing a data basis for clinical diagnosis and treatment. 2025 Vol. 31 (10): 1686-1691 [Abstract] ( 118 ) HTML (1 KB)  PDF (1252 KB)  ( 17 ) 1691 Predictive Value of Serum Markers in Elderly Patients with Esophageal Cancer after Thoracoscopic Surgery LI Jing, KANG Li, WANG Fang, et al DOI: 10.3969/j.issn.1006-6233.2025.10.018 Objective: To investigate the relationship between thoracoscopic surgery problems and serum marker levels in elderly esophageal cancer patients. Methods: This study recruited 108 patients with esophageal cancer who underwent thoracoscopic surgery in the Department of Thoracic Surgery of our hospital from May 2021 to May 2024. These patients were categorized into two groups: a complication group and a non-complication group. The clinical data of the patients were collected, and the levels of serum markers such as carcinoembryonic antigen (CEA), squamous cell carcinoma antigen(SCC), C-reactive protein(CRP), procalcitonin(PCT), albumin(ALB), and prealbumin(PA) were detected before surgery. Results: There were no statistically significant differences between the two groups in terms of age, gender, BMI, tumor length, tumor location, and the proportion of patients with a history of alcohol consumption, diabetes, or coronary heart disease (P>0.05). However, the proportions of patients with a history of smoking, hypertension, and chronic obstructive pulmonary disease were higher in the complication group than in the no-complication group (P<0.05). Serum CRP and SCC levels were higher in the complication group, while ALB and PA were lower compared to the no-complication group (P<0.05). Multivariate logistic regression analysis revealed that SCC, CRP, ALB, and PA are all independent influencing factors for the occurrence of complications (P<0.05). The receiver operating characteristic (ROC) curve evaluation showed that the area under the curve (AUC) for predicting complications was highest for ALB and PA, at 0.781 and 0.773 respectively. The AUC for SCC and CRP were 0.645 and 0.734 respectively. The comparisons of AUCs among these indicators were as follows: ALB vs PA (Z=0.111, P=0.911), CRP vs PA (Z=0.533, P=0.581), SCC vs PA (Z=1.855, P=0.064), ALB vs CRP (Z=0.695, P=0.487), ALB vs SCC (Z=1.912, P=0.056), CRP vs SCC (Z=1.064, P=0.287). Conclusion: It suggest that serum marker levels are of great significance in predicting complications after thoracoscopic surgery in elderly patients with esophageal cancer, and can provide a powerful reference basis for clinical early intervention and improvement of patient prognosis. 2025 Vol. 31 (10): 1691-1696 [Abstract] ( 128 ) HTML (1 KB)  PDF (1283 KB)  ( 7 ) 1696 Study on the Correlation between Serum EGR1 sVE-Cadherin Levels Disease Severity and Pregnancy Outcomes in Preeclampsia Patients LI Ruihua, MENG Haiyun, LU Sha, et al DOI: 10.3969/j.issn.1006-6233.2025.10.019 Objective: To investigate the correlation between serum early growth response factor 1 (EGR1) and soluble vascular endothelial cadherin (sVE-cadherin) levels and the severity of disease and pregnancy outcomes in preeclampsia (PE) patients. Methods: A total of 120 PE patients treated at our hospital from January 2022 to December 2024 were selected and categorized into a severe PE group (65 cases) and a mild PE group (55 cases) based on disease severity. Based on pregnancy outcomes, patients were divided into adverse outcome and favorable outcome groups. Additionally, 120 healthy pregnant women (control group) were selected in a 1:1 ratio. Serum EGR1 and sVE-cadherin levels were measured using enzyme-linked immunosorbent assay. The relationship between serum EGR1 and sVE-cadherin levels and pregnancy outcomes in PE patients was analyzed using multivariate unconditional logistic regression. The predictive value of serum EGR1 and sVE-cadherin levels for adverse pregnancy outcomes in PE patients was assessed using ROC curve analysis. Results: Compared to the control group, the PE group exhibited decreased serum EGR1 levels and increased sVE-cadherin levels (P<0.05). Compared to the mild PE group, the severe PE group showed lower serum EGR1 levels and higher sVE-cadherin levels (P<0.05). The incidence of adverse pregnancy outcomes in the 120 PE patients was 45.83% (55/120). Compared to the favorable outcome group, the adverse outcome group had decreased serum EGR1 levels and increased sVE-cadherin levels (P<0.05). Severe PE and elevated sVE-cadherin levels were identified as independent risk factors for adverse pregnancy outcomes in PE patients, while EGR1 was an independent protective factor (P<0.05). The combined prediction of serum EGR1 and sVE-cadherin levels for adverse pregnancy outcomes in PE patients had an area under the ROC curve (AUC) of 0.891, which was greater than the AUCs for EGR1 (0.796) or sVE-cadherin (0.803) alone (P<0.05). Conclusion: In PE patients, serum EGR1 levels decrease while sVE-cadherin levels increase, which are associated with disease progression and adverse pregnancy outcomes. The combined measurement of serum EGR1 and sVE-cadherin levels has high predictive value for pregnancy outcomes in PE patients. 2025 Vol. 31 (10): 1696-1702 [Abstract] ( 110 ) HTML (1 KB)  PDF (1351 KB)  ( 9 ) 1702 Use of Folic Acid in Pregnant Women in Early Pregnancy and the Influencing Factors XIA Yu, HOU Yanhui, LI Shen, et al DOI: 10.3969/j.issn.1006-6233.2025.10.020 Objective: To investigate the use of folic acid in pregnant women in early pregnancy and explore its influencing factors. Methods: A total of 155 pregnant women in early pregnancy in the hospital from January 2023 to January 2024 were studied. When they came to the hospital for further consultation at 12 weeks of pregnancy, the questionnaire survey was used to understand their use of folic acid in early pregnancy and general data (age, marriage age, education level, and whether they knew the best use time and effect of folic acid). The level of folic acid in red blood cell was detected, and then pregnant women with differences in using folic acid were grouped. The differences in red blood cell folic acid, pregnancy complications and fetal growth and development were compared. Multivariate Logistic regression analysis was used to screen the risk factors for poor folic acid use in pregnant women in early pregnancy. Results: Among 155 pregnant women, 63 cases had poor folic acid use, with the rate of poor folic acid use of 40.65% (63/155). The level of red blood cell folic acid in poor use group was lower than that in good use group, with a statistical significance (P<0.05). Multivariate logistic regression analysis results showed that the education level of high school or below, no pre-pregnancy examination, no awareness of the best time to take folic acid and no awareness of the effect of folic acid were the risk factors for the poor use of folic acid in pregnant women in early pregnancy, with statistical differences (P<0.05). The total number of cases with pregnancy complications was more in poor use group than that in good use group, with a statistical difference (P<0.05). The incidence rate of fetal neural tube defects in poor use group was higher, and the neonatal weight was lower, and the body length and head circumference were shorter than those in good use group, with statistical differences (P<0.05). Conclusion: The incidence rate of poor folic acid use is still high in pregnant women in early pregnancy, and risk factors include four items including low education level, no pre-pregnancy examination, no awareness of the best time to take folic acid, and no awareness of the effect of folic acid, which may increase the risk of pregnancy complications and affect the fetal growth and development. Clinically, it is necessary to carry out targeted intervention measures to improve the use of folic acid. 2025 Vol. 31 (10): 1702-1707 [Abstract] ( 125 ) HTML (1 KB)  PDF (1125 KB)  ( 6 ) 1707 Application of Bronchoalveolar Lavage Combined with mNGS in Detecting Pathogens of Secondary Pulmonary Infection in Immunosuppressed Patients ZHANG Xinxin, LUO Yuan, YANG Li DOI: 10.3969/j.issn.1006-6233.2025.10.021 Objective: To analyze the application value of BAL combined with mNGS in detecting pathogens of secondary pulmonary infection in immunosuppressed patients. Methods: The clinical data of 60 patients with immunosuppressed secondary lung infections admitted to our hospital from January 2022 to June 2024 were retrospectively analysed. All patients underwent traditional pathogenicity testing and BAL combined with mNGS testing, and the positivity rate of pathogen detection, the distribution of pathogenicity, the consistency of confirmatory results, and the diagnostic efficacy of the two testing methods were compared. Results: The positive rate of pathogens detected by BAL combined with mNGS (88.33%) was higher than traditional pathogen detection (40.00%) (P<0.05). Secondary pulmonary infections in immunosuppressed patients mainly included bacterial infection, fungal infection and viral infection, and nearly half were mixed infections. Bacterial infections were mainly caused by Streptococcus pneumoniae, Staphylococcus aureus, and Klebsiella pneumoniae. Fungal infections were mainly caused by Aspergillus and Pneumocystis. The viruses are predominantly cytomegalovirus, influenza virus, and neocoronavirus. The detection rates of bacteria, fungi and viruses by BAL combined with mNGS (41.67%, 30.00% and 48.33%) were higher than traditional pathogen detection (20.00%, 13.33% and 25.00%) (P<0.05). Compared with confirmed diagnosis results, Kappa value of BAL combined with mNGS detection was 0.838, indicating high consistency (P<0.05). Kappa value of traditional pathogen detection was 0.046, indicating low consistency (P>0.05). The sensitivity, diagnostic coincidence rate and negative predictive value of pathogen detection by BAL combined with mNGS were 98.11%, 96.67%, and 85.71%, respectively, which were all higher than those of traditional etiology, which were 41.51%, 45.00% and 13.89% respectively (P<0.05). Conclusion: BAL combined with mNGS has relatively high value in detecting pathogens of secondary pulmonary infection in immunosuppressed patients. 2025 Vol. 31 (10): 1707-1712 [Abstract] ( 99 ) HTML (1 KB)  PDF (1119 KB)  ( 180 ) 1712 Clinical Research on the Correlation of Serum Chemokine and Inflammatory Factor Levels with Perihematomatous Edema Bands and Prognosis after Cerebral Hemorrhage YU Yongbo, LIN Xiaoxiang, LI Jianxia DOI: 10.3969/j.issn.1006-6233.2025.10.022 Objective: To explore the correlation between the levels of serum chemokines and inflammatory factors and the perihematomal edema zone after intracerebral hemorrhage as well as the prognosis. Methods: A total of 103 patients were split into two groups based on their scores on the modified Rankin Scale: those with a good prognosis (n=41) and those with a poor prognosis (n=62), and the poor prognosis group was further divided into subgroups according to the size of the perihematomal edema zone. The levels of serum chemokines CCL12, CCL3, CCL17 and inflammatory factors IL-6, TNF-α and IL-1β in the patients were detected, and the relationships between these factors and the volume of the edema zone as well as the prognosis were analyzed. Results: Significantly higher concentrations of serum chemokines CCL12, CCL3, CCL17, together with inflammatory mediators IL-6,TNF-α and IL-1β, were detected in the poor prognosis group than those in the good prognosis group(P<0.05). In the poor prognosis group, the levels of serum inflammatory factors in the subgroup with a larger perihematomal edema zone were higher than those in the subgroup with a smaller edema zone (P<0.05). The levels of serum chemokines CCL12, CCL3, CCL17 and inflammatory factors IL-6 and IL-1β were positively correlated with the volume of the perihematomal edema zone ( P<0.05), and they were also independent risk factors affecting the size of the volume of the edema zone (P<0.05). Conclusion: The levels of serum chemokines and inflammatory factors can serve as potential biomarkers for evaluating the degree of perihematomal edema and the prognosis in patients with intracerebral hemorrhage. An increase in their levels is closely associated with the expansion of the edema zone and a poor prognosis. 2025 Vol. 31 (10): 1712-1717 [Abstract] ( 107 ) HTML (1 KB)  PDF (1125 KB)  ( 5 ) 1718 Correlation of the Levels of Neuron-Specific Enolase and Interleukin with the Degree of EEG Abnormalities in Patients with Status Epilepticus BAI Wenting, SUN Chang, CHEN Jingmin, et al DOI: 10.3969/j.issn.1006-6233.2025.10.023 Objective: To observe the correlation of the levels of neuron-specific enolase (NSE) and interleukin-6 (IL-6) with the degree of EEG abnormalities in patients with status epilepticus. Methods: A total of 103 patients with status epilepticus (status epilepsy group) admitted to Inner Mongolia Xingan League People's Hospital from January to December 2023, and 100 healthy individuals (control group) who received health check-ups during the same period were enrolled in the study. Serum NSE and IL-6 levels in the two groups were compared. Patients in the status epilepsy group were divided into the normal group, the mild abnormality group, the moderate abnormality group, and the severe abnormality group based on EEG examination results. Univariate and multivariate analyses were conducted to evaluate the main influencing factors of EEG abnormalities in patients with epilepsy. Serum NSE and IL-6 levels in patients with different degrees of EEG abnormalities were compared. The correlation between serum NSE and IL-6 levels and the degree of EEG abnormalities was analyzed. Results: Serum NSE and IL-6 levels were higher in the status epilepsy group than in the control group (P<0.05). There were significant differences in epileptic seizure frequency and seizure type, and serum NSE and IL-6 levels among patients with different degrees of EEG abnormalities (P<0.05). Seizure frequencies and serum NSE and IL-6 levels in the severe abnormality group, the moderate abnormality group, the mild abnormality group, and the normal group decreased in order (P<0.05). The proportion of generalized seizures in the severe abnormality group was higher than those in the other three groups (P<0.05). and the proportion of generalized seizures in the moderate abnormality group was higher than those in the normal group and the mild abnormality group (P<0.05). Multivariate analysis found that epileptic seizure frequency, seizure type, serum NSE, and IL-6 levels were independent influencing factors of EEG abnormalities in patients with epilepsy (P<0.05). Spearman correlation analysis found that serum NSE and IL-6 levels in patients with status epilepticus were positively correlated with the degree of EEG abnormalities (P<0.05). Conclusion: The degree of EEG abnormality is related to epileptic seizure frequency, seizure type, NSE, and IL-6 in patients with status epilepticus. Moreover, serum NSE and IL-6 levels are positively correlated with the degree of EEG abnormality, which deserves close attention. 2025 Vol. 31 (10): 1718-1723 [Abstract] ( 112 ) HTML (1 KB)  PDF (1240 KB)  ( 6 ) 1723 Expression Levels of Serum NT-proBNP cTnI and CysC in Patients with Cardiac Valve Replacement and Predictive Value on Prognosis WU Hao, CHEN Mingbao, MENG Lei, et al DOI: 10.3969/j.issn.1006-6233.2025.10.024 Objective: To analyze serum N-terminal pro-B-type natriuretic peptide (NT-proBNP), cardiac troponin I (cTnI) and cystatin C (CysC) expression levels in patients receiving cardiac valve replacement and explore the predictive value on prognosis. Methods: Totally 192 patients who received cardiac valve replacement in Xuzhou Central Hospital from February 2022 to July 2023 were enrolled in the study. The above patients were classified into 147 cases in the good prognosis group and 45 cases in the poor prognosis group by means of the postoperation Serum NT-proBNP, cTnI and CysC levels were measured in all study subjects after admission. Binary Logistic regression analysis was performed to construct a prediction model with NT-proBNP, cTnI and CysC as variables. The predictive value of postoperation serum NT-proBNP, cTnI and CysC on the prognosis was analyzed by receiver operating characteristic curve (ROC). Results: Compared with the good prognosis group, the levels of serum NT-proBNP, cTnI and CysC in the poor prognosis group were enhanced significantly (P<0.05). Logistic regression analysis suggested that NT-proBNP, cTnI and CysC were independent predictors of poor prognosis in patients with cardiac valve replacement. The prediction model was manifested as logit(P)=-11.269 + 0.005 × NT-proBNP + 9.479 × cTnI + 1.508 CysC. ROC curve indicated that the area under the curve (AUC), sensitivity and specificity of combination of NT-proBNP, cTnI and CysC in predicting the poor prognosis were 0.880, 75.56% and 83.67%. The efficiency of combined prediction was better than that of each indicator alone (P<0.05). Conclusion: Serum levels of NT-proBNP, cTnI and CysC in patients receiving cardiac valve replacement are closely related to the prognosis. Postoperation determination of NT-proBNP, cTnI and CysC can provide auxiliary reference value for the prognosis prediction in patients with cardiac valve replacement, so as to guide clinical active prevention and treatment measures. 2025 Vol. 31 (10): 1723-1728 [Abstract] ( 104 ) HTML (1 KB)  PDF (1295 KB)  ( 7 ) 1728 The Relationship between the Number of Circulating Tumor Cells in Peripheral Blood and Postoperative Recurrence and Metastasis of Renal Carcinoma SONG Dianbin, WANG Zhiyong, LI Xiuming, et al DOI: 10.3969/j.issn.1006-6233.2025.10.025 Objective: To investigate the number of peripheral blood circulating tumor cells (CTCS) in the selection of laparoscopic radical nephrectomy (RLRN) or open radical nephrectomy (ORN) for renal carcinoma and its relationship with postoperative recurrence and metastasis of renal carcinoma. Methods: A total of 73 patients who underwent radical nephrectomy for renal cell carcinoma (RCC) in the Department of Urology, Affiliated Hospital of Chengde Medical College from October 2018 to April 2020 were enrolled as the research subjects. They were divided into the CTC-positive group and CTC-negative group based on the CTC count (5 cells/3.5ml). Among them, the CTC-positive group included 20 patients who received open radical nephrectomy (ORN) and 19 patients who received robot-assisted laparoscopic radical nephrectomy (RLRN); the CTC-negative group included 18 patients who received ORN and 16 patients who received RLRN. The changes in CTC count at 1 week after surgery were compared between the groups. Meanwhile, all patients in both groups were followed up for 48-66 months, with tumor recurrence and metastasis set as the endpoint events. Results: In the CTC-positive group, regardless of whether patients underwent RLRN or ORN, the CTC level at 1 week after surgery decreased (for ORN, the difference before and after surgery was statistically significant, P<0.01; for RLRN, the difference before and after surgery was not statistically significant, P=0.0761). In the CTC-negative group, the CTC level increased after ORN (the difference before and after surgery was not statistically significant, P=0.1061), while the CTC level increased significantly after RLRN (the difference before and after surgery was statistically significant, P<0.01). The total recurrence and metastasis rate of the CTC-positive group was 64.1% (25/39), which was significantly higher than that of the CTC-negative group (26.4%, 9/34), and the difference was statistically significant (P=0.010). Conclusion: In CTC-positive patients, both surgical methods can reduce the CTC level. In CTC-negative patients, both surgical methods increase the CTC count, but the increase in CTC count after RLRN is more significant than that after ORN, indicating that CTC-negative patients may not be suitable for RLRN. The CTC level has certain potential in the selection and guidance of surgical methods for patients with RCC. At the same time, the CTC-positive group has an increased risk of recurrence and metastasis after surgery, suggesting that for patients with RCC after surgery, the prognosis can be evaluated by monitoring the number of circulating tumor cells (CTCs). 2025 Vol. 31 (10): 1728-1732 [Abstract] ( 115 ) HTML (1 KB)  PDF (1251 KB)  ( 13 ) 1732 Diagnostic Value of Serum IgM IL-6 PCT and CRP Levels for the Severity of Mycoplasma Pneumoniae Infection SUN Beibei, et al DOI: 10.3969/j.issn.1006-6233.2025.10.026 Objective: To investigate the diagnostic value of serum immunoglobulin M (IgM), interleukin-6 (IL-6), procalcitonin (PCT), and C-reactive protein (CRP) levels for the severity of Mycoplasma pneumoniae (MP) infection. Methods: From July 2023 to December 2024, 78 patients with MP infection were selected. Meanwhile, 27 healthy individuals were selected as the control group. The levels of IgM, IL-6, PCT and CRP in all subjects were measured. The receiver operating characteristic (ROC) curves were plotted to evaluate the diagnostic value of IgM and PCT for severe MP infection. Results: Compared with the control group, the severe group and the mild group showed higher levels of IgM, IL-6, PCT and CRP (P<0.05). Compared with the mild group, the severe group demonstrated higher levels of IgM and PCT (P<0.05). No statistically significant differences in IL-6 and CRP levels were observed between the mild group and the severe group (P>0.05). There was no statistically significant difference in data such as gender and age between the mild group and the severe group (P>0.05). According to ROC curve analysis, the areas under curves of IgM and PCT for diagnosing severe MP infection were 0.721 and 0.971, respectively. The sensitivity, specificity, and accuracy of combined diagnosis were 85.71, 98.35, and 91.03, respectively. Conclusion: IgM and PCT can serve as indicators for monitoring the condition of patients with MP infection, providing a basis for clinical judgment of condition and guiding the development of corresponding treatment plan. 2025 Vol. 31 (10): 1732-1737 [Abstract] ( 121 ) HTML (1 KB)  PDF (1244 KB)  ( 10 ) 1737 The Correlation and Early Warning Value of Quantitative Parameters of Ultrasonic Left Ventricular Pressure-Strain Loop with Heart Failure Secondary to Hypertrophic Cardiomyopathy WU Jinqiao, GUO Liyan, ZHANG Shiming, et al DOI: 10.3969/j.issn.1006-6233.2025.10.027 Objective: To investigate the correlation and early warning value of quantitative parameters of ultrasonic left ventricular pressure-strain loop (PSL) with heart failure (HF) secondary to hypertrophic cardiomyopathy (HCM). Methods: A prospective study was conducted on 216 HCM patients in Linfen People's Hospital from January 2021 to June 2023. All patients underwent ultrasound PSL examination upon admission. The patients were followed up for 1 year and were divided into HF group and non-HF group according to whether they had secondary HF. Baseline data and quantitative parameters of ultrasound PSL were compared between the two groups, including left ventricular global circumferential strain (LVGCS) in parasternal short axis view, subendocardial myocardial (CS-Endo), midmyocardial (CS-Mid), subepicardial myocardial (CS-Epi), left ventricular global torsional strain (Twist), left ventricular global longitudinal strain (LVGLS), left ventricular global radial strain (LVGRS), subendocardial myocardial (LS-Endo), midmyocardial (LS-Mid), subepicardial myocardial (LS-Epi), longitudinal strain and peak strain dispersion (PSD), global work of no use (GWW), global work of use (GCW), global work index (GWI), and global work efficiency (GWE) in apical view. The correlation and early warning value of ultrasound PSL quantitative parameters with HF secondary to HCM were analyzed. Results: After a 1-year follow-up, three patients were lost to follow-up, 31 patients developed HF, and 182 patients did not develop HF. The HF group was older than the non-HF group, had a longer duration of HCM than the non-HF group, had a higher interventricular septal thickness at admission than the non-HF group, and had a lower LVEF than the non-HF group (P<0.05). The HF group had a higher GWW than the non-HF group, and a lower GCW, GWI, and GWE than the non-HF group (P<0.05). Pearson correlation analysis showed that GWW was positively correlated with interventricular septum thickness and negatively correlated with LVEF, while GCW, GWI, and GWE were negatively correlated with interventricular septum thickness and positively correlated with LVEF (P<0.05). In the partial correlation analysis, GWW, GCW, GWI, and GWE were significantly correlated with HCM-induced HF (P<0.05). ROC curve analysis showed that the AUC of GWW, GCW, GWI, and GWE for predicting HF secondary to HCM was 0.786, 0.775, 0.721, and 0.795, with sensitivities of 77.42%, 83.87%, 64.52%, and 74.19%, and specificities of 69.23%, 64.84%, 76.92%, and 73.63%. The AUC of the combined warning of GWW, GCW, GWI, and GWE was 0.913, with a sensitivity of 87.10% and a specificity of 78.02%, which was significantly better than the value of individual warning (Z=2.540, 2.671, 3.058, 2.252, P=0.011, 0.008, 0.002, 0.024). Conclusions: The quantitative parameters of ultrasound PSL, GWW, GCW, GWI, and GWE, are significantly correlated with HF secondary to HCM, and can be used as effective early warning indicators, and the combined early warning value is more reliable. 2025 Vol. 31 (10): 1737-1743 [Abstract] ( 107 ) HTML (1 KB)  PDF (1528 KB)  ( 10 ) 1743 Analysis of Surgical Effect of Cryoballoon Ablation on Paroxysmal Atrial Fibrillation and Related Factors of Postoperative Recurrence WU Fengyi, YIN Haixian, HU Jingwen, et al DOI: 10.3969/j.issn.1006-6233.2025.10.028 Objective: To study the surgical effect of cryoballoon ablation (CBA) in treating paroxysmal atrial fibrillation (PAF) and explore the risk factors of postoperative recurrence (PR). Methods: Adopting a retrospective analysis, 148 patients with PAF who received CBA in Dongguan People' s Hospital were selected from January 2021 to June 2023, and another 51 patients who adopted radiofrequency ablation (RFCA) were collected. The surgical effect was compared. The patients were followed up for 12 months after surgery, and the risk factors of PR in CBA patients were analyzed. Results: At 12 months after surgery, no statistical differences were exhibited in the PR rate and incidence rates of surgical complications between the CBA group and the RFCA group (P>0.05). The surgical time with (119.26±28.49) min in the CBA group was shorter than (175.20±31.36) min in the RFCA group (P<0.05). At 12 months of follow-up after surgery, 38 cases (25.68%) had PR. Monocyte, left atrial diameter (LAD), mean lowest temperature and time to isolation (TTI) in the PR group were higher than those in the Non-PR group while high-density lipoprotein cholesterol (HDL-C) was lower (P<0.05). After logistic regression analysis, high LAD [OR(95%CI)=3.146 (1.559~6.345)], high mean lowest temperature [OR(95%CI)=1.624 (1.061~2.485)] and long TTI [OR(95%CI)=2.593 (1.385~4.856)] were independent influencing factors of PR (P<0.05). Conclusion: The clinical efficacy and safety of CBA in the treatment of PAF are similar to RFCA, and CBA has shorter surgical time. The LAD, mean lowest temperature of CBA and TTI may be related to the PR of patients with PAF. 2025 Vol. 31 (10): 1743-1748 [Abstract] ( 116 ) HTML (1 KB)  PDF (1128 KB)  ( 5 ) 1748 Comparison of Efficacy and Complications between Modified LIFT Procedure and Incision-Thread-Drawing Procedure in the Treatment of Trans-Sphincter Anal Fistula WANG Lu, DANG Wei, QU Bo DOI: 10.3969/j.issn.1006-6233.2025.10.029 Objective: To compare efficacy and complications between modified ligation of the inter-sphincteric fistula tract (LIFT) procedure and incision-thread-drawing procedure in the treatment of trans-sphincter anal fistula. Methods: From December 2022 to June 2024, 96 patients with trans-sphincter anal fistula were selected for the study. Using random number table method, they were divided into the routine group (48 cases) and the observation group (48 cases). The routine group was treated with incision-thread-drawing procedure, while the observation group was treated with modified LIFT procedure. Comparisons were made between groups on clinical efficacy and complications. Results: The total effective rates in the two groups were both 100%. There were no statistically significant differences in anal resting pressure, maximum squeeze pressure, mean duration of motor unit potential (MUP) of internal and external anal sphincter and puborectalis between the two groups before surgery (P>0.05). Compared with preoperative measurements, anal resting pressure, maximum squeeze pressure, and mean duration of MUP of internal and external anal sphincter and puborectalis in both groups decreased at 3 months postoperatively. Additionally, the difference values of above measurements before surgery and at 3 months postoperatively were greater in the routine group than in the observation group (P<0.05). Intraoperative blood loss, wound healing time, and hospital stay of the observation group were less and shorter than those of the routine group (P<0.05). There were statistically significant differences in VAS scores of the two groups on day 1 and at 1 week, and 2 weeks postoperatively in terms of time and interaction effects (P<0.05). At 1 week and 2 weeks postoperatively, the observation group got lower VAS scores (P<0.05). The total complication rate was lower in the observation group (P<0.05), but the recurrence rate was comparable between groups (P>0.05). Conclusion: The incision-thread-drawing procedure and modified LIFT procedure both are effective. Compared with the former, the latter can reduce anal function damage, improve surgery-related indicators, and alleviate postoperative pain, with fewer complications. 2025 Vol. 31 (10): 1748-1753 [Abstract] ( 97 ) HTML (1 KB)  PDF (1128 KB)  ( 11 )

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